Standard name
Description Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.97 0.91 0.87 – 0.66 0.7 0.66 0.69 0.64 0.99 0.99 0.99 0.9 0.93 0.91 0.96 0.93 0.93 0.94 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 – 0.12 0.27 0.27 0.31 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.06 – 0.25 0.13 0.14 0.07 0 0 0 0 0.09 0.06 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 1 1 1 0 0 0 0 0 0 0 0 2 1 0 0 1
Bud 0 0 8 1 1 2 – 2 6 6 9 9 0 1 0 0 0 0 0 0 2 0 1 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 2 2 3 9 – 9 8 22 23 26 2 0 3 3 8 3 0 0 0 0 0 0
Cytoplasm 370 283 584 447 505 665 – 660 531 593 517 587 644 809 887 235 263 245 352 270 559 360 484 512
Endoplasmic Reticulum 1 1 0 3 4 7 – 11 9 14 11 22 7 5 3 8 8 4 0 0 4 1 3 3
Endosome 0 0 0 0 0 0 – 0 0 0 0 2 2 0 0 1 1 0 2 0 3 0 1 0
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 2 0 1 2 2
Mitochondria 2 2 6 19 24 84 – 120 209 243 232 341 0 0 0 3 1 0 1 0 2 0 0 0
Nucleus 2 2 2 2 4 2 – 25 16 32 28 37 0 5 3 9 6 11 3 4 8 8 5 7
Nuclear Periphery 0 0 0 0 0 1 – 10 5 10 2 2 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 4 3 42 47 – 246 99 127 55 38 3 2 5 24 17 33 1 2 7 2 5 6
Unique Cell Count 374 286 592 463 558 767 1002 763 905 750 924 650 816 893 262 282 268 366 290 599 382 513 545
Labelled Cell Count 378 291 606 477 583 817 1086 884 1049 878 1064 658 822 902 284 304 296 366 290 599 382 513 545


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.6 13.2 7.3 8.9 8.9 7.9 – 7.7 7.1 6.9 6.8 6.5 12.4 13.3 13.0 14.5 14.4 13.1 11.0 12.1 12.4
Std Deviation (1e-4) 1.7 2.5 1.5 1.6 1.6 1.5 – 1.6 1.7 1.5 1.6 1.4 2.3 2.3 2.1 3.8 3.3 3.4 2.0 2.4 2.3
Intensity Change (Log2) – – – 0.29 0.29 0.11 – 0.08 -0.04 -0.07 -0.09 -0.15 0.77 0.88 0.84 1.0 0.98 0.85 0.6 0.74 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 -1.0 -1.4 -0.2 -0.7 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 3.2 3.7 3.5 0 0 0 0 0 0
Cytoplasm -2.3 -6.2 -8.0 – -15.3 -13.9 -15.3 -14.1 -15.9 0.7 0.9 1.3 -6.1 -4.3 -5.3
Endoplasmic Reticulum 0 0 0 – 0 0 3.0 0 3.8 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 3.5 7.3 – 7.8 13.2 13.1 14.2 16.2 0 0 0 0 0 0
Nucleus 0 0 0 – 3.2 2.8 4.1 4.2 4.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.9 5.2 – 12.7 8.5 8.9 5.9 4.0 0 0 0 6.4 4.8 7.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0128 9.1985 7.7113 7.019 7.2937 7.9621 5.8143 7.6201 6.7232 6.2648 5.4759 6.7634 7.7113 10.1178 8.3814 8.2655 8.7838 8.4214
Actin 0.006 0.0004 0.0045 0.0096 0.0018 0.0051 0.0032 0.0018 0.0146 0.0141 0.0033 0.0018 0.0397 0.0005 0.0196 0.007 0.0214 0.0024
Bud 0.0006 0.0002 0.0009 0.0011 0.0001 0.0002 0.0014 0.0022 0.0023 0.001 0.0015 0.0002 0.0011 0.0001 0.0006 0.0002 0.0009 0.0001
Bud Neck 0.0014 0.0002 0.0007 0.0155 0.0007 0.0014 0.0016 0.0002 0.0009 0.0015 0.0009 0.0012 0.0038 0.0002 0.0008 0.0008 0.0017 0.0125
Bud Periphery 0.0007 0 0.0006 0.0011 0.0001 0.0002 0.0004 0.001 0.0031 0.0009 0.0023 0.0001 0.0015 0 0.0006 0.0003 0.0007 0.0001
Bud Site 0.0018 0.0007 0.0058 0.0176 0.0002 0.0004 0.0022 0.0099 0.0017 0.0007 0.0023 0.0001 0.0033 0.0004 0.001 0.0005 0.0051 0.0002
Cell Periphery 0.0001 0 0.0001 0.0003 0 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.8237 0.9822 0.9195 0.8182 0.8585 0.9185 0.8476 0.966 0.9064 0.8651 0.8195 0.9309 0.7636 0.987 0.9236 0.8949 0.7483 0.9201
Cytoplasmic Foci 0.0276 0.0014 0.0105 0.016 0.028 0.0065 0.031 0.0028 0.0076 0.0148 0.0098 0.0115 0.0261 0.0016 0.0052 0.0112 0.0515 0.0101
Eisosomes 0.0001 0 0 0.0001 0 0 0 0 0 0 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0056 0.0008 0.0016 0.0017 0.0019 0.0037 0.0071 0.0012 0.002 0.0032 0.0044 0.0022 0.0091 0.0006 0.0039 0.002 0.0032 0.0039
Endosome 0.0269 0.0005 0.0167 0.0416 0.0199 0.0182 0.0315 0.0021 0.0087 0.0247 0.0561 0.0105 0.0257 0.0002 0.0058 0.0098 0.0519 0.0172
Golgi 0.0053 0 0.0058 0.0219 0.0189 0.0035 0.0105 0.0001 0.0057 0.0094 0.0122 0.0029 0.0062 0 0.0072 0.0155 0.0532 0.0017
Lipid Particles 0.0088 0 0.0032 0.003 0.0176 0.0043 0.0099 0 0.0015 0.012 0.0021 0.0039 0.0087 0 0.0009 0.0064 0.0107 0.0006
Mitochondria 0.0135 0.0001 0.0063 0.0167 0.0021 0.0039 0.0024 0.0001 0.0115 0.0047 0.0428 0.0007 0.0097 0.0001 0.0041 0.0221 0.0093 0.0005
None 0.0141 0.0021 0.0018 0.0014 0.0014 0.0027 0.0078 0.0035 0.0081 0.003 0.0028 0.004 0.014 0.0034 0.0078 0.0068 0.0016 0.0027
Nuclear Periphery 0.01 0.0009 0.0014 0.002 0.0028 0.0059 0.0061 0.001 0.0026 0.0022 0.0061 0.002 0.0226 0.0006 0.002 0.002 0.0027 0.0029
Nucleolus 0.0022 0 0.0001 0.0014 0.0001 0.0003 0.0012 0 0.0002 0.0001 0.0002 0.0001 0.0015 0 0 0 0.0001 0.0001
Nucleus 0.0202 0.0098 0.0124 0.0206 0.026 0.0195 0.0167 0.0066 0.016 0.0217 0.021 0.0226 0.0339 0.0046 0.0147 0.0135 0.0232 0.0206
Peroxisomes 0.0143 0 0.0053 0.0057 0.0126 0.0021 0.0116 0.0001 0.0023 0.0179 0.0014 0.0016 0.0139 0.0001 0.0007 0.0048 0.0098 0.0009
Punctate Nuclear 0.0128 0.0003 0.0007 0.001 0.0066 0.0021 0.004 0.0006 0.003 0.0013 0.0014 0.0028 0.0105 0.0004 0.0006 0.0007 0.0029 0.0025
Vacuole 0.0028 0.0003 0.0014 0.0025 0.0005 0.0009 0.0026 0.0006 0.0011 0.0012 0.0066 0.0006 0.0032 0.0002 0.0005 0.0005 0.0011 0.0007
Vacuole Periphery 0.0016 0 0.0005 0.0009 0.0002 0.0007 0.0008 0.0001 0.0004 0.0004 0.003 0.0002 0.0014 0 0.0002 0.0007 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 142.3341 150.884 145.3841 134.5513 155.1463 128.4075 137.7582 122.9455 162.4468 136.4773
Translational Efficiency 1.9461 1.7308 1.5807 2.2202 1.5468 2.2961 1.6631 2.1887 1.3741 1.4547

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘