Standard name
Human Ortholog
Description (SGD) Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.96 0.94 0.88 0.74 0.54 0.52 0.48 0.48 0.47 0.44 0.97 0.98 0.97 0.95 0.97 0.97 0.96 0.92 0.9 0.8 0.8 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.26 0.38 0.31 0.47 0.46 0.52 0.4 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.1 0.11 0.13 0.19 0.2 0.16 0.14 0.23 0 0 0 0 0 0 0 0 0 0.05 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 1 1 0 1 0 0 0 0 1 1 0 0 0 1 2 1 2 3
Bud 0 0 0 0 1 4 11 8 13 8 4 11 0 0 0 0 0 0 0 0 0 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 2
Bud Site 0 0 0 0 0 0 0 0 2 1 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 6 6 3 12 7 9 5 6 7 13 1 1 2 7 8 4 0 0 2 0 0 1
Cytoplasm 181 280 292 315 315 423 371 333 265 255 235 262 95 173 201 178 150 223 182 263 274 133 269 298
Endoplasmic Reticulum 0 0 3 1 1 10 8 11 13 14 6 16 2 1 3 8 2 8 0 1 4 5 5 13
Endosome 0 0 3 3 7 7 12 23 7 10 8 23 0 1 1 0 0 0 0 3 6 0 3 5
Golgi 0 0 1 0 0 0 1 1 0 0 0 0 0 0 1 1 1 0 0 0 1 0 1 4
Mitochondria 0 2 1 11 28 152 259 200 262 245 259 238 0 0 1 2 0 4 0 2 2 8 15 19
Nucleus 5 1 1 1 1 1 9 20 9 11 6 4 3 1 0 2 1 1 0 4 0 0 1 1
Nuclear Periphery 0 0 0 0 2 1 6 5 1 5 3 6 0 0 0 0 1 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 1 1 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 3 3
Vac/Vac Membrane 3 2 4 18 37 62 91 124 110 84 70 135 1 3 3 3 3 6 3 6 1 8 20 16
Unique Cell Count 186 282 305 335 359 574 682 642 556 536 495 590 98 176 207 188 154 230 190 287 304 168 337 389
Labelled Cell Count 192 286 312 355 395 674 777 736 687 641 602 709 102 180 213 202 166 246 190 287 304 168 337 389


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 6.9 7.2 7.0 6.8 5.8 6.1 6.4 6.1 6.4 6.2 6.3 7.0 7.4 8.0 8.0 8.0 8.0 6.7 6.9 7.6
Std Deviation (1e-4) 1.2 1.1 1.3 1.7 1.3 1.3 1.5 1.4 1.4 1.5 1.5 1.3 1.0 1.2 1.0 1.4 1.4 1.5 1.4 1.1 1.2
Intensity Change (Log2) – – – -0.05 -0.09 -0.31 -0.26 -0.18 -0.25 -0.18 -0.22 -0.19 -0.06 0.04 0.15 0.15 0.14 0.14 -0.11 -0.06 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.2 0 0.1 0 0 0 0 0 0.2 0 0 0 0 0 0
Cytoplasm -1.0 -3.7 -8.0 -12.8 -13.3 -14.1 -14.1 -14.0 -15.0 0.5 1.5 0.8 -0.5 0.9 0.7
Endoplasmic Reticulum 0 0 0 0 0 1.4 1.6 0 1.7 0 0 0 0 0 0
Endosome 0 0 0 0 2.3 0 0 0 2.5 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 4.7 9.7 12.4 10.8 14.3 13.9 15.3 12.8 0 0 0 0 0 0
Nucleus 0 0 0 0 2.7 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 4.8 5.1 5.9 7.6 7.6 6.5 6.1 8.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5252 2.626 2.129 1.7484 1.8338 2.0153 2.9223 4.3194 3.7444 3.3799 2.9902 3.6475 1.8684 3.4815 2.9631 2.2635 1.7132 2.5524
Actin 0.0006 0.0004 0.0017 0.0005 0.0425 0.0005 0.0272 0.0029 0.0038 0.0116 0.0107 0.0014 0.0138 0.01 0.0464 0.0281 0.0005 0.0001
Bud 0.0001 0.0002 0.0002 0.0002 0.0003 0.0001 0.002 0.0009 0.0021 0.0027 0.0003 0.0025 0.0005 0.0008 0.0005 0.0008 0.0001 0
Bud Neck 0.0032 0.0002 0.0002 0.0003 0.0013 0.0008 0.005 0.0002 0.0005 0.0178 0.0007 0.0015 0.0029 0.0002 0.0002 0.0008 0.0003 0.0004
Bud Periphery 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0019 0.0005 0.0012 0.0016 0.0001 0.001 0.0008 0.0003 0.0003 0.0009 0 0
Bud Site 0.0005 0.001 0.0006 0.0002 0.0006 0.0001 0.004 0.0012 0.0072 0.0041 0.0003 0.0001 0.0136 0.0011 0.0005 0.0016 0.0001 0
Cell Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0.0003 0 0 0.0009 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.8261 0.9365 0.8664 0.8707 0.8495 0.8875 0.794 0.9031 0.8722 0.8163 0.8896 0.8908 0.7478 0.8903 0.9068 0.8285 0.985 0.9209
Cytoplasmic Foci 0.0173 0.0061 0.0097 0.008 0.0149 0.0141 0.0218 0.0123 0.0139 0.0381 0.0201 0.0214 0.023 0.0066 0.008 0.0362 0.0036 0.0078
Eisosomes 0 0 0 0 0.0002 0 0.0002 0 0 0.0001 0 0 0.0006 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0065 0.0029 0.0042 0.003 0.002 0.0038 0.0053 0.0009 0.0025 0.0016 0.0035 0.0019 0.0181 0.0053 0.0024 0.0014 0.0016 0.0029
Endosome 0.0184 0.0063 0.0073 0.0049 0.0063 0.0148 0.0129 0.0058 0.0054 0.008 0.0392 0.0108 0.0299 0.0038 0.0025 0.0129 0.0015 0.0031
Golgi 0.0037 0.0003 0.001 0.0003 0.0041 0.0015 0.0035 0.0003 0.0007 0.0022 0.005 0.0011 0.0085 0.001 0.0035 0.0133 0.0003 0.0003
Lipid Particles 0.0003 0.0001 0.0001 0.0002 0.0011 0.0001 0.0047 0.0001 0.0003 0.0014 0.0035 0.0032 0.0058 0.0001 0.0001 0.0097 0 0
Mitochondria 0.0009 0.0002 0.0008 0.0003 0.0011 0.0011 0.0021 0.0003 0.0005 0.0006 0.0018 0.0023 0.0052 0.0002 0.0021 0.0125 0.0001 0.0001
None 0.1119 0.039 0.0998 0.1049 0.0657 0.0672 0.0989 0.0657 0.0768 0.0622 0.0086 0.047 0.0996 0.0766 0.0233 0.0047 0.0034 0.0617
Nuclear Periphery 0.0025 0.0015 0.0014 0.0013 0.0008 0.0013 0.0019 0.0005 0.0018 0.0027 0.0068 0.0015 0.0051 0.0011 0.0005 0.0006 0.0007 0.0005
Nucleolus 0.0001 0 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0005 0.0001 0.0001 0.0006 0 0 0.0001 0 0
Nucleus 0.0034 0.0027 0.0039 0.0027 0.0026 0.0044 0.0037 0.0028 0.0049 0.0064 0.0037 0.0053 0.0024 0.0012 0.0013 0.0017 0.0022 0.0013
Peroxisomes 0.001 0.0001 0.0002 0.0002 0.0049 0.0002 0.0059 0.0006 0.0013 0.0129 0.0019 0.0012 0.0048 0.0003 0.0007 0.0444 0 0.0001
Punctate Nuclear 0.0009 0.0004 0.0009 0.0008 0.0006 0.0008 0.0021 0.0012 0.0031 0.0073 0.0024 0.0059 0.0008 0.0003 0.0002 0.0005 0.0002 0.0002
Vacuole 0.0021 0.0016 0.0011 0.0011 0.0009 0.0013 0.0019 0.0006 0.001 0.0012 0.0008 0.0008 0.0134 0.0006 0.0005 0.0008 0.0003 0.0003
Vacuole Periphery 0.0004 0.0003 0.0003 0.0002 0.0002 0.0004 0.0004 0.0001 0.0002 0.0003 0.0006 0.0003 0.002 0.0001 0.0001 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4683 13.0595 13.681 14.0899 12.256 17.1775 16.5056 12.8439 12.7247 15.8992
Translational Efficiency 1.0888 1.2255 1.0063 0.7913 1.016 0.882 0.9947 1.0976 1.0816 0.8137

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1844 1875 3391 98 2290 1700 2163 2623 4134 3575 5554 2721

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 764.20 887.41 1002.52 1075.18 843.61 848.20 1026.54 1037.46 808.19 868.76 1011.87 1038.82
Standard Deviation 106.73 132.95 125.38 148.04 106.20 119.76 131.19 135.64 113.52 128.35 128.21 136.29
Intensity Change Log 2 — 0.215651 0.391609 0.492556 — 0.007828 0.283142 0.298408 — 0.110346 0.335715 0.393950

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000164 0.000466 0.000057 0.006282 0.000217 0.000986 0.000063 0.000336 0.000194 0.000713 0.000059 0.000550
Bud Neck 0.009569 0.017794 0.003229 0.002586 0.012548 0.027464 0.002630 0.007182 0.011219 0.022392 0.002996 0.007016
Bud Site 0.008297 0.025916 0.003931 0.040975 0.010010 0.049768 0.004608 0.021494 0.009246 0.037258 0.004194 0.022196
Cell Periphery 0.000105 0.000067 0.000036 0.000266 0.000063 0.000161 0.000033 0.000057 0.000082 0.000112 0.000035 0.000065
Cytoplasm 0.584718* 0.583237* 0.703904* 0.462988* 0.579263* 0.469106* 0.713664* 0.714524* 0.581696* 0.528965* 0.707705* 0.705465*
Cytoplasmic Foci 0.184822* 0.154651 0.003979 0.048777 0.192938* 0.232465* 0.005188 0.029556 0.189318* 0.191653* 0.004449 0.030248
Eisosomes 0.000059 0.000032 0.000005 0.000084 0.000044 0.000071 0.000007 0.000017 0.000050 0.000050 0.000006 0.000019
Endoplasmic Reticulum 0.001102 0.001305 0.000348 0.003836 0.001288 0.000876 0.000415 0.000453 0.001205 0.001101 0.000374 0.000575
Endosome 0.015776 0.016097 0.000394 0.004564 0.014450 0.033523 0.000322 0.002523 0.015041 0.024384 0.000366 0.002596
Golgi 0.004284 0.006524 0.000045 0.000771 0.004729 0.013333 0.000041 0.001822 0.004530 0.009762 0.000044 0.001784
Lipid Particles 0.005145 0.001866 0.000182 0.002181 0.003186 0.003147 0.000237 0.000537 0.004059 0.002475 0.000204 0.000596
Mitochondria 0.003195 0.006342 0.000572 0.000204 0.003199 0.012662 0.000367 0.001042 0.003197 0.009347 0.000492 0.001012
Mitotic Spindle 0.002424 0.008253 0.002542 0.003088 0.003360 0.014675 0.004293 0.021149 0.002943 0.011307 0.003224 0.020499
None 0.005079 0.002559 0.006181 0.001897 0.002490 0.002018 0.007239 0.003158 0.003645 0.002302 0.006593 0.003113
Nuclear Periphery 0.000295 0.000293 0.000614 0.000637 0.000258 0.000174 0.000565 0.000465 0.000274 0.000236 0.000595 0.000472
Nuclear Periphery Foci 0.001339 0.000955 0.000199 0.009377 0.000671 0.000710 0.000467 0.000437 0.000969 0.000838 0.000303 0.000759
Nucleolus 0.001483 0.001165 0.000136 0.000158 0.000882 0.001167 0.000114 0.000425 0.001150 0.001166 0.000127 0.000416
Nucleus 0.093128 0.068964 0.248560* 0.101639 0.086954 0.033226 0.229237* 0.097677 0.089708 0.051970 0.241035* 0.097820
Peroxisomes 0.004025 0.006833 0.000067 0.002232 0.004765 0.016353 0.000073 0.002643 0.004435 0.011360 0.000069 0.002628
Vacuole 0.073019 0.092581 0.024934 0.307268* 0.076763 0.084398 0.030389 0.093889 0.075093 0.088690 0.027058 0.101574
Vacuole Periphery 0.001972 0.004101 0.000087 0.000190 0.001926 0.003718 0.000050 0.000614 0.001946 0.003919 0.000072 0.000598

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.26 5.14 -1.88 -1.63 -1.95 -7.30 5.73 -2.58 6.45 -7.04 -8.30 8.08 -3.44 4.18 -5.54
Bud Neck -5.03 6.50 7.42 11.17 2.07 -7.53 9.76 5.97 11.73 -5.95 -8.72 11.67 6.24 13.67 -6.23
Bud Site -10.22 5.40 -1.86 1.39 -2.85 -16.15 6.27 -3.09 13.52 -7.55 -18.84 8.81 -4.06 12.80 -9.06
Cell Periphery 3.62 6.01 -3.10 -4.10 -4.81 -3.88 9.23 4.02 4.34 -4.45 -2.27 7.68 4.39 4.31 -4.96
Cytoplasm 0.24 -6.57 8.75 8.65 10.74 11.45 -5.43 -6.48 -17.49 -1.21 7.83 -8.92 -5.61 -12.43 1.64
Cytoplasmic Foci 5.24 40.22 24.07 19.92 -5.03 -5.81 43.81 37.30 38.19 -17.24 -0.25 59.56 48.79 45.79 -19.10
Eisosomes 10.91 23.95 -2.31 -4.75 -6.97 -8.32 20.53 13.71 16.74 -12.63 0.06 32.05 18.20 15.49 -17.90
Endoplasmic Reticulum -1.32 6.10 -5.09 -4.66 -6.13 2.77 4.46 5.49 3.32 3.14 0.93 7.24 5.72 4.55 -2.58
Endosome 0.09 17.57 12.68 12.01 -6.85 -9.38 16.37 14.43 17.33 -10.45 -7.63 23.81 20.79 21.99 -11.02
Golgi -2.40 7.77 7.12 9.23 -2.00 -7.45 9.63 6.33 11.29 -2.43 -7.24 12.36 7.30 12.66 -2.47
Lipid Particles 6.20 9.73 5.15 -1.60 -8.95 0.01 10.11 8.96 6.03 -9.80 4.60 13.77 12.05 7.91 -15.88
Mitochondria -3.55 5.67 6.57 8.52 3.89 -5.85 5.66 4.77 7.71 -4.26 -6.82 7.82 6.88 10.31 -2.72
Mitotic Spindle -4.17 -0.70 -0.99 1.41 -0.77 -5.81 -1.93 -6.76 -0.34 -5.22 -7.03 -1.60 -7.41 -2.04 -6.81
None 3.30 -1.67 4.26 1.55 10.76 0.85 -9.26 -1.61 -3.61 14.89 2.93 -7.10 1.03 -3.19 17.11
Nuclear Periphery -0.11 -20.62 -6.99 -6.93 -1.07 3.39 -22.77 -16.35 -17.99 5.42 1.64 -30.89 -17.10 -17.50 5.96
Nuclear Periphery Foci 1.63 5.93 -5.71 -6.07 -6.75 -0.25 -0.36 1.70 1.08 2.07 0.94 5.96 1.47 0.32 -5.82
Nucleolus 2.06 9.12 9.34 10.33 1.92 -1.02 7.36 3.89 3.35 -6.78 0.26 11.64 7.37 6.03 -6.46
Nucleus 6.32 -45.81 -2.43 -4.10 12.28 19.94 -40.00 -13.24 -32.83 29.36 16.36 -62.10 -13.82 -26.91 41.79
Peroxisomes -3.47 7.42 5.30 9.51 -2.01 -10.60 9.31 5.04 14.36 -5.44 -10.34 11.90 5.75 15.39 -5.57
Vacuole -5.61 8.22 -12.34 -11.58 -13.19 -2.44 2.55 -18.17 -15.23 -19.45 -5.75 8.63 -21.61 -17.39 -26.54
Vacuole Periphery -5.07 12.46 11.95 10.45 -2.95 -4.53 12.34 8.20 8.85 -2.67 -6.81 17.38 10.28 12.47 -2.57
Standard name
Human Ortholog
Description (SGD) Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fol3

Fol3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)