Standard name | |
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Human Ortholog | |
Description | Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 13 | 7 | 4 | 6 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 3 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 12 | 9 | 7 | 18 | 25 | 14 | 22 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 2 | 5 | 6 | 17 | 16 | 15 | 16 | 21 | 20 | 29 | 26 | 22 | 60 | 60 | 23 | 29 | 10 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 4 | 0 | 0 | 3 | 3 | 6 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Mitochondria | 13 | 3 | 15 | 17 | 63 | 263 | 390 | 394 | 340 | 317 | 342 | 506 | 0 | 2 | 0 | 7 | 4 | 1 | 3 | 0 | 1 | 1 | 3 | 9 |
Nucleus | 255 | 132 | 312 | 294 | 422 | 498 | 545 | 518 | 399 | 427 | 367 | 522 | 348 | 364 | 315 | 213 | 172 | 188 | 251 | 136 | 300 | 268 | 213 | 191 |
Nuclear Periphery | 0 | 0 | 4 | 9 | 11 | 7 | 14 | 16 | 5 | 16 | 8 | 10 | 2 | 5 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 |
Nucleolus | 1 | 4 | 4 | 20 | 21 | 14 | 24 | 47 | 25 | 23 | 14 | 25 | 3 | 22 | 27 | 8 | 8 | 10 | 2 | 0 | 3 | 11 | 26 | 38 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 5 | 3 | 3 | 2 | 1 | 1 | 6 | 6 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 8 | 6 | 0 | 2 | 10 | 7 | 12 | 11 | 7 | 10 | 9 | 12 | 3 | 5 | 5 | 7 | 5 | 5 | 6 | 2 | 7 | 29 | 65 | 133 |
Unique Cell Count | 275 | 140 | 324 | 318 | 483 | 584 | 708 | 704 | 558 | 571 | 531 | 761 | 376 | 455 | 407 | 251 | 214 | 208 | 281 | 143 | 319 | 324 | 322 | 402 |
Labelled Cell Count | 286 | 147 | 341 | 350 | 547 | 821 | 1014 | 1027 | 826 | 845 | 792 | 1133 | 384 | 469 | 426 | 262 | 224 | 217 | 281 | 143 | 319 | 324 | 322 | 402 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 9.0 | 6.7 | 6.5 | 5.2 | 4.3 | 3.8 | 3.8 | 3.6 | 3.5 | 3.5 | 3.5 | 10.0 | 9.5 | 8.8 | 13.3 | 13.9 | 14.6 | 11.9 | 10.9 | 10.9 |
Std Deviation (1e-4) | 1.9 | 1.8 | 1.2 | 1.4 | 1.4 | 1.1 | 0.9 | 1.0 | 1.0 | 0.8 | 0.9 | 0.8 | 2.4 | 2.6 | 2.3 | 4.3 | 5.4 | 4.7 | 2.3 | 2.1 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.36 | -0.65 | -0.83 | -0.81 | -0.88 | -0.93 | -0.95 | -0.96 | 0.57 | 0.5 | 0.39 | 0.98 | 1.05 | 1.12 | 0.83 | 0.7 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 1.7 | 1.1 | 0.6 | 0.8 | 1.9 | 1.7 | 2.8 | 1.7 | 3.0 | 5.8 | 6.2 | 4.2 | 5.6 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.4 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -3.9 | -4.4 | -1.1 | -1.7 | -2.7 |
Nucleus | -2.1 | -4.3 | -5.1 | -7.7 | -8.6 | -9.0 | -8.2 | -9.5 | -9.9 | -2.1 | -6.6 | -7.3 | -4.8 | -6.0 | -2.8 |
Nuclear Periphery | 1.4 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.4 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.6 | 1.6 | 0 | 2.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2994 | 4.712 | 4.949 | 4.5069 | 4.1748 | 4.751 | 7.8045 | 6.9604 | 6.6245 | 5.8267 | 5.7665 | 6.7437 | 4.1642 | 4.7473 | 3.7604 | 4.6314 | 3.626 | 3.9078 |
Actin | 0.0139 | 0 | 0.01 | 0.0059 | 0.0005 | 0.0009 | 0.0388 | 0 | 0.0061 | 0.0001 | 0.0004 | 0.0002 | 0.0267 | 0 | 0.005 | 0.0195 | 0.04 | 0.0026 |
Bud | 0.0007 | 0 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0005 | 0 | 0.0019 | 0.001 | 0.0031 | 0.0001 |
Bud Neck | 0.0014 | 0.0001 | 0.0017 | 0.0009 | 0.0003 | 0.0009 | 0.0013 | 0.0003 | 0.0002 | 0 | 0.0004 | 0.0009 | 0.0003 | 0 | 0.0005 | 0.0005 | 0.0008 | 0.0013 |
Bud Periphery | 0.0021 | 0 | 0.0007 | 0.0016 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.001 | 0 | 0.0015 | 0.0028 | 0.0052 | 0.0002 |
Bud Site | 0.0016 | 0.0002 | 0.006 | 0.002 | 0.0001 | 0.0001 | 0.0017 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0005 | 0.006 | 0 | 0.0093 | 0.0011 | 0.0336 | 0.0002 |
Cell Periphery | 0.0004 | 0 | 0.0009 | 0.0009 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0007 | 0.0001 |
Cytoplasm | 0.0041 | 0.0072 | 0.0086 | 0.0057 | 0.0001 | 0.0001 | 0.0148 | 0.0003 | 0.0008 | 0.0003 | 0.0044 | 0.0004 | 0.013 | 0 | 0.0039 | 0.0028 | 0.0008 | 0.0017 |
Cytoplasmic Foci | 0.0179 | 0.0003 | 0.0058 | 0.0114 | 0.0011 | 0.001 | 0.0055 | 0 | 0.0018 | 0.0011 | 0.0095 | 0.0003 | 0.0074 | 0 | 0.0026 | 0.005 | 0.0009 | 0.0067 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0012 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0004 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0003 | 0.0017 | 0.0078 | 0.0014 | 0.0002 | 0.0028 | 0.0001 | 0.0007 | 0 | 0.0025 | 0.0002 | 0.0011 | 0 | 0.0006 | 0.0064 | 0.0015 | 0.0002 |
Endosome | 0.0159 | 0.0011 | 0.0071 | 0.0279 | 0.011 | 0.0039 | 0.0053 | 0.0001 | 0.005 | 0.0005 | 0.008 | 0.0022 | 0.0062 | 0 | 0.0014 | 0.031 | 0.002 | 0.0016 |
Golgi | 0.0041 | 0 | 0.0035 | 0.0065 | 0.0028 | 0.0016 | 0.0029 | 0 | 0.0027 | 0.0001 | 0.0014 | 0.0002 | 0.0026 | 0 | 0.0008 | 0.0155 | 0.0008 | 0.0003 |
Lipid Particles | 0.014 | 0.0001 | 0.0047 | 0.0155 | 0.0164 | 0.0023 | 0.0023 | 0 | 0.0035 | 0.0004 | 0.0271 | 0.0004 | 0.003 | 0 | 0.0009 | 0.0081 | 0.0008 | 0.0002 |
Mitochondria | 0.0114 | 0.0002 | 0.0017 | 0.0098 | 0.001 | 0.0007 | 0.0131 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0.0009 | 0.0061 | 0.0003 | 0.0007 | 0.0416 | 0.0077 | 0.0006 |
None | 0.0036 | 0.0011 | 0.0015 | 0.01 | 0.0002 | 0.0003 | 0.0054 | 0 | 0.0006 | 0.0002 | 0.0022 | 0.0002 | 0.0117 | 0 | 0.0005 | 0.0053 | 0.0005 | 0.0003 |
Nuclear Periphery | 0.0404 | 0.008 | 0.0183 | 0.0292 | 0.0144 | 0.0072 | 0.0326 | 0.0067 | 0.0024 | 0.0063 | 0.0092 | 0.0068 | 0.0034 | 0.0005 | 0.0026 | 0.0234 | 0.0098 | 0.0026 |
Nucleolus | 0.0349 | 0.0309 | 0.0507 | 0.0428 | 0.0719 | 0.07 | 0.0202 | 0.0306 | 0.0455 | 0.0051 | 0.0243 | 0.0786 | 0.0068 | 0.0136 | 0.0165 | 0.0435 | 0.0366 | 0.052 |
Nucleus | 0.7902 | 0.941 | 0.8592 | 0.7889 | 0.8746 | 0.9045 | 0.8338 | 0.9597 | 0.9269 | 0.9172 | 0.8676 | 0.8987 | 0.8894 | 0.9852 | 0.9465 | 0.7804 | 0.836 | 0.9229 |
Peroxisomes | 0.0161 | 0 | 0.0024 | 0.0044 | 0.0003 | 0.001 | 0.0043 | 0 | 0.0005 | 0.0001 | 0.0231 | 0.0001 | 0.0026 | 0 | 0.0006 | 0.0018 | 0.0012 | 0.0022 |
Punctate Nuclear | 0.0166 | 0.0062 | 0.0083 | 0.0113 | 0.0013 | 0.0044 | 0.0068 | 0.0011 | 0.0011 | 0.0682 | 0.0173 | 0.0029 | 0.0107 | 0.0001 | 0.0029 | 0.0037 | 0.0006 | 0.0031 |
Vacuole | 0.0046 | 0.0028 | 0.0037 | 0.0108 | 0.0008 | 0.0003 | 0.0019 | 0.0004 | 0.0007 | 0 | 0.0009 | 0.0048 | 0.0005 | 0.0001 | 0.0006 | 0.0023 | 0.0102 | 0.0007 |
Vacuole Periphery | 0.0026 | 0.0004 | 0.0032 | 0.0055 | 0.0014 | 0.0004 | 0.0026 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0031 | 0.0071 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.8467 | 54.0269 | 49.2538 | 33.897 | 45.896 | 31.0646 | 31.9955 | 25.8084 | 21.17 | 35.4362 |
Translational Efficiency | 2.0218 | 2.0813 | 1.6231 | 1.8919 | 2.0812 | 2.1825 | 1.6582 | 1.649 | 1.9226 | 1.6741 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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