Standard name | |
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Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 4 | 4 | 8 | 2 | 5 | 8 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 3 | 8 | 7 | 17 | 8 | 13 | 21 | 8 | 7 | 8 | 15 | 27 | 41 | 4 | 4 | 9 | 3 | 3 | 0 | 4 | 1 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 2 | 3 | 3 | 1 | 2 | 1 | 2 | 9 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 5 | 1 | 0 |
Mitochondria | 12 | 2 | 32 | 41 | 8 | 64 | 61 | 85 | 54 | 85 | 78 | 111 | 12 | 3 | 1 | 2 | 2 | 4 | 0 | 1 | 3 | 1 | 2 | 3 |
Nucleus | 17 | 32 | 59 | 87 | 136 | 116 | 192 | 267 | 127 | 156 | 134 | 183 | 188 | 170 | 173 | 26 | 12 | 28 | 37 | 59 | 57 | 44 | 125 | 155 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 5 | 5 | 3 | 4 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 3 | 7 | 18 | 12 | 47 | 59 | 110 | 48 | 79 | 84 | 93 | 32 | 26 | 38 | 9 | 1 | 1 | 4 | 7 | 7 | 13 | 37 | 52 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 6 |
Vac/Vac Membrane | 6 | 18 | 2 | 3 | 5 | 6 | 5 | 11 | 10 | 3 | 2 | 2 | 3 | 4 | 6 | 5 | 3 | 7 | 11 | 10 | 5 | 4 | 11 | 20 |
Unique Cell Count | 35 | 62 | 73 | 122 | 154 | 191 | 253 | 369 | 201 | 224 | 219 | 270 | 222 | 205 | 244 | 41 | 20 | 49 | 72 | 95 | 82 | 80 | 189 | 260 |
Labelled Cell Count | 41 | 67 | 106 | 164 | 176 | 259 | 336 | 507 | 270 | 340 | 320 | 413 | 257 | 232 | 268 | 50 | 25 | 56 | 72 | 95 | 82 | 80 | 189 | 260 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.7 | 5.2 | 5.7 | 6.7 | 5.1 | 5.3 | 5.4 | 7.1 | 5.2 | 5.2 | 5.0 | 6.9 | 7.1 | 7.0 | 9.0 | 8.9 | 8.4 | 8.8 | 7.5 | 7.5 |
Std Deviation (1e-4) | 3.2 | 1.5 | 2.1 | 2.9 | 3.2 | 2.1 | 2.0 | 2.2 | 5.4 | 2.1 | 2.0 | 1.7 | 1.5 | 2.0 | 2.2 | 2.3 | 2.5 | 2.8 | 4.2 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.36 | -0.05 | 0.03 | 0.05 | 0.44 | -0.0 | -0.01 | -0.06 | 0.39 | 0.44 | 0.42 | 0.79 | 0.76 | 0.68 | 0.74 | 0.51 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.3 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 2.1 | 2.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.4 | -7.1 | -1.6 | -3.3 | -3.7 | -2.7 | -0.9 | -1.3 | -0.4 | -8.0 | -9.4 | -10.7 | -4.4 | -2.8 | -4.2 |
Nucleus | -1.5 | 1.5 | -3.1 | -0.9 | -1.5 | -2.8 | -1.9 | -3.1 | -2.2 | 0.8 | 0.4 | -1.7 | -2.0 | -1.9 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.0 | -0.5 | 2.7 | 2.6 | 3.6 | 2.6 | 4.2 | 4.6 | 4.1 | 1.1 | 0.7 | 1.3 | 1.8 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |