Standard name
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.06 0.07 0.14 0.21 0.06 0.15 0.13 0.06 0.13 0.11 0.14 0.13 0.22 0.26 0.11 0.05 0 0 0.06 0.12 0.11 0.11
Endoplasmic Reticulum 0 0 0 0.15 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0.17 0 0 0
Endosome 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0 0 0.08 0.15 0.35 0.23 0.61 0.38 0 0 0 0 0.14 0.08 0.07 0 0.13 0.13 0.13
Nucleus 0 0.1 0.05 0.16 0.19 0.29 0.32 0.39 0.36 0.28 0.34 0.14 0.14 0.17 0 0 0 0 0 0 0.1 0.08
Nuclear Periphery 0.29 0.14 0 0 0.14 0.19 0.12 0.21 0.18 0.15 0.1 0.24 0.26 0.18 0.11 0.14 0.72 0.72 0.55 0.44 0.26 0.21
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.82 0.77 0.58 0.69 0.49 0.41 0.22 0.33 0.25 0.29 0.62 0.49 0.53 0.81 0.9 0.06 0.12 0.11 0.13 0.22 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 1 0 3 2 0 0 0 0 0 0 0 0 0 4 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 2 3 2 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 11 3 15 9 27 39 7 23 27 45 12 43 63 4 1 3 2 2 8 27 33
Endoplasmic Reticulum 7 5 0 11 0 0 0 3 4 5 1 5 5 5 1 0 9 5 7 2 10 12
Endosome 1 1 1 0 5 4 11 0 3 0 4 0 2 4 1 1 3 0 0 0 7 8
Golgi 1 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0 2 0
Mitochondria 55 4 0 3 6 15 44 40 41 157 125 0 0 1 1 3 14 10 1 10 31 39
Nucleus 6 14 1 12 29 52 98 45 65 73 112 13 27 41 1 0 1 3 0 3 24 25
Nuclear Periphery 46 21 0 1 22 34 36 24 32 38 33 22 50 45 4 3 135 119 25 33 62 64
Nucleolus 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 0 3 0 3 2 0 0 4 1 0 1 9 5
Vac/Vac Membrane 101 121 17 42 108 88 124 25 60 65 97 58 95 129 29 19 11 19 5 9 52 99
Unique Cell Count 160 147 22 73 156 178 302 114 180 257 333 93 192 245 36 21 189 166 46 76 241 301
Labelled Cell Count 226 177 22 84 181 220 354 146 233 371 424 110 227 292 41 28 189 166 46 76 241 301


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.6 5.3 5.8 6.2 5.4 5.5 4.1 4.5 4.1 4.4 6.2 6.0 5.9 6.8 6.7 5.5 5.8
Std Deviation (1e-4) 0.4 1.0 0.6 1.5 1.6 1.5 2.2 0.9 0.6 1.1 1.2 0.9 1.1 1.2 1.0 1.6 1.1 1.0
Intensity Change (Log2) 0.12 0.23 0.02 0.04 -0.38 -0.24 -0.39 -0.28 0.22 0.17 0.14 0.35 0.34 0.06 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 1.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 2.5 2.7 3.2 3.0 2.4 2.8 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2.7 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 -0.8 -2.5 -3.3 -5.1 -4.0 -5.1 -4.7 -1.3 -2.5 -2.2 0.3 1.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available