Standard name
Description Protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 1.0 1.0 0.92 0.91 0.91 0.85 0.83 0.75 0.71 0.99 0.99 0.99 0.98 0.97 0.99 0.95 0.95 0.94 0.93 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0.05 0.08 0.19 0.22 0.33 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 0 0 3
Bud 1 1 0 0 0 3 0 3 0 2 1 3 0 0 0 0 0 0 0 0 3 1 2 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 5 1 0 0 5 5 1 2 1 1 1 1 0 11 5 1 0 0 0 0 0 0
Cytoplasm 236 305 308 279 278 217 358 462 320 280 203 174 311 323 411 230 194 145 236 302 293 258 261 286
Endoplasmic Reticulum 0 2 1 0 0 0 1 1 0 0 0 2 2 0 2 5 12 3 0 0 1 2 2 0
Endosome 1 0 0 0 0 0 1 3 0 1 0 2 0 3 1 0 0 0 3 1 0 0 3 5
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 1
Mitochondria 5 2 0 5 0 37 20 43 70 74 89 71 1 0 1 0 0 1 0 1 2 1 2 8
Nucleus 1 1 1 0 1 4 3 4 5 2 5 4 1 2 1 7 3 0 0 1 0 0 0 1
Nuclear Periphery 2 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 0 0 1 0 0 1 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 2 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 1 3 1 1 3
Vac/Vac Membrane 1 1 1 0 4 2 28 19 24 15 16 11 0 0 1 4 3 2 0 1 0 5 4 4
Unique Cell Count 244 310 312 280 279 235 393 508 377 336 270 245 313 325 416 235 200 147 249 318 312 277 285 333
Labelled Cell Count 248 315 319 285 283 264 418 540 421 377 316 272 316 330 419 258 219 152 249 318 312 277 285 333


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.7 6.5 5.9 6.4 4.1 5.5 5.4 4.8 5.1 4.8 4.9 6.2 6.3 6.4 7.9 8.1 7.4 5.2 5.5 5.7
Std Deviation (1e-4) 0.7 1.5 2.5 1.9 1.7 1.0 1.6 1.2 1.1 1.3 0.8 0.9 1.1 1.4 1.5 1.3 1.3 1.0 1.3 1.5 1.8
Intensity Change (Log2) – – – -0.15 -0.04 -0.68 -0.24 -0.28 -0.44 -0.37 -0.45 -0.41 -0.08 -0.05 -0.04 0.28 0.31 0.18 -0.34 -0.26 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Cytoplasm 1.2 1.2 -3.8 -4.4 -4.5 -6.4 -6.8 -8.7 -9.5 0.8 0.9 0.1 -0.8 -1.4 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 4.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 5.3 0 8.8 0 10.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.5 3.1 4.2 3.4 4.0 3.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8669 1.433 1.6311 2.2771 1.8489 1.8554 2.1639 2.9809 2.636 2.4538 2.3917 2.6802 3.8217 4.4667 4.4683 4.1432 5.1573 4.5609
Actin 0.0529 0.0005 0.0188 0.046 0.0021 0.0236 0.0285 0.0012 0.0109 0.0004 0.0145 0.0054 0.0112 0.0006 0.0007 0.004 0.0712 0.0002
Bud 0.0018 0.0039 0.001 0.0065 0.0007 0.0004 0.0037 0.0056 0.001 0.0001 0.0037 0.0003 0.0002 0.0002 0.0004 0.0035 0.0002 0
Bud Neck 0.028 0.0002 0.0031 0.0017 0.0005 0.0077 0.0075 0.0006 0.0007 0.0003 0.0005 0.0066 0.0003 0.0005 0.0001 0.0003 0.0027 0.0004
Bud Periphery 0.0028 0.0042 0.0012 0.0175 0.0013 0.0006 0.006 0.0022 0.0011 0 0.0035 0.0003 0.0003 0.0001 0.0004 0.0082 0.0002 0
Bud Site 0.0093 0.0079 0.0067 0.0154 0.002 0.0015 0.0047 0.0221 0.0034 0.0001 0.0002 0.0002 0.0003 0.0025 0.0006 0.0008 0.0004 0
Cell Periphery 0.0008 0.0002 0.0003 0.0019 0.0002 0.0001 0.0006 0.0003 0.0001 0 0 0 0.0002 0.0002 0.0001 0.0001 0.0001 0
Cytoplasm 0.3347 0.5957 0.5299 0.5096 0.4362 0.6474 0.5527 0.7441 0.7361 0.7447 0.6895 0.7898 0.6315 0.6318 0.5732 0.6238 0.5615 0.7702
Cytoplasmic Foci 0.0729 0.0069 0.03 0.0145 0.0106 0.0214 0.0296 0.012 0.0181 0.0065 0.005 0.011 0.0157 0.0071 0.0072 0.0174 0.006 0.0039
Eisosomes 0.0007 0 0.0002 0.0003 0 0.0001 0.0003 0 0.0001 0.0002 0.0001 0 0.0004 0.0001 0.0001 0.0001 0.0004 0
Endoplasmic Reticulum 0.0124 0.0026 0.0032 0.0043 0.0032 0.0021 0.005 0.0027 0.0035 0.002 0.002 0.0024 0.0047 0.0037 0.0033 0.0077 0.0076 0.0019
Endosome 0.044 0.0018 0.0134 0.0166 0.076 0.015 0.0267 0.0033 0.0089 0.0024 0.0064 0.0068 0.01 0.0031 0.0052 0.0207 0.0109 0.0026
Golgi 0.0134 0.0002 0.0068 0.0138 0.0066 0.0088 0.0057 0.0003 0.002 0.0001 0.004 0.0006 0.0013 0.0003 0.0003 0.0007 0.0032 0.0001
Lipid Particles 0.0165 0.0002 0.0056 0.0056 0.0065 0.0059 0.0057 0.0002 0.0012 0.0001 0.0029 0.0008 0.0027 0.0002 0.0003 0.0002 0.0008 0.0001
Mitochondria 0.0151 0.0003 0.0029 0.0116 0.0059 0.0069 0.008 0.0007 0.0028 0.0002 0.0008 0.0004 0.0005 0.0002 0.0003 0.0009 0.001 0.0001
None 0.3223 0.3647 0.3506 0.2924 0.3843 0.2336 0.2747 0.1886 0.1926 0.2336 0.2527 0.1614 0.3076 0.3414 0.4026 0.2991 0.3066 0.216
Nuclear Periphery 0.0128 0.0009 0.0021 0.0045 0.0126 0.006 0.0119 0.0018 0.0028 0.0016 0.0028 0.0013 0.0026 0.0013 0.001 0.0025 0.0145 0.0008
Nucleolus 0.0023 0.0004 0.0004 0.0007 0.0016 0.0003 0.0008 0.001 0.0002 0.0003 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0003 0
Nucleus 0.0099 0.003 0.0032 0.0043 0.0071 0.0107 0.0075 0.0069 0.0037 0.0045 0.0078 0.0064 0.0016 0.0038 0.0017 0.0033 0.0043 0.0023
Peroxisomes 0.019 0.0002 0.0127 0.0016 0.0015 0.0011 0.0093 0.0003 0.0042 0.0001 0.0005 0.0009 0.0011 0.0002 0.0002 0.0016 0.0004 0
Punctate Nuclear 0.0193 0.0046 0.0045 0.026 0.0169 0.0031 0.0072 0.004 0.0049 0.0018 0.0018 0.0043 0.0046 0.0013 0.0007 0.0015 0.0064 0.0005
Vacuole 0.0065 0.0016 0.0026 0.004 0.0162 0.0029 0.0029 0.0018 0.0013 0.0008 0.0008 0.0007 0.0021 0.0012 0.0013 0.0028 0.0007 0.0006
Vacuole Periphery 0.0027 0.0001 0.0009 0.0012 0.0081 0.001 0.0011 0.0002 0.0004 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0006 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.2368 29.2983 25.364 27.7273 23.6075 20.0177 32.915 35.5446 22.9026 27.8792
Translational Efficiency 0.8977 1.0515 1.1619 0.7025 1.0267 1.7293 1.0622 0.844 1.3918 1.1177

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘