Standard name
Description Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
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Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.08 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0.05 0.05 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.93 0.84 0.79 0.72 0.66 0.62 0.65 0.60 0.98 0.98 0.98 0.99 0.98 0.98 0.99 0.96 0.92 0.91 0.88 0.83
Endoplasmic Reticulum 0 0 0 0 0 0.12 0.16 0.16 0.17 0.22 0.17 0.22 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0.06 0.08 0.09 0.10 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.05 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 2 4 7 13 16 38 33 38 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 1 1 0 0 0 2 1 0 0 0 0 0 0 0 0 2 8 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 6 9 8 5 17 12 17 15 34 25 40 1 5 3 0 0 1 0 0 2 0 0 1
Cytoplasm 203 622 558 294 371 275 315 273 185 283 238 284 278 376 334 161 111 135 189 597 529 192 252 238
Endoplasmic Reticulum 2 8 14 10 16 41 65 59 47 102 62 103 8 14 1 3 6 4 1 5 11 1 12 11
Endosome 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 2 0 1 1 10 20 18 36 33 49 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 1 1 4 0 31 25 28 37 0 0 0 4 1 1 0 1 3 0 1 0
Nucleus 0 0 1 0 3 0 0 0 0 1 0 0 0 0 2 0 0 0 0 2 0 3 1 2
Nuclear Periphery 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 2 2
Nucleolus 0 0 0 0 1 0 0 0 0 3 1 0 0 0 3 1 0 0 0 0 0 3 5 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 1 1 4
Vac/Vac Membrane 0 1 5 0 12 3 12 21 9 8 7 15 1 2 1 0 2 2 0 8 1 1 4 6
Unique Cell Count 205 629 573 304 397 328 397 377 282 457 367 475 285 384 340 163 113 138 192 622 573 211 286 286
Labelled Cell Count 211 638 590 312 413 343 425 403 321 534 430 566 288 397 346 169 120 144 192 622 573 211 286 286

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 78.4 59.7 59.9 53.3 31.9 32.3 26.9 24.8 22.2 22.8 22.3 20.9 56.9 53.5 49.9 75.6 95.8 82.6 61.8 64.6 63.5
Std Deviation (1e-4) 13.3 12.7 14.9 10.7 9.5 6.7 5.8 5.6 6.4 5.6 5.4 4.4 14.3 10.7 11.4 27.8 27.8 28.8 20.0 20.4 23.0
Intensity Change (Log2) – – – -0.17 -0.91 -0.89 -1.16 -1.27 -1.44 -1.4 -1.43 -1.52 -0.07 -0.16 -0.27 0.33 0.68 0.46 0.04 0.11 0.08

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 4.5 5.8 7.0 7.3 6.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -0.4 3.1 1.5 2.7 3.1 4.7 4.2 5.2 0 -0.3 0 0 0 0
Cytoplasm -1.1 -4.8 -6.7 -8.2 -10.4 -12.5 -13.8 -11.9 -14.9 -0.7 -2.0 -2.8 -6.1 -6.9 -9.0
Endoplasmic Reticulum 0.7 1.4 6.1 7.8 7.5 7.6 10.0 7.9 9.8 0.3 1.1 -2.5 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 5.0 5.5 6.4 6.8 7.5 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 8.1 5.7 6.7 6.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0