Standard name
Description Protein involved in mitochondrial fission and peroxisome abundance; may have a distinct role in tethering protein aggregates to mitochondria in order to retain them in the mother cell; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.92 0.93 0.89 0.96 0.93 0.88 0.81 0.77 – 0.7 0.58 0.58 0.97 0.96 0.96 0.93 0.96 0.87 0.91 0.92 0.84 0.73 0.79 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0 0.14 0.2 0.19 – 0.28 0.41 0.41 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 2 0 0 2 – 1 0 1 0 0 2 3 0 1 2 1 0 0 1 6
Bud 1 1 1 4 10 22 15 17 – 17 26 19 3 0 2 2 1 1 0 0 1 0 3 4
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 3 2 2 0 2 2
Bud Site 0 0 0 0 0 2 4 2 – 2 5 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 3 0 7 8 9 15 6 – 18 11 7 3 6 14 5 6 7 0 0 0 0 0 0
Cytoplasm 136 173 33 251 422 342 435 400 – 329 228 260 168 208 320 122 65 82 152 185 58 36 146 163
Endoplasmic Reticulum 2 8 1 5 7 2 12 3 – 13 2 8 2 3 8 7 3 2 0 0 2 1 1 5
Endosome 2 1 0 0 3 1 4 1 – 7 3 2 1 1 1 0 1 2 0 1 0 1 7 19
Golgi 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 2 0 1 0 0 0 1
Mitochondria 0 0 2 3 14 53 107 98 – 133 160 185 0 0 0 1 0 1 0 0 0 2 2 8
Nucleus 0 2 0 2 1 4 10 15 – 19 15 21 1 0 2 1 0 0 1 0 0 0 3 0
Nuclear Periphery 1 0 0 0 1 0 1 1 – 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 2 1 0 2 – 1 1 3 0 2 0 1 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 2 – 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 1 1 3 6 – 5 3 7 0 2 1 3 1 0 1 0 0 2 5 4
Vac/Vac Membrane 8 1 0 0 10 4 11 20 – 17 17 5 2 5 3 2 0 6 1 2 0 1 6 8
Unique Cell Count 148 187 37 261 453 390 540 517 470 394 447 174 217 335 131 68 94 168 201 70 50 184 231
Labelled Cell Count 155 192 37 272 481 441 617 575 562 472 526 180 227 354 148 78 105 168 201 70 50 184 231


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.0 5.6 6.1 5.7 5.2 4.4 4.8 – 4.6 4.5 4.8 8.1 7.9 7.9 8.9 9.3 9.5 – 8.9 9.0
Std Deviation (1e-4) 1.0 1.0 0.8 1.0 1.2 1.3 0.9 1.4 – 1.1 0.7 1.0 1.1 1.0 1.0 1.3 1.5 1.4 – 1.6 1.7
Intensity Change (Log2) – – – 0.13 0.02 -0.1 -0.36 -0.21 – -0.29 -0.32 -0.21 0.54 0.5 0.5 0.67 0.73 0.76 – 0.67 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.9 -0.3 -1.3 -1.7 – -2.5 -3.7 -3.7 1.9 1.7 1.7 0.8 1.3 -0.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6681 6.8716 5.8692 6.4008 5.1232 6.1072 5.8249 7.1152 6.2232 4.8205 5.1715 6.3585 4.786 5.7051 4.8801 5.0318 4.0813 4.8859
Actin 0.0083 0.0039 0.0183 0.0369 0.0247 0.0163 0.0092 0.0014 0.0135 0.0037 0.0083 0.0038 0.0007 0.0012 0.0065 0.0005 0.0005 0.0008
Bud 0.0013 0.0024 0.0025 0.0007 0.0007 0.0009 0.0003 0.0012 0.0011 0.0027 0.0002 0.0003 0 0.0067 0.0013 0 0.0003 0.0003
Bud Neck 0.0043 0.0009 0.0214 0.0322 0.0078 0.0089 0.0005 0.0015 0.0259 0.0371 0.0023 0.0083 0.0004 0.0003 0.008 0.0005 0.0023 0.0048
Bud Periphery 0.0023 0.0012 0.0038 0.001 0.001 0.0011 0.0003 0.0021 0.0024 0.003 0.0002 0.0004 0 0.0059 0.0009 0 0.0002 0.0004
Bud Site 0.0058 0.003 0.0339 0.0033 0.0159 0.0119 0.0022 0.0421 0.0075 0.0467 0.0009 0.0008 0.0004 0.0107 0.0129 0.0001 0.0003 0.0003
Cell Periphery 0.0011 0.0006 0.0011 0.0012 0.001 0.0004 0.0005 0.0014 0.0011 0.0018 0.0003 0.0002 0.0006 0.0005 0.0003 0.0001 0.0001 0.0002
Cytoplasm 0.8631 0.9397 0.8202 0.8557 0.8556 0.8992 0.9128 0.891 0.8702 0.7847 0.8832 0.9185 0.9151 0.9501 0.9263 0.9942 0.731 0.9523
Cytoplasmic Foci 0.0117 0.0182 0.0317 0.0132 0.006 0.0043 0.0191 0.0051 0.0065 0.0232 0.0155 0.0188 0.0233 0.0024 0.0072 0.0005 0.0859 0.0024
Eisosomes 0.0001 0 0.0002 0.0001 0.0013 0 0 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0201 0.0037 0.0051 0.0019 0.0065 0.0039 0.0062 0.0023 0.0059 0.0106 0.0035 0.0039 0.0128 0.0025 0.0029 0.0015 0.0042 0.0053
Endosome 0.0085 0.0007 0.0173 0.0147 0.0066 0.0061 0.0156 0.0006 0.0054 0.0095 0.0064 0.0068 0.0183 0.0005 0.0087 0.0001 0.1127 0.014
Golgi 0.0027 0.0004 0.0036 0.0056 0.0029 0.0014 0.003 0.0003 0.0104 0.0036 0.0115 0.0065 0.0021 0.0003 0.0027 0 0.0157 0.0029
Lipid Particles 0.0097 0.0011 0.0042 0.006 0.0023 0.0006 0.0043 0.0176 0.0017 0.0399 0.0072 0.0052 0.004 0.0003 0.0011 0 0.0015 0.0025
Mitochondria 0.0029 0.0002 0.0032 0.0016 0.0032 0.0006 0.0006 0.0001 0.0107 0.0026 0.0077 0.0047 0.0001 0.0002 0.004 0 0.0061 0.001
None 0.0195 0.0202 0.0053 0.0019 0.0441 0.025 0.0089 0.0257 0.0174 0.0052 0.0058 0.0179 0.0173 0.0155 0.0025 0.0016 0.0037 0.0009
Nuclear Periphery 0.0092 0.0004 0.0019 0.0009 0.0025 0.0057 0.0046 0.0002 0.0004 0.001 0.0002 0.0002 0.0004 0.0004 0.0005 0.0002 0.0009 0.0005
Nucleolus 0.0029 0 0.003 0.0012 0.0041 0.0009 0.0004 0.0023 0.0065 0.0064 0.0001 0 0.0006 0.0001 0.0002 0 0.0001 0.0004
Nucleus 0.0035 0.001 0.0031 0.0046 0.0058 0.008 0.0013 0.0011 0.0017 0.0029 0.0005 0.0005 0.0006 0.001 0.0095 0.0005 0.0069 0.001
Peroxisomes 0.0075 0.0009 0.0044 0.0038 0.0012 0.0004 0.004 0.0005 0.0016 0.0062 0.0455 0.0006 0.0005 0.0003 0.0027 0 0.0013 0.0004
Punctate Nuclear 0.0108 0.0011 0.0028 0.0041 0.0049 0.0012 0.005 0.001 0.0004 0.0033 0.0002 0.0019 0.0016 0.0005 0.0002 0 0.0049 0.0001
Vacuole 0.0034 0.0003 0.0104 0.0089 0.0014 0.0028 0.0009 0.0023 0.0082 0.0055 0.0003 0.0005 0.0009 0.0005 0.0011 0.0001 0.0142 0.0077
Vacuole Periphery 0.0012 0 0.0024 0.0006 0.0004 0.0004 0.0005 0.0001 0.0014 0.0005 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0073 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.6906 25.2917 29.7153 34.7383 32.0971 54.1971 27.1707 30.2044 39.0407 39.2337
Translational Efficiency 1.4218 1.9284 1.6483 2.2177 2.1734 1.6879 1.7679 2.5519 1.9507 1.6337

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘