Standard name
Human Ortholog
Description Subunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.96 0.96 0.93 0.86 0.71 0.64 0.66 0.56 0.57 1.0 0.99 0.99 0.91 0.94 0.94 0.96 0.94 0.91 0.88 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.31 0.11 0.38 0.46 0.6 0.55 0.65 0.64 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 1 1 2 0 0 0 0 0 0 1 0 1 2 2 1
Bud 2 1 10 0 1 2 6 8 10 9 19 0 0 1 0 0 0 0 0 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5 8 1 5 8 7 10 8 10 18 12 6 5 6 3 7 4 0 0 0 0 0 0
Cytoplasm 520 295 225 333 380 405 431 285 345 268 292 161 359 322 101 112 82 532 290 217 103 253 228
Endoplasmic Reticulum 3 0 1 0 2 3 3 1 6 9 2 0 1 2 3 2 0 1 0 0 0 4 4
Endosome 2 0 0 0 8 1 3 1 4 1 4 0 0 1 0 0 0 1 1 2 0 4 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 3 4 0 3 2 3
Mitochondria 4 2 20 109 45 177 280 266 284 310 328 1 0 2 3 3 2 2 3 6 1 1 1
Nucleus 0 0 1 0 3 0 3 2 2 0 1 0 0 0 1 3 3 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 7 3 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 4 0 0 9 4 28 16 13 18 7 0 0 3 7 2 5 8 1 3 1 2 6
Unique Cell Count 530 299 235 348 410 471 603 444 520 476 513 161 361 326 111 119 87 555 308 239 117 276 256
Labelled Cell Count 539 312 259 449 456 599 772 591 676 636 671 168 366 337 120 129 96 555 308 239 117 276 256


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 7.5 4.9 5.2 5.9 5.0 5.0 4.5 4.8 4.5 4.4 7.0 7.2 7.2 9.8 8.6 9.0 7.2 7.3 7.5
Std Deviation (1e-4) 4.7 1.1 1.0 1.6 1.5 1.1 1.4 1.2 1.2 1.3 1.2 0.9 1.2 1.3 2.0 1.9 1.8 1.8 1.4 1.5
Intensity Change (Log2) 0.08 0.27 0.03 0.03 -0.13 -0.03 -0.11 -0.16 0.52 0.57 0.55 1.01 0.81 0.88 0.56 0.58 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.6 -3.9 -7.7 -9.0 -8.7 -10.7 -10.7 2.7 3.1 2.3 -1.8 -0.7 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.5 1.0 8.1 10.3 0 0 0 0 -3.4 -5.6 -4.8 -2.0 -2.2 -2.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.4 2.9 0 3.0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5465 2.0097 1.7795 1.3336 1.5336 1.9487 1.1148 1.7434 1.5575 1.3675 1.2801 1.6619 1.7093 2.9408 2.2538 1.9432 2.6661 2.4114
Actin 0.0154 0.0003 0.0143 0.0052 0.0193 0.0058 0.0233 0.0009 0.0112 0.0027 0.0108 0.0041 0.0087 0.0002 0.0001 0.0005 0.0018 0.0002
Bud 0.0008 0.0002 0.0008 0.0006 0.0066 0.0005 0.0015 0.0009 0.0057 0.009 0.0004 0.0006 0.0002 0.0001 0.0001 0.0003 0.0005 0.0001
Bud Neck 0.0026 0.0002 0.0008 0.0005 0.0052 0.0011 0.0051 0.0002 0.0026 0.0016 0.0004 0.0006 0.0011 0.0001 0.0001 0.0002 0.0007 0.0004
Bud Periphery 0.0017 0.0002 0.0007 0.0004 0.0055 0.0004 0.0033 0.0004 0.0028 0.0216 0.0005 0.0006 0.0002 0.0001 0.0001 0.0003 0.0004 0.0001
Bud Site 0.0029 0.0013 0.0049 0.0012 0.0113 0.0003 0.0217 0.0026 0.0122 0.0037 0.0008 0.0003 0.0011 0.0012 0.0002 0.0002 0.0004 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0007 0.0001 0.0016 0.0003 0.0001 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0
Cytoplasm 0.6418 0.8436 0.7432 0.7695 0.7651 0.7921 0.7005 0.8802 0.7702 0.7394 0.6577 0.8672 0.6694 0.8563 0.783 0.7402 0.884 0.8021
Cytoplasmic Foci 0.0371 0.0196 0.0278 0.0294 0.0256 0.021 0.0318 0.0265 0.0283 0.0352 0.0373 0.0204 0.0199 0.0066 0.0063 0.0111 0.0133 0.0063
Eisosomes 0.0002 0.0001 0.0002 0.0002 0.0002 0 0.0015 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0069 0.0015 0.0023 0.0022 0.0057 0.0031 0.0106 0.0022 0.0068 0.0037 0.0019 0.0036 0.006 0.0019 0.0023 0.0127 0.0022 0.0015
Endosome 0.0341 0.0112 0.0151 0.0227 0.027 0.021 0.0253 0.0169 0.0308 0.0566 0.0342 0.0153 0.0082 0.0051 0.0035 0.0084 0.013 0.0041
Golgi 0.0088 0.0005 0.0017 0.0026 0.0048 0.0021 0.0067 0.0014 0.0058 0.0164 0.0195 0.0019 0.0018 0.0003 0.0004 0.0003 0.0005 0.0002
Lipid Particles 0.0162 0.0001 0.0032 0.006 0.0019 0.0014 0.0097 0.0003 0.0017 0.0008 0.0297 0.0013 0.0045 0.0001 0.0001 0.0005 0 0
Mitochondria 0.0033 0.0003 0.0018 0.0354 0.0035 0.001 0.0083 0.0005 0.0048 0.0094 0.032 0.0095 0.0008 0.0001 0.0002 0.0003 0.0013 0.0002
None 0.1927 0.1149 0.1654 0.1004 0.0751 0.1343 0.1285 0.0594 0.1004 0.0867 0.1267 0.0622 0.2576 0.124 0.201 0.2167 0.0782 0.1825
Nuclear Periphery 0.0071 0.0005 0.0032 0.0031 0.0231 0.0023 0.0025 0.0006 0.0021 0.0011 0.0082 0.0015 0.0025 0.0004 0.0003 0.001 0.0004 0.0002
Nucleolus 0.0024 0.0001 0.0003 0.0002 0.0008 0.0001 0.0005 0.0003 0.0003 0.0002 0.0004 0.0001 0.0032 0.0001 0 0.0001 0 0
Nucleus 0.0046 0.0013 0.0042 0.002 0.0059 0.001 0.0017 0.001 0.0016 0.0017 0.0062 0.0013 0.0048 0.0007 0.0006 0.0009 0.0009 0.0005
Peroxisomes 0.0077 0.0001 0.0029 0.0056 0.0028 0.0066 0.0101 0.0015 0.0021 0.0017 0.0235 0.0021 0.0029 0 0 0.0002 0.0001 0
Punctate Nuclear 0.0084 0.0005 0.0025 0.0082 0.0044 0.0031 0.0012 0.0008 0.001 0.0004 0.0048 0.0044 0.0044 0.0002 0.0001 0.0003 0.0002 0.0001
Vacuole 0.0041 0.0031 0.004 0.0031 0.0038 0.0022 0.0047 0.0027 0.0067 0.0059 0.0029 0.0021 0.0019 0.002 0.0012 0.0051 0.0016 0.0009
Vacuole Periphery 0.001 0.0004 0.0006 0.0013 0.0021 0.0007 0.0009 0.0006 0.0011 0.0018 0.0018 0.0006 0.0004 0.0002 0.0002 0.0005 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.7647 14.9451 28.8338 44.609 30.3463 26.7344 33.2441 28.5569 28.2158 41.0637
Translational Efficiency 2.6053 6.071 2.9311 1.6036 2.6164 3.2484 2.1397 3.1495 2.3788 2.391

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
188 616 2419 922 341 2037 1962 1481 529 2653 4381 2403

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 782.31 902.81 1010.48 1048.17 721.00 893.33 985.49 1036.97 742.79 895.53 999.29 1041.27
Standard Deviation 90.55 123.40 120.82 173.92 117.03 111.41 124.71 153.51 112.26 114.38 123.20 161.74
Intensity Change Log 2 0.206682 0.369228 0.422060 0.309194 0.450842 0.524303 0.256757 0.408947 0.472002

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000149 0.000546 0.000060 0.000896 0.000197 0.000468 0.000176 0.001150 0.000180 0.000486 0.000112 0.001052
Bud Neck 0.015871 0.008782 0.001047 0.002344 0.009830 0.009743 0.001362 0.002275 0.011977 0.009520 0.001188 0.002302
Bud Site 0.014786 0.037896 0.005057 0.024400 0.015005 0.021950 0.013296 0.022343 0.014927 0.025653 0.008746 0.023132
Cell Periphery 0.000181 0.000128 0.000030 0.000111 0.000191 0.000083 0.000037 0.000063 0.000188 0.000093 0.000033 0.000082
Cytoplasm 0.510158* 0.569283* 0.833929* 0.711986* 0.537743* 0.591323* 0.820022* 0.730139* 0.527940* 0.586205* 0.827701* 0.723174*
Cytoplasmic Foci 0.312589* 0.234225* 0.025499 0.078275 0.235506* 0.212588* 0.035727 0.053783 0.262900* 0.217612* 0.030079 0.063180
Eisosomes 0.000179 0.000061 0.000007 0.000024 0.000155 0.000084 0.000009 0.000020 0.000163 0.000078 0.000008 0.000022
Endoplasmic Reticulum 0.000825 0.000649 0.000359 0.000865 0.001217 0.000447 0.000277 0.000574 0.001078 0.000494 0.000323 0.000686
Endosome 0.035878 0.029118 0.001492 0.004016 0.032806 0.025205 0.002754 0.006463 0.033898 0.026113 0.002057 0.005524
Golgi 0.006418 0.008447 0.000156 0.001122 0.003792 0.009270 0.000192 0.003222 0.004725 0.009079 0.000172 0.002416
Lipid Particles 0.004866 0.003045 0.000383 0.001149 0.006547 0.001526 0.000357 0.000592 0.005950 0.001879 0.000371 0.000806
Mitochondria 0.001476 0.002056 0.000562 0.001445 0.005717 0.003087 0.000086 0.004036 0.004210 0.002848 0.000349 0.003042
Mitotic Spindle 0.001876 0.000922 0.003151 0.004507 0.001295 0.003881 0.003048 0.012149 0.001502 0.003194 0.003104 0.009217
None 0.000349 0.000488 0.002475 0.000563 0.002007 0.000621 0.001187 0.001462 0.001418 0.000590 0.001898 0.001117
Nuclear Periphery 0.000090 0.000055 0.000216 0.000215 0.000445 0.000042 0.000128 0.000165 0.000319 0.000045 0.000177 0.000184
Nuclear Periphery Foci 0.000768 0.000552 0.000748 0.003311 0.000851 0.000208 0.001558 0.001311 0.000821 0.000288 0.001111 0.002078
Nucleolus 0.001853 0.000247 0.000107 0.000097 0.001130 0.000207 0.000086 0.000134 0.001387 0.000216 0.000098 0.000120
Nucleus 0.013688 0.011799 0.069009 0.027464 0.028958 0.012770 0.036297 0.024513 0.023531 0.012544 0.054359 0.025645
Peroxisomes 0.003153 0.003611 0.000127 0.001073 0.002309 0.003171 0.000487 0.002236 0.002609 0.003273 0.000289 0.001790
Vacuole 0.071890 0.085589 0.055413 0.134668 0.112139 0.100672 0.082772 0.132400 0.097835 0.097170 0.067666 0.133270
Vacuole Periphery 0.002958 0.002501 0.000172 0.001467 0.002159 0.002657 0.000141 0.000968 0.002443 0.002621 0.000158 0.001159

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.87 5.08 -3.06 -0.85 -3.47 -1.70 0.65 -1.43 -0.54 -1.51 -2.42 2.94 -2.41 -0.85 -2.71
Bud Neck 2.56 5.54 5.22 7.05 -3.12 0.01 6.23 5.79 13.76 -2.00 1.79 8.30 7.73 15.57 -3.52
Bud Site -5.53 3.43 -1.30 4.92 -6.46 -3.19 2.04 -0.22 3.07 -2.38 -5.46 4.54 -1.05 4.76 -6.54
Cell Periphery 2.44 9.31 4.76 1.75 -9.59 6.12 8.85 8.02 6.56 -3.38 7.10 12.22 9.20 4.34 -9.72
Cytoplasm -2.78 -15.23 -7.63 -6.86 14.88 -3.83 -17.32 -10.87 -12.08 10.26 -5.00 -23.48 -13.19 -13.53 17.79
Cytoplasmic Foci 4.85 19.84 16.31 18.89 -14.06 2.09 20.06 18.28 35.48 -6.38 5.00 27.81 23.95 39.25 -14.99
Eisosomes 4.78 7.03 6.42 6.61 -9.37 3.44 8.15 7.77 6.15 -4.44 5.16 10.78 10.06 7.01 -9.26
Endoplasmic Reticulum 0.79 2.04 0.46 -0.49 -2.71 3.14 3.91 3.26 0.42 -0.85 3.28 4.25 3.08 -0.27 -1.95
Endosome 1.47 8.18 7.77 11.47 -7.30 2.11 8.90 8.27 15.99 -3.33 2.75 12.04 11.22 20.02 -5.68
Golgi -1.32 5.86 5.20 8.16 -1.91 -6.12 5.65 2.41 8.89 -2.59 -5.59 8.10 5.05 12.14 -3.03
Lipid Particles 1.11 4.01 3.25 1.47 -7.53 4.17 5.08 4.95 8.71 -3.19 4.45 6.35 5.86 3.49 -7.40
Mitochondria -0.47 1.79 0.93 1.14 -0.51 0.78 1.72 1.06 0.89 -2.40 0.63 1.85 1.19 1.54 -2.26
Mitotic Spindle 0.54 -0.56 -0.97 -2.96 -0.80 -2.09 -1.05 -3.96 -2.48 -3.51 -1.52 -1.27 -3.86 -2.77 -3.48
None -0.68 -14.10 -1.70 -0.48 15.80 1.60 0.97 0.94 -1.36 0.09 1.47 -0.73 0.85 -1.30 4.01
Nuclear Periphery 1.49 -8.39 -5.51 -9.15 2.08 2.61 1.61 1.57 -14.45 -0.49 2.74 0.61 0.95 -16.71 2.61
Nuclear Periphery Foci 0.43 -0.26 -4.47 -8.33 -7.43 4.33 -4.54 -2.45 -10.63 2.68 2.76 -2.10 -5.24 -12.63 -5.42
Nucleolus 0.97 1.03 1.04 2.04 3.37 1.68 1.88 1.83 3.27 -1.92 1.61 1.74 1.74 4.11 -0.57
Nucleus 0.81 -23.14 -6.16 -8.14 19.58 4.49 -3.37 0.37 -10.37 7.93 4.46 -13.90 -1.75 -13.77 21.04
Peroxisomes -0.35 4.65 3.55 5.15 -4.42 -1.97 5.37 1.79 3.78 -2.29 -1.50 7.26 3.59 6.16 -3.41
Vacuole -2.13 -1.43 -13.62 -13.39 -15.48 1.82 -0.72 -7.74 -14.13 -9.92 0.29 0.14 -13.37 -19.09 -18.94
Vacuole Periphery 0.68 4.13 2.37 2.58 -1.12 -1.45 7.31 5.22 8.62 -3.05 -0.49 7.63 4.64 6.67 -2.08