Standard name | |
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Description | Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 2 | 4 | 7 | 13 | 16 | 38 | 33 | 38 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 8 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 6 | 9 | 8 | 5 | 17 | 12 | 17 | 15 | 34 | 25 | 40 | 1 | 5 | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 |
Cytoplasm | 203 | 622 | 558 | 294 | 371 | 275 | 315 | 273 | 185 | 283 | 238 | 284 | 278 | 376 | 334 | 161 | 111 | 135 | 189 | 597 | 529 | 192 | 252 | 238 |
Endoplasmic Reticulum | 2 | 8 | 14 | 10 | 16 | 41 | 65 | 59 | 47 | 102 | 62 | 103 | 8 | 14 | 1 | 3 | 6 | 4 | 1 | 5 | 11 | 1 | 12 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 2 | 0 | 1 | 1 | 10 | 20 | 18 | 36 | 33 | 49 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 31 | 25 | 28 | 37 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 |
Vac/Vac Membrane | 0 | 1 | 5 | 0 | 12 | 3 | 12 | 21 | 9 | 8 | 7 | 15 | 1 | 2 | 1 | 0 | 2 | 2 | 0 | 8 | 1 | 1 | 4 | 6 |
Unique Cell Count | 205 | 629 | 573 | 304 | 397 | 328 | 397 | 377 | 282 | 457 | 367 | 475 | 285 | 384 | 340 | 163 | 113 | 138 | 192 | 622 | 573 | 211 | 286 | 286 |
Labelled Cell Count | 211 | 638 | 590 | 312 | 413 | 343 | 425 | 403 | 321 | 534 | 430 | 566 | 288 | 397 | 346 | 169 | 120 | 144 | 192 | 622 | 573 | 211 | 286 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 78.4 | 59.7 | 59.9 | 53.3 | 31.9 | 32.3 | 26.9 | 24.8 | 22.2 | 22.8 | 22.3 | 20.9 | 56.9 | 53.5 | 49.9 | 75.6 | 95.8 | 82.6 | 61.8 | 64.6 | 63.5 |
Std Deviation (1e-4) | 13.3 | 12.7 | 14.9 | 10.7 | 9.5 | 6.7 | 5.8 | 5.6 | 6.4 | 5.6 | 5.4 | 4.4 | 14.3 | 10.7 | 11.4 | 27.8 | 27.8 | 28.8 | 20.0 | 20.4 | 23.0 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.91 | -0.89 | -1.16 | -1.27 | -1.44 | -1.4 | -1.43 | -1.52 | -0.07 | -0.16 | -0.27 | 0.33 | 0.68 | 0.46 | 0.04 | 0.11 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 4.5 | 5.8 | 7.0 | 7.3 | 6.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.1 | -0.4 | 3.1 | 1.5 | 2.7 | 3.1 | 4.7 | 4.2 | 5.2 | 0 | -0.3 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -3.0 | -7.4 | -9.2 | -11.4 | -12.9 | -14.6 | -13.5 | -15.2 | 0.1 | 0.5 | 0.8 | 1.0 | 0.5 | 0.3 |
Endoplasmic Reticulum | 0.7 | 1.4 | 6.1 | 7.8 | 7.5 | 7.6 | 10.0 | 7.9 | 9.8 | 0.3 | 1.1 | -2.5 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 5.0 | 5.5 | 6.4 | 6.8 | 7.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 8.1 | 5.7 | 6.7 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.5 | 0 | 2.5 | 4.3 | 0 | 1.3 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |