Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.11 0 0.07 0.05 0.09 0 0 0.1 0 0 0 0.07 0.13 0.17 0.1 0.2 0.18 0.05 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.06 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Mitochondria 0.34 0 0.44 0.34 0.05 0.34 0.24 0.23 0.27 0.38 0.36 0.41 0.05 0 0 0.05 0.1 0.08 0 0 0 0 0 0
Nucleus 0.49 0.52 0.81 0.71 0.88 0.61 0.76 0.72 0.63 0.7 0.61 0.68 0.85 0.83 0.71 0.63 0.6 0.57 0.52 0.63 0.7 0.55 0.66 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.1 0.15 0.08 0.25 0.23 0.3 0.24 0.35 0.38 0.34 0.14 0.13 0.16 0.22 0.05 0 0.06 0.08 0.09 0.17 0.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.29 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.12 0.15 0.14 0.16 0.11 0.07 0.05 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 2 0 0 1 1 1 0 2 2 0 0 0 1 0 0 1 4 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 1 2 1 4 4 8 2 5 8 11 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 3 8 7 17 8 13 21 8 7 8 15 27 41 4 4 9 3 3 0 4 1 0
Endoplasmic Reticulum 1 0 0 1 0 2 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 0 4 0 0 3 1 1 1 1 0 0 0 1 0 4 2 2 3 3 1 2 1 2 9
Golgi 0 1 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 3 1 0 5 1 0
Mitochondria 12 2 32 41 8 64 61 85 54 85 78 111 12 3 1 2 2 4 0 1 3 1 2 3
Nucleus 17 32 59 87 136 116 192 267 127 156 134 183 188 170 173 26 12 28 37 59 57 44 125 155
Nuclear Periphery 0 0 1 2 3 2 4 5 5 3 4 2 2 0 2 0 0 1 0 0 0 0 0 0
Nucleolus 2 3 7 18 12 47 59 110 48 79 84 93 32 26 38 9 1 1 4 7 7 13 37 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 3 1 0 1 1 2 1 2 0 0 0 2 3 1 0 1 6
Vac/Vac Membrane 6 18 2 3 5 6 5 11 10 3 2 2 3 4 6 5 3 7 11 10 5 4 11 20
Unique Cell Count 35 62 73 122 154 191 253 369 201 224 219 270 222 205 244 41 20 49 72 95 82 80 189 260
Labelled Cell Count 41 67 106 164 176 259 336 507 270 340 320 413 257 232 268 50 25 56 72 95 82 80 189 260


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.7 5.2 5.7 6.7 5.1 5.3 5.4 7.1 5.2 5.2 5.0 6.9 7.1 7.0 9.0 8.9 8.4 8.8 7.5 7.5
Std Deviation (1e-4) 3.2 1.5 2.1 2.9 3.2 2.1 2.0 2.2 5.4 2.1 2.0 1.7 1.5 2.0 2.2 2.3 2.5 2.8 4.2 1.7 2.1
Intensity Change (Log2) 0.11 0.36 -0.05 0.03 0.05 0.44 -0.0 -0.01 -0.06 0.39 0.44 0.42 0.79 0.76 0.68 0.74 0.51 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.3 0 0 1.6 0 0 0 0 2.1 2.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.4 -7.1 -1.6 -3.3 -3.7 -2.7 -0.9 -1.3 -0.4 -8.0 -9.4 -10.7 -4.4 -2.8 -4.2
Nucleus -1.5 1.5 -3.1 -0.9 -1.5 -2.8 -1.9 -3.1 -2.2 0.8 0.4 -1.7 -2.0 -1.9 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 -0.5 2.7 2.6 3.6 2.6 4.2 4.6 4.1 1.1 0.7 1.3 1.8 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7867 0.5384 0.7401 1.2074 1.646 0.4679 1.3802 0.076 0.1151 -0.0288 1.6504 0.3173 3.1309 1.6383 1.7121 2.2343 3.987 1.7724
Actin 0.076 0.0002 0.0323 0.0193 0.017 0.0001 0.0945 0.0021 0.0306 0.0035 0.1054 0.0029 0.0782 0.0001 0.0104 0.0476 0.197 0.0008
Bud 0.0008 0.0001 0.0011 0.0012 0.0008 0.0003 0.001 0.0016 0.0013 0.0002 0.0046 0.0003 0.0009 0.0002 0.0004 0.0017 0.0013 0.0001
Bud Neck 0.0014 0.0004 0.0054 0.1068 0.0004 0.0073 0.0054 0.0007 0.0012 0.0005 0.003 0.0024 0.0129 0.0003 0.0127 0.0006 0.0212 0.0036
Bud Periphery 0.0013 0 0.0011 0.0015 0.0019 0.0005 0.0019 0.0003 0.0015 0.0004 0.0093 0.0009 0.0017 0.0001 0.0007 0.0024 0.0035 0.0003
Bud Site 0.0026 0.0008 0.0119 0.0224 0.0018 0.0006 0.0036 0.0039 0.0144 0.0009 0.0138 0.0005 0.0083 0.0022 0.006 0.0072 0.0028 0.0002
Cell Periphery 0.0003 0 0.0003 0.0009 0.0004 0.0001 0.0005 0.0001 0.0002 0.0002 0.0005 0.0001 0.0009 0.0001 0.0002 0.0001 0.0007 0
Cytoplasm 0.0245 0.0149 0.0066 0.0026 0.006 0.0038 0.0232 0.0084 0.0027 0.0094 0.0095 0.0018 0.0053 0.016 0.0038 0.0036 0.0033 0.005
Cytoplasmic Foci 0.0303 0.0059 0.0448 0.0971 0.064 0.0003 0.0412 0.0039 0.0124 0.0064 0.0275 0.0042 0.0673 0.0004 0.0029 0.0068 0.0169 0.0003
Eisosomes 0.0006 0 0.0001 0.0003 0.0004 0 0.0008 0 0.0001 0.0012 0.001 0.0001 0.001 0 0.0001 0.0003 0.0016 0.0001
Endoplasmic Reticulum 0.0129 0.0009 0.0172 0.0005 0.0021 0.0002 0.0048 0.0003 0.002 0.0007 0.0034 0.001 0.0029 0.0005 0.0003 0.0005 0.0134 0.0004
Endosome 0.0397 0.0011 0.01 0.0544 0.0763 0.0009 0.0543 0.0012 0.0161 0.038 0.0209 0.0069 0.0393 0.0004 0.01 0.0086 0.0346 0.0011
Golgi 0.0116 0.0005 0.0047 0.0547 0.0292 0.0001 0.0301 0.0016 0.0153 0.0372 0.0216 0.004 0.0308 0 0.0019 0.0114 0.0145 0.0006
Lipid Particles 0.0167 0.0013 0.0087 0.0335 0.0734 0 0.0502 0.0004 0.0043 0.0394 0.0514 0.0205 0.0878 0 0.0038 0.0254 0.0353 0.0026
Mitochondria 0.0341 0.0004 0.0013 0.0111 0.2876 0.0003 0.0453 0.0007 0.0094 0.0471 0.158 0.0044 0.024 0.0004 0.0019 0.0392 0.0069 0.0057
None 0.0532 0.0389 0.0026 0.0005 0.0078 0.0003 0.0092 0.0095 0.0009 0.007 0.0027 0.0006 0.0045 0.0278 0.0014 0.054 0.0143 0.0147
Nuclear Periphery 0.0355 0.0082 0.0056 0.0011 0.0114 0.0035 0.0216 0.0106 0.011 0.0147 0.1305 0.0191 0.0086 0.0093 0.0026 0.0026 0.0854 0.0054
Nucleolus 0.0098 0.0221 0.0152 0.0038 0.0028 0.0633 0.0133 0.0198 0.0099 0.0146 0.0086 0.0515 0.0104 0.0196 0.0182 0.007 0.0296 0.0594
Nucleus 0.5859 0.8528 0.798 0.5504 0.2523 0.9082 0.5401 0.891 0.8311 0.7414 0.3193 0.8227 0.5325 0.8941 0.8964 0.7633 0.374 0.8724
Peroxisomes 0.0108 0.0051 0.0203 0.0302 0.0173 0 0.0237 0.001 0.0103 0.0071 0.0718 0.0028 0.0436 0 0.0015 0.0115 0.0583 0.0002
Punctate Nuclear 0.047 0.0454 0.0086 0.0052 0.1269 0.0082 0.0221 0.0423 0.0226 0.0258 0.0314 0.0499 0.0298 0.0277 0.0187 0.0052 0.0759 0.0264
Vacuole 0.0023 0.0009 0.0038 0.0017 0.0088 0.0018 0.0083 0.0004 0.0017 0.0013 0.0027 0.0016 0.0055 0.0006 0.0043 0.0005 0.0038 0.0005
Vacuole Periphery 0.0026 0.0002 0.0003 0.0007 0.0115 0.0002 0.0048 0.0001 0.001 0.003 0.0032 0.0019 0.0039 0.0001 0.0017 0.0005 0.0057 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5387 12.4542 12.9382 15.0199 25.2886 8.3545 16.3702 11.8987 13.753 17.184
Translational Efficiency 2.3256 1.4362 1.5712 0.9972 0.8799 3.8757 1.7056 2.0449 1.7509 1.683

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1407 905 878 167 971 1262 1106 1667 2378 2167 1984 1834

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.91 725.84 909.22 893.76 653.31 779.60 836.00 810.80 682.66 757.15 868.40 818.35
Standard Deviation 97.75 102.41 119.62 121.45 85.02 102.12 125.66 115.02 95.92 105.62 128.29 118.05
Intensity Change Log 2 0.046312 0.371289 0.346547 0.254966 0.355735 0.311578 0.150594 0.363818 0.329808

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000265 0.001100 0.001130 0.002126 0.000371 0.001245 0.001065 0.000949 0.000308 0.001185 0.001094 0.001056
Bud Neck 0.007866 0.013266 0.014683 0.006836 0.004882 0.009986 0.016173 0.016153 0.006648 0.011356 0.015514 0.015305
Bud Site 0.001698 0.004093 0.001671 0.007452 0.000853 0.002745 0.006491 0.006108 0.001353 0.003308 0.004358 0.006230
Cell Periphery 0.000148 0.000180 0.000078 0.000218 0.000163 0.000120 0.000166 0.000120 0.000154 0.000145 0.000127 0.000129
Cytoplasm 0.101444* 0.061936 0.006092 0.024514 0.055388 0.034665 0.028301 0.040095 0.082638 0.046054 0.018473 0.038676
Cytoplasmic Foci 0.016656 0.013073 0.000121 0.002132 0.015933 0.013186 0.000915 0.001664 0.016361 0.013139 0.000564 0.001707
Eisosomes 0.000048 0.000065 0.000034 0.000083 0.000057 0.000055 0.000037 0.000041 0.000052 0.000059 0.000036 0.000045
Endoplasmic Reticulum 0.002741 0.006907 0.006202 0.010280 0.003048 0.004264 0.004498 0.005316 0.002866 0.005367 0.005252 0.005768
Endosome 0.002750 0.003745 0.000410 0.002216 0.001914 0.003659 0.001161 0.001798 0.002409 0.003695 0.000829 0.001836
Golgi 0.001825 0.000962 0.000140 0.000843 0.000514 0.002117 0.001438 0.000717 0.001290 0.001634 0.000864 0.000728
Lipid Particles 0.004841 0.004676 0.000379 0.001367 0.007164 0.005901 0.000552 0.001049 0.005789 0.005390 0.000475 0.001078
Mitochondria 0.003312 0.006378 0.001489 0.009297 0.004378 0.015317 0.002318 0.002451 0.003748 0.011584 0.001951 0.003075
Mitotic Spindle 0.015015 0.009699 0.002816 0.037279 0.010001 0.018650 0.016873 0.020157 0.012968 0.014912 0.010652 0.021716
None 0.016744 0.015463 0.004033 0.028738 0.018413 0.007919 0.009195 0.009859 0.017426 0.011070 0.006910 0.011578
Nuclear Periphery 0.005669 0.004122 0.001138 0.001621 0.004907 0.005033 0.000984 0.001905 0.005358 0.004653 0.001052 0.001879
Nuclear Periphery Foci 0.002071 0.003581 0.000571 0.001216 0.002395 0.001913 0.000693 0.001729 0.002203 0.002610 0.000639 0.001682
Nucleolus 0.019157 0.031714 0.016472 0.032695 0.037674 0.036858 0.020413 0.023965 0.026718 0.034710 0.018669 0.024760
Nucleus 0.788002* 0.807153* 0.938905* 0.807926* 0.823953* 0.821857* 0.883831* 0.858220* 0.802682* 0.815716* 0.908203* 0.853640*
Peroxisomes 0.001801 0.003290 0.000313 0.003791 0.003023 0.004353 0.000504 0.000914 0.002300 0.003909 0.000419 0.001176
Vacuole 0.007520 0.007522 0.003155 0.018864 0.004499 0.008288 0.003968 0.006190 0.006286 0.007968 0.003608 0.007344
Vacuole Periphery 0.000424 0.001076 0.000167 0.000507 0.000470 0.001868 0.000424 0.000601 0.000443 0.001537 0.000310 0.000593

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.34 -15.24 -8.16 -4.51 -4.10 -1.42 -12.21 -11.30 -0.22 1.95 -2.69 -19.33 -15.25 -0.61 0.86
Bud Neck -3.58 -9.39 -2.91 0.80 5.50 -4.73 -12.04 -10.44 -5.80 1.94 -5.16 -14.50 -9.60 -5.05 2.48
Bud Site -3.31 -2.82 -3.72 -1.81 -3.07 -4.31 -5.76 -6.74 -2.86 0.58 -4.89 -5.92 -6.47 -2.85 -1.21
Cell Periphery -2.20 5.29 -1.45 0.12 -3.83 1.62 1.22 2.75 -0.12 1.54 0.38 3.22 2.65 1.14 -0.97
Cytoplasm 6.34 19.80 9.82 4.59 -3.64 3.78 8.13 4.84 1.40 -4.50 8.71 19.78 12.42 4.47 -7.91
Cytoplasmic Foci 1.57 12.23 10.80 6.68 -3.26 1.81 9.62 9.03 8.54 -1.93 2.39 15.42 13.87 10.64 -3.01
Eisosomes -3.50 4.21 -4.89 -3.10 -6.22 0.55 3.99 2.78 2.08 -1.39 -2.05 5.54 0.69 2.50 -4.15
Endoplasmic Reticulum -7.43 -9.79 -9.06 -4.97 -5.38 -2.77 -7.95 -10.10 -4.63 -1.87 -6.66 -12.06 -12.31 -3.77 -2.06
Endosome -0.61 8.64 1.93 1.83 -4.78 -2.14 3.73 1.70 2.97 -2.56 -1.64 8.48 4.19 3.98 -4.84
Golgi 0.91 2.08 1.53 1.37 -1.92 -2.44 -0.49 0.19 2.46 0.55 -0.77 1.09 1.48 2.65 0.17
Lipid Particles 0.05 10.83 5.96 4.95 -4.64 1.66 9.54 8.79 7.80 -4.16 0.84 14.15 12.13 10.05 -6.05
Mitochondria -2.25 3.20 -0.31 0.88 -0.93 -5.28 2.45 2.24 6.57 -0.72 -6.00 3.55 1.86 6.87 -2.11
Mitotic Spindle 1.69 3.12 -1.29 -1.87 -2.28 -2.71 -3.28 -3.95 -1.14 -0.09 -0.78 -0.80 -3.28 -2.67 -2.50
None 0.60 6.82 0.75 0.41 -2.61 4.99 5.86 5.13 0.26 -1.15 4.03 8.51 5.53 2.13 -3.55
Nuclear Periphery 3.59 9.01 6.08 2.67 -2.75 -0.03 4.90 3.18 4.58 -8.81 2.24 10.03 7.34 5.62 -8.50
Nuclear Periphery Foci -1.78 3.52 1.19 2.81 -2.23 1.00 3.64 0.46 -0.45 -3.12 -0.62 4.97 0.35 0.88 -3.48
Nucleolus -4.43 -1.31 0.35 3.22 1.10 0.18 3.85 3.48 3.67 -0.70 -3.89 1.39 -0.04 3.62 -1.34
Nucleus -2.11 -16.05 -2.23 -0.96 5.31 0.57 -5.37 -3.27 -4.14 2.79 -1.63 -14.32 -6.84 -5.15 7.06
Peroxisomes -3.04 6.13 0.35 2.31 -1.48 -1.62 5.13 4.65 6.43 -1.33 -3.71 7.52 5.47 7.68 -2.39
Vacuole 1.73 3.69 -3.39 -3.94 -4.50 -3.60 -1.30 -4.16 0.44 -2.70 -0.05 2.99 -1.44 -1.49 -4.84
Vacuole Periphery -2.33 4.42 0.13 2.35 -3.40 -3.04 1.56 0.21 2.98 -0.64 -3.74 3.24 0.02 3.40 -1.24