Standard name
Description Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.13 0.15 0.09 0.14 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.05 0.05 0.13 0.45 0.55 0.56 0.61 0.56 0.64 0.66 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.94 0.96 0.92 0.87 0.85 0.77 0.74 0.72 0.75 0.69 0.69 0.93 0.8 0.77 0.85 0.8 0.9 0.9 0.96 0.94 0.83 0.66 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0 0 0 0.07 0 0 0 0 0 0.05 0.07 0 0 0.05 0 0 0 0 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.2 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 2 4 13 7 4 6 9 1 1 0 0 0 0 5 0 0 3 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 12 9 7 18 25 14 22 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 2 5 6 17 16 15 16 21 20 29 26 22 60 60 23 29 10 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 4 5 2 0 0 0 0 0 2
Endosome 2 0 1 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 4 0 0 3 3 6
Golgi 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 1 0 3
Mitochondria 13 3 15 17 63 263 390 394 340 317 342 506 0 2 0 7 4 1 3 0 1 1 3 9
Nucleus 255 132 312 294 422 498 545 518 399 427 367 522 348 364 315 213 172 188 251 136 300 268 213 191
Nuclear Periphery 0 0 4 9 11 7 14 16 5 16 8 10 2 5 4 0 1 1 0 0 0 0 0 3
Nucleolus 1 4 4 20 21 14 24 47 25 23 14 25 3 22 27 8 8 10 2 0 3 11 26 38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 2 2 1 5 3 3 2 1 1 6 6 0 0 0 3 0 0 1 1 1
Vac/Vac Membrane 8 6 0 2 10 7 12 11 7 10 9 12 3 5 5 7 5 5 6 2 7 29 65 133
Unique Cell Count 275 140 324 318 483 584 708 704 558 571 531 761 376 455 407 251 214 208 281 143 319 324 322 402
Labelled Cell Count 286 147 341 350 547 821 1014 1027 826 845 792 1133 384 469 426 262 224 217 281 143 319 324 322 402


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.0 6.7 6.5 5.2 4.3 3.8 3.8 3.6 3.5 3.5 3.5 10.0 9.5 8.8 13.3 13.9 14.6 11.9 10.9 10.9
Std Deviation (1e-4) 1.9 1.8 1.2 1.4 1.4 1.1 0.9 1.0 1.0 0.8 0.9 0.8 2.4 2.6 2.3 4.3 5.4 4.7 2.3 2.1 1.9
Intensity Change (Log2) – – – -0.04 -0.36 -0.65 -0.83 -0.81 -0.88 -0.93 -0.95 -0.96 0.57 0.5 0.39 0.98 1.05 1.12 0.83 0.7 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.7 1.1 0.6 0.8 1.9 1.7 2.8 1.7 3.0 5.8 6.2 4.2 5.6 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 4.0 0 0 0 0 0 0 0 -4.2 -3.9 -4.4 -1.1 -1.7 -2.7
Nucleus -2.1 -4.3 -5.1 -7.7 -8.6 -9.0 -8.2 -9.5 -9.9 -2.1 -6.6 -7.3 -4.8 -6.0 -2.8
Nuclear Periphery 1.4 1.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.4 2.5 0 0 0 0 0 0 0 0 2.8 3.6 1.6 0 2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0