Standard name
Human Ortholog
Description Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0.11 0.09 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0.07 0.08 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0
Golgi 0 0.13 0.41 0.25 0.33 0.06 0 0 0 0 0 0.49 0.45 0.62 0.51 0.41 0 0.12 0 0 0.09 0.13
Mitochondria 0.92 0.8 0.62 0.89 0.67 0.76 0.75 0.81 0.81 0.89 0.88 0.67 0.7 0.09 0.05 0.06 0.85 0.75 0.87 0.79 0.62 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.08 0.06 0.07 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.06 0.05 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.25 0.29 0.1 0.23 0.17 0.12 0.1 0.09 0.08 0.08 0.2 0.16 0.33 0.24 0.25 0 0 0 0 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 5 4 0 1 1 0 0 0 2 2 1 1 4 2 2 2 0 3 0
Bud 3 2 1 3 2 5 8 10 5 5 10 0 0 0 3 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 0 1 2 0 0 0 0 1
Cell Periphery 4 10 9 18 7 9 4 3 5 7 5 4 3 5 9 9 0 0 0 0 0 0
Cytoplasm 3 4 3 9 8 9 25 22 11 16 12 2 3 3 8 16 1 3 0 0 0 0
Endoplasmic Reticulum 2 2 1 1 1 1 6 1 1 3 2 0 1 3 3 4 0 2 0 0 1 1
Endosome 0 2 3 4 7 21 29 12 11 3 5 0 2 0 1 1 7 3 4 1 4 1
Golgi 4 27 60 69 85 23 22 5 8 8 9 50 74 28 53 45 9 26 3 0 5 6
Mitochondria 264 164 91 250 174 305 369 263 277 426 365 68 115 4 5 7 245 170 150 13 39 29
Nucleus 2 2 2 0 4 5 5 3 7 4 6 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 18 1 2 2 4 31 29 23 23 32 35 2 1 0 2 2 0 1 0 0 0 0
Nucleolus 14 3 2 2 10 18 30 17 24 33 21 0 3 1 4 2 2 1 0 0 0 0
Peroxisomes 1 4 2 3 1 1 9 3 0 1 3 2 1 0 2 6 9 2 3 0 0 0
SpindlePole 1 1 4 0 1 3 9 1 8 3 4 0 0 1 0 1 1 0 0 0 0 0
Vac/Vac Membrane 53 52 43 27 59 67 60 33 31 36 33 20 26 15 25 27 5 8 3 0 5 7
Unique Cell Count 286 204 146 280 258 400 492 325 340 479 417 102 164 45 103 110 290 226 173 17 63 50
Labelled Cell Count 370 275 224 393 367 498 607 399 411 578 512 150 231 61 117 126 290 226 173 17 63 50


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 11.5 9.7 9.8 10.3 8.1 7.9 6.9 7.4 7.4 7.6 14.3 12.4 12.6 11.1 11.0 13.9
Std Deviation (1e-4) 1.3 4.1 1.6 1.8 2.5 1.9 2.6 1.8 2.0 2.0 1.9 3.3 2.7 4.2 2.8 3.3 6.3
Intensity Change (Log2) 0.02 0.08 -0.26 -0.3 -0.5 -0.39 -0.4 -0.35 0.55 0.35 0.38 0.19 0.18 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -1.7 0 0 0 0 0 0 -0.8 -2.0 0 0.8 0.6
Cytoplasm 0 0 0 1.6 2.1 0 0 0 0 0 0 0 3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 1.6 1.9 0 0 0 0 0 0 0 0 0
Golgi -3.5 -1.6 -10.2 -11.6 -11.5 -11.3 -13.4 -12.3 1.2 0.7 2.5 1.6 0
Mitochondria 6.6 1.0 3.2 3.0 4.3 4.5 7.4 6.7 0.7 1.5 -6.3 -9.2 -9.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.8 2.2 2.6 2.5 2.5 2.9 0 0 0 0 0
Nucleolus 0 0 1.7 2.3 2.0 2.6 2.5 1.9 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.2 -1.5 -3.3 -5.0 -5.3 -5.7 -7.0 -6.6 -1.8 -2.9 0.5 -0.9 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.6652 21.4915 21.9706 19.7251 18.2644 22.111 22.8501 24.312 22.2515 17.9587 21.2192 22.1901 21.1986 19.4219 22.7589 20.012 17.4753 18.2201
Actin 0.0013 0.0009 0.0007 0 0.0001 0.0013 0.0102 0.001 0.0092 0.0268 0.0021 0.0003 0.0354 0.0048 0.0182 0.0046 0.0003 0.0008
Bud 0 0.0001 0.0001 0 0 0.0001 0.0002 0.0001 0.0008 0.0016 0 0.0001 0.0005 0.0009 0.0003 0.0002 0.0001 0
Bud Neck 0 0 0 0 0 0 0.0002 0 0.0008 0.0008 0 0 0.0004 0.0005 0 0.0004 0.0001 0
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0004 0.0002 0.0017 0.0019 0.0001 0.0002 0.0007 0.0012 0.001 0.0005 0.0002 0
Bud Site 0.0001 0.0001 0.0003 0 0 0.0001 0.0008 0.0002 0.0034 0.0058 0.0002 0.0001 0.0028 0.0183 0.0008 0.0009 0.0001 0.0001
Cell Periphery 0.0002 0.0004 0.0003 0.0001 0.0004 0.0001 0.0003 0.0004 0.0005 0.0011 0.0001 0.0001 0.0003 0.0021 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0 0 0.0001 0 0 0 0.0007 0 0.0008 0.0024 0.0006 0 0.0006 0 0 0 0.0001 0
Cytoplasmic Foci 0.0008 0.0004 0.0058 0 0.0001 0.0002 0.0131 0.0003 0.0021 0.0349 0.0077 0.0001 0.0124 0.0004 0.0003 0.0038 0.0015 0.0004
Eisosomes 0.0002 0.0003 0.0002 0.0001 0.0002 0.0001 0.0004 0.001 0.0005 0.0017 0.0003 0.0001 0.0003 0.0003 0.0008 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0 0 0.0002 0 0 0 0.0011 0 0.0009 0.0022 0.0002 0 0.0004 0 0.0002 0.0001 0.0003 0
Endosome 0.0007 0.0013 0.0016 0.0002 0.0033 0.0011 0.0052 0.002 0.0059 0.0355 0.0081 0.0002 0.0083 0.0014 0.0009 0.0043 0.0392 0.0004
Golgi 0.0041 0.0034 0.001 0.0011 0.005 0.0046 0.0044 0.0068 0.0096 0.0258 0.0016 0.0004 0.01 0.0026 0.002 0.0071 0.0245 0.0011
Lipid Particles 0.0045 0.0041 0.0051 0.0005 0.0007 0.002 0.0173 0.004 0.0063 0.0144 0.0113 0.0006 0.0548 0.0063 0.0029 0.0431 0.0186 0.0048
Mitochondria 0.9763 0.9766 0.9578 0.994 0.9774 0.9761 0.9145 0.9559 0.9301 0.7961 0.9425 0.9919 0.8221 0.9102 0.9677 0.9114 0.8411 0.9864
None 0.0001 0 0.0007 0 0 0 0.0007 0 0.0021 0.004 0.0013 0 0.0031 0 0.0002 0.0001 0.0001 0
Nuclear Periphery 0 0 0.0004 0 0 0.0001 0.0049 0.0001 0.0029 0.0016 0.0025 0 0.0009 0.0001 0.0007 0.0001 0.0006 0
Nucleolus 0.0024 0.0002 0.0018 0 0 0 0.0015 0.0002 0.0014 0.0016 0.0005 0.0001 0.0045 0.0016 0.0001 0.0008 0.0004 0.0001
Nucleus 0.0003 0.0001 0.0007 0 0 0 0.0005 0.0001 0.0017 0.0006 0.0005 0 0.0006 0.0005 0.0001 0.0003 0.0003 0
Peroxisomes 0.0013 0.0022 0.0027 0.0001 0.0001 0.0004 0.0067 0.0016 0.0085 0.0322 0.0072 0.001 0.0211 0.0025 0.0012 0.0176 0.0008 0.0034
Punctate Nuclear 0.0001 0 0.0009 0 0 0 0.0004 0 0.0006 0.0037 0.0115 0 0.0028 0 0.0001 0.0004 0 0
Vacuole 0.0014 0.0015 0.0044 0.0003 0.0019 0.0009 0.0022 0.0042 0.0038 0.0021 0.0003 0.0009 0.003 0.0246 0.0003 0.0012 0.0163 0.0003
Vacuole Periphery 0.0062 0.008 0.0152 0.0035 0.0104 0.0125 0.0145 0.0219 0.0062 0.0031 0.0014 0.0042 0.015 0.0216 0.0018 0.0029 0.055 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.0719 98.4618 101.7662 110.3813 76.5033 71.4228 110.9619 114.5875 115.452 92.159
Translational Efficiency 0.9675 1.1278 1.2491 1.3073 1.041 0.8644 1.1044 1.1691 1.0577 1.034

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1072 1209 836 566 1774 1753 207 623 2846 2962 1043 1189

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.25 926.30 1261.41 1393.68 759.37 939.21 1264.65 1450.73 709.60 933.94 1262.05 1423.57
Standard Deviation 157.16 164.72 203.79 301.19 106.96 164.25 202.72 282.36 143.29 164.56 203.58 292.86
Intensity Change Log 2 0.562439 1.007925 1.151787 0.306645 0.735863 0.933904 0.427998 0.865316 1.036557

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001448 0.001665 0.001871 0.006151 0.000671 0.001193 0.004200 0.003651 0.000964 0.001386 0.002333 0.004841
Bud Neck 0.016229 0.001389 0.006650 0.004866 0.004485 0.001059 0.005665 0.003288 0.008909 0.001193 0.006455 0.004039
Bud Site 0.015129 0.004903 0.011699 0.022258 0.011056 0.003634 0.027527 0.012206 0.012590 0.004152 0.014840 0.016991
Cell Periphery 0.001417 0.000416 0.000214 0.001141 0.000512 0.000471 0.000479 0.001118 0.000853 0.000449 0.000267 0.001129
Cytoplasm 0.012796 0.000695 0.001031 0.003150 0.004566 0.001979 0.001802 0.001153 0.007666 0.001455 0.001184 0.002104
Cytoplasmic Foci 0.053436 0.013126 0.033417 0.010911 0.037366 0.011468 0.059966 0.010278 0.043419 0.012145 0.038686 0.010579
Eisosomes 0.000149 0.000054 0.000069 0.000243 0.000060 0.000050 0.000078 0.000205 0.000094 0.000051 0.000071 0.000223
Endoplasmic Reticulum 0.001467 0.000328 0.000442 0.001313 0.000578 0.000303 0.001493 0.000885 0.000913 0.000313 0.000651 0.001088
Endosome 0.048972 0.028520 0.027195 0.018176 0.063991 0.020135 0.064303 0.011749 0.058334 0.023557 0.034559 0.014809
Golgi 0.054457 0.022952 0.052945 0.065826 0.103122 0.022963 0.115835* 0.058156 0.084792 0.022958 0.065427 0.061807
Lipid Particles 0.016590 0.001741 0.002723 0.001667 0.001407 0.001929 0.002183 0.002816 0.007126 0.001852 0.002616 0.002269
Mitochondria 0.681615* 0.881578* 0.744277* 0.760613* 0.694692* 0.901934* 0.575779* 0.772116* 0.689766* 0.893625* 0.710836* 0.766640*
Mitotic Spindle 0.007159 0.003293 0.024848 0.013489 0.012663 0.002410 0.044480 0.006035 0.010590 0.002771 0.028744 0.009583
None 0.003409 0.000332 0.000956 0.001721 0.000620 0.000398 0.000558 0.002568 0.001670 0.000371 0.000877 0.002165
Nuclear Periphery 0.000501 0.000088 0.001928 0.001033 0.000123 0.000195 0.001228 0.001910 0.000265 0.000152 0.001789 0.001492
Nuclear Periphery Foci 0.000852 0.000208 0.001456 0.000913 0.000398 0.000221 0.001186 0.000967 0.000569 0.000216 0.001402 0.000941
Nucleolus 0.001805 0.000848 0.001021 0.000554 0.001059 0.000158 0.000310 0.002505 0.001340 0.000440 0.000880 0.001576
Nucleus 0.009142 0.000468 0.000443 0.000369 0.001423 0.000451 0.001692 0.001007 0.004330 0.000458 0.000691 0.000703
Peroxisomes 0.040977 0.016981 0.014071 0.005578 0.020362 0.008222 0.013494 0.003329 0.028127 0.011797 0.013956 0.004400
Vacuole 0.014899 0.010162 0.028135 0.032680 0.016379 0.008723 0.036757 0.035100 0.015822 0.009311 0.029846 0.033948
Vacuole Periphery 0.017550 0.010253 0.044609 0.047347 0.024468 0.012103 0.040984 0.068959 0.021862 0.011348 0.043890 0.058671

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.54 -1.35 -2.99 -2.82 -2.72 -1.80 -3.86 -2.44 -1.97 0.36 -1.79 -4.97 -3.94 -3.45 -2.49
Bud Neck 6.73 3.95 4.67 -3.42 1.33 4.40 -0.80 1.11 -3.20 1.60 7.97 1.88 4.24 -4.70 2.46
Bud Site 5.00 1.19 -1.63 -4.07 -2.28 6.11 -2.26 -0.53 -3.01 1.90 7.78 -1.04 -1.79 -5.10 -0.71
Cell Periphery 3.68 4.57 0.71 -2.45 -3.21 0.22 0.21 -1.59 -1.65 -1.69 2.68 4.75 -1.05 -2.70 -3.65
Cytoplasm 8.40 8.13 5.26 -2.15 -1.84 2.54 3.50 2.82 0.37 0.23 7.56 8.97 5.45 -1.13 -1.69
Cytoplasmic Foci 11.35 4.63 11.71 1.16 7.12 12.89 -2.74 12.62 0.63 6.05 17.05 1.51 17.23 1.16 9.47
Eisosomes 8.99 7.16 -2.49 -5.13 -4.68 1.85 -1.85 -4.61 -4.91 -3.88 7.82 3.63 -5.33 -7.11 -6.23
Endoplasmic Reticulum 4.46 3.96 0.40 -3.67 -3.21 3.04 -1.59 -1.35 -2.60 1.07 5.39 1.42 -0.89 -4.46 -1.93
Endosome 5.38 5.69 7.99 3.15 2.75 13.95 -0.03 16.07 3.57 7.21 14.27 7.59 17.28 4.46 7.14
Golgi 7.84 0.27 -1.52 -6.16 -1.64 18.53* -0.92 6.62 -6.10 4.09 19.81 3.78 4.48 -8.67 0.60
Lipid Particles 7.64 7.08 7.74 0.14 2.07 -1.13 -1.53 -1.69 -0.97 -0.67 6.63 5.47 5.62 -0.79 0.60
Mitochondria -16.35 -4.10 -4.59 7.90 -0.93 -22.35* 4.43 -5.19 9.80 -6.83 -27.54* -1.67 -6.83 12.66 -3.95
Mitotic Spindle 3.04 -5.66 -2.10 -3.48 2.70 7.67* -3.52 3.46 -2.45 4.22 8.13 -5.92 0.45 -4.27 5.67
None 3.79 3.03 2.08 -11.42 -6.00 1.04 0.32 -6.58 -10.15 -9.40 3.89 2.40 -1.33 -14.07 -10.02
Nuclear Periphery 2.75 -3.87 -2.30 -5.43 2.39 -1.11 -3.86 -3.62 -3.47 -1.19 1.66 -5.38 -4.42 -4.92 0.81
Nuclear Periphery Foci 1.70 -1.31 -0.09 -3.86 1.81 1.08 -2.40 -1.99 -3.25 0.63 2.01 -2.96 -1.55 -4.91 1.86
Nucleolus 1.40 1.28 2.38 0.53 1.30 2.45 1.92 -1.02 -1.77 -1.64 2.57 1.09 -0.28 -1.55 -0.86
Nucleus 4.66 4.70 4.74 0.38 0.46 1.94 -0.37 0.44 -0.99 0.53 5.04 4.36 4.46 -0.79 0.02
Peroxisomes 5.99 6.93 9.46 4.18 3.43 5.81 1.88 9.35 3.26 3.00 8.10 6.04 12.74 5.04 5.13
Vacuole 2.22 -4.69 -4.24 -5.56 -0.97 6.00 -4.19 -5.05 -7.19 0.33 5.74 -6.27 -6.56 -9.08 -1.16
Vacuole Periphery 4.04 -7.18 -6.19 -7.77 -0.47 6.98 -2.99 -7.66 -9.92 -3.61 8.12 -6.93 -9.61 -12.52 -3.10