Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.05 0.12 0.17 0.27 0.22 0.2 0.16 0.19 0.08 0.17 0.29 0.13 0.13 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.15 0.27 0.05 0.26 0.09 0.07 0.14 0.11 0.14 0.16 0.24 0.31 0.12 0.09 0.06 0 0 0 0.11 0.18 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.51 0.13 0.24 0.52 0.35 0.46 0.37 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Nucleus 0.8 0.8 0.68 0.29 0.32 0.22 0.29 0.29 0.16 0.26 0.29 0.19 0.29 0.17 0.17 0.43 0.39 0.38 0.52 0.58 0.36 0.23 0.16 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.14 0.26 0.64 0.35 0.23 0.13 0.08 0.1 0.09 0.1 0.07 0.61 0.57 0.25 0.32 0.33 0.46 0.33 0.25 0.43 0.32 0.26 0.24
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.41 0.32 0.12 0.2 0.14 0.08 0.15 0.09 0.16 0.19 0.26 0.34 0.11 0.09 0.1 0 0 0.06 0.11 0.14 0.17
Vac/Vac Membrane 0 0.09 0 0 0.09 0.05 0.12 0.1 0.16 0.12 0.08 0.15 0 0 0 0.07 0.12 0.08 0.06 0.09 0.05 0.15 0.15 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 1 0 1 2 5 3 3 3 7 6 1 1 0 0 0 0 1 5 2 0 1 3 1
Bud Neck 0 0 0 3 2 0 0 2 1 1 0 0 0 2 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 1 0 8 3 5 5 3 4 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 6 8 7 8 17 28 66 36 42 35 46 22 57 102 42 35 19 2 0 0 1 2 3
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 10 9 3 0 0 0 1 0 0
Endosome 2 3 8 37 48 7 42 22 11 28 24 35 43 80 109 39 24 16 6 10 8 25 54 62
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 0 1 3 0
Mitochondria 9 11 0 16 2 70 21 60 86 72 103 89 5 3 2 18 16 19 0 7 3 5 7 7
Nucleus 235 318 111 71 56 30 48 71 27 54 64 47 78 56 58 144 101 96 178 259 68 53 48 51
Nuclear Periphery 8 6 3 1 0 1 1 0 1 0 0 1 0 3 0 2 2 1 6 2 4 3 5 7
Nucleolus 47 54 42 156 62 32 22 19 16 19 22 16 164 194 89 106 87 116 113 111 82 75 76 73
Peroxisomes 1 0 0 1 2 0 0 3 1 2 1 2 1 0 4 1 0 0 0 0 0 0 0 0
SpindlePole 0 4 11 100 56 16 32 34 13 32 21 40 51 89 120 37 24 26 4 5 11 26 42 52
Vac/Vac Membrane 13 34 4 4 15 7 20 26 26 24 19 36 4 9 14 25 31 20 22 39 8 36 44 43
Unique Cell Count 292 399 163 244 175 138 163 248 166 207 224 243 268 338 350 335 260 252 344 446 191 237 298 314
Labelled Cell Count 319 438 187 399 253 193 220 312 226 284 299 317 370 493 498 424 332 318 344 446 191 237 298 314


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.5 7.9 7.4 7.5 4.4 5.3 5.0 4.5 4.6 4.6 4.7 8.0 6.7 6.1 8.6 9.9 8.7 7.2 6.8 7.0
Std Deviation (1e-4) 1.4 1.4 1.4 1.4 5.1 1.7 1.7 1.5 1.4 1.3 1.0 1.2 1.9 1.5 1.7 2.0 2.0 2.2 1.4 1.5 2.1
Intensity Change (Log2) -0.09 -0.08 -0.83 -0.57 -0.67 -0.82 -0.76 -0.78 -0.75 0.02 -0.25 -0.37 0.13 0.32 0.15 -0.13 -0.22 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -0.1 2.3 3.5 5.6 4.5 4.3 3.3 4.1 1.3 3.7 6.2 2.7 2.8 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 3.2 5.6 0 5.2 1.5 0 0 0 0 3.5 5.2 6.6 2.4 1.6 0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 0 0 4.7 6.8 0 0 0 0 0 0 0 3.0 3.2 3.6
Nucleus -7.8 -6.6 -8.0 -7.0 -7.9 -9.5 -8.1 -7.7 -9.9 -7.9 -11.5 -11.6 -5.3 -5.9 -6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 7.5 1.9 0 -2.8 -5.1 0 0 0 0 7.1 6.6 -0.1 1.3 1.7 4.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 7.6 5.8 0 3.4 2.2 0 0 0 0 3.5 5.1 6.7 1.5 0.9 1.2
Vacuole 0 2.4 1.2 3.4 3.1 4.2 3.3 2.5 4.1 0 0 0.9 2.2 3.4 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.7136 6.9055 6.3589 5.7118 6.0236 6.4152 5.3547 6.0961 5.5454 5.07 4.3647 5.8206 7.458 7.2939 6.6271 6.6585 6.6818 7.0299
Actin 0.0153 0 0.0268 0.0002 0.0271 0.0069 0.0665 0.0002 0.0048 0.0325 0.0021 0.0045 0.0263 0 0.0001 0.0103 0.0003 0.0131
Bud 0.0002 0.0001 0.0002 0.0005 0.0003 0.0001 0.0008 0.0002 0.0004 0.0004 0.0003 0.0001 0.0003 0.0001 0.0002 0.0003 0.0002 0.0001
Bud Neck 0.001 0.0014 0.0022 0.0019 0.0018 0.0017 0.0034 0.0017 0.0013 0.0029 0.0011 0.0024 0.0008 0.0015 0.0009 0.001 0.0008 0.0017
Bud Periphery 0.0005 0.0001 0.0004 0.0012 0.0007 0.0003 0.0019 0.0005 0.0014 0.001 0.0009 0.0005 0.0006 0.0002 0.0005 0.0005 0.0008 0.0003
Bud Site 0.0015 0.0006 0.0026 0.0009 0.0009 0.0003 0.0091 0.0013 0.002 0.0023 0.0012 0.0004 0.0009 0.001 0.001 0.0008 0.0004 0.0004
Cell Periphery 0.0003 0.0002 0.0003 0.0016 0.0002 0.0002 0.001 0.0005 0.0005 0.0007 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.0056 0.0003 0.0027 0.0243 0.0008 0.0002 0.0091 0.0007 0.0097 0.0007 0.0014 0.0004 0.0011 0.0034 0.0006 0.0009 0.0002 0.0001
Cytoplasmic Foci 0.0047 0.0002 0.0044 0.0007 0.0004 0.0004 0.0233 0.0003 0.01 0.001 0.0069 0.0009 0.0095 0.0004 0.0009 0.0045 0.0001 0.002
Eisosomes 0.0003 0 0.0005 0.0007 0.0003 0 0.0006 0 0.0001 0.0003 0.0001 0 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0014 0 0.0005 0.0016 0.0011 0.0001 0.005 0.0003 0.0015 0.0023 0.0027 0.0023 0.0013 0.0003 0.0009 0.0013 0.0001 0.0001
Endosome 0.0079 0.0003 0.0045 0.002 0.0037 0.001 0.0294 0.0017 0.0109 0.0071 0.0168 0.0046 0.0157 0.0025 0.0086 0.0104 0.0005 0.0016
Golgi 0.0032 0 0.0028 0.0001 0.0026 0.0026 0.0216 0.0001 0.0091 0.005 0.0055 0.002 0.0052 0 0.0005 0.0096 0.0001 0.0013
Lipid Particles 0.0084 0.0004 0.0051 0.0014 0.0014 0.0007 0.0222 0.0008 0.0084 0.0025 0.029 0.0098 0.0083 0.0004 0.0034 0.0121 0.0005 0.0046
Mitochondria 0.018 0.0001 0.0016 0.0007 0.003 0.0055 0.0239 0.0012 0.0101 0.0044 0.0746 0.0009 0.0252 0.0002 0.0019 0.0046 0.0024 0.0008
None 0.001 0 0.0109 0.0407 0.0043 0.0001 0.0143 0.0069 0.004 0.0004 0.0011 0.0002 0.0008 0.0019 0.0003 0.0005 0.0006 0.0001
Nuclear Periphery 0.0149 0.0015 0.006 0.0055 0.0443 0.0044 0.0067 0.0052 0.0082 0.0039 0.0138 0.0126 0.0213 0.0124 0.0057 0.0112 0.0077 0.0024
Nucleolus 0.4679 0.4168 0.528 0.3834 0.3265 0.5396 0.413 0.5723 0.6327 0.5406 0.333 0.608 0.4347 0.5171 0.6332 0.5209 0.2453 0.5205
Nucleus 0.424 0.5676 0.3666 0.5081 0.5255 0.4116 0.2927 0.3887 0.2253 0.3434 0.4586 0.3291 0.394 0.4351 0.3069 0.3539 0.6949 0.4204
Peroxisomes 0.0028 0 0.0018 0.0001 0.0003 0.0005 0.024 0.0001 0.0137 0.0004 0.0016 0.0013 0.0052 0 0.0001 0.0018 0.0002 0.0027
Punctate Nuclear 0.0185 0.0085 0.0262 0.0027 0.0516 0.0223 0.0213 0.0075 0.0351 0.034 0.0397 0.0171 0.0453 0.014 0.0136 0.053 0.0429 0.0267
Vacuole 0.0013 0.0016 0.0045 0.0185 0.0014 0.001 0.0081 0.0083 0.0072 0.0132 0.0043 0.0014 0.0015 0.0085 0.016 0.0012 0.0008 0.0007
Vacuole Periphery 0.0013 0.0002 0.0013 0.0032 0.0018 0.0003 0.0021 0.0014 0.0035 0.001 0.0051 0.0012 0.0017 0.0005 0.0045 0.0008 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 610.0122 705.7 884.2711 637.1796 768.8086 749.541 597.9889 618.948 742.4403 567.4522
Translational Efficiency 1.5479 1.4603 1.1759 1.7911 1.4388 1.2602 1.4881 1.5594 1.2303 1.5247

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
Localization
Cell Percentages nucleus (48%), nucleolus (16%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rpl15b

Rpl15b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl15b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available