Standard name
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.08 0.09 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.09 0 0.11 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 1.0 0.96 0.94 0.95 0.81 0.7 0.77 0.8 0.79 – 0.95 0.99 0.99 0.96 0.99 0.99 0.94 0.88 0.91 0.78 0.85 0.9
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.13 0.2 0.12 0.05 0.08 – 0.07 0 0 0 0 0 0 0 0.06 0 0.08 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0 0.07 0.13 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 4 8 8 3 1 – 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 3 3 3 3 6 8 3 4 – 0 1 2 3 6 2 0 0 0 0 0 1
Cytoplasm 62 57 25 78 114 97 104 69 69 68 30 – 52 94 153 196 228 207 83 66 65 28 76 131
Endoplasmic Reticulum 2 1 0 2 6 3 17 20 11 4 3 – 4 1 2 7 10 8 2 1 4 1 7 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 3 1 0 4 0 – 0 0 1 4 1 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 6 3 6 5 – 0 0 0 2 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 2 3 0 – 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 0 – 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 1 1 0 – 0 0 0 3 0 3 0 4 0 1 0 1
Unique Cell Count 64 58 25 81 121 102 129 99 90 85 38 55 95 155 205 231 210 89 75 72 36 91 147
Labelled Cell Count 64 61 26 83 123 104 132 110 102 95 43 56 96 159 215 245 222 89 75 72 36 91 147


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 73.6 110.2 72.5 65.7 58.2 44.5 40.5 40.8 33.9 34.5 35.0 – 77.7 77.1 76.2 118.7 135.4 130.1 – 76.5 84.4
Std Deviation (1e-4) 14.0 28.1 20.4 18.3 14.4 11.1 10.7 12.9 13.9 11.6 13.2 – 18.6 20.7 21.8 41.5 40.5 40.0 – 23.8 22.8
Intensity Change (Log2) – – – -0.14 -0.32 -0.7 -0.84 -0.83 -1.1 -1.07 -1.05 – 0.1 0.09 0.07 0.71 0.9 0.84 – 0.08 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm -1.0 -1.2 -1.1 -2.4 -3.2 -2.7 -2.4 -2.5 – -1.2 -0.5 -0.6 -1.1 -0.6 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 52.8726 105.2113 89.1982 78.0593 76.2238 88.0607 87.2053 130.7961 105.1877 86.8347 115.8124 99.8349 109.1815 147.8694 137.4147 125.4146 115.6374 133.8221
Actin 0.0224 0.0005 0.0367 0.0005 0.0005 0.0055 0.0048 0.0003 0.0087 0.0019 0.0002 0.0016 0.006 0.0005 0.0024 0.0003 0.0007 0.0007
Bud 0.0041 0.0007 0.0038 0.0023 0.0033 0.005 0.0037 0.0006 0.0046 0.0043 0.0011 0.0017 0.0031 0.0021 0.0019 0.0012 0.0019 0.0018
Bud Neck 0.0018 0.0007 0.0014 0.0005 0.0011 0.0038 0.001 0.0007 0.001 0.001 0.0012 0.0012 0.001 0.0006 0.0009 0.0006 0.0004 0.0024
Bud Periphery 0.0065 0.0009 0.0035 0.0016 0.0013 0.0056 0.0032 0.0004 0.0043 0.0063 0.0006 0.0016 0.0051 0.0019 0.0019 0.0007 0.0023 0.0019
Bud Site 0.0069 0.0009 0.006 0.001 0.0005 0.0015 0.0026 0.0007 0.0056 0.0177 0.0001 0.0003 0.002 0.0009 0.0011 0.0008 0.0003 0.0005
Cell Periphery 0.0185 0.036 0.0354 0.0269 0.0073 0.0338 0.0345 0.0126 0.0264 0.0087 0.0006 0.0157 0.0598 0.0384 0.0484 0.0643 0.0228 0.031
Cytoplasm 0.55 0.8563 0.721 0.6087 0.7497 0.6731 0.5882 0.8887 0.734 0.4403 0.7242 0.701 0.5853 0.8256 0.7336 0.5093 0.5332 0.7412
Cytoplasmic Foci 0.0403 0.001 0.0047 0.0256 0.0107 0.0114 0.0139 0.0012 0.0036 0.0035 0.0035 0.0398 0.005 0.0008 0.0017 0.0048 0.0021 0.0037
Eisosomes 0.0017 0 0.0004 0.0009 0.0002 0.0001 0.0006 0 0.0002 0.0002 0 0 0.0009 0.0001 0.0003 0.0009 0 0
Endoplasmic Reticulum 0.034 0.0061 0.0061 0.0021 0.0021 0.0126 0.0498 0.0096 0.0091 0.007 0.0094 0.0242 0.0481 0.0085 0.0143 0.0013 0.0092 0.0305
Endosome 0.0362 0.0019 0.0155 0.0064 0.0107 0.0557 0.0161 0.0026 0.0103 0.0585 0.0167 0.0385 0.0095 0.0018 0.0055 0.0056 0.0221 0.0241
Golgi 0.0065 0.0006 0.0048 0.0005 0.0006 0.0107 0.0029 0.0004 0.0014 0.0032 0.0004 0.0274 0.0021 0.0003 0.0025 0.0004 0.0012 0.0037
Lipid Particles 0.0127 0.0024 0.003 0.0141 0.0018 0.017 0.0069 0.001 0.0019 0.0031 0.0001 0.0027 0.0041 0.0016 0.0052 0.0088 0.0014 0.0023
Mitochondria 0.0173 0.0007 0.0033 0.006 0.0063 0.0205 0.0116 0.0002 0.0031 0.0303 0.0005 0.0111 0.0224 0.0012 0.0045 0.0032 0.0095 0.0022
None 0.0478 0.0003 0.0016 0.073 0.0329 0.0044 0.0127 0.0002 0.0023 0.0015 0.0002 0.0016 0.0039 0.0002 0.0005 0.0077 0.0006 0.0007
Nuclear Periphery 0.0353 0.0034 0.006 0.0065 0.0036 0.028 0.0366 0.0019 0.0043 0.0476 0.0051 0.0047 0.0419 0.0029 0.0105 0.0108 0.0221 0.0076
Nucleolus 0.0091 0.0005 0.0012 0.0176 0.0133 0.0016 0.0049 0.0002 0.0027 0.0053 0.0001 0.0005 0.0042 0.0003 0.0007 0.0131 0.0007 0.0006
Nucleus 0.0222 0.0023 0.0054 0.0086 0.0133 0.0056 0.0126 0.0018 0.0058 0.0143 0.0025 0.0028 0.0117 0.0021 0.0037 0.0116 0.0063 0.0039
Peroxisomes 0.0015 0 0.0003 0.0007 0.0005 0.0017 0.0007 0 0.0002 0.0006 0.0001 0.0003 0.0004 0 0.0001 0.0003 0.0001 0.0001
Punctate Nuclear 0.0123 0 0.0003 0.002 0.0006 0.0126 0.0011 0 0.0001 0.0006 0 0.0049 0.0006 0 0.0001 0.0004 0.0001 0.0001
Vacuole 0.096 0.0804 0.1234 0.1611 0.1204 0.0698 0.172 0.0729 0.1469 0.2496 0.1898 0.0935 0.1559 0.1042 0.1482 0.2813 0.2828 0.1087
Vacuole Periphery 0.017 0.0045 0.0161 0.0334 0.0195 0.0201 0.0196 0.0042 0.0234 0.0945 0.0435 0.0247 0.027 0.0059 0.0119 0.0725 0.0802 0.0323

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1388.1952 1269.5027 1599.4106 1542.1548 1650.8336 1110.4519 1284.17 1247.3095 1335.9248 1324.7944
Translational Efficiency 1.4925 1.6186 1.4436 1.2159 1.4209 2.0288 1.6465 1.5947 1.5831 1.8119

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘