Standard name
Description Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0.11 0.09 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0.07 0.08 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0
Golgi 0 0.13 0.41 0.25 0.33 0.06 0 0 0 0 0 0.49 0.45 0.62 0.51 0.41 0 0.12 0 0 0.09 0.13
Mitochondria 0.92 0.80 0.62 0.89 0.67 0.76 0.75 0.81 0.81 0.89 0.88 0.67 0.70 0.09 0.05 0.06 0.85 0.75 0.87 0.79 0.62 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.08 0.06 0.07 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.06 0.05 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.25 0.29 0.10 0.23 0.17 0.12 0.10 0.09 0.08 0.08 0.20 0.16 0.33 0.24 0.25 0 0 0 0 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 5 4 0 1 1 0 0 0 2 2 1 1 4 2 2 2 0 3 0
Bud 3 2 1 3 2 5 8 10 5 5 10 0 0 0 3 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 0 1 2 0 0 0 0 1
Cell Periphery 4 10 9 18 7 9 4 3 5 7 5 4 3 5 9 9 0 0 0 0 0 0
Cytoplasm 3 4 3 9 8 9 25 22 11 16 12 2 3 3 8 16 1 3 0 0 0 0
Endoplasmic Reticulum 2 2 1 1 1 1 6 1 1 3 2 0 1 3 3 4 0 2 0 0 1 1
Endosome 0 2 3 4 7 21 29 12 11 3 5 0 2 0 1 1 7 3 4 1 4 1
Golgi 4 27 60 69 85 23 22 5 8 8 9 50 74 28 53 45 9 26 3 0 5 6
Mitochondria 264 164 91 250 174 305 369 263 277 426 365 68 115 4 5 7 245 170 150 13 39 29
Nucleus 2 2 2 0 4 5 5 3 7 4 6 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 18 1 2 2 4 31 29 23 23 32 35 2 1 0 2 2 0 1 0 0 0 0
Nucleolus 14 3 2 2 10 18 30 17 24 33 21 0 3 1 4 2 2 1 0 0 0 0
Peroxisomes 1 4 2 3 1 1 9 3 0 1 3 2 1 0 2 6 9 2 3 0 0 0
SpindlePole 1 1 4 0 1 3 9 1 8 3 4 0 0 1 0 1 1 0 0 0 0 0
Vac/Vac Membrane 53 52 43 27 59 67 60 33 31 36 33 20 26 15 25 27 5 8 3 0 5 7
Unique Cell Count 286 204 146 280 258 400 492 325 340 479 417 102 164 45 103 110 290 226 173 17 63 50
Labelled Cell Count 370 275 224 393 367 498 607 399 411 578 512 150 231 61 117 126 290 226 173 17 63 50


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 11.5 9.7 9.8 10.3 8.1 7.9 6.9 7.4 7.4 7.6 14.3 12.4 12.6 11.1 11.0 13.9
Std Deviation (1e-4) 1.3 4.1 1.6 1.8 2.5 1.9 2.6 1.8 2.0 2.0 1.9 3.3 2.7 4.2 2.8 3.3 6.3
Intensity Change (Log2) 0.02 0.08 -0.26 -0.3 -0.5 -0.39 -0.4 -0.35 0.55 0.35 0.38 0.19 0.18 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -1.7 0 0 0 0 0 0 -0.8 -2.0 0 0.8 0.6
Cytoplasm 0 0 0 1.6 2.1 0 0 0 0 0 0 0 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 1.6 1.9 0 0 0 0 0 0 0 0 0
Golgi -3.5 -1.6 -10.2 -11.6 -11.5 -11.3 -13.4 -12.3 1.2 0.7 2.5 1.6 0
Mitochondria 6.6 1.0 3.2 3.0 4.3 4.5 7.4 6.7 0.7 1.5 -6.3 -9.2 -9.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.8 2.2 2.6 2.5 2.5 2.9 0 0 0 0 0
Nucleolus 0 0 1.7 2.3 2.0 2.6 2.5 1.9 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.2 -1.5 -3.3 -5.0 -5.3 -5.7 -7.0 -6.6 -1.8 -2.9 0.5 -0.9 -0.9