Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0.15 0 – 0 0.08 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.06 0.05 0.06 0.05 – 0.1 0.07 0.07 0.05 0.07 0.11 0.17 0.07 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.2 0 – 0.17 0 0 0.05 – 0.06 0 0.08 0 0.14 0.12 0.14 0.31 0.34 0.3 0 0 0 0 0 0.06
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.11 – 0 0 0 0 – 0.06 0 0.08 0.06 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Nucleus 0 0 – 0.22 0 0.07 0 – 0.07 0.1 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.66 0.63 – 0.56 0.4 0.59 0.42 – 0.41 0.51 0.43 0.42 0.6 0.6 0.58 0.55 0.54 0.51 0 0.05 0 0 0.06 0
Peroxisomes 0.08 0 – 0 0 0 0.07 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.72 0.67 – 0.61 0.86 0.74 0.79 – 0.67 0.73 0.6 0.7 0.73 0.69 0.61 0.63 0.62 0.61 0.87 0.8 0.84 0.81 0.79 0.76
Vac/Vac Membrane 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Bud 0 5 – 0 0 3 1 – 1 1 2 4 0 1 0 1 0 0 1 2 0 3 6 8
Bud Neck 0 2 – 0 3 2 4 – 3 2 4 3 0 1 1 0 0 2 0 1 0 1 2 2
Bud Site 2 4 – 0 1 8 1 – 2 10 6 9 0 1 1 1 1 5 – – – – – –
Cell Periphery 0 1 – 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 – 1 6 3 7 – 8 9 7 7 16 40 68 8 11 6 1 1 0 0 0 3
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 1 1 1 3 3 0 0 0 0 0 2
Endosome 17 8 – 3 4 2 7 – 5 2 8 2 30 44 55 38 37 42 3 9 0 8 14 14
Golgi 0 0 – 0 0 0 0 – 1 0 0 0 0 0 0 0 2 0 0 1 0 1 0 2
Mitochondria 4 22 – 0 1 1 1 – 5 5 8 8 2 5 2 5 9 10 0 2 0 0 2 5
Nucleus 1 8 – 4 5 4 6 – 6 12 6 7 9 6 12 0 2 3 0 1 0 5 8 3
Nuclear Periphery 0 1 – 1 0 0 0 – 0 0 1 0 1 0 2 0 0 0 0 0 0 0 0 1
Nucleolus 57 128 – 10 46 32 62 – 33 64 43 55 132 217 234 66 59 73 2 11 0 8 18 7
Peroxisomes 7 7 – 0 1 0 10 – 3 0 5 2 9 14 15 1 3 3 0 3 0 1 2 1
SpindlePole 63 136 – 11 99 40 116 – 54 92 61 92 161 248 245 76 68 86 89 173 17 186 260 204
Vac/Vac Membrane 0 6 – 1 2 1 1 – 2 0 1 0 1 3 5 11 7 18 0 2 0 4 3 5
Unique Cell Count 87 204 18 115 54 146 81 126 101 132 220 361 404 121 109 142 103 217 21 229 329 270
Labelled Cell Count 153 334 32 168 96 218 123 197 152 189 361 581 641 209 202 252 103 217 21 229 329 270


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.4 – 9.3 8.1 5.2 7.0 – 7.1 6.0 8.1 6.0 7.1 6.9 7.4 9.1 9.9 9.1 6.3 6.4 6.6
Std Deviation (1e-4) 1.1 1.6 – 2.9 2.1 1.4 1.7 – 3.1 1.5 3.4 1.6 1.7 1.7 1.8 2.1 2.0 1.7 1.4 1.4 1.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6432 1.8993 2.0574 2.2959 1.2985 1.6184 3.3424 2.6338 3.3327 3.2141 2.7623 2.9042 4.8698 5.6725 5.177 5.6425 4.4817 4.6334
Actin 0.0051 0 0 0 0 0.0001 0.036 0 0.0007 0.0272 0.0163 0.0037 0.0089 0 0.0072 0 0.0008 0.001
Bud 0 0 0 0 0 0.0001 0.0004 0 0.0001 0.0005 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0004 0.0003 0.0001 0.0019 0.0001 0.0002 0.0009 0.0009 0.0002 0.0009 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0008 0.0001
Bud Periphery 0.0001 0 0 0 0 0 0.0007 0 0.0001 0.0011 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Site 0.0002 0.0001 0 0.0003 0 0.0001 0.0023 0.0011 0.0005 0.0011 0.0003 0.0001 0.0004 0.0001 0.0003 0 0.0003 0
Cell Periphery 0 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.0003 0 0.0001 0.0001 0 0.0001 0.0041 0.0002 0.0003 0.0001 0.0004 0 0.0013 0.0001 0.0009 0 0.004 0.0001
Cytoplasmic Foci 0.0127 0.0043 0.0034 0.024 0.0189 0.0034 0.034 0.0093 0.0083 0.0092 0.0362 0.0103 0.007 0.002 0.0064 0.0088 0.0207 0.0019
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0007 0 0 0 0 0 0.0109 0 0.0001 0.0003 0.0006 0.0004 0.001 0 0.0004 0 0.0005 0.0002
Endosome 0.0025 0 0.0001 0.0013 0.0006 0.0001 0.0173 0.0002 0.0045 0.0034 0.0031 0.0021 0.0055 0 0.0027 0.0001 0.0058 0.0028
Golgi 0.0012 0.0001 0.0001 0.0006 0.0007 0.0001 0.0062 0.0002 0.0031 0.0058 0.0051 0.0084 0.0018 0.0001 0.0005 0.0002 0.0011 0.0009
Lipid Particles 0.0065 0.0004 0.002 0.0011 0.0192 0.0024 0.0468 0.0008 0.007 0.0041 0.0469 0.0194 0.0059 0.0003 0.0022 0.001 0.0183 0.0033
Mitochondria 0.0012 0 0 0 0 0.0002 0.0034 0 0.0036 0.006 0.0017 0.0015 0.0009 0 0.0004 0 0.0007 0.002
None 0.0018 0 0 0 0 0.0001 0.0032 0 0.0002 0.0001 0.001 0 0.0034 0 0.001 0 0.043 0.0002
Nuclear Periphery 0.0018 0 0.0004 0.0002 0.0002 0.0002 0.0242 0.0004 0.0032 0.002 0.003 0.0004 0.0113 0.0008 0.0009 0.0005 0.0068 0.0057
Nucleolus 0.0261 0.0513 0.0242 0.0018 0.002 0.0126 0.019 0.0862 0.0475 0.0022 0.0067 0.005 0.0029 0.0566 0.0246 0.0012 0.0127 0.0095
Nucleus 0.0054 0.0062 0.0075 0.0006 0.0003 0.0026 0.0095 0.0912 0.0161 0.0014 0.0009 0.0009 0.0017 0.0208 0.0037 0.0005 0.0093 0.0029
Peroxisomes 0.0007 0.0001 0.0002 0.0004 0.0007 0.0001 0.0279 0.0004 0.0062 0.0011 0.0259 0.0007 0.0019 0 0.0005 0.0002 0.0012 0.0008
Punctate Nuclear 0.9326 0.9371 0.9617 0.9677 0.9572 0.9777 0.75 0.809 0.8972 0.9324 0.8508 0.9465 0.9446 0.9189 0.9475 0.9873 0.873 0.9679
Vacuole 0.0003 0 0 0 0 0 0.0014 0.0001 0.0005 0.0003 0.0001 0 0.0003 0 0.0001 0 0.0005 0.0002
Vacuole Periphery 0.0003 0 0 0 0 0 0.0015 0 0.0004 0.0003 0.0003 0.0002 0.0005 0 0.0001 0 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5935 6.1456 4.5603 8.8786 7.7993 10.3065 11.2626 3.1455 8.2095 13.7062
Translational Efficiency 2.7769 2.9085 1.9411 1.4234 3.0147 2.4007 1.6788 4.5571 1.5714 1.6048

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
192 622 1576 1499 1735 2123 214 1789 1927 2745 1790 3288

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.99 857.80 1022.01 1011.32 786.31 872.68 1089.70 1088.20 789.96 869.31 1030.10 1053.15
Standard Deviation 111.22 135.75 134.91 150.04 94.62 112.61 132.58 144.67 97.02 118.42 136.42 152.04
Intensity Change Log 2 — 0.059766 0.312463 0.297293 — 0.150355 0.470761 0.468774 — 0.104739 0.391978 0.383626

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000711 0.001473 0.000074 0.000276 0.000479 0.000663 0.000152 0.000157 0.000502 0.000847 0.000083 0.000211
Bud Neck 0.005310 0.004887 0.001117 0.002336 0.001974 0.004136 0.001446 0.001190 0.002307 0.004306 0.001157 0.001712
Bud Site 0.009051 0.008821 0.002254 0.008175 0.001715 0.003657 0.001885 0.005764 0.002445 0.004828 0.002210 0.006863
Cell Periphery 0.000273 0.000125 0.000025 0.000066 0.000073 0.000086 0.000035 0.000023 0.000093 0.000095 0.000026 0.000043
Cytoplasm 0.017661 0.008428 0.001055 0.017983 0.002265 0.005002 0.000821 0.004099 0.003799 0.005778 0.001027 0.010428
Cytoplasmic Foci 0.019602 0.030124 0.002630 0.012398 0.010918 0.021780 0.003926 0.006910 0.011783 0.023671 0.002785 0.009412
Eisosomes 0.000074 0.000070 0.000009 0.000009 0.000092 0.000058 0.000020 0.000005 0.000090 0.000061 0.000010 0.000007
Endoplasmic Reticulum 0.002856 0.001639 0.000231 0.000397 0.001324 0.001056 0.000497 0.000112 0.001476 0.001188 0.000263 0.000242
Endosome 0.003809 0.006716 0.000346 0.004065 0.002392 0.006722 0.000876 0.002408 0.002534 0.006721 0.000409 0.003164
Golgi 0.004149 0.006830 0.000331 0.002316 0.004122 0.005451 0.000842 0.001049 0.004125 0.005763 0.000392 0.001627
Lipid Particles 0.002877 0.004339 0.000208 0.001594 0.003234 0.006321 0.000369 0.000416 0.003199 0.005872 0.000227 0.000953
Mitochondria 0.001197 0.006740 0.000069 0.000915 0.000866 0.002001 0.000080 0.000512 0.000899 0.003075 0.000070 0.000696
Mitotic Spindle 0.711769* 0.773115* 0.970217* 0.918514* 0.798194* 0.774164* 0.945726* 0.966632* 0.789583* 0.773927* 0.967289* 0.944695*
None 0.003056 0.002114 0.000282 0.002625 0.001563 0.002519 0.000238 0.000197 0.001712 0.002427 0.000277 0.001304
Nuclear Periphery 0.000668 0.000389 0.000119 0.000630 0.000318 0.000589 0.000194 0.000135 0.000353 0.000544 0.000128 0.000361
Nuclear Periphery Foci 0.058773 0.062823 0.003881 0.004431 0.064682 0.046120 0.011963 0.001722 0.064093 0.049904 0.004847 0.002957
Nucleolus 0.086683 0.039773 0.007800 0.005584 0.069576 0.073159 0.011888 0.001806 0.071281 0.065594 0.008288 0.003529
Nucleus 0.055451 0.022013 0.006749 0.009189 0.020704 0.033026 0.011492 0.001428 0.024166 0.030530 0.007316 0.004966
Peroxisomes 0.010662 0.015442 0.002046 0.002651 0.014914 0.011998 0.005195 0.002157 0.014490 0.012779 0.002422 0.002382
Vacuole 0.004872 0.003621 0.000518 0.004628 0.000507 0.001134 0.002292 0.002642 0.000942 0.001697 0.000730 0.003548
Vacuole Periphery 0.000496 0.000519 0.000040 0.001215 0.000086 0.000356 0.000063 0.000636 0.000127 0.000393 0.000042 0.000900

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.93 1.64 0.88 1.29 -2.37 -0.88 -2.04 0.78 2.00 3.23 -1.46 -0.11 0.16 1.88 0.67
Bud Neck 1.19 2.65 1.31 0.14 -3.89 -5.26 -4.34 -3.36 1.29 3.15 -4.61 -2.78 -4.72 -0.21 -1.23
Bud Site 0.46 2.42 -1.08 -2.53 -7.45 -6.22 -5.43 -7.66 -5.09 -0.12 -5.29 -3.19 -10.77 -7.15 -7.17
Cell Periphery 1.12 2.40 1.94 3.52 -3.31 -0.50 -1.50 5.96 7.02 4.60 -0.27 3.35 3.65 5.85 -0.29
Cytoplasm 1.09 2.57 -1.88 -4.62 -8.08 -1.72 -0.38 -3.71 -2.34 -1.83 -1.40 2.00 -7.08 -6.21 -9.00
Cytoplasmic Foci -1.07 4.58 2.10 4.54 -6.81 -4.52 0.53 2.15 6.43 0.73 -5.34 7.16 1.45 7.03 -6.69
Eisosomes 0.50 8.00 8.72 11.65 2.73 8.46 9.77 23.37 23.32 5.96 8.31 20.40 24.02 25.34 6.94
Endoplasmic Reticulum 1.38 3.88 4.12 6.53 0.90 5.85 0.24 17.55 13.76 3.77 4.62 7.47 14.58 12.20 4.52
Endosome -1.83 4.80 0.83 2.40 -4.73 -3.92 1.40 1.84 4.75 0.07 -4.53 7.39 1.01 4.90 -4.58
Golgi -3.17 6.87 4.23 6.93 -1.98 1.43 9.58 15.54 10.87 3.33 0.16 17.51 13.45 11.49 -1.42
Lipid Particles -2.37 3.56 1.87 4.26 -2.36 -1.04 5.39 7.31 5.42 2.52 -1.60 7.74 6.25 6.06 -1.99
Mitochondria -1.64 1.60 0.37 1.77 -3.01 -1.85 0.54 0.22 2.19 -0.39 -2.46 1.84 -0.23 2.46 -3.33
Mitotic Spindle -2.28 -9.80 -7.86 -9.28 5.64 -1.74 -5.47 -22.77 -23.00* -5.22 -2.14 -19.89 -21.34 -22.18 0.32
None 0.33 2.55 0.15 -0.22 -3.41 -1.16 5.81 7.17 7.33 1.00 -1.02 6.94 2.17 3.02 -3.09
Nuclear Periphery 2.11 5.63 -0.60 -2.53 -5.63 -3.94 -3.21 0.84 3.59 3.32 -3.40 1.80 -2.91 -0.07 -3.80
Nuclear Periphery Foci 0.06 5.92 6.36 10.41 2.25 5.95 9.39 21.31* 18.51* 4.08 5.43 18.50 21.86 20.82 5.02
Nucleolus 3.77 5.95 6.97 7.77 4.60 1.17 7.62 20.17 20.87 5.05 3.06 14.88 20.65 21.49 7.91
Nucleus 2.65 3.04 2.41 -0.71 -1.78 -2.50 -1.60 6.46 8.33 3.16 -1.02 0.43 2.97 4.19 2.08
Peroxisomes -2.13 5.98 5.73 8.79 -0.61 2.88 2.61 12.57 11.28 4.92 1.75 11.29 13.00 13.74 2.54
Vacuole 0.37 0.64 -1.77 -3.67 -4.38 -2.30 -2.51 -4.91 -4.62 1.03 -1.55 -3.42 -7.44 -6.91 -3.34
Vacuole Periphery 0.08 2.36 -1.19 -1.22 -2.51 -2.14 -4.21 -2.04 -1.17 -1.27 -2.42 -0.60 -3.10 -1.97 -3.03
Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
Localization
Cell Percentages spindle pole (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc24

Spc24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available