Standard name
Description Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.09 0.05 0 0.12 0.07 0 0 0 0 0.06 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 0.96 0.92 0.95 0.87 0.86 0.83 0.8 0.83 0.81 0.98 0.98 0.97 0.98 0.98 0.97 0.98 0.96 0.94 0.94 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.11 0.12 0.14 0.1 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 5 8 3 6 10 0 0 0 0 0 0 0 0 0 3 0 2
Bud 6 0 4 0 9 3 3 8 2 5 4 11 3 2 2 0 0 0 0 1 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 29 6 11 34 33 22 17 17 19 37 34 52 15 13 17 0 6 1 0 0 1 0 0 0
Cytoplasm 329 120 267 276 421 526 528 528 452 501 455 585 292 437 395 110 163 107 337 106 264 272 221 246
Endoplasmic Reticulum 0 0 3 5 13 20 69 68 64 86 56 85 4 7 10 6 5 3 1 0 5 4 2 5
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 0 1 1 1 1 3 1 11 3 21 11 1 0 0 0 0 1 0 0 1 0 0 1
Nucleus 4 0 0 2 3 3 6 5 4 13 3 5 1 1 0 0 0 0 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 1 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 13 0 3 3 6 19 4 7 0 1 1 0 2 2 1 0 2 1 3 4
Unique Cell Count 335 120 273 287 456 554 609 612 542 629 551 722 299 447 406 112 166 110 344 110 281 290 241 268
Labelled Cell Count 370 126 287 319 493 576 632 637 567 667 586 767 316 461 425 117 176 114 344 110 281 290 241 268


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.9 50.3 34.3 32.2 28.4 24.4 23.7 25.4 23.7 24.2 22.8 23.2 40.0 40.3 41.5 45.8 48.3 50.1 44.0 44.1 44.3
Std Deviation (1e-4) 8.1 11.1 7.4 6.6 6.6 5.0 4.7 5.6 5.2 5.2 5.0 4.7 8.3 9.2 7.8 13.5 14.1 13.1 10.9 12.1 12.3
Intensity Change (Log2) -0.09 -0.28 -0.49 -0.53 -0.43 -0.54 -0.51 -0.59 -0.56 0.22 0.23 0.27 0.42 0.49 0.55 0.36 0.36 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.4 1.8 0 -1.0 -1.0 -0.4 1.1 1.3 1.8 0.6 -0.8 0.1 0 -0.2 0
Cytoplasm -1.1 -3.1 -1.9 -5.1 -5.2 -6.0 -7.0 -6.2 -6.7 -0.1 0 -0.4 0.3 0.3 -0.3
Endoplasmic Reticulum 0 1.6 2.1 5.1 5.1 5.3 5.8 4.8 5.3 0 0 1.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available