Standard name
Human Ortholog
Description Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.08 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.96 0.99 0.95 0.92 0.82 0.78 0.78 0.72 0.72 0.69 0.99 1.0 1.0 0.96 0.97 0.98 0.95 0.95 0.93 0.94 0.93 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.05 0.07 0.06 0.14 0.16 0.21 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.1 0 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 8 4 5 4 5 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 3 1 0 0 1 0 3 2 2 4 3 0 0 0 0 0 0 1 0 0 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3 10 6 15 16 24 9 36 1 0 1 7 8 1 0 0 0 0 0 0
Cytoplasm 68 281 132 140 151 250 213 282 200 213 170 262 271 308 358 426 279 210 85 276 126 141 256 225
Endoplasmic Reticulum 1 1 1 0 2 7 9 21 15 24 8 11 0 1 0 14 7 5 0 0 1 0 2 0
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 3 2 2 0 1 1 0 1 0
Golgi 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0 1 0 1
Mitochondria 4 6 6 6 2 14 18 22 35 48 49 90 1 1 0 9 0 0 0 4 1 0 1 6
Nucleus 0 1 0 0 0 0 2 3 1 2 3 2 0 0 0 5 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 1
Vac/Vac Membrane 0 0 1 0 5 4 22 36 11 24 16 22 3 2 1 10 11 2 0 2 1 0 0 4
Unique Cell Count 72 288 137 141 159 272 259 362 257 297 235 382 275 309 358 443 288 214 90 291 136 150 275 259
Labelled Cell Count 74 291 143 147 163 287 275 387 286 344 261 433 280 312 360 475 308 221 90 291 136 150 275 259


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.2 4.7 5.4 11.9 14.0 13.0 14.1 13.6 11.9 12.0 11.7 5.7 5.7 5.9 8.9 10.4 11.8 5.3 5.6 6.5
Std Deviation (1e-4) 1.0 1.0 1.0 1.3 4.2 5.2 4.7 5.0 4.6 4.2 4.6 4.2 1.2 1.3 1.6 3.1 2.7 5.1 1.2 2.1 1.9
Intensity Change (Log2) 0.19 1.34 1.57 1.47 1.59 1.54 1.34 1.36 1.32 0.28 0.28 0.34 0.92 1.15 1.33 0.18 0.25 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 3.4 0 3.7 0 0 0 0 0 0
Cytoplasm 1.7 -0.6 -1.7 -4.0 -4.9 -4.8 -5.9 -5.7 -6.5 1.4 2.8 3.6 -0.1 0.3 1.0
Endoplasmic Reticulum 0 0 0 0 2.5 2.4 3.1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 0 0.3 1.0 0.7 2.9 3.5 4.3 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 3.5 0 3.1 2.7 2.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.1312 0.0003 0.0238 0.0003 0.01 0.0034 0.0237 0.0011 0.0141 0.0071 0.0084 0.0096 0.0105 0.0003 0.0022 0.0004 0.0148 0.0002
Bud 0.0011 0.0001 0.0004 0.0001 0.0012 0.0028 0.0019 0.0033 0.0002 0.0014 0.0007 0.0005 0.0028 0.0042 0.0002 0.0001 0.0015 0.0001
Bud Neck 0.0008 0.0001 0.0006 0.0003 0.0012 0.0009 0.0007 0.0002 0.0003 0.0023 0.0004 0.0005 0.004 0.0003 0.0046 0.0002 0.0008 0.0002
Bud Periphery 0.001 0 0.0006 0.0001 0.0018 0.0071 0.0024 0.0045 0.0002 0.0017 0.0008 0.0008 0.0044 0.0006 0.0002 0.0001 0.0015 0
Bud Site 0.0054 0.0004 0.002 0.0001 0.0024 0.0015 0.0097 0.0094 0.0019 0.001 0.0002 0.0002 0.0259 0.0068 0.006 0.0001 0.0047 0
Cell Periphery 0.0003 0.0004 0.0006 0 0.0008 0.0001 0.0005 0.0001 0.0001 0.0003 0 0 0.0032 0.0005 0.0003 0 0.0003 0
Cytoplasm 0.0305 0.6899 0.5281 0.6181 0.5326 0.6325 0.3221 0.5326 0.481 0.4427 0.5522 0.4702 0.27 0.6523 0.4608 0.6546 0.6299 0.5138
Cytoplasmic Foci 0.183 0.0039 0.0159 0.0034 0.0224 0.0113 0.0291 0.0054 0.0149 0.0118 0.016 0.0098 0.0681 0.0445 0.0057 0.0032 0.0295 0.0024
Eisosomes 0.0007 0.0001 0.0005 0 0.0002 0.0001 0.0004 0 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0184 0.0039 0.012 0.0014 0.0063 0.0028 0.0029 0.0009 0.0024 0.0018 0.0019 0.001 0.0048 0.0021 0.0018 0.0011 0.0142 0.0006
Endosome 0.2332 0.0017 0.0183 0.0012 0.0078 0.0063 0.0172 0.001 0.0094 0.0035 0.0052 0.0042 0.0333 0.0006 0.0032 0.0008 0.0456 0.0004
Golgi 0.063 0.0001 0.0093 0.0001 0.0033 0.0008 0.0109 0.0007 0.006 0.0009 0.0009 0.0017 0.0064 0.0001 0.0007 0.0001 0.0126 0
Lipid Particles 0.1479 0.0001 0.0083 0.0001 0.0009 0.0007 0.0141 0.0001 0.0042 0.0003 0.0002 0.0013 0.033 0.0003 0.0026 0 0.0087 0
Mitochondria 0.0092 0.0001 0.0118 0.0001 0.0034 0.0006 0.0049 0.0003 0.002 0.0006 0.0005 0.0019 0.0031 0.0025 0.0003 0.0002 0.0034 0
None 0.031 0.2951 0.3536 0.3707 0.4013 0.3212 0.5104 0.4366 0.4331 0.51 0.4063 0.4916 0.4188 0.2777 0.4983 0.336 0.2045 0.4805
Nuclear Periphery 0.0148 0.001 0.0037 0.0005 0.0005 0.0012 0.003 0.0003 0.001 0.0021 0.0006 0.0009 0.0016 0.0004 0.0008 0.0005 0.0036 0.0002
Nucleolus 0.0009 0 0.0003 0 0.0001 0.0003 0.0035 0.0001 0.0002 0.0003 0.0001 0.0001 0.0147 0.0015 0.003 0 0.0003 0
Nucleus 0.002 0.0012 0.0017 0.0023 0.0007 0.0022 0.0089 0.0014 0.0057 0.0038 0.0017 0.0015 0.0091 0.0018 0.0034 0.0019 0.0018 0.0008
Peroxisomes 0.0875 0 0.0011 0.0001 0.002 0.0006 0.0249 0.0008 0.0204 0.0004 0.002 0.0016 0.0513 0.0002 0.0009 0.0001 0.0127 0
Punctate Nuclear 0.0336 0.0003 0.0011 0.0004 0.0005 0.0021 0.0062 0.0004 0.0016 0.0058 0.0012 0.0019 0.0092 0.002 0.0039 0.0003 0.007 0.0002
Vacuole 0.0026 0.001 0.0055 0.0006 0.0007 0.0015 0.0021 0.0006 0.0008 0.0015 0.0006 0.0005 0.0238 0.0012 0.0009 0.0004 0.0019 0.0002
Vacuole Periphery 0.0021 0.0001 0.0009 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002 0.0014 0.0002 0.0001 0.0001 0.0006 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.1462 16.2126 15.399 14.0452 9.876 14.5009 14.4255 13.4979 20.4444 16.2759
Translational Efficiency 1.0067 0.7554 0.6796 0.8645 1.2974 0.944 1.104 1.1756 0.7951 0.9245

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2002 1294 326 1480 1380 1602 176 26 3382 2896 502 1506

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 703.29 768.10 1007.14 976.38 746.22 758.88 1100.25 1047.83 720.81 763.00 1039.78 977.61
Standard Deviation 86.64 114.59 126.91 486.64 85.98 108.57 140.83 188.64 88.91 111.40 139.24 483.15
Intensity Change Log 2 0.127174 0.518073 0.473323 0.024271 0.560158 0.489732 0.075116 0.539892 0.481794

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000740 0.000615 0.000591 0.000080 0.000404 0.000609 0.000312 0.000054 0.000554 0.000613 0.000586
Bud Neck 0.008164 0.019805 0.002725 0.011630 0.005218 0.024496 0.001674 0.000735 0.006962 0.022400 0.002356 0.011442
Bud Site 0.002884 0.014716 0.009161 0.049583 0.004992 0.020864 0.006460 0.003115 0.003744 0.018117 0.008214 0.048780
Cell Periphery 0.000134 0.000115 0.000222 0.000314 0.000110 0.000130 0.000238 0.000296 0.000124 0.000123 0.000227 0.000314
Cytoplasm 0.625000* 0.481684* 0.362919* 0.566705* 0.652329* 0.511469* 0.455355* 0.476213* 0.636152* 0.498160* 0.395327* 0.565143*
Cytoplasmic Foci 0.123051 0.235872* 0.013499 0.016140 0.136214 0.215092* 0.016545 0.039490 0.128422 0.224377* 0.014567 0.016543
Eisosomes 0.000101 0.000102 0.000071 0.000027 0.000064 0.000113 0.000088 0.000135 0.000086 0.000108 0.000077 0.000029
Endoplasmic Reticulum 0.000490 0.001116 0.006658 0.001225 0.000794 0.000560 0.006653 0.002374 0.000614 0.000808 0.006656 0.001245
Endosome 0.005521 0.027001 0.004550 0.007371 0.006281 0.023947 0.007693 0.007350 0.005831 0.025312 0.005652 0.007371
Golgi 0.000699 0.004879 0.000136 0.004309 0.000668 0.005305 0.000191 0.000164 0.000686 0.005114 0.000155 0.004237
Lipid Particles 0.003547 0.005385 0.002660 0.000681 0.002974 0.004701 0.003241 0.003010 0.003313 0.005007 0.002864 0.000721
Mitochondria 0.001277 0.005643 0.000398 0.002213 0.001293 0.007536 0.000241 0.000161 0.001284 0.006690 0.000343 0.002178
Mitotic Spindle 0.001773 0.002207 0.001728 0.057780 0.001624 0.013136 0.000786 0.000167 0.001712 0.008252 0.001398 0.056785
None 0.006718 0.003053 0.003396 0.007493 0.004893 0.002154 0.002689 0.005162 0.005973 0.002556 0.003148 0.007452
Nuclear Periphery 0.000273 0.000552 0.001860 0.001304 0.000276 0.000261 0.001355 0.000772 0.000274 0.000391 0.001683 0.001295
Nuclear Periphery Foci 0.000316 0.001003 0.004769 0.002816 0.000899 0.000595 0.010501 0.003097 0.000554 0.000777 0.006779 0.002821
Nucleolus 0.000678 0.001349 0.000576 0.000301 0.000952 0.001207 0.000322 0.001024 0.000790 0.001271 0.000487 0.000313
Nucleus 0.177189* 0.082562 0.451276* 0.149607* 0.137331* 0.070667 0.238185* 0.165012* 0.160925* 0.075982 0.376567* 0.149873*
Peroxisomes 0.001054 0.012364 0.000306 0.001770 0.001099 0.010902 0.000376 0.000429 0.001073 0.011556 0.000331 0.001747
Vacuole 0.040627 0.097059 0.132257 0.112809 0.041119 0.084068 0.246504* 0.290619* 0.040828 0.089873 0.172312 0.115879
Vacuole Periphery 0.000468 0.002793 0.000219 0.005331 0.000790 0.002395 0.000293 0.000364 0.000600 0.002573 0.000245 0.005245

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.99 -11.34 -8.52 1.15 3.95 -6.98 -8.45 -2.40 2.06 4.41 -4.62 -13.92 -8.14 0.82 4.10
Bud Neck -7.05 6.06 1.93 8.25 -3.81 -10.79 4.50 5.05 14.19 1.49 -12.51 7.28 0.89 12.45 -4.85
Bud Site -9.57 -2.31 -8.93 -3.04 -3.62 -7.91 -0.34 -0.23 8.05 0.12 -12.14 -2.25 -8.47 -1.55 -4.97
Cell Periphery 2.82 -6.35 -2.84 -3.73 1.75 -1.50 -4.82 -0.71 -0.37 1.15 0.41 -8.06 -3.31 -3.36 1.50
Cytoplasm 15.42 22.00 17.73 0.52 -10.72 14.57 14.47 6.13 3.17 -0.01 20.80 26.32 20.75 2.79 -11.05
Cytoplasmic Foci -16.65 32.98 33.83 37.47 0.21 -11.62 27.83 10.30 17.24 -2.03 -20.30 43.08 43.27 52.65 0.22
Eisosomes -0.02 1.81 14.84 14.10 9.15 -4.36 -3.12 -1.46 0.99 -0.13 -3.10 -0.39 16.59 11.41 11.35
Endoplasmic Reticulum -5.32 -14.53 -8.37 -2.61 12.61 2.15 -7.97 -2.10 -2.63 5.54 -2.61 -16.36 -7.93 -6.26 14.14
Endosome -11.99 -0.57 4.92 13.71 5.41 -11.03 0.02 1.72 10.76 1.53 -16.36 -0.34 6.27 18.77 6.13
Golgi -6.52 2.87 -0.90 5.39 -2.53 -7.24 4.95 5.11 8.18 0.96 -9.63 4.45 -0.96 7.24 -2.54
Lipid Particles -3.19 0.86 7.16 9.16 7.18 -2.23 -0.06 2.13 3.56 2.26 -3.43 0.65 9.01 9.23 9.32
Mitochondria -4.64 1.91 0.51 5.56 -4.53 -6.27 2.61 2.96 8.26 3.70 -7.74 2.96 0.77 8.94 -5.05
Mitotic Spindle -0.44 -0.56 -9.78 -9.82 -9.58 -5.33 1.36 -0.62 3.33 -1.05 -5.11 -0.11 -9.96 -7.32 -9.95
None 3.69 3.85 -0.09 -5.67 -5.98 3.80 4.49 1.78 -0.02 -0.43 5.33 5.30 -1.00 -7.65 -7.50
Nuclear Periphery -1.91 -17.60 -16.82 -6.88 4.39 0.20 -12.72 -3.81 -3.85 4.15 -1.78 -21.28 -17.07 -12.30 3.63
Nuclear Periphery Foci -3.52 -8.66 -4.85 -1.85 6.46 1.48 -7.28 -3.34 -4.14 5.09 -1.43 -11.18 -3.80 -3.01 8.86
Nucleolus -4.55 0.50 5.77 7.47 2.10 -0.74 2.72 1.53 2.56 -0.75 -2.84 2.22 4.12 7.50 1.73
Nucleus 19.14 -15.22 -3.51 -19.31 13.56 14.53 -4.78 2.25 -1.15 4.95 25.14 -14.85 -6.47 -22.65 11.42
Peroxisomes -8.64 5.78 1.80 8.89 -0.70 -10.85 6.14 5.69 11.93 -0.32 -13.66 8.28 2.05 13.71 -0.82
Vacuole -15.38 -13.13 -28.30 -17.16 -1.95 -13.04 -16.02 -8.45 -7.59 -2.12 -20.25 -19.48 -29.17 -20.45 1.90
Vacuole Periphery -4.98 2.17 -1.81 2.30 -2.35 -3.96 2.48 2.53 6.53 0.44 -6.66 3.25 -1.50 2.45 -2.36