Standard name
Description Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.08 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.96 0.99 0.95 0.92 0.82 0.78 0.78 0.72 0.72 0.69 0.99 1.0 1.0 0.96 0.97 0.98 0.95 0.95 0.93 0.94 0.93 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.05 0.07 0.06 0.14 0.16 0.21 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.1 0 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 8 4 5 4 5 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 3 1 0 0 1 0 3 2 2 4 3 0 0 0 0 0 0 1 0 0 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3 10 6 15 16 24 9 36 1 0 1 7 8 1 0 0 0 0 0 0
Cytoplasm 68 281 132 140 151 250 213 282 200 213 170 262 271 308 358 426 279 210 85 276 126 141 256 225
Endoplasmic Reticulum 1 1 1 0 2 7 9 21 15 24 8 11 0 1 0 14 7 5 0 0 1 0 2 0
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 3 2 2 0 1 1 0 1 0
Golgi 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0 1 0 1
Mitochondria 4 6 6 6 2 14 18 22 35 48 49 90 1 1 0 9 0 0 0 4 1 0 1 6
Nucleus 0 1 0 0 0 0 2 3 1 2 3 2 0 0 0 5 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 1
Vac/Vac Membrane 0 0 1 0 5 4 22 36 11 24 16 22 3 2 1 10 11 2 0 2 1 0 0 4
Unique Cell Count 72 288 137 141 159 272 259 362 257 297 235 382 275 309 358 443 288 214 90 291 136 150 275 259
Labelled Cell Count 74 291 143 147 163 287 275 387 286 344 261 433 280 312 360 475 308 221 90 291 136 150 275 259


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.2 4.7 5.4 11.9 14.0 13.0 14.1 13.6 11.9 12.0 11.7 5.7 5.7 5.9 8.9 10.4 11.8 5.3 5.6 6.5
Std Deviation (1e-4) 1.0 1.0 1.0 1.3 4.2 5.2 4.7 5.0 4.6 4.2 4.6 4.2 1.2 1.3 1.6 3.1 2.7 5.1 1.2 2.1 1.9
Intensity Change (Log2) 0.19 1.34 1.57 1.47 1.59 1.54 1.34 1.36 1.32 0.28 0.28 0.34 0.92 1.15 1.33 0.18 0.25 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 3.4 0 3.7 0 0 0 0 0 0
Cytoplasm 1.7 -0.6 -1.7 -4.0 -4.9 -4.8 -5.9 -5.7 -6.5 1.4 2.8 3.6 -0.1 0.3 1.0
Endoplasmic Reticulum 0 0 0 0 2.5 2.4 3.1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 0 0.3 1.0 0.7 2.9 3.5 4.3 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 3.5 0 3.1 2.7 2.5 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ald2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available