Standard name
Human Ortholog
Description Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.99 0.99 0.95 0.93 0.89 – 0.78 0.82 0.75 0.76 1.0 1.0 0.99 0.94 0.93 0.96 0.97 0.92 0.94 0.92 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.08 – 0.2 0.14 0.3 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.05 0 – 0 0.05 0 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 3 0 0 1 1
Bud 0 1 0 0 0 2 0 – 1 4 2 2 0 0 0 0 0 0 0 2 1 1 2 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 3 0 5 3 – 6 2 3 4 0 2 3 7 9 7 0 0 0 0 0 0
Cytoplasm 200 301 224 226 269 273 363 – 193 240 188 191 335 478 473 134 191 147 190 293 212 117 324 377
Endoplasmic Reticulum 0 6 0 2 1 0 0 – 0 2 2 12 0 2 2 6 7 3 0 1 1 0 1 1
Endosome 0 0 0 0 1 0 0 – 0 1 1 0 0 0 0 1 2 0 0 2 1 1 1 2
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 1 0 1 0 0 1 0 1 1 0
Mitochondria 0 2 0 2 3 19 33 – 49 42 76 42 0 0 0 3 3 2 2 2 2 1 4 1
Nucleus 2 2 1 0 1 4 9 – 5 11 9 12 1 2 0 7 3 2 0 1 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 0 – 2 2 3 1 0 0 0 0 2 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 – 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 0 1 0 0 2 2
Vac/Vac Membrane 0 2 2 2 13 14 18 – 11 16 3 13 1 2 0 5 15 7 1 1 1 0 1 3
Unique Cell Count 202 313 226 229 283 295 407 246 294 250 252 336 480 477 143 206 153 196 318 227 128 347 403
Labelled Cell Count 202 320 231 235 289 318 427 269 322 288 277 338 486 479 164 233 168 196 318 227 128 347 403


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.6 7.0 6.4 7.3 6.0 6.2 – 5.6 5.8 5.1 5.5 6.7 7.0 6.8 8.3 8.4 8.2 6.3 7.0 7.5
Std Deviation (1e-4) 0.6 0.9 1.1 1.4 1.5 1.2 1.5 – 1.5 1.2 1.3 1.3 0.9 1.1 1.1 1.6 1.7 1.9 1.4 1.3 1.3
Intensity Change (Log2) – – – -0.13 0.06 -0.22 -0.17 – -0.33 -0.27 -0.44 -0.34 -0.07 0.01 -0.04 0.25 0.26 0.24 -0.15 0.0 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 1.9 0
Cytoplasm -0.4 -2.6 -3.5 -4.6 – -7.0 -6.4 -7.6 -7.5 0.9 0.8 0.1 -3.0 -3.4 -2.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 3.3 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 4.4 – 7.1 5.9 9.0 6.4 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 2.5 0 2.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 2.5 2.4 – 2.4 2.8 0 2.7 0 0 0 0 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0034 0.001 0.0013 0.0012 0.0079 0.0026 0.0205 0.0017 0.0025 0.0006 0.0062 0.0044 – – – – – –
Bud 0.0001 0.0307 0.0597 0.0003 0.0003 0.0035 0.0021 0.0129 0.0137 0.0001 0.0005 0.0001 – – – – – –
Bud Neck 0.0002 0.0001 0.0004 0.0004 0.0005 0.0023 0.0012 0.0003 0.0004 0.0003 0.0015 0.0012 – – – – – –
Bud Periphery 0.0001 0.0073 0.0206 0.0002 0.0002 0.0028 0.0021 0.005 0.009 0.0001 0.0005 0.0001 – – – – – –
Bud Site 0.0006 0.0234 0.0345 0.0001 0.0016 0.0001 0.0131 0.0134 0.005 0.0002 0.0005 0.0001 – – – – – –
Cell Periphery 0.0002 0.0006 0.0008 0.0001 0.0001 0.0004 0.0002 0.0002 0.0001 0 0.0001 0 – – – – – –
Cytoplasm 0.4542 0.6735 0.4483 0.572 0.4006 0.6742 0.0291 0.4384 0.403 0.2751 0.1418 0.5045 – – – – – –
Cytoplasmic Foci 0.0457 0.0028 0.0093 0.0074 0.0855 0.0138 0.1047 0.0096 0.0094 0.0084 0.0196 0.0176 – – – – – –
Eisosomes 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0002 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0349 0.0028 0.0016 0.0011 0.0014 0.0023 0.0111 0.0026 0.0016 0.0083 0.0011 0.0017 – – – – – –
Endosome 0.0588 0.0003 0.0008 0.0016 0.0757 0.001 0.2573 0.0024 0.003 0.0164 0.0884 0.0199 – – – – – –
Golgi 0.0147 0.0001 0.0001 0.0022 0.1127 0.0001 0.0428 0.0005 0.003 0.0027 0.0632 0.0016 – – – – – –
Lipid Particles 0.031 0.0003 0.0002 0.0336 0.032 0.0002 0.0704 0.0007 0.0005 0.0095 0.004 0.001 – – – – – –
Mitochondria 0.0023 0.0005 0.0014 0.0039 0.0057 0.0003 0.0219 0.0007 0.0016 0.0256 0.0115 0.0011 – – – – – –
None 0.2805 0.2516 0.4071 0.3543 0.265 0.2847 0.2724 0.4936 0.4985 0.6282 0.6537 0.3989 – – – – – –
Nuclear Periphery 0.0286 0.0006 0.001 0.0012 0.0004 0.0012 0.032 0.0019 0.0023 0.0076 0.0007 0.0017 – – – – – –
Nucleolus 0.0006 0.0002 0.0007 0.0002 0.0001 0.0004 0.0023 0.0003 0.0006 0.0002 0.0001 0.0005 – – – – – –
Nucleus 0.0069 0.0023 0.0055 0.0018 0.0003 0.0043 0.0083 0.0084 0.0197 0.0086 0.0009 0.0217 – – – – – –
Peroxisomes 0.0262 0.0001 0.0002 0.0146 0.0073 0 0.0668 0.0026 0.0105 0.0021 0.0018 0.0022 – – – – – –
Punctate Nuclear 0.0068 0.0007 0.0052 0.0032 0.0007 0.0049 0.0257 0.004 0.0146 0.0034 0.0007 0.0204 – – – – – –
Vacuole 0.0028 0.0008 0.0011 0.0005 0.0012 0.0008 0.0107 0.0008 0.0007 0.0014 0.0019 0.001 – – – – – –
Vacuole Periphery 0.0013 0.0001 0.0002 0.0002 0.0008 0.0001 0.0052 0.0001 0.0001 0.0013 0.0013 0.0004 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.696 8.244 5.7576 8.992 7.0335 6.5062 9.1936 8.9353 11.4876 9.6617
Translational Efficiency 0.3641 0.3754 0.5947 0.4316 0.404 0.6564 0.5057 0.6161 0.444 0.4873

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant
Localization
Cell Percentages cytoplasm (68%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ypk2

Ypk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available