Standard name
Description Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.09 0.05 0.07 0.05 0 0 0 0.13 0.2 0.25 0.17 0.15 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0 0.06 0 0.12 0.15 0.27 0.18 0.42 0.23 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.92 0.93 0.9 0.89 0.86 0.88 0.84 0.8 0.84 0.75 0.8 0.85 0.78 0.73 0.69 0.69 0.65 0.78 0.76 0.71 0.74 0.66 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 0.08 0.08 0.16 0.17 0 0 0 0 0 0 0.1 0.1 0.11 0.12 0.16 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.14 0.19 0 0.07 0.08 0.07 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Bud 1 0 0 0 0 2 4 1 5 2 1 0 0 0 1 0 0 2 3 2 1 2 5
Bud Neck 0 0 0 0 3 0 2 0 0 0 3 0 0 0 0 0 0 0 0 2 1 1 2
Bud Site 0 0 2 2 0 4 5 7 12 18 24 0 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 17 18 15 28 18 27 17 16 18 5 50 100 124 69 73 44 0 3 2 2 7 12
Endoplasmic Reticulum 2 2 2 2 0 0 0 0 0 0 0 0 1 1 18 23 22 0 0 0 1 0 0
Endosome 2 6 7 0 3 2 0 0 1 0 0 2 1 4 9 17 15 4 6 6 1 5 7
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 2 1 0 1 1
Mitochondria 65 8 1 15 7 44 62 99 87 172 102 1 3 1 22 10 13 5 4 2 2 4 12
Nucleus 303 430 285 226 281 314 345 290 410 306 352 317 385 367 285 329 226 262 416 244 169 294 326
Nuclear Periphery 0 0 0 0 0 2 2 2 2 1 5 0 1 0 0 0 1 0 1 1 0 0 2
Nucleolus 11 14 3 3 7 18 16 30 41 63 76 2 4 2 0 0 5 34 56 38 27 70 67
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 3 8 24 8 12 4 13 7 18 3 13 12 16 17 5 6 4 6 9 7 7 15 23
Vac/Vac Membrane 1 11 2 2 3 2 3 2 3 1 5 0 2 2 51 68 65 14 35 25 15 33 52
Unique Cell Count 329 464 316 254 325 356 412 362 489 406 439 371 492 500 416 476 348 337 545 342 231 443 530
Labelled Cell Count 391 497 344 273 344 410 479 455 595 585 586 384 514 519 461 528 396 337 545 342 231 443 530


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.8 6.5 5.9 6.5 5.4 5.4 4.9 5.1 4.7 4.9 6.3 6.4 6.0 6.5 6.5 6.4 5.7 6.1 6.1
Std Deviation (1e-4) 0.6 0.9 2.1 1.5 1.8 1.6 1.6 1.3 1.2 1.5 1.1 1.2 1.6 1.4 1.3 1.3 1.4 1.1 1.3 1.5
Intensity Change (Log2) -0.15 0.01 -0.28 -0.26 -0.41 -0.34 -0.47 -0.39 -0.04 -0.02 -0.11 0.0 0.0 -0.02 -0.2 -0.08 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1.9 0 3.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.4 -0.4 0.5 -0.6 -1.7 -0.8 -3.6 3.4 5.7 7.0 4.5 4.2 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.0 3.3 3.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1.1 1.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 0 6.2 7.0 0 7.8 0 9.1 0 0 0 3.8 0 3.1
Nucleus -0.5 -1.5 -0.8 -2.5 -3.6 -2.6 -5.1 -3.7 -1.9 -4.4 -5.8 -7.0 -7.0 -7.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 2.5 0 4.5 0 7.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.3 -2.1 -4.2 -2.7 0 -2.4 0 -2.9 -2.6 -2.8 -2.7 -4.4 -4.6 -4.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 6.0 6.6 7.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rco1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available