Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 4 | 3 | 2 | 5 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 3 | 8 | 1 | 0 | 2 | 2 | 16 | 14 | 2 | 3 | 11 | 9 |
Bud | 10 | 7 | 4 | 4 | 8 | 7 | 5 | 8 | 31 | 17 | 17 | 19 | 1 | 2 | 2 | 12 | 6 | 4 | 1 | 1 | 0 | 2 | 2 | 2 |
Bud Neck | 0 | 0 | 1 | 0 | 4 | 1 | 3 | 2 | 2 | 2 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 6 | 5 | 1 | 1 | 2 | 3 |
Bud Site | 2 | 1 | 0 | 1 | 0 | 0 | 4 | 2 | 10 | 2 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 16 | 24 | 6 | 7 | 1 | 5 | 3 | 4 | 5 | 2 | 4 | 4 | 7 | 1 | 3 | 26 | 44 | 38 | 1 | 2 | 0 | 0 | 0 | 1 |
Cytoplasm | 15 | 42 | 12 | 18 | 23 | 11 | 14 | 14 | 17 | 16 | 12 | 14 | 25 | 19 | 47 | 40 | 64 | 55 | 1 | 4 | 1 | 2 | 4 | 3 |
Endoplasmic Reticulum | 22 | 12 | 7 | 2 | 12 | 5 | 9 | 4 | 8 | 2 | 2 | 4 | 4 | 5 | 12 | 9 | 11 | 14 | 2 | 2 | 1 | 1 | 2 | 5 |
Endosome | 28 | 10 | 23 | 32 | 3 | 35 | 22 | 17 | 2 | 26 | 7 | 1 | 22 | 10 | 51 | 11 | 24 | 8 | 74 | 36 | 26 | 19 | 27 | 24 |
Golgi | 8 | 11 | 7 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 7 | 9 | 1 | 11 | 56 | 70 | 29 | 22 | 8 | 8 | 18 | 12 |
Mitochondria | 508 | 311 | 94 | 149 | 163 | 195 | 264 | 206 | 589 | 472 | 441 | 474 | 60 | 19 | 63 | 25 | 35 | 21 | 545 | 365 | 215 | 204 | 253 | 257 |
Nucleus | 7 | 2 | 3 | 3 | 7 | 10 | 21 | 10 | 10 | 10 | 9 | 11 | 1 | 3 | 0 | 8 | 6 | 9 | 1 | 2 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 6 | 1 | 1 | 1 | 0 | 1 | 7 | 7 | 13 | 14 | 9 | 7 | 0 | 0 | 1 | 1 | 3 | 0 | 7 | 2 | 1 | 1 | 1 | 3 |
Nucleolus | 4 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 |
Peroxisomes | 9 | 9 | 16 | 15 | 0 | 3 | 0 | 3 | 2 | 10 | 4 | 1 | 5 | 4 | 37 | 31 | 44 | 14 | 25 | 8 | 26 | 12 | 23 | 12 |
SpindlePole | 23 | 7 | 31 | 8 | 18 | 19 | 51 | 32 | 24 | 35 | 20 | 27 | 6 | 4 | 9 | 12 | 17 | 21 | 7 | 3 | 3 | 1 | 4 | 3 |
Vac/Vac Membrane | 73 | 77 | 22 | 23 | 12 | 39 | 50 | 45 | 23 | 32 | 18 | 22 | 19 | 14 | 24 | 29 | 58 | 49 | 19 | 30 | 8 | 12 | 13 | 18 |
Unique Cell Count | 606 | 413 | 188 | 205 | 222 | 272 | 393 | 298 | 620 | 540 | 477 | 509 | 137 | 85 | 206 | 177 | 303 | 254 | 755 | 509 | 302 | 277 | 375 | 368 |
Labelled Cell Count | 741 | 519 | 230 | 270 | 256 | 333 | 455 | 357 | 741 | 647 | 551 | 597 | 160 | 100 | 251 | 216 | 373 | 306 | 755 | 509 | 302 | 277 | 375 | 368 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 4.9 | 5.7 | 5.6 | 4.7 | 5.0 | 5.0 | 5.2 | 4.0 | 4.6 | 4.3 | 4.4 | 6.0 | 6.0 | 5.7 | 6.2 | 7.6 | 8.2 | 6.1 | 6.6 | 6.8 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.8 | 1.2 | 1.3 | 0.7 | 0.9 | 1.0 | 0.7 | 0.8 | 0.7 | 0.6 | 1.0 | 0.9 | 1.3 | 1.6 | 2.1 | 2.3 | 1.1 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.29 | -0.18 | -0.17 | -0.13 | -0.52 | -0.3 | -0.4 | -0.37 | 0.07 | 0.07 | 0.01 | 0.12 | 0.42 | 0.53 | 0.09 | 0.2 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 3.9 | 4.0 | 4.1 |
Cytoplasm | 0.9 | 1.4 | -1.1 | -1.5 | -0.8 | -2.4 | -2.1 | -2.4 | -2.2 | 3.3 | 3.9 | 4.6 | 4.4 | 4.4 | 4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0.6 | -0.1 | 0.9 |
Endosome | 1.0 | 0 | 0.2 | -2.8 | -2.6 | 0 | 0 | 0 | 0 | 1.0 | -0.1 | 3.2 | -2.0 | -1.6 | -3.7 |
Golgi | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 1.1 | 4.8 | 6.5 |
Mitochondria | 4.6 | 0 | 4.7 | 4.0 | 4.2 | 0 | 0 | 0 | 0 | -1.1 | -4.3 | -3.9 | -7.3 | -9.4 | -9.9 |
Nucleus | 0 | 0 | 1.3 | 2.1 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.4 | 0 | -3.9 | -5.9 | -4.2 | 0 | 0 | 0 | 0 | -1.8 | -1.1 | 2.7 | 2.6 | 2.0 | -1.2 |
SpindlePole | -4.2 | 0 | -3.2 | -1.1 | -1.8 | 0 | 0 | 0 | 0 | -3.4 | -2.7 | -4.0 | -2.9 | -3.9 | -2.7 |
Vacuole | -0.2 | -2.3 | 0.8 | 0.3 | 1.1 | -4.2 | -2.6 | -3.9 | -3.6 | 0.6 | 1.1 | 0 | 1.3 | 2.2 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3329 | 1.4884 | 1.3286 | 1.2828 | 1.2475 | 1.5676 | 6.7998 | 6.9783 | 6.8611 | 6.9442 | 7.351 | 7.4163 | 3.8654 | 3.7187 | 4.0806 | 4.2566 | 3.7998 | 4.2022 |
Actin | 0.0322 | 0.014 | 0.008 | 0.0283 | 0.0227 | 0.0101 | 0.0318 | 0.0167 | 0.021 | 0.0205 | 0.0418 | 0.0221 | 0.0133 | 0.007 | 0.0145 | 0.0046 | 0.0035 | 0.0067 |
Bud | 0.0499 | 0.0581 | 0.0815 | 0.0806 | 0.0301 | 0.0513 | 0.0021 | 0.0029 | 0.0023 | 0.0018 | 0.0014 | 0.0009 | 0.0006 | 0.0038 | 0.0016 | 0.0012 | 0.0008 | 0.0015 |
Bud Neck | 0.0062 | 0.0014 | 0.0019 | 0.0071 | 0.002 | 0.0058 | 0.003 | 0.0009 | 0.0033 | 0.0014 | 0.001 | 0.0022 | 0.0011 | 0.001 | 0.0015 | 0.0008 | 0.0005 | 0.0036 |
Bud Periphery | 0.0191 | 0.0169 | 0.0241 | 0.0406 | 0.0074 | 0.0233 | 0.0055 | 0.0048 | 0.0063 | 0.006 | 0.0068 | 0.0022 | 0.0014 | 0.0033 | 0.0043 | 0.0021 | 0.0036 | 0.0037 |
Bud Site | 0.0328 | 0.091 | 0.051 | 0.0422 | 0.0129 | 0.0066 | 0.0053 | 0.0246 | 0.0089 | 0.0033 | 0.002 | 0.0013 | 0.0029 | 0.0182 | 0.0058 | 0.0023 | 0.0012 | 0.003 |
Cell Periphery | 0.0026 | 0.0021 | 0.0029 | 0.0031 | 0.0009 | 0.0026 | 0.0014 | 0.0007 | 0.001 | 0.001 | 0.0046 | 0.0006 | 0.0016 | 0.001 | 0.0018 | 0.0004 | 0.0003 | 0.0012 |
Cytoplasm | 0.0292 | 0.0259 | 0.0419 | 0.0238 | 0.0571 | 0.1022 | 0.0093 | 0.0119 | 0.0079 | 0.0237 | 0.0052 | 0.0047 | 0.0041 | 0.0098 | 0.0138 | 0.0055 | 0.0044 | 0.009 |
Cytoplasmic Foci | 0.0807 | 0.0764 | 0.0358 | 0.0509 | 0.0734 | 0.0861 | 0.0476 | 0.0629 | 0.0387 | 0.0456 | 0.0273 | 0.0474 | 0.0463 | 0.0457 | 0.0477 | 0.0357 | 0.0392 | 0.052 |
Eisosomes | 0.0008 | 0.0005 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0012 | 0.0004 | 0.0005 | 0.0016 | 0.0022 | 0.0004 | 0.0006 | 0.0003 | 0.0008 | 0.0006 | 0.0003 | 0.0005 |
Endoplasmic Reticulum | 0.0028 | 0.0014 | 0.0011 | 0.0014 | 0.0008 | 0.0016 | 0.0026 | 0.003 | 0.0014 | 0.0053 | 0.0034 | 0.0011 | 0.0018 | 0.0017 | 0.0015 | 0.0004 | 0.0008 | 0.0016 |
Endosome | 0.0937 | 0.1003 | 0.0537 | 0.0736 | 0.1312 | 0.0482 | 0.1236 | 0.1186 | 0.0698 | 0.1044 | 0.118 | 0.0953 | 0.1296 | 0.1334 | 0.0858 | 0.0523 | 0.0549 | 0.0936 |
Golgi | 0.0659 | 0.0771 | 0.0327 | 0.034 | 0.0698 | 0.0463 | 0.1037 | 0.1076 | 0.075 | 0.1115 | 0.1605 | 0.0991 | 0.0991 | 0.1139 | 0.0823 | 0.0487 | 0.0704 | 0.1092 |
Lipid Particles | 0.0332 | 0.0095 | 0.0062 | 0.0324 | 0.0057 | 0.0253 | 0.0328 | 0.0086 | 0.0146 | 0.0212 | 0.0066 | 0.0119 | 0.0345 | 0.0116 | 0.0133 | 0.0098 | 0.014 | 0.0195 |
Mitochondria | 0.4289 | 0.4279 | 0.5492 | 0.4489 | 0.4741 | 0.4453 | 0.5461 | 0.5603 | 0.6832 | 0.5656 | 0.5638 | 0.6503 | 0.5987 | 0.5937 | 0.6562 | 0.748 | 0.7081 | 0.6207 |
None | 0.0391 | 0.0074 | 0.0212 | 0.0411 | 0.0143 | 0.0385 | 0.0128 | 0.0155 | 0.0139 | 0.0358 | 0.0039 | 0.0056 | 0.0116 | 0.0036 | 0.0115 | 0.0205 | 0.0032 | 0.0116 |
Nuclear Periphery | 0.0046 | 0.001 | 0.0018 | 0.0022 | 0.0015 | 0.0042 | 0.003 | 0.0021 | 0.0017 | 0.0016 | 0.0007 | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0007 |
Nucleolus | 0.0053 | 0.0026 | 0.0052 | 0.007 | 0.0038 | 0.0063 | 0.0005 | 0.0005 | 0.0008 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0003 | 0.0006 |
Nucleus | 0.0057 | 0.006 | 0.0216 | 0.0212 | 0.0317 | 0.0237 | 0.0014 | 0.0006 | 0.0008 | 0.0009 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0007 |
Peroxisomes | 0.0508 | 0.0611 | 0.0474 | 0.0363 | 0.03 | 0.0486 | 0.0507 | 0.047 | 0.0396 | 0.0361 | 0.0427 | 0.0464 | 0.0425 | 0.0385 | 0.0472 | 0.0628 | 0.0888 | 0.0498 |
Punctate Nuclear | 0.0059 | 0.0089 | 0.0039 | 0.0084 | 0.0187 | 0.0137 | 0.0008 | 0.0009 | 0.0011 | 0.0012 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0007 |
Vacuole | 0.0076 | 0.0071 | 0.0063 | 0.0114 | 0.0081 | 0.0069 | 0.0097 | 0.0057 | 0.005 | 0.0064 | 0.004 | 0.0037 | 0.0055 | 0.008 | 0.0058 | 0.0017 | 0.0025 | 0.0059 |
Vacuole Periphery | 0.0032 | 0.0033 | 0.0021 | 0.005 | 0.0036 | 0.0033 | 0.005 | 0.0038 | 0.0029 | 0.0044 | 0.0034 | 0.0031 | 0.0035 | 0.0041 | 0.003 | 0.0018 | 0.0027 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0571 | 10.2748 | 10.4834 | 15.5538 | 13.0394 | 24.4108 | 20.4529 | 24.1028 | 21.5029 | 19.8597 |
Translational Efficiency | 1.4531 | 0.9315 | 1.1484 | 0.8885 | 0.7763 | 0.8771 | 0.916 | 0.6277 | 0.9063 | 1.1304 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
202 | 1164 | 1957 | 1510 | 1810 | 1826 | 1893 | 1687 | 2012 | 2990 | 3850 | 3197 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 590.26 | 776.06 | 845.70 | 797.02 | 701.36 | 857.03 | 822.81 | 820.64 | 690.21 | 825.51 | 834.45 | 809.48 |
Standard Deviation | 81.58 | 106.41 | 108.18 | 111.55 | 88.64 | 119.96 | 114.61 | 189.19 | 94.08 | 121.47 | 111.97 | 157.81 |
Intensity Change Log 2 | — | 0.394818 | 0.518795 | 0.433265 | — | 0.289191 | 0.230404 | 0.226594 | — | 0.338423 | 0.369379 | 0.324727 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000561 | 0.004807 | 0.005662 | 0.018107 | 0.001248 | 0.002260 | 0.007972 | 0.005186 | 0.001179 | 0.003251 | 0.006798 | 0.011289 |
Bud Neck | 0.012478 | 0.006111 | 0.009968 | 0.021212 | 0.007269 | 0.009657 | 0.027264 | 0.019488 | 0.007792 | 0.008276 | 0.018472 | 0.020303 |
Bud Site | 0.010159 | 0.024775 | 0.056816 | 0.080951 | 0.008346 | 0.012363 | 0.059919 | 0.083651 | 0.008528 | 0.017195 | 0.058342 | 0.082376 |
Cell Periphery | 0.001398 | 0.000471 | 0.000241 | 0.000511 | 0.000343 | 0.000550 | 0.000642 | 0.000960 | 0.000449 | 0.000519 | 0.000438 | 0.000748 |
Cytoplasm | 0.003986 | 0.007362 | 0.014238 | 0.038948 | 0.009981 | 0.007738 | 0.029183 | 0.035522 | 0.009379 | 0.007592 | 0.021586 | 0.037140 |
Cytoplasmic Foci | 0.057518 | 0.062450 | 0.056733 | 0.098692 | 0.089666 | 0.070773 | 0.091041 | 0.067282 | 0.086438 | 0.067533 | 0.073602 | 0.082117 |
Eisosomes | 0.000107 | 0.000138 | 0.000096 | 0.000224 | 0.000161 | 0.000172 | 0.000306 | 0.000152 | 0.000156 | 0.000159 | 0.000200 | 0.000186 |
Endoplasmic Reticulum | 0.000866 | 0.000513 | 0.001299 | 0.004396 | 0.000811 | 0.000356 | 0.003391 | 0.001578 | 0.000816 | 0.000417 | 0.002328 | 0.002909 |
Endosome | 0.043223 | 0.100929 | 0.087341 | 0.093124 | 0.064598 | 0.090085 | 0.110186 | 0.103593 | 0.062452 | 0.094306 | 0.098574 | 0.098648 |
Golgi | 0.085991 | 0.167295* | 0.095701 | 0.083223 | 0.192132* | 0.159867* | 0.089459 | 0.093132 | 0.181476* | 0.162759* | 0.092632 | 0.088452 |
Lipid Particles | 0.008937 | 0.001253 | 0.000712 | 0.010078 | 0.008866 | 0.005240 | 0.011906 | 0.009294 | 0.008873 | 0.003688 | 0.006216 | 0.009665 |
Mitochondria | 0.700713* | 0.528417* | 0.462096* | 0.265906* | 0.548603* | 0.542857* | 0.320705* | 0.388538* | 0.563874* | 0.537235* | 0.392576* | 0.330617* |
Mitotic Spindle | 0.009312 | 0.013816 | 0.077810 | 0.058294 | 0.007653 | 0.011222 | 0.072604 | 0.044430 | 0.007820 | 0.012232 | 0.075250 | 0.050978 |
None | 0.000324 | 0.000201 | 0.000233 | 0.000999 | 0.000857 | 0.000846 | 0.001553 | 0.001596 | 0.000803 | 0.000595 | 0.000882 | 0.001314 |
Nuclear Periphery | 0.000179 | 0.000124 | 0.000426 | 0.001637 | 0.000209 | 0.000084 | 0.001143 | 0.000641 | 0.000206 | 0.000099 | 0.000779 | 0.001111 |
Nuclear Periphery Foci | 0.000322 | 0.000332 | 0.000376 | 0.002186 | 0.000417 | 0.000213 | 0.001263 | 0.001657 | 0.000408 | 0.000259 | 0.000812 | 0.001907 |
Nucleolus | 0.001891 | 0.000333 | 0.000733 | 0.002621 | 0.000693 | 0.001354 | 0.003100 | 0.002840 | 0.000814 | 0.000957 | 0.001896 | 0.002736 |
Nucleus | 0.003472 | 0.000782 | 0.002135 | 0.013553 | 0.001059 | 0.001845 | 0.012701 | 0.007262 | 0.001301 | 0.001431 | 0.007330 | 0.010233 |
Peroxisomes | 0.043950 | 0.049068 | 0.043854 | 0.075660 | 0.030726 | 0.047416 | 0.049077 | 0.049312 | 0.032054 | 0.048059 | 0.046422 | 0.061757 |
Vacuole | 0.005391 | 0.016854 | 0.061338 | 0.106652 | 0.010629 | 0.020738 | 0.084045 | 0.059125 | 0.010103 | 0.019226 | 0.072503 | 0.081573 |
Vacuole Periphery | 0.009220 | 0.013971 | 0.022191 | 0.023026 | 0.015734 | 0.014364 | 0.022541 | 0.024762 | 0.015080 | 0.014211 | 0.022363 | 0.023942 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.83 | -7.35 | -8.93 | -6.20 | -5.99 | -2.30 | -6.80 | -5.35 | -3.56 | 2.29 | -4.69 | -9.28 | -9.97 | -7.38 | -3.81 |
Bud Neck | 1.75 | 0.56 | -2.39 | -8.88 | -6.16 | -1.48 | -8.59 | -6.09 | -4.92 | 2.90 | -0.37 | -7.02 | -8.07 | -8.91 | -1.20 |
Bud Site | -3.64 | -11.13 | -14.06 | -12.67 | -5.07 | -3.08 | -18.06 | -18.08 | -16.77 | -4.76 | -6.54 | -24.47 | -25.44 | -21.53 | -6.94 |
Cell Periphery | 1.02 | 1.27 | 0.98 | -0.51 | -6.82 | -1.19 | -2.58 | -2.84 | -1.49 | -1.35 | -0.49 | 0.13 | -1.97 | -1.44 | -2.46 |
Cytoplasm | -1.62 | -5.37 | -11.98 | -12.17 | -9.65 | 1.85 | -9.79 | -9.76 | -11.09 | -2.10 | 1.62 | -9.28 | -14.42 | -16.50 | -7.94 |
Cytoplasmic Foci | -0.49 | 0.30 | -5.16 | -8.88 | -11.09 | 5.38 | -0.50 | 6.01 | 0.81 | 6.38 | 6.33 | 4.28 | 1.21 | -5.66 | -3.35 |
Eisosomes | -2.23 | 1.07 | -6.28 | -4.82 | -8.03 | -0.74 | -2.68 | 0.62 | 1.34 | 2.85 | -0.24 | -1.57 | -2.36 | -2.32 | 0.49 |
Endoplasmic Reticulum | 0.83 | -1.16 | -4.49 | -5.78 | -4.40 | 3.32 | -4.66 | -3.56 | -6.47 | 3.14 | 3.11 | -5.15 | -5.99 | -7.50 | -1.27 |
Endosome | -7.57 | -6.21 | -6.63 | 1.74 | -1.06 | -6.28 | -10.47 | -8.94 | -2.72 | 1.28 | -9.30 | -11.01 | -10.42 | -0.96 | 0.09 |
Golgi | -7.01 | -0.85 | 0.43 | 12.68 | 2.77 | 4.56 | 16.81 | 15.72 | 11.47 | -0.88 | 3.05 | 16.54 | 17.13 | 17.08 | 1.29 |
Lipid Particles | 3.51 | 3.73 | -0.55 | -6.89 | -7.28 | 2.68 | -1.88 | -0.33 | -2.92 | 1.53 | 4.65 | 2.13 | -0.74 | -6.11 | -3.21 |
Mitochondria | 6.62 | 9.69 | 17.72 | 21.02 | 18.08* | 0.51 | 21.48 | 14.24 | 13.69 | -6.14 | 2.67 | 18.71 | 24.86 | 24.68 | 7.87 |
Mitotic Spindle | -1.14 | -13.39 | -9.25 | -10.98 | 3.75 | -2.31 | -16.58 | -11.93 | -10.74 | 5.83 | -3.28 | -24.27 | -16.80 | -15.44 | 6.86 |
None | 1.38 | 1.17 | -2.05 | -3.34 | -3.20 | 0.02 | -1.19 | -1.48 | -1.79 | -0.17 | 0.49 | -0.15 | -1.23 | -2.72 | -1.83 |
Nuclear Periphery | 0.49 | -2.05 | -4.05 | -4.29 | -3.32 | 1.48 | -3.28 | -2.76 | -4.13 | 1.65 | 1.34 | -3.64 | -4.69 | -5.61 | -1.39 |
Nuclear Periphery Foci | -0.01 | -0.66 | -3.32 | -3.23 | -3.14 | 1.61 | -2.26 | -3.14 | -3.74 | -0.82 | 1.12 | -2.09 | -4.42 | -4.92 | -2.86 |
Nucleolus | 1.17 | 1.04 | 0.36 | -3.36 | -2.82 | -1.27 | -3.56 | -3.28 | -2.54 | -0.13 | -0.03 | -1.73 | -3.07 | -3.90 | -1.92 |
Nucleus | 1.53 | 0.52 | -4.56 | -7.96 | -6.84 | -1.79 | -8.86 | -6.32 | -5.19 | 3.39 | -0.36 | -8.67 | -9.59 | -9.36 | -2.68 |
Peroxisomes | -0.64 | 0.02 | -3.81 | -4.70 | -6.51 | -4.84 | -5.50 | -5.28 | -0.44 | -0.03 | -5.39 | -5.35 | -9.39 | -4.00 | -4.87 |
Vacuole | -5.79 | -18.93 | -21.52 | -19.37 | -8.86 | -6.01 | -21.24 | -17.43 | -12.77 | 5.95 | -7.16 | -27.88 | -26.69 | -22.68 | -2.75 |
Vacuole Periphery | -2.04 | -5.53 | -5.51 | -4.71 | -0.25 | 1.02 | -4.19 | -5.51 | -6.22 | -1.31 | 0.76 | -5.85 | -6.72 | -7.75 | -1.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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