Standard name | |
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Description | Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 2 | 5 | 3 | 3 | 3 | 7 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 0 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 8 | 3 | 5 | 5 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 6 | 8 | 7 | 8 | 17 | 28 | 66 | 36 | 42 | 35 | 46 | 22 | 57 | 102 | 42 | 35 | 19 | 2 | 0 | 0 | 1 | 2 | 3 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 10 | 9 | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 2 | 3 | 8 | 37 | 48 | 7 | 42 | 22 | 11 | 28 | 24 | 35 | 43 | 80 | 109 | 39 | 24 | 16 | 6 | 10 | 8 | 25 | 54 | 62 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 1 | 3 | 0 |
Mitochondria | 9 | 11 | 0 | 16 | 2 | 70 | 21 | 60 | 86 | 72 | 103 | 89 | 5 | 3 | 2 | 18 | 16 | 19 | 0 | 7 | 3 | 5 | 7 | 7 |
Nucleus | 235 | 318 | 111 | 71 | 56 | 30 | 48 | 71 | 27 | 54 | 64 | 47 | 78 | 56 | 58 | 144 | 101 | 96 | 178 | 259 | 68 | 53 | 48 | 51 |
Nuclear Periphery | 8 | 6 | 3 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 2 | 1 | 6 | 2 | 4 | 3 | 5 | 7 |
Nucleolus | 47 | 54 | 42 | 156 | 62 | 32 | 22 | 19 | 16 | 19 | 22 | 16 | 164 | 194 | 89 | 106 | 87 | 116 | 113 | 111 | 82 | 75 | 76 | 73 |
Peroxisomes | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 1 | 2 | 1 | 2 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 4 | 11 | 100 | 56 | 16 | 32 | 34 | 13 | 32 | 21 | 40 | 51 | 89 | 120 | 37 | 24 | 26 | 4 | 5 | 11 | 26 | 42 | 52 |
Vac/Vac Membrane | 13 | 34 | 4 | 4 | 15 | 7 | 20 | 26 | 26 | 24 | 19 | 36 | 4 | 9 | 14 | 25 | 31 | 20 | 22 | 39 | 8 | 36 | 44 | 43 |
Unique Cell Count | 292 | 399 | 163 | 244 | 175 | 138 | 163 | 248 | 166 | 207 | 224 | 243 | 268 | 338 | 350 | 335 | 260 | 252 | 344 | 446 | 191 | 237 | 298 | 314 |
Labelled Cell Count | 319 | 438 | 187 | 399 | 253 | 193 | 220 | 312 | 226 | 284 | 299 | 317 | 370 | 493 | 498 | 424 | 332 | 318 | 344 | 446 | 191 | 237 | 298 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 7.5 | 7.9 | 7.4 | 7.5 | 4.4 | 5.3 | 5.0 | 4.5 | 4.6 | 4.6 | 4.7 | 8.0 | 6.7 | 6.1 | 8.6 | 9.9 | 8.7 | 7.2 | 6.8 | 7.0 |
Std Deviation (1e-4) | 1.4 | 1.4 | 1.4 | 1.4 | 5.1 | 1.7 | 1.7 | 1.5 | 1.4 | 1.3 | 1.0 | 1.2 | 1.9 | 1.5 | 1.7 | 2.0 | 2.0 | 2.2 | 1.4 | 1.5 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.08 | -0.83 | -0.57 | -0.67 | -0.82 | -0.76 | -0.78 | -0.75 | 0.02 | -0.25 | -0.37 | 0.13 | 0.32 | 0.15 | -0.13 | -0.22 | -0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.1 | -0.1 | 2.3 | 3.5 | 5.6 | 4.5 | 4.3 | 3.3 | 4.1 | 1.3 | 3.7 | 6.2 | 2.7 | 2.8 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3.2 | 5.6 | 0 | 5.2 | 1.5 | 0 | 0 | 0 | 0 | 3.5 | 5.2 | 6.6 | 2.4 | 1.6 | 0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 0 | 0 | 4.7 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.2 | 3.6 |
Nucleus | -7.8 | -6.6 | -8.0 | -7.0 | -7.9 | -9.5 | -8.1 | -7.7 | -9.9 | -7.9 | -11.5 | -11.6 | -5.3 | -5.9 | -6.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 7.5 | 1.9 | 0 | -2.8 | -5.1 | 0 | 0 | 0 | 0 | 7.1 | 6.6 | -0.1 | 1.3 | 1.7 | 4.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7.6 | 5.8 | 0 | 3.4 | 2.2 | 0 | 0 | 0 | 0 | 3.5 | 5.1 | 6.7 | 1.5 | 0.9 | 1.2 |
Vacuole | 0 | 2.4 | 1.2 | 3.4 | 3.1 | 4.2 | 3.3 | 2.5 | 4.1 | 0 | 0 | 0.9 | 2.2 | 3.4 | 2.3 |