Standard name | |
---|---|
Human Ortholog | |
Description | Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 8 | 1 | 1 | 2 | – | 2 | 6 | 6 | 9 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 2 | 2 | 3 | 9 | – | 9 | 8 | 22 | 23 | 26 | 2 | 0 | 3 | 3 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 370 | 283 | 584 | 447 | 505 | 665 | – | 660 | 531 | 593 | 517 | 587 | 644 | 809 | 887 | 235 | 263 | 245 | 352 | 270 | 559 | 360 | 484 | 512 |
Endoplasmic Reticulum | 1 | 1 | 0 | 3 | 4 | 7 | – | 11 | 9 | 14 | 11 | 22 | 7 | 5 | 3 | 8 | 8 | 4 | 0 | 0 | 4 | 1 | 3 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 0 | 1 | 0 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 2 |
Mitochondria | 2 | 2 | 6 | 19 | 24 | 84 | – | 120 | 209 | 243 | 232 | 341 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 2 | 2 | 2 | 2 | 4 | 2 | – | 25 | 16 | 32 | 28 | 37 | 0 | 5 | 3 | 9 | 6 | 11 | 3 | 4 | 8 | 8 | 5 | 7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 10 | 5 | 10 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 4 | 3 | 42 | 47 | – | 246 | 99 | 127 | 55 | 38 | 3 | 2 | 5 | 24 | 17 | 33 | 1 | 2 | 7 | 2 | 5 | 6 |
Unique Cell Count | 374 | 286 | 592 | 463 | 558 | 767 | 1002 | 763 | 905 | 750 | 924 | 650 | 816 | 893 | 262 | 282 | 268 | 366 | 290 | 599 | 382 | 513 | 545 | |
Labelled Cell Count | 378 | 291 | 606 | 477 | 583 | 817 | 1086 | 884 | 1049 | 878 | 1064 | 658 | 822 | 902 | 284 | 304 | 296 | 366 | 290 | 599 | 382 | 513 | 545 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.6 | 13.2 | 7.3 | 8.9 | 8.9 | 7.9 | – | 7.7 | 7.1 | 6.9 | 6.8 | 6.5 | 12.4 | 13.3 | 13.0 | 14.5 | 14.4 | 13.1 | 11.0 | 12.1 | 12.4 |
Std Deviation (1e-4) | 1.7 | 2.5 | 1.5 | 1.6 | 1.6 | 1.5 | – | 1.6 | 1.7 | 1.5 | 1.6 | 1.4 | 2.3 | 2.3 | 2.1 | 3.8 | 3.3 | 3.4 | 2.0 | 2.4 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.29 | 0.29 | 0.11 | – | 0.08 | -0.04 | -0.07 | -0.09 | -0.15 | 0.77 | 0.88 | 0.84 | 1.0 | 0.98 | 0.85 | 0.6 | 0.74 | 0.77 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | -1.0 | -1.4 | -0.2 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 3.2 | 3.7 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -6.2 | -8.0 | – | -15.3 | -13.9 | -15.3 | -14.1 | -15.9 | 0.7 | 0.9 | 1.3 | -6.1 | -4.3 | -5.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 3.0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 3.5 | 7.3 | – | 7.8 | 13.2 | 13.1 | 14.2 | 16.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 3.2 | 2.8 | 4.1 | 4.2 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.9 | 5.2 | – | 12.7 | 8.5 | 8.9 | 5.9 | 4.0 | 0 | 0 | 0 | 6.4 | 4.8 | 7.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0128 | 9.1985 | 7.7113 | 7.019 | 7.2937 | 7.9621 | 5.8143 | 7.6201 | 6.7232 | 6.2648 | 5.4759 | 6.7634 | 7.7113 | 10.1178 | 8.3814 | 8.2655 | 8.7838 | 8.4214 |
Actin | 0.006 | 0.0004 | 0.0045 | 0.0096 | 0.0018 | 0.0051 | 0.0032 | 0.0018 | 0.0146 | 0.0141 | 0.0033 | 0.0018 | 0.0397 | 0.0005 | 0.0196 | 0.007 | 0.0214 | 0.0024 |
Bud | 0.0006 | 0.0002 | 0.0009 | 0.0011 | 0.0001 | 0.0002 | 0.0014 | 0.0022 | 0.0023 | 0.001 | 0.0015 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0002 | 0.0009 | 0.0001 |
Bud Neck | 0.0014 | 0.0002 | 0.0007 | 0.0155 | 0.0007 | 0.0014 | 0.0016 | 0.0002 | 0.0009 | 0.0015 | 0.0009 | 0.0012 | 0.0038 | 0.0002 | 0.0008 | 0.0008 | 0.0017 | 0.0125 |
Bud Periphery | 0.0007 | 0 | 0.0006 | 0.0011 | 0.0001 | 0.0002 | 0.0004 | 0.001 | 0.0031 | 0.0009 | 0.0023 | 0.0001 | 0.0015 | 0 | 0.0006 | 0.0003 | 0.0007 | 0.0001 |
Bud Site | 0.0018 | 0.0007 | 0.0058 | 0.0176 | 0.0002 | 0.0004 | 0.0022 | 0.0099 | 0.0017 | 0.0007 | 0.0023 | 0.0001 | 0.0033 | 0.0004 | 0.001 | 0.0005 | 0.0051 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8237 | 0.9822 | 0.9195 | 0.8182 | 0.8585 | 0.9185 | 0.8476 | 0.966 | 0.9064 | 0.8651 | 0.8195 | 0.9309 | 0.7636 | 0.987 | 0.9236 | 0.8949 | 0.7483 | 0.9201 |
Cytoplasmic Foci | 0.0276 | 0.0014 | 0.0105 | 0.016 | 0.028 | 0.0065 | 0.031 | 0.0028 | 0.0076 | 0.0148 | 0.0098 | 0.0115 | 0.0261 | 0.0016 | 0.0052 | 0.0112 | 0.0515 | 0.0101 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0056 | 0.0008 | 0.0016 | 0.0017 | 0.0019 | 0.0037 | 0.0071 | 0.0012 | 0.002 | 0.0032 | 0.0044 | 0.0022 | 0.0091 | 0.0006 | 0.0039 | 0.002 | 0.0032 | 0.0039 |
Endosome | 0.0269 | 0.0005 | 0.0167 | 0.0416 | 0.0199 | 0.0182 | 0.0315 | 0.0021 | 0.0087 | 0.0247 | 0.0561 | 0.0105 | 0.0257 | 0.0002 | 0.0058 | 0.0098 | 0.0519 | 0.0172 |
Golgi | 0.0053 | 0 | 0.0058 | 0.0219 | 0.0189 | 0.0035 | 0.0105 | 0.0001 | 0.0057 | 0.0094 | 0.0122 | 0.0029 | 0.0062 | 0 | 0.0072 | 0.0155 | 0.0532 | 0.0017 |
Lipid Particles | 0.0088 | 0 | 0.0032 | 0.003 | 0.0176 | 0.0043 | 0.0099 | 0 | 0.0015 | 0.012 | 0.0021 | 0.0039 | 0.0087 | 0 | 0.0009 | 0.0064 | 0.0107 | 0.0006 |
Mitochondria | 0.0135 | 0.0001 | 0.0063 | 0.0167 | 0.0021 | 0.0039 | 0.0024 | 0.0001 | 0.0115 | 0.0047 | 0.0428 | 0.0007 | 0.0097 | 0.0001 | 0.0041 | 0.0221 | 0.0093 | 0.0005 |
None | 0.0141 | 0.0021 | 0.0018 | 0.0014 | 0.0014 | 0.0027 | 0.0078 | 0.0035 | 0.0081 | 0.003 | 0.0028 | 0.004 | 0.014 | 0.0034 | 0.0078 | 0.0068 | 0.0016 | 0.0027 |
Nuclear Periphery | 0.01 | 0.0009 | 0.0014 | 0.002 | 0.0028 | 0.0059 | 0.0061 | 0.001 | 0.0026 | 0.0022 | 0.0061 | 0.002 | 0.0226 | 0.0006 | 0.002 | 0.002 | 0.0027 | 0.0029 |
Nucleolus | 0.0022 | 0 | 0.0001 | 0.0014 | 0.0001 | 0.0003 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0015 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0202 | 0.0098 | 0.0124 | 0.0206 | 0.026 | 0.0195 | 0.0167 | 0.0066 | 0.016 | 0.0217 | 0.021 | 0.0226 | 0.0339 | 0.0046 | 0.0147 | 0.0135 | 0.0232 | 0.0206 |
Peroxisomes | 0.0143 | 0 | 0.0053 | 0.0057 | 0.0126 | 0.0021 | 0.0116 | 0.0001 | 0.0023 | 0.0179 | 0.0014 | 0.0016 | 0.0139 | 0.0001 | 0.0007 | 0.0048 | 0.0098 | 0.0009 |
Punctate Nuclear | 0.0128 | 0.0003 | 0.0007 | 0.001 | 0.0066 | 0.0021 | 0.004 | 0.0006 | 0.003 | 0.0013 | 0.0014 | 0.0028 | 0.0105 | 0.0004 | 0.0006 | 0.0007 | 0.0029 | 0.0025 |
Vacuole | 0.0028 | 0.0003 | 0.0014 | 0.0025 | 0.0005 | 0.0009 | 0.0026 | 0.0006 | 0.0011 | 0.0012 | 0.0066 | 0.0006 | 0.0032 | 0.0002 | 0.0005 | 0.0005 | 0.0011 | 0.0007 |
Vacuole Periphery | 0.0016 | 0 | 0.0005 | 0.0009 | 0.0002 | 0.0007 | 0.0008 | 0.0001 | 0.0004 | 0.0004 | 0.003 | 0.0002 | 0.0014 | 0 | 0.0002 | 0.0007 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 142.3341 | 150.884 | 145.3841 | 134.5513 | 155.1463 | 128.4075 | 137.7582 | 122.9455 | 162.4468 | 136.4773 |
Translational Efficiency | 1.9461 | 1.7308 | 1.5807 | 2.2202 | 1.5468 | 2.2961 | 1.6631 | 2.1887 | 1.3741 | 1.4547 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1484 | 1017 | 242 | 1403 | 327 | 1180 | 2697 | 1826 | 1811 | 2197 | 2939 | 3229 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 950.37 | 1047.17 | 1241.45 | 1374.06 | 904.10 | 1109.69 | 1327.76 | 1306.03 | 942.02 | 1080.75 | 1320.65 | 1335.59 |
Standard Deviation | 138.40 | 178.74 | 169.24 | 233.26 | 151.58 | 170.54 | 202.37 | 220.09 | 141.99 | 177.15 | 201.25 | 228.41 |
Intensity Change Log 2 | — | 0.139934 | 0.385465 | 0.531884 | — | 0.295602 | 0.554440 | 0.530634 | — | 0.217926 | 0.470318 | 0.531275 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000342 | 0.000745 | 0.000749 | 0.001867 | 0.001786 | 0.000647 | 0.000110 | 0.000620 | 0.000603 | 0.000692 | 0.000163 | 0.001161 |
Bud Neck | 0.000547 | 0.000862 | 0.001994 | 0.004540 | 0.003427 | 0.002014 | 0.001985 | 0.007164 | 0.001067 | 0.001481 | 0.001986 | 0.006024 |
Bud Site | 0.002841 | 0.005057 | 0.003815 | 0.020120 | 0.042449 | 0.010936 | 0.003428 | 0.022001 | 0.009992 | 0.008214 | 0.003459 | 0.021183 |
Cell Periphery | 0.000105 | 0.000070 | 0.000204 | 0.000140 | 0.000613 | 0.000082 | 0.000038 | 0.000098 | 0.000197 | 0.000076 | 0.000052 | 0.000116 |
Cytoplasm | 0.808565* | 0.821694* | 0.448324* | 0.540881* | 0.675473* | 0.775039* | 0.672341* | 0.563621* | 0.784534* | 0.796636* | 0.653896* | 0.553741* |
Cytoplasmic Foci | 0.044459 | 0.029256 | 0.009679 | 0.012476 | 0.116055 | 0.063709 | 0.003748 | 0.015750 | 0.057386 | 0.047761 | 0.004236 | 0.014327 |
Eisosomes | 0.000017 | 0.000011 | 0.000019 | 0.000014 | 0.000056 | 0.000020 | 0.000004 | 0.000015 | 0.000024 | 0.000016 | 0.000005 | 0.000015 |
Endoplasmic Reticulum | 0.001346 | 0.000703 | 0.002534 | 0.000830 | 0.008171 | 0.001295 | 0.000295 | 0.001339 | 0.002578 | 0.001021 | 0.000479 | 0.001118 |
Endosome | 0.002332 | 0.002179 | 0.001134 | 0.010417 | 0.004089 | 0.004049 | 0.000790 | 0.022715 | 0.002650 | 0.003184 | 0.000818 | 0.017372 |
Golgi | 0.000376 | 0.001010 | 0.000193 | 0.004522 | 0.001687 | 0.002851 | 0.001041 | 0.010478 | 0.000613 | 0.001999 | 0.000972 | 0.007890 |
Lipid Particles | 0.000724 | 0.000796 | 0.000918 | 0.001207 | 0.001796 | 0.000923 | 0.000274 | 0.000966 | 0.000918 | 0.000864 | 0.000327 | 0.001071 |
Mitochondria | 0.000473 | 0.000462 | 0.000329 | 0.005054 | 0.002428 | 0.001762 | 0.000968 | 0.009915 | 0.000826 | 0.001160 | 0.000915 | 0.007803 |
Mitotic Spindle | 0.000330 | 0.004853 | 0.004428 | 0.096992 | 0.006901 | 0.009279 | 0.012612 | 0.122254* | 0.001516 | 0.007230 | 0.011938 | 0.111278* |
None | 0.005783 | 0.006386 | 0.006184 | 0.013087 | 0.001696 | 0.003052 | 0.012350 | 0.008503 | 0.005045 | 0.004595 | 0.011842 | 0.010495 |
Nuclear Periphery | 0.000134 | 0.000136 | 0.000631 | 0.000604 | 0.000204 | 0.000161 | 0.000515 | 0.000934 | 0.000146 | 0.000150 | 0.000525 | 0.000791 |
Nuclear Periphery Foci | 0.003781 | 0.003339 | 0.003411 | 0.002921 | 0.004275 | 0.002669 | 0.000632 | 0.002244 | 0.003870 | 0.002979 | 0.000861 | 0.002538 |
Nucleolus | 0.000282 | 0.000492 | 0.000119 | 0.000371 | 0.000760 | 0.000248 | 0.000234 | 0.001293 | 0.000368 | 0.000361 | 0.000225 | 0.000892 |
Nucleus | 0.091912 | 0.084922 | 0.444136* | 0.197073* | 0.076621 | 0.057469 | 0.265560* | 0.093125 | 0.089151 | 0.070177 | 0.280264* | 0.138290* |
Peroxisomes | 0.000220 | 0.000654 | 0.000105 | 0.001400 | 0.001567 | 0.001699 | 0.000195 | 0.003258 | 0.000463 | 0.001215 | 0.000187 | 0.002451 |
Vacuole | 0.034973 | 0.035975 | 0.070982 | 0.077856 | 0.048008 | 0.061520 | 0.022698 | 0.098707 | 0.037327 | 0.049695 | 0.026673 | 0.089648 |
Vacuole Periphery | 0.000459 | 0.000400 | 0.000113 | 0.007628 | 0.001939 | 0.000575 | 0.000182 | 0.015000 | 0.000726 | 0.000494 | 0.000176 | 0.011797 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.70 | -3.86 | -1.11 | 0.20 | 3.15 | 3.68 | 6.62 | 5.72 | 1.12 | -2.12 | -0.02 | 2.73 | 1.19 | 0.80 | -2.80 |
Bud Neck | -3.96 | -8.15 | -8.26 | -6.77 | 0.22 | 2.06 | 2.11 | 1.05 | -1.45 | -1.46 | -1.46 | -6.39 | -7.80 | -4.30 | -2.30 |
Bud Site | -3.59 | -4.48 | -4.72 | -1.97 | -1.22 | 6.37 | 8.25 | 7.12 | 1.76 | -4.57 | 1.47 | 6.70 | 2.11 | 0.63 | -5.88 |
Cell Periphery | 3.07 | -6.71 | -0.98 | -4.97 | 6.48 | 8.11 | 8.62 | 8.47 | 2.06 | -1.79 | 7.68 | 8.27 | 7.27 | -1.42 | -2.67 |
Cytoplasm | 1.41 | 26.18 | 40.91 | 35.45 | -5.87 | -5.66 | 8.72 | 8.26 | 22.08 | -0.11 | 1.22 | 38.74 | 40.49 | 39.10 | 2.86 |
Cytoplasmic Foci | 5.46 | 14.75 | 19.92 | 13.04 | 2.75 | 6.65 | 15.63 | 15.04 | 20.05 | -8.50 | 3.39 | 25.72 | 24.00 | 23.43 | -8.26 |
Eisosomes | 3.03 | -3.91 | 2.27 | -1.60 | 5.64 | 6.85 | 9.56 | 8.63 | 5.53 | -8.03 | 4.54 | 9.98 | 7.51 | 3.69 | -7.36 |
Endoplasmic Reticulum | 2.90 | -4.40 | 3.64 | 1.58 | 7.32 | 5.30 | 6.19 | 6.21 | 1.65 | 0.38 | 4.32 | 6.41 | 7.09 | 1.89 | 3.74 |
Endosome | 0.88 | 2.91 | 1.28 | 0.44 | -0.27 | 0.71 | 9.39 | 0.69 | 0.22 | -4.29 | -0.82 | 11.55 | -0.21 | 0.31 | -4.84 |
Golgi | -1.49 | 1.38 | -1.08 | 0.58 | -1.61 | -1.18 | 4.70 | 0.21 | 1.21 | -2.98 | -2.75 | 1.88 | -2.17 | 1.28 | -3.04 |
Lipid Particles | -0.18 | -5.12 | -5.77 | -3.11 | 0.67 | 3.51 | 6.56 | 4.76 | 0.15 | -6.66 | 0.52 | 3.22 | -2.15 | -2.09 | -9.92 |
Mitochondria | 0.25 | 0.32 | -1.03 | -1.38 | -1.49 | 0.58 | 1.22 | 0.43 | -0.29 | -2.02 | -0.38 | 0.50 | -1.21 | -0.87 | -2.13 |
Mitotic Spindle | -2.97 | -1.60 | -8.49 | -6.61 | -3.63 | -0.47 | -1.45 | -5.24 | -6.26 | -5.90 | -3.58 | -7.47 | -12.31 | -9.05 | -7.08 |
None | -0.02 | 0.16 | -2.42 | -2.85 | -2.52 | -2.07 | -25.97* | -9.70 | -6.72 | 8.63 | 1.12 | -9.96 | -3.65 | -6.62 | 8.27 |
Nuclear Periphery | -2.38 | -14.37 | -28.06 | -26.35 | -3.42 | 0.42 | -15.26 | -12.53 | -20.77 | 1.73 | -2.00 | -35.20 | -32.48 | -32.39 | -0.21 |
Nuclear Periphery Foci | 1.21 | -1.60 | 5.55 | 3.58 | 4.63 | 2.55 | 6.61 | 5.83 | 6.56 | -3.48 | 2.76 | 11.13 | 9.17 | 7.12 | -3.83 |
Nucleolus | -1.03 | 1.54 | -0.38 | 0.93 | -2.43 | 1.47 | 1.44 | 0.70 | -1.84 | -1.83 | -0.37 | 1.18 | -0.45 | 0.10 | -2.02 |
Nucleus | 0.46 | -20.47 | -22.64 | -21.92 | 12.33 | 2.71 | -36.67 | -9.67 | -21.53 | 39.19 | 4.94 | -55.08 | -22.78 | -29.41 | 34.81 |
Peroxisomes | -2.18 | 1.93 | -0.36 | 1.88 | -1.51 | -0.29 | 4.18 | 1.99 | 1.98 | -1.12 | -2.74 | 4.46 | 0.21 | 2.32 | -1.36 |
Vacuole | -4.92 | -7.73 | -28.12 | -23.12 | -6.77 | -4.54 | -3.09 | -23.80 | -22.03 | -26.08 | -9.47 | -11.51 | -41.13 | -31.95 | -31.19 |
Vacuole Periphery | 0.46 | 1.40 | -1.09 | -1.45 | -1.94 | 2.82 | 3.77 | -0.41 | -2.91 | -3.70 | 1.32 | 3.07 | -2.27 | -3.26 | -4.23 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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