Standard name | |
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Human Ortholog | |
Description | FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 2 | 1 | 5 | 1 | 2 | 2 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 2 | 9 | 2 | 13 | 10 | 14 | 11 | 6 | 11 | 9 | 7 | 38 | 38 | 54 | 8 | 12 | 5 | 1 | 0 | 0 | 2 | 1 | 1 |
Endoplasmic Reticulum | 0 | 1 | 4 | 1 | 1 | 3 | 2 | 3 | 1 | 1 | 2 | 4 | 2 | 6 | 4 | 1 | 3 | 2 | 0 | 0 | 0 | 2 | 3 | 2 |
Endosome | 3 | 0 | 13 | 9 | 16 | 15 | 12 | 25 | 3 | 4 | 1 | 8 | 2 | 2 | 6 | 3 | 4 | 2 | 5 | 1 | 5 | 7 | 20 | 26 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 8 | 9 |
Mitochondria | 8 | 15 | 38 | 35 | 31 | 139 | 172 | 173 | 127 | 186 | 203 | 166 | 4 | 4 | 4 | 10 | 21 | 5 | 5 | 2 | 7 | 29 | 56 | 82 |
Nucleus | 18 | 6 | 5 | 6 | 9 | 16 | 18 | 22 | 12 | 17 | 26 | 14 | 32 | 32 | 20 | 7 | 9 | 3 | 4 | 3 | 3 | 1 | 5 | 4 |
Nuclear Periphery | 202 | 91 | 308 | 214 | 213 | 346 | 383 | 487 | 228 | 278 | 300 | 342 | 307 | 311 | 292 | 105 | 115 | 71 | 257 | 91 | 346 | 276 | 455 | 496 |
Nucleolus | 11 | 4 | 47 | 65 | 37 | 114 | 134 | 209 | 117 | 151 | 160 | 199 | 14 | 14 | 6 | 7 | 19 | 8 | 2 | 0 | 1 | 2 | 3 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 9 |
SpindlePole | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 5 | 1 | 2 | 2 | 1 | 7 | 8 | 18 | 0 | 2 | 0 | 2 | 0 | 1 | 2 | 1 | 2 |
Vac/Vac Membrane | 88 | 26 | 60 | 25 | 45 | 48 | 33 | 40 | 24 | 24 | 16 | 26 | 102 | 104 | 142 | 56 | 59 | 36 | 10 | 6 | 5 | 9 | 16 | 23 |
Unique Cell Count | 257 | 108 | 354 | 254 | 285 | 501 | 551 | 704 | 373 | 469 | 490 | 514 | 410 | 415 | 430 | 150 | 181 | 94 | 296 | 110 | 376 | 343 | 586 | 678 |
Labelled Cell Count | 333 | 146 | 484 | 358 | 370 | 695 | 772 | 983 | 525 | 679 | 726 | 771 | 510 | 521 | 558 | 197 | 247 | 133 | 296 | 110 | 376 | 343 | 586 | 678 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.2 | 9.1 | 9.7 | 9.5 | 8.9 | 7.9 | 7.8 | 8.2 | 7.7 | 7.5 | 7.6 | 8.3 | 8.9 | 8.9 | 9.1 | 9.4 | 10.3 | 13.0 | 9.2 | 10.3 | 10.5 |
Std Deviation (1e-4) | 2.2 | 1.9 | 2.5 | 2.8 | 2.4 | 2.1 | 2.1 | 2.1 | 2.3 | 2.4 | 2.3 | 2.9 | 2.1 | 2.1 | 2.4 | 2.0 | 3.0 | 3.6 | 1.9 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.11 | -0.29 | -0.31 | -0.24 | -0.33 | -0.37 | -0.34 | -0.23 | -0.12 | -0.12 | -0.09 | -0.04 | 0.1 | 0.43 | -0.08 | 0.09 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.4 | -0.5 | 0 | -1.1 | -0.9 | -0.2 | -0.7 | -1.3 | 3.9 | 3.8 | 5.1 | 1.6 | 2.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.1 | 1.2 | -0.5 | -1.3 | -0.1 | -2.6 | -2.8 | -3.9 | -2.0 | -3.2 | -3.2 | -2.1 | 0 | -0.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.1 | 0.1 | 6.0 | 7.1 | 5.3 | 7.5 | 9.2 | 9.7 | 7.4 | -5.9 | -5.9 | -6.1 | -1.4 | 0.3 | -1.6 |
Nucleus | 0 | 1.5 | 1.7 | 1.7 | 1.7 | 1.6 | 1.9 | 3.0 | 1.3 | 4.1 | 4.1 | 2.6 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | -4.0 | -6.1 | -6.0 | -6.3 | -7.9 | -8.7 | -8.2 | -6.8 | -4.2 | -4.2 | -6.3 | -4.5 | -6.3 | -2.7 |
Nucleolus | 3.9 | -0.1 | 3.5 | 4.1 | 5.9 | 5.8 | 6.3 | 6.5 | 8.2 | -5.0 | -5.1 | -6.6 | -2.9 | -0.9 | -1.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 |
Vacuole | -2.5 | -0.4 | -3.2 | -5.3 | -5.9 | -4.4 | -5.6 | -6.9 | -5.8 | 2.7 | 2.7 | 5.1 | 5.0 | 4.1 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5217 | 7.4632 | 6.7619 | 6.3754 | 7.2331 | 6.7118 | 9.1812 | 9.4465 | 8.8883 | 8.6795 | 8.7619 | 8.8441 | 10.2631 | 9.5296 | 9.2023 | 9.57 | 7.7167 | 9.1844 |
Actin | 0.0084 | 0 | 0.0102 | 0.0004 | 0.0008 | 0.0039 | 0.008 | 0.0001 | 0.0203 | 0.0114 | 0.0021 | 0.0018 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0005 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0007 | 0 | 0.0046 | 0 | 0 | 0.0002 | 0.0006 | 0 | 0.0007 | 0.0012 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.002 | 0 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0039 | 0 | 0.0009 | 0.0004 | 0.0067 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0049 | 0 | 0.004 | 0.0007 | 0.0001 | 0.0012 | 0.0074 | 0 | 0.0026 | 0.0079 | 0.0013 | 0.0007 | 0 | 0 | 0.0013 | 0 | 0 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0003 | 0.0008 | 0.0016 | 0.0013 | 0.0004 | 0.0042 | 0.0006 | 0.0016 | 0.0012 | 0.0014 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0015 | 0.0003 | 0.0001 |
Endosome | 0.0071 | 0.0001 | 0.0021 | 0.0014 | 0.0014 | 0.003 | 0.0131 | 0.0003 | 0.0053 | 0.0041 | 0.007 | 0.001 | 0.0001 | 0.0001 | 0.0024 | 0.0005 | 0.0003 | 0.0001 |
Golgi | 0.0022 | 0 | 0.0011 | 0.0004 | 0.0035 | 0.0009 | 0.0038 | 0.0001 | 0.0021 | 0.0039 | 0.0008 | 0.001 | 0.0001 | 0 | 0.0012 | 0.0002 | 0.0002 | 0.0002 |
Lipid Particles | 0.0061 | 0.0001 | 0.0076 | 0.0076 | 0.0045 | 0.0042 | 0.0135 | 0.002 | 0.0155 | 0.0066 | 0.0025 | 0.0027 | 0.0005 | 0.0002 | 0.0063 | 0.0007 | 0.0031 | 0.0097 |
Mitochondria | 0.0014 | 0.0002 | 0.0009 | 0.001 | 0.0053 | 0.0013 | 0.0044 | 0.0132 | 0.0022 | 0.0181 | 0.0167 | 0.0005 | 0.0007 | 0.0012 | 0.0014 | 0.0019 | 0.0003 | 0.0002 |
None | 0.0022 | 0 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0017 | 0.0001 | 0.0008 | 0.0008 | 0.0077 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 |
Nuclear Periphery | 0.91 | 0.9519 | 0.9108 | 0.9421 | 0.9449 | 0.9459 | 0.8876 | 0.928 | 0.8994 | 0.8818 | 0.8917 | 0.9719 | 0.9697 | 0.9318 | 0.944 | 0.9509 | 0.9667 | 0.9726 |
Nucleolus | 0.0015 | 0.0006 | 0.0036 | 0.0004 | 0.0005 | 0.0013 | 0.0006 | 0.0003 | 0.0009 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0002 |
Nucleus | 0.012 | 0.0259 | 0.0123 | 0.0109 | 0.0036 | 0.0188 | 0.0063 | 0.0038 | 0.0083 | 0.0079 | 0.0083 | 0.0024 | 0.0009 | 0.0227 | 0.0029 | 0.0029 | 0.0032 | 0.0014 |
Peroxisomes | 0.003 | 0 | 0.0034 | 0.0001 | 0 | 0.0007 | 0.009 | 0 | 0.0013 | 0.0103 | 0.0002 | 0.0017 | 0 | 0 | 0.0007 | 0 | 0 | 0 |
Punctate Nuclear | 0.0159 | 0.0002 | 0.0086 | 0.0014 | 0.0005 | 0.0022 | 0.0036 | 0.0003 | 0.0031 | 0.0023 | 0.0153 | 0.0009 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0.0007 | 0.0003 |
Vacuole | 0.001 | 0.0002 | 0.0004 | 0.0008 | 0.0006 | 0.0017 | 0.0042 | 0.0007 | 0.0015 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0181 | 0.0204 | 0.0277 | 0.0305 | 0.0325 | 0.0131 | 0.0269 | 0.0503 | 0.0325 | 0.0399 | 0.0363 | 0.0145 | 0.027 | 0.0417 | 0.0355 | 0.039 | 0.0243 | 0.015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5221 | 26.2481 | 18.4521 | 20.097 | 20.5375 | 8.4949 | 21.476 | 19.5621 | 19.5614 | 23.6211 |
Translational Efficiency | 3.1437 | 2.1526 | 2.0273 | 1.5186 | 2.2884 | 4.4509 | 1.9371 | 1.8566 | 1.7681 | 1.7386 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
868 | 1525 | 735 | 106 | 1838 | 1027 | 149 | 1741 | 2706 | 2552 | 884 | 1847 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1049.84 | 1028.13 | 1092.03 | 1532.71 | 902.90 | 1062.95 | 1340.61 | 1250.45 | 950.03 | 1042.14 | 1133.93 | 1266.65 |
Standard Deviation | 138.34 | 215.26 | 210.44 | 239.10 | 143.86 | 162.83 | 153.20 | 210.39 | 157.80 | 196.60 | 222.34 | 222.07 |
Intensity Change Log 2 | — | -0.030147 | 0.056843 | 0.545915 | — | 0.235436 | 0.570251 | 0.469809 | — | 0.098748 | 0.317023 | 0.511224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000461 | 0.000664 | 0.000629 | 0.000090 | 0.000628 | 0.000688 | 0.000489 | 0.000525 | 0.000574 | 0.000674 | 0.000606 | 0.000500 |
Bud Neck | 0.000654 | 0.000796 | 0.001615 | 0.002077 | 0.000612 | 0.000626 | 0.001361 | 0.002339 | 0.000626 | 0.000728 | 0.001572 | 0.002324 |
Bud Site | 0.000371 | 0.000302 | 0.000194 | 0.000386 | 0.000529 | 0.000193 | 0.000216 | 0.000761 | 0.000478 | 0.000258 | 0.000198 | 0.000739 |
Cell Periphery | 0.000063 | 0.000133 | 0.000040 | 0.000019 | 0.000165 | 0.000155 | 0.000016 | 0.000072 | 0.000132 | 0.000142 | 0.000036 | 0.000069 |
Cytoplasm | 0.000418 | 0.001160 | 0.001694 | 0.000067 | 0.002096 | 0.001236 | 0.000145 | 0.002692 | 0.001558 | 0.001191 | 0.001433 | 0.002541 |
Cytoplasmic Foci | 0.001718 | 0.001867 | 0.000259 | 0.010264 | 0.001311 | 0.001236 | 0.000276 | 0.002333 | 0.001442 | 0.001613 | 0.000262 | 0.002788 |
Eisosomes | 0.000005 | 0.000009 | 0.000006 | 0.000005 | 0.000013 | 0.000012 | 0.000007 | 0.000009 | 0.000010 | 0.000010 | 0.000007 | 0.000008 |
Endoplasmic Reticulum | 0.005281 | 0.007992 | 0.004192 | 0.000571 | 0.012550 | 0.009717 | 0.005858 | 0.002964 | 0.010218 | 0.008686 | 0.004473 | 0.002827 |
Endosome | 0.002728 | 0.002905 | 0.000646 | 0.001329 | 0.003572 | 0.002523 | 0.000431 | 0.001693 | 0.003301 | 0.002751 | 0.000610 | 0.001672 |
Golgi | 0.001048 | 0.001384 | 0.000298 | 0.000609 | 0.001290 | 0.000723 | 0.000196 | 0.000972 | 0.001213 | 0.001118 | 0.000281 | 0.000951 |
Lipid Particles | 0.001381 | 0.004398 | 0.000296 | 0.003107 | 0.012109 | 0.005501 | 0.000501 | 0.001020 | 0.008668 | 0.004842 | 0.000331 | 0.001139 |
Mitochondria | 0.006391 | 0.007995 | 0.002519 | 0.000950 | 0.014356 | 0.009307 | 0.001324 | 0.004589 | 0.011801 | 0.008523 | 0.002317 | 0.004380 |
Mitotic Spindle | 0.007539 | 0.003960 | 0.011211 | 0.030721 | 0.001087 | 0.002159 | 0.011034 | 0.023084 | 0.003157 | 0.003235 | 0.011181 | 0.023522 |
None | 0.000378 | 0.003134 | 0.001113 | 0.000075 | 0.004295 | 0.002508 | 0.000180 | 0.002701 | 0.003039 | 0.002882 | 0.000956 | 0.002551 |
Nuclear Periphery | 0.671354* | 0.517608* | 0.488807* | 0.674004* | 0.502287* | 0.524613* | 0.600593* | 0.589731* | 0.556518* | 0.520427* | 0.507649* | 0.594568* |
Nuclear Periphery Foci | 0.242316* | 0.368997* | 0.322106* | 0.239472* | 0.379236* | 0.339433* | 0.288913* | 0.260596* | 0.335316* | 0.357100* | 0.316511* | 0.259383* |
Nucleolus | 0.015902 | 0.022376 | 0.053111 | 0.007107 | 0.017773 | 0.033289 | 0.036172 | 0.031406 | 0.017173 | 0.026767 | 0.050256 | 0.030011 |
Nucleus | 0.029800 | 0.031463 | 0.105718 | 0.003201 | 0.016777 | 0.047891 | 0.048749 | 0.056660 | 0.020955 | 0.038074 | 0.096116 | 0.053592 |
Peroxisomes | 0.000477 | 0.000505 | 0.000497 | 0.000399 | 0.000682 | 0.000770 | 0.000767 | 0.000636 | 0.000616 | 0.000612 | 0.000543 | 0.000623 |
Vacuole | 0.004999 | 0.007927 | 0.001638 | 0.008559 | 0.009496 | 0.006862 | 0.001248 | 0.005790 | 0.008054 | 0.007498 | 0.001572 | 0.005949 |
Vacuole Periphery | 0.006715 | 0.014424 | 0.003410 | 0.016988 | 0.019136 | 0.010559 | 0.001523 | 0.009426 | 0.015152 | 0.012869 | 0.003092 | 0.009860 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.07 | -2.19 | 4.53 | 8.74 | 7.94 | -0.55 | 1.11 | 0.60 | 1.19 | -0.74 | -1.35 | -1.03 | 0.37 | 1.86 | 1.43 |
Bud Neck | -0.84 | -5.50 | -1.83 | -1.71 | -0.67 | -0.21 | -1.03 | -6.90 | -6.69 | -0.82 | -1.22 | -4.70 | -7.07 | -6.75 | -2.65 |
Bud Site | 0.38 | 0.13 | -0.14 | -0.31 | -0.21 | 1.82 | 0.73 | -1.29 | -3.13 | -1.74 | 1.56 | 0.79 | -1.59 | -2.82 | -2.18 |
Cell Periphery | -2.55 | 0.93 | 2.07 | 6.38 | 3.34 | 0.12 | 9.36 | 5.02 | 1.27 | -6.80 | -0.36 | 6.90 | 4.01 | 2.45 | -3.67 |
Cytoplasm | -2.97 | -2.76 | 3.46 | 4.68 | 3.41 | 1.17 | 3.71 | -1.40 | -2.53 | -4.79 | 0.70 | -0.34 | -2.14 | -2.78 | -1.66 |
Cytoplasmic Foci | -0.24 | 2.10 | -1.19 | -1.17 | -1.38 | 0.21 | 3.89 | -1.62 | -1.63 | -3.52 | -0.42 | 4.03 | -1.86 | -1.60 | -3.60 |
Eisosomes | -3.22 | -1.61 | 0.15 | 1.97 | 1.19 | 0.53 | 2.29 | 2.37 | 1.54 | -0.81 | 0.04 | 2.09 | 0.90 | 0.90 | -1.11 |
Endoplasmic Reticulum | -2.40 | 1.05 | 4.86 | 14.30 | 11.81 | 2.30 | 4.98 | 11.50 | 6.65 | 2.50 | 1.96 | 8.24 | 10.92 | 10.37 | 3.91 |
Endosome | -0.22 | 2.74 | 1.45 | 2.21 | -0.99 | 1.84 | 8.90 | 4.33 | 1.43 | -3.80 | 1.29 | 8.06 | 3.79 | 2.65 | -3.62 |
Golgi | -0.77 | 1.86 | 0.94 | 2.16 | -1.22 | 1.35 | 3.87 | 0.90 | -0.69 | -3.38 | 0.28 | 4.13 | 0.82 | 0.53 | -3.31 |
Lipid Particles | -3.56 | 1.85 | -1.18 | 0.89 | -2.07 | 4.36 | 9.95 | 9.38 | 4.50 | -2.52 | 3.90 | 10.17 | 8.93 | 6.36 | -3.94 |
Mitochondria | -0.79 | 2.02 | 2.89 | 8.01 | 3.41 | 2.99 | 9.86 | 6.72 | 3.72 | -5.22 | 2.60 | 8.45 | 6.02 | 4.60 | -3.24 |
Mitotic Spindle | 1.89 | -1.05 | -2.28 | -2.67 | -1.96 | -1.48 | -1.95 | -9.03 | -8.43 | -2.44 | 0.08 | -3.35 | -8.37 | -8.44 | -4.04 |
None | -4.60 | -3.04 | 2.77 | 5.12 | 4.39 | 2.18 | 5.92 | 1.56 | -0.38 | -3.93 | 0.17 | 3.91 | 0.50 | 0.38 | -2.46 |
Nuclear Periphery | 13.70* | 13.25 | 0.09 | -5.59 | -6.48 | -2.13 | -4.25 | -8.11 | -4.98 | 0.79 | 4.80 | 4.65 | -3.28 | -7.26 | -6.64 |
Nuclear Periphery Foci | -14.87* | -7.53 | 0.27 | 7.16 | 4.30 | 4.54 | 5.44 | 16.22 | 9.47 | 1.81 | -3.48 | 2.38 | 11.91 | 15.04 | 6.52 |
Nucleolus | -2.32 | -8.24 | 1.13 | 2.42 | 6.99 | -4.83 | -2.53 | -5.63 | -0.24 | 0.38 | -4.84 | -8.84 | -5.82 | -2.11 | 4.16 |
Nucleus | -0.14 | -8.75 | 5.73 | 7.82 | 12.59 | -6.70 | -3.15 | -10.32 | -2.33 | -1.07 | -5.83 | -10.95 | -8.88 | -4.52 | 5.07 |
Peroxisomes | -0.20 | -0.43 | 0.12 | 0.23 | 0.35 | -0.96 | -0.73 | -0.05 | 0.48 | 0.52 | 0.03 | 0.29 | -0.38 | -0.41 | -0.54 |
Vacuole | -1.91 | 2.38 | -0.93 | -0.21 | -1.81 | 1.82 | 7.86 | 2.95 | 0.46 | -4.48 | 0.56 | 8.58 | 1.66 | 1.10 | -5.32 |
Vacuole Periphery | -3.93 | 2.07 | -1.64 | -0.42 | -2.22 | 4.79 | 13.60 | 6.20 | 0.73 | -7.50 | 1.70 | 11.20 | 3.86 | 2.28 | -6.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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