Standard name
Description Dehydrogenase involved in ubiquinone and sphingolipid metabolism; oxidizes 4-hydroxybenzaldehyde into 4-hydroxybenzoic acid in ubiquinone biosynthesis; converts hexadecenal to hexadecenoic acid in sphingosine 1-phosphate breakdown pathway; located in the mitochondrial outer membrane and also in lipid particles; human homolog ALDH3A2, a fatty aldehyde dehydrogenase (FALDH) mutated in neurocutaneous disorder Sjogren-Larsson syndrome, can complement yeast hfd1 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0.06 0 0 0.05 0.05 0.05 0 0 0.08 0 0 0 0 0.05 0.06 0.13 0.13 0.1 0.12
Bud 0 0 0.06 0.05 0.05 0 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.06 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.77 0.65 0.61 0.46 0.33 0.2 0.22 0.1 0.12 0.12 0.14 0.62 0.52 0.55 0.3 0.26 0.3 0.25 0.36 0.17 0.45 0.28 0.25
Endoplasmic Reticulum 0 0.07 0.15 0.09 0.16 0.36 0.16 0.09 0.2 0.22 0.15 0.08 0.31 0.27 0 0.05 0.07 0.08 0.31 0.14 0.18 0.11 0.13 0.07
Endosome 0.07 0.08 0.06 0.13 0.21 0.07 0.25 0.21 0.05 0 0.06 0 0.1 0.2 0.4 0.4 0.42 0.38 0.07 0.11 0.14 0 0.15 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0.06 0.2 0.14 0.39 0.43 0.47 0.72 0.74 0.7 0.8 0 0 0 0.08 0 0.05 0.17 0.12 0.23 0.09 0.13 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06 0.1 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.17 0.07 0.05 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.06 0 0 0 0.05 0 0 0 0 0 0 0.06 0.27 0.31 0.23 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.1 0.17 0.06 0.08 0 0.05 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 9 1 11 20 9 16 17 26 11 8 3 6 0 2 1 0 7 10 25 6 12 12
Bud 1 1 9 10 14 8 22 16 17 19 10 7 0 1 0 1 1 0 2 3 0 0 1 2
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 5 4 0 2 3
Bud Site 0 0 0 0 0 0 2 3 4 2 3 2 0 0 0 0 0 0
Cell Periphery 3 0 4 6 18 11 13 6 5 8 0 5 5 5 3 0 0 0 0 3 3 1 1 0
Cytoplasm 112 104 106 130 136 101 94 106 33 57 25 50 45 39 42 51 27 19 34 60 35 21 34 24
Endoplasmic Reticulum 0 9 24 20 47 111 75 42 67 107 32 28 22 20 1 8 7 5 44 23 37 5 15 7
Endosome 9 11 9 27 62 21 118 100 17 5 13 3 7 15 31 69 44 24 9 17 28 1 18 15
Golgi 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2 0 2 0 4 2
Mitochondria 24 3 9 42 40 121 206 222 244 355 146 279 0 2 0 13 3 3 24 19 46 4 16 13
Nucleus 0 0 0 0 0 1 1 2 2 3 0 4 0 0 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 3 11 1 3 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 1 0 0 2 1 1 4 9 3 8 3 21 0 0 0 11 10 5 0 0 0 1 1 0
Peroxisomes 5 0 0 10 7 4 13 10 4 2 2 1 0 0 13 12 5 1 0 0 4 0 1 1
SpindlePole 3 3 11 12 5 7 18 26 7 5 6 2 3 3 5 46 33 15 1 5 4 0 4 3
Vac/Vac Membrane 1 3 1 3 9 1 16 20 6 5 7 6 0 1 1 21 11 11 8 12 5 2 4 8
Unique Cell Count 135 135 163 212 295 309 480 474 339 481 208 349 72 75 77 172 105 64 142 167 206 48 124 98
Labelled Cell Count 159 137 183 264 350 407 591 580 429 613 259 420 85 92 97 235 143 84 142 167 206 48 124 98


Endosome, Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.0 4.7 4.8 5.8 5.5 5.4 5.9 5.6 5.5 5.7 5.5 6.1 5.6 5.8 7.3 8.0 8.9 5.7 6.5
Std Deviation (1e-4) 0.9 0.7 1.0 1.2 1.4 1.1 1.1 1.7 1.1 1.2 1.3 1.4 1.1 1.2 1.8 2.3 2.0 2.8 2.8 1.9
Intensity Change (Log2) 0.03 0.29 0.22 0.18 0.31 0.23 0.21 0.25 0.21 0.36 0.25 0.3 0.61 0.75 0.91 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -0.9 0.4 0 -1.2 -0.2 -0.1 -0.1 -1.9 0 0 0 -2.2 0 0
Bud 0 -0.4 -1.6 -0.5 -1.2 -0.2 -0.9 -0.3 -2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.7 0.7 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -3.9 -6.7 -10.8 -10.0 -13.0 -13.5 -10.6 -11.6 -0.4 -1.9 -1.6 -6.5 -6.3 -4.8
Endoplasmic Reticulum 0 0.3 4.8 0.3 -2.1 1.4 2.1 0.2 -2.3 2.8 2.2 -3.2 -3.1 -2.0 -1.4
Endosome 0 4.4 0.5 5.3 4.6 -0.2 0 0.3 0 0 3.4 6.7 7.5 7.3 6.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.7 7.8 8.7 9.5 13.9 15.2 12.5 15.8 0 0 0 0.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 3.2 0 0 0 3.3 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
SpindlePole 0 -2.8 -2.4 -1.6 -0.6 -2.6 0 -1.8 0 0 0 -0.1 4.9 5.3 3.6
Vacuole 0 0 0 0 2.2 0 0 0 0 0 0 0 4.3 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hfd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available