Standard name
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.11 0 0.07 0.05 0.09 0 0 0.1 0 0 0 0.07 0.13 0.17 0.1 0.2 0.18 0.05 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.06 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Mitochondria 0.34 0 0.44 0.34 0.05 0.34 0.24 0.23 0.27 0.38 0.36 0.41 0.05 0 0 0.05 0.1 0.08 0 0 0 0 0 0
Nucleus 0.49 0.52 0.81 0.71 0.88 0.61 0.76 0.72 0.63 0.7 0.61 0.68 0.85 0.83 0.71 0.63 0.6 0.57 0.52 0.63 0.7 0.55 0.66 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.1 0.15 0.08 0.25 0.23 0.3 0.24 0.35 0.38 0.34 0.14 0.13 0.16 0.22 0.05 0 0.06 0.08 0.09 0.17 0.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.29 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.12 0.15 0.14 0.16 0.11 0.07 0.05 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 2 0 0 1 1 1 0 2 2 0 0 0 1 0 0 1 4 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 1 2 1 4 4 8 2 5 8 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 3 8 7 17 8 13 21 8 7 8 15 27 41 4 4 9 3 3 0 4 1 0
Endoplasmic Reticulum 1 0 0 1 0 2 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 0 4 0 0 3 1 1 1 1 0 0 0 1 0 4 2 2 3 3 1 2 1 2 9
Golgi 0 1 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 3 1 0 5 1 0
Mitochondria 12 2 32 41 8 64 61 85 54 85 78 111 12 3 1 2 2 4 0 1 3 1 2 3
Nucleus 17 32 59 87 136 116 192 267 127 156 134 183 188 170 173 26 12 28 37 59 57 44 125 155
Nuclear Periphery 0 0 1 2 3 2 4 5 5 3 4 2 2 0 2 0 0 1 0 0 0 0 0 0
Nucleolus 2 3 7 18 12 47 59 110 48 79 84 93 32 26 38 9 1 1 4 7 7 13 37 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 3 1 0 1 1 2 1 2 0 0 0 2 3 1 0 1 6
Vac/Vac Membrane 6 18 2 3 5 6 5 11 10 3 2 2 3 4 6 5 3 7 11 10 5 4 11 20
Unique Cell Count 35 62 73 122 154 191 253 369 201 224 219 270 222 205 244 41 20 49 72 95 82 80 189 260
Labelled Cell Count 41 67 106 164 176 259 336 507 270 340 320 413 257 232 268 50 25 56 72 95 82 80 189 260


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.7 5.2 5.7 6.7 5.1 5.3 5.4 7.1 5.2 5.2 5.0 6.9 7.1 7.0 9.0 8.9 8.4 8.8 7.5 7.5
Std Deviation (1e-4) 3.2 1.5 2.1 2.9 3.2 2.1 2.0 2.2 5.4 2.1 2.0 1.7 1.5 2.0 2.2 2.3 2.5 2.8 4.2 1.7 2.1
Intensity Change (Log2) – – – 0.11 0.36 -0.05 0.03 0.05 0.44 -0.0 -0.01 -0.06 0.39 0.44 0.42 0.79 0.76 0.68 0.74 0.51 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.3 0 0 1.6 0 0 0 0 2.1 2.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.4 -7.1 -1.6 -3.3 -3.7 -2.7 -0.9 -1.3 -0.4 -8.0 -9.4 -10.7 -4.4 -2.8 -4.2
Nucleus -1.5 1.5 -3.1 -0.9 -1.5 -2.8 -1.9 -3.1 -2.2 0.8 0.4 -1.7 -2.0 -1.9 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 -0.5 2.7 2.6 3.6 2.6 4.2 4.6 4.1 1.1 0.7 1.3 1.8 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0