Standard name
Description Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.05 0.12 0.17 0.27 0.22 0.2 0.16 0.19 0.08 0.17 0.29 0.13 0.13 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.15 0.27 0.05 0.26 0.09 0.07 0.14 0.11 0.14 0.16 0.24 0.31 0.12 0.09 0.06 0 0 0 0.11 0.18 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.51 0.13 0.24 0.52 0.35 0.46 0.37 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Nucleus 0.8 0.8 0.68 0.29 0.32 0.22 0.29 0.29 0.16 0.26 0.29 0.19 0.29 0.17 0.17 0.43 0.39 0.38 0.52 0.58 0.36 0.23 0.16 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.14 0.26 0.64 0.35 0.23 0.13 0.08 0.1 0.09 0.1 0.07 0.61 0.57 0.25 0.32 0.33 0.46 0.33 0.25 0.43 0.32 0.26 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.41 0.32 0.12 0.2 0.14 0.08 0.15 0.09 0.16 0.19 0.26 0.34 0.11 0.09 0.1 0 0 0.06 0.11 0.14 0.17
Vac/Vac Membrane 0 0.09 0 0 0.09 0.05 0.12 0.1 0.16 0.12 0.08 0.15 0 0 0 0.07 0.12 0.08 0.06 0.09 0.05 0.15 0.15 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 1 0 1 2 5 3 3 3 7 6 1 1 0 0 0 0 1 5 2 0 1 3 1
Bud Neck 0 0 0 3 2 0 0 2 1 1 0 0 0 2 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 1 0 8 3 5 5 3 4 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 6 8 7 8 17 28 66 36 42 35 46 22 57 102 42 35 19 2 0 0 1 2 3
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 10 9 3 0 0 0 1 0 0
Endosome 2 3 8 37 48 7 42 22 11 28 24 35 43 80 109 39 24 16 6 10 8 25 54 62
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 0 1 3 0
Mitochondria 9 11 0 16 2 70 21 60 86 72 103 89 5 3 2 18 16 19 0 7 3 5 7 7
Nucleus 235 318 111 71 56 30 48 71 27 54 64 47 78 56 58 144 101 96 178 259 68 53 48 51
Nuclear Periphery 8 6 3 1 0 1 1 0 1 0 0 1 0 3 0 2 2 1 6 2 4 3 5 7
Nucleolus 47 54 42 156 62 32 22 19 16 19 22 16 164 194 89 106 87 116 113 111 82 75 76 73
Peroxisomes 1 0 0 1 2 0 0 3 1 2 1 2 1 0 4 1 0 0 0 0 0 0 0 0
SpindlePole 0 4 11 100 56 16 32 34 13 32 21 40 51 89 120 37 24 26 4 5 11 26 42 52
Vac/Vac Membrane 13 34 4 4 15 7 20 26 26 24 19 36 4 9 14 25 31 20 22 39 8 36 44 43
Unique Cell Count 292 399 163 244 175 138 163 248 166 207 224 243 268 338 350 335 260 252 344 446 191 237 298 314
Labelled Cell Count 319 438 187 399 253 193 220 312 226 284 299 317 370 493 498 424 332 318 344 446 191 237 298 314


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.5 7.9 7.4 7.5 4.4 5.3 5.0 4.5 4.6 4.6 4.7 8.0 6.7 6.1 8.6 9.9 8.7 7.2 6.8 7.0
Std Deviation (1e-4) 1.4 1.4 1.4 1.4 5.1 1.7 1.7 1.5 1.4 1.3 1.0 1.2 1.9 1.5 1.7 2.0 2.0 2.2 1.4 1.5 2.1
Intensity Change (Log2) – – – -0.09 -0.08 -0.83 -0.57 -0.67 -0.82 -0.76 -0.78 -0.75 0.02 -0.25 -0.37 0.13 0.32 0.15 -0.13 -0.22 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -0.1 2.3 3.5 5.6 4.5 4.3 3.3 4.1 1.3 3.7 6.2 2.7 2.8 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 3.2 5.6 0 5.2 1.5 0 0 0 0 3.5 5.2 6.6 2.4 1.6 0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 0 0 4.7 6.8 0 0 0 0 0 0 0 3.0 3.2 3.6
Nucleus -7.8 -6.6 -8.0 -7.0 -7.9 -9.5 -8.1 -7.7 -9.9 -7.9 -11.5 -11.6 -5.3 -5.9 -6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 7.5 1.9 0 -2.8 -5.1 0 0 0 0 7.1 6.6 -0.1 1.3 1.7 4.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 7.6 5.8 0 3.4 2.2 0 0 0 0 3.5 5.1 6.7 1.5 0.9 1.2
Vacuole 0 2.4 1.2 3.4 3.1 4.2 3.3 2.5 4.1 0 0 0.9 2.2 3.4 2.3