Standard name
Description Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.59 0.51 0.68 0.63 0.57 0.56 0.44 0.32 0.44 0.39 0.36 0.7 0.63 0.68 0.24 0.18 0.15 0.1 0.16 0.21 0.22 0.23 0.18
Bud 0.05 0 0.14 0.1 0.08 0.1 0.07 0.08 0.06 0.08 0.06 0.05 0.07 0.11 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0.68 0.54 0.55 0.57 0.5 0.38 0.21 0.43 0.41 0.42 0.65 0.59 0.69 0.8 0.8 0.84 0.85 0.73 0.68 0.73 0.69 0.76
Cytoplasm 0 0.06 0.08 0.08 0.11 0.06 0.07 0.07 0 0.08 0.05 0 0.06 0 0.09 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.06 0.05 0.06 0 0 0 0.05 0.08 0 0.05 0.11 0.07 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Mitochondria 0.29 0.25 0.36 0.68 0.55 0.65 0.68 0.67 0.74 0.74 0.78 0.34 0.26 0.54 0.09 0.09 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.2 0.13 0.38 0.26 0.22 0.18 0.07 0.09 0.08 0.11 0.23 0.13 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 111 71 152 160 170 226 236 186 184 134 150 172 234 269 76 35 30 21 23 54 40 57 44
Bud 9 6 32 26 23 38 36 49 26 29 27 12 25 45 9 1 3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 2 1 0
Bud Site 0 0 0 0 0 1 0 2 1 0 0 0 0 1 0 0 0
Cell Periphery 130 95 122 138 169 201 207 122 177 142 175 160 221 275 254 160 165 177 108 176 133 173 187
Cytoplasm 4 8 17 20 32 23 37 41 17 27 21 10 23 16 28 15 22 0 1 6 0 2 3
Endoplasmic Reticulum 12 8 11 15 4 14 23 30 34 9 20 26 25 11 12 10 8 5 4 4 1 6 2
Endosome 0 0 0 0 0 0 0 2 0 0 1 0 0 0 1 0 0 1 1 0 0 2 1
Golgi 3 0 5 0 4 1 1 9 0 0 0 4 3 0 19 17 7 0 1 1 0 0 0
Mitochondria 54 35 80 171 162 261 366 386 305 254 327 84 99 215 29 17 13 1 0 3 1 1 1
Nucleus 0 0 0 1 2 2 0 4 0 1 0 0 0 1 0 0 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 11 2 45 34 112 102 120 106 27 31 32 26 87 53 0 1 1 0 0 2 0 0 1
SpindlePole 1 0 0 0 0 0 1 3 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 0 1 0 2 3 0 1 1 3 1 3 0 1 1 0 0 0
Unique Cell Count 187 140 224 253 296 400 538 577 414 345 417 247 374 397 316 199 196 209 149 259 184 250 246
Labelled Cell Count 335 225 467 565 679 869 1028 943 771 631 756 494 718 887 432 257 254 209 149 259 184 250 246


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 9.4 8.1 7.6 8.8 8.1 7.8 7.6 6.8 6.4 6.5 9.0 8.9 8.0 14.4 15.9 16.3 9.2 9.1 9.5
Std Deviation (1e-4) 1.2 1.4 2.6 1.4 1.7 1.4 1.4 1.6 1.2 1.1 1.3 1.3 1.4 1.5 3.9 3.8 4.6 1.3 1.6 1.5
Intensity Change (Log2) -0.11 0.11 -0.01 -0.07 -0.1 -0.27 -0.34 -0.32 0.14 0.12 -0.02 0.82 0.97 1.0 0.18 0.16 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 -2.4 -2.8 -6.0 -9.2 -5.7 -6.8 -7.7 0.4 -1.3 0 -10.2 -10.4 -10.8
Bud -1.3 -2.4 -1.8 -3.3 -2.4 -3.4 -2.2 -3.3 -3.5 -3.1 -1.1 -4.9 -5.3 -4.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.6 -1.0 -4.1 -9.2 -2.8 -3.1 -3.0 2.3 1.1 3.7 6.5 5.6 6.5
Cytoplasm 0.1 1.2 -0.9 -0.3 -0.2 -1.9 0.1 -1.3 -1.7 -0.7 -1.9 0.5 0 1.3
Endoplasmic Reticulum 0.5 -2.4 -0.9 -0.4 0.2 1.6 -1.5 -0.1 2.3 0.9 -1.4 -0.6 0.1 -0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 2.1 2.9 0.8
Mitochondria 7.0 4.3 7.1 8.2 8.0 9.4 9.0 10.7 -0.4 -2.4 4.4 -7.6 -6.6 -7.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.0 4.4 1.5 0.7 -0.6 -5.2 -3.8 -4.6 -2.9 0.9 -2.2 -8.3 -6.5 -6.4
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.883 7.2655 7.1967 5.4322 6.1464 7.0255 7.0618 7.8543 7.442 6.4105 7.179 7.2612 6.7237 6.8945 7.5445 6.9896 6.2693 7.1876
Actin 0.0706 0.015 0.0252 0.023 0.0651 0.008 0.0558 0.0106 0.0137 0.0142 0.0059 0.0088 0.0374 0.0099 0.0336 0.0181 0.0211 0.0061
Bud 0.0009 0.0004 0.0008 0.0011 0.0014 0.0004 0.0013 0.0003 0.0004 0.0006 0.0005 0.0004 0.0013 0.0006 0.0078 0.0137 0.0005 0.0002
Bud Neck 0.0054 0.0071 0.0086 0.0064 0.0126 0.0132 0.0047 0.0047 0.0089 0.0148 0.0072 0.0024 0.0045 0.0044 0.0063 0.0094 0.0037 0.0052
Bud Periphery 0.0059 0.0029 0.0086 0.0094 0.0257 0.0085 0.008 0.0023 0.0052 0.006 0.0102 0.0075 0.012 0.0031 0.0079 0.0281 0.0088 0.0035
Bud Site 0.0061 0.1154 0.0267 0.006 0.0165 0.0034 0.0106 0.0741 0.014 0.0052 0.0026 0.0009 0.0363 0.0656 0.0116 0.02 0.0048 0.001
Cell Periphery 0.4652 0.6076 0.6869 0.6391 0.7209 0.736 0.5618 0.6477 0.7399 0.7592 0.7004 0.8222 0.5324 0.5803 0.6692 0.7408 0.5785 0.7904
Cytoplasm 0.0224 0.0499 0.0422 0.0652 0.0363 0.0256 0.0286 0.0518 0.0266 0.0495 0.018 0.0239 0.0199 0.0881 0.0393 0.0371 0.0211 0.0204
Cytoplasmic Foci 0.0052 0.0005 0.001 0.0154 0.0005 0.0028 0.0065 0.0002 0.0006 0.0056 0.0017 0.0007 0.0026 0.0004 0.0051 0.0004 0.0012 0.0001
Eisosomes 0.2735 0.1834 0.1675 0.066 0.0897 0.1558 0.2353 0.2016 0.1728 0.0383 0.1877 0.1178 0.3049 0.237 0.1862 0.1156 0.2363 0.1648
Endoplasmic Reticulum 0.0107 0.0025 0.006 0.0107 0.0058 0.0082 0.0055 0.0023 0.0032 0.0073 0.0044 0.0032 0.0027 0.0025 0.0024 0.0091 0.0055 0.0046
Endosome 0.0161 0.0001 0.0043 0.0289 0.0008 0.0054 0.0083 0.0001 0.0007 0.0319 0.0126 0.0024 0.0078 0 0.0032 0 0.0103 0.0001
Golgi 0.0058 0.0008 0.0042 0.0133 0.0038 0.0047 0.0078 0.0002 0.0031 0.0179 0.0097 0.0027 0.0025 0.0005 0.0095 0.0012 0.0252 0.0005
Lipid Particles 0.0041 0.0006 0.001 0.0333 0.0007 0.0031 0.014 0.0002 0.0007 0.0129 0.0033 0.002 0.0061 0.0006 0.0007 0.0006 0.0058 0.0004
Mitochondria 0.0321 0.0106 0.0106 0.0181 0.0162 0.0123 0.0139 0.0014 0.0064 0.0241 0.0022 0.0014 0.002 0.0001 0.0035 0.0001 0.0115 0.0001
None 0.0059 0.0014 0.0018 0.0022 0.0012 0.0012 0.0089 0.0012 0.0013 0.001 0.0297 0.0007 0.0021 0.0048 0.0024 0.0014 0.0515 0.001
Nuclear Periphery 0.0409 0.0004 0.0016 0.0045 0.0006 0.0011 0.0068 0.0004 0.0004 0.0032 0.0012 0.0005 0.0058 0.0006 0.0004 0.0008 0.0046 0.0007
Nucleolus 0.0006 0.0001 0.0001 0.0013 0.0001 0.0011 0.0005 0 0.0001 0.0003 0.0001 0.0001 0.004 0.0001 0.0001 0.0002 0.0012 0.0001
Nucleus 0.0051 0.0001 0.0007 0.0153 0.0001 0.0007 0.0078 0.0001 0.0001 0.0005 0.0003 0.0001 0.0029 0.0002 0.0001 0.0005 0.0026 0.0001
Peroxisomes 0.0013 0.0001 0.0002 0.0103 0.0003 0.0027 0.0087 0 0.0002 0.0025 0.0003 0.0007 0.0012 0 0.0092 0 0.0003 0
Punctate Nuclear 0.0124 0 0.0002 0.0039 0 0.0037 0.0016 0 0 0.0007 0.0005 0.0004 0.0092 0.0001 0.0004 0 0.0027 0.0001
Vacuole 0.0041 0.001 0.0016 0.0232 0.0017 0.0017 0.0027 0.0006 0.0014 0.003 0.0012 0.0011 0.0018 0.0008 0.0009 0.0027 0.002 0.0008
Vacuole Periphery 0.0056 0 0.0002 0.0034 0.0002 0.0004 0.0008 0 0.0002 0.0015 0.0004 0.0001 0.0006 0 0.0001 0.0001 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8832 17.791 13.4556 23.2441 23.0744 17.9783 20.2371 20.6631 21.0235 27.1439
Translational Efficiency 1.3958 1.3152 1.5524 1.1311 1.2855 1.4156 0.9292 1.1093 1.2479 1.1529

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency