Standard name
Description Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.97 0.83 0.8 0.76 – 0.6 0.58 0.55 0.5 0.56 0.98 0.95 0.94 0.92 0.95 0.91 0.96 0.93 0.92 0.91 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0.07 – 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.2 0.07 0.39 – 0.38 0.57 0.55 0.57 0.61 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.07 0 – 0.08 0.06 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 2 0 1 1 0 2 1 3 0 0 0 2 0 0 0 0 2
Bud 3 0 3 4 7 13 – 34 26 18 12 26 3 2 0 0 0 1 0 0 0 1 4 5
Bud Neck 0 0 0 0 1 1 – 2 0 3 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 1 0 – 1 0 0 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 3 1 0 2 – 2 2 3 2 5 2 2 0 11 21 4 0 0 0 0 0 0
Cytoplasm 504 128 113 175 217 250 – 473 280 278 163 286 319 368 492 218 185 148 502 122 121 149 223 291
Endoplasmic Reticulum 3 0 1 5 0 1 – 1 1 3 2 2 4 3 3 8 4 5 0 0 0 1 0 1
Endosome 0 3 0 4 22 23 – 37 20 14 12 17 3 15 25 5 4 1 2 3 3 0 3 4
Golgi 2 0 0 1 0 0 – 0 0 0 0 0 0 1 0 2 3 3 0 1 0 1 3 3
Mitochondria 6 0 6 42 19 128 – 295 278 279 187 310 1 1 1 5 1 0 4 0 3 2 1 4
Nucleus 1 0 0 0 1 0 – 13 2 6 1 1 0 0 1 1 1 3 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 – 9 2 2 0 5 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 2 2 – 10 1 3 1 4 1 1 0 2 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 3 8 3 – 13 5 4 2 2 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 1 0 0 12 20 14 – 64 28 33 14 27 2 5 9 0 1 3 2 0 0 2 1 7
Vac/Vac Membrane 0 0 0 1 3 5 – 24 9 5 5 7 0 0 3 9 1 7 2 1 0 1 3 2
Unique Cell Count 516 131 116 211 272 331 782 486 510 328 511 327 388 524 237 194 163 525 132 132 163 249 330
Labelled Cell Count 522 132 126 248 302 442 980 654 652 403 698 337 399 537 261 222 175 525 132 132 163 249 330


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 3.6 3.9 4.7 3.9 – 4.0 3.5 3.6 3.4 3.3 5.6 6.2 5.9 10.4 10.3 9.8 4.8 6.0 5.9
Std Deviation (1e-4) 0.6 1.3 0.7 0.9 1.6 1.2 – 1.2 1.2 0.6 0.9 0.8 0.9 1.2 0.9 1.8 1.8 1.7 0.9 1.6 1.1
Intensity Change (Log2) – – – 0.12 0.4 0.12 – 0.15 -0.04 0.0 -0.07 -0.1 0.66 0.8 0.73 1.54 1.53 1.45 0.42 0.75 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 2.6 0
Cytoplasm -3.9 -4.5 -5.2 – -7.8 -8.1 -8.6 -9.1 -8.4 0.1 -1.2 -1.5 -2.0 -0.9 -2.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2781 0.9602 0.5358 0.341 0.5379 0.6554 0.2284 0.696 0.5668 0.4528 0.7433 0.5331 2.2719 2.4863 2.2502 1.6983 2.2066 2.2458
Actin 0.0279 0.002 0.0164 0.0373 0.022 0.0007 0.0156 0.0003 0.0019 0.0039 0.0257 0.01 0.021 0.0023 0.0076 0.0015 0.0035 0.0017
Bud 0.0003 0.0002 0.0007 0.0007 0.0002 0.0001 0.0076 0.0111 0.0082 0.0396 0.0004 0.0007 0.0022 0.0001 0.0019 0.0082 0.0006 0.0011
Bud Neck 0.0004 0.0001 0.0001 0.0049 0.0005 0.0002 0.0007 0.0002 0.0002 0.0012 0.0059 0.0005 0.0021 0.0001 0.0007 0.0007 0.0005 0.0005
Bud Periphery 0.0005 0.0002 0.0012 0.0009 0.0002 0.0001 0.005 0.0023 0.0036 0.019 0.0004 0.0012 0.0027 0.0001 0.0023 0.0127 0.0011 0.0012
Bud Site 0.0022 0.0015 0.0013 0.0066 0.0002 0.0001 0.014 0.0071 0.0032 0.0088 0.0016 0.0009 0.0154 0.0002 0.0036 0.0031 0.0008 0.0004
Cell Periphery 0.0002 0.0003 0.0001 0.0003 0.0001 0 0.0013 0.0003 0.0003 0.0025 0.0002 0.0002 0.0005 0.0001 0.0001 0.0002 0.0001 0
Cytoplasm 0.4588 0.578 0.6069 0.5584 0.5779 0.654 0.4568 0.6093 0.6651 0.4763 0.4898 0.5412 0.2968 0.7003 0.6013 0.6795 0.5701 0.7215
Cytoplasmic Foci 0.0258 0.0059 0.023 0.0403 0.007 0.024 0.0421 0.0064 0.0246 0.0198 0.124 0.0206 0.0802 0.0052 0.0265 0.026 0.0177 0.0167
Eisosomes 0.0006 0.0001 0.0001 0.0002 0.0014 0 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0039 0.0035 0.0033 0.0045 0.0021 0.0017 0.0043 0.0024 0.0032 0.0029 0.0054 0.0072 0.0056 0.0026 0.0023 0.0021 0.0057 0.0028
Endosome 0.0181 0.0016 0.006 0.0336 0.0038 0.0053 0.0322 0.0016 0.0154 0.0064 0.1071 0.0252 0.0467 0.0016 0.0107 0.016 0.0619 0.0191
Golgi 0.0049 0.0002 0.0011 0.0088 0.0041 0.0015 0.014 0.0002 0.004 0.0088 0.0325 0.0104 0.0152 0.0002 0.0051 0.0052 0.0395 0.0027
Lipid Particles 0.0192 0.0002 0.0023 0.0159 0.0177 0.0037 0.0092 0.0002 0.0027 0.0031 0.0496 0.0074 0.0285 0.0009 0.0059 0.0213 0.0122 0.0007
Mitochondria 0.006 0.0001 0.0018 0.0049 0.001 0.0107 0.0167 0.0002 0.0018 0.0013 0.0058 0.0116 0.0257 0.0002 0.0165 0.0025 0.0147 0.0144
None 0.4085 0.4023 0.3218 0.2278 0.3435 0.2909 0.3519 0.3551 0.2523 0.336 0.1306 0.3395 0.393 0.2846 0.2985 0.1644 0.2407 0.206
Nuclear Periphery 0.0016 0.001 0.0027 0.0026 0.0014 0.0007 0.0109 0.0004 0.0009 0.0033 0.0028 0.01 0.0078 0.0003 0.0011 0.0023 0.014 0.0011
Nucleolus 0.0008 0.0001 0.0002 0.0053 0.0003 0.0001 0.0003 0.0001 0.0003 0.0009 0.001 0.0003 0.0022 0 0.0002 0.0016 0.0004 0.0002
Nucleus 0.0012 0.0009 0.0031 0.0036 0.0007 0.0014 0.0013 0.001 0.0015 0.0247 0.0014 0.0018 0.0036 0.0005 0.001 0.0036 0.003 0.0016
Peroxisomes 0.0127 0.0001 0.0025 0.0244 0.0124 0.0008 0.0089 0.0001 0.0071 0.0269 0.0056 0.0062 0.034 0.0001 0.011 0.0252 0.0034 0.0024
Punctate Nuclear 0.0027 0.0004 0.004 0.0049 0.0026 0.0033 0.002 0.0005 0.0017 0.0117 0.0022 0.0011 0.0069 0.0002 0.0016 0.0025 0.0024 0.0014
Vacuole 0.0028 0.0011 0.0012 0.012 0.0007 0.0005 0.003 0.0011 0.0017 0.0025 0.0061 0.0027 0.0077 0.0005 0.0015 0.0167 0.0042 0.0038
Vacuole Periphery 0.0009 0.0001 0.0002 0.002 0.0003 0.0002 0.0019 0.0001 0.0003 0.0003 0.0018 0.0014 0.0019 0.0001 0.0004 0.0046 0.0034 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –