Standard name
Human Ortholog
Description Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.06 0.07 0.18 0.27 0.37 0.45 0.41 0.52 0.53 0.59 0.59 0.13 0.31 0.44 0.18 0.21 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.39 0.27 0.45 0.33 0.22 0.15 0.16 0.08 0.08 0.06 0 0.05 0.66 0.54 0.43 0.27 0.25 0.22 0.43 0.42 0.54 0.53 0.41 0.28
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.16 0.14 0.12 0.16 0.2 0.23 0.2 0.32 0.25 0.29 0 0 0 0.06 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.84 0.59 0.54 0.6 0.44 0.4 0.34 0.31 0.29 0.24 0.23 0.48 0.39 0.3 0.69 0.7 0.69 0.49 0.51 0.38 0.35 0.49 0.62
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 1 0 1 1 1 0 0 0 0 1 0 0 0 1 0 0 0
Bud 0 1 10 3 5 1 8 13 10 20 7 2 0 8 3 1 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 56 33 33 48 117 159 256 230 212 252 113 115 61 167 214 86 56 44 0 5 1 3 4 5
Endoplasmic Reticulum 0 3 0 2 6 12 10 13 9 9 2 5 0 3 2 12 9 12 3 1 1 2 2 5
Endosome 196 138 205 87 97 63 91 45 33 28 8 9 309 285 210 130 66 54 219 228 256 141 141 110
Golgi 0 3 0 0 1 1 0 0 0 0 0 0 0 0 0 1 4 1 8 12 6 3 1 4
Mitochondria 62 20 74 38 53 71 112 126 80 150 48 57 5 3 4 27 6 11 8 13 13 12 12 13
Nucleus 5 6 6 1 5 2 2 12 0 3 0 2 3 0 2 17 13 7 2 2 1 0 2 1
Nuclear Periphery 0 2 0 3 4 2 1 4 0 0 1 0 0 0 0 1 0 0 7 2 2 2 5 4
Nucleolus 35 21 17 19 8 5 3 13 1 3 0 0 4 2 1 1 0 2 2 0 1 0 0 0
Peroxisomes 4 0 2 0 0 1 0 0 0 0 0 0 5 12 8 1 1 0 0 0 0 2 0 0
SpindlePole 12 5 36 10 5 5 5 12 2 3 2 2 36 20 20 9 4 1 2 3 5 1 1 0
Vac/Vac Membrane 341 435 265 144 262 192 230 188 125 137 45 44 227 208 146 329 187 173 249 278 181 93 171 246
Unique Cell Count 509 518 451 265 435 432 574 558 404 474 190 195 470 532 490 478 267 249 512 550 478 264 350 398
Labelled Cell Count 711 667 648 355 563 515 722 658 472 607 227 237 650 708 610 615 347 305 512 550 478 264 350 398


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.1 5.5 5.4 5.4 5.1 4.8 4.7 5.1 4.5 4.7 4.7 5.8 5.4 5.3 7.0 8.4 7.8 5.4 5.6 6.1
Std Deviation (1e-4) 1.2 1.2 1.0 1.1 1.3 1.1 1.1 1.0 1.2 1.1 0.8 1.0 1.2 1.3 1.4 1.6 1.7 1.9 1.1 1.1 1.1
Intensity Change (Log2) -0.03 -0.03 -0.11 -0.21 -0.24 -0.13 -0.31 -0.22 -0.24 0.08 -0.03 -0.07 0.35 0.6 0.49 -0.03 0.01 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.2 -2.7 0 0 0.2 0 0 0 0 -0.8 -2.1 -2.8 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.4 7.8 10.6 13.2 12.2 14.6 15.1 14.4 14.3 2.8 9.3 12.7 4.9 5.4 4.2
Endoplasmic Reticulum 0 0 3.6 0 0 0 0 0 0 0 0 0 3.4 0 0
Endosome -3.3 -7.3 -10.0 0 0 -12.1 0 0 0 6.2 2.5 -0.8 -5.8 -5.5 -6.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.7 -1.8 0 0 0 1.3 0 0 0 -8.3 -9.2 -8.7 -5.3 -5.8 -4.6
Nucleus 0 -0.2 0 0 1.0 0 0 0 0 0 0 0 2.2 2.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 -1.7 -2.5 0 0 -3.6 0 0 0 -3.0 -3.9 -4.0 -3.9 -3.2 -2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 2.4 0 0 0 0
SpindlePole -2.2 -4.8 -4.8 0 0 -5.3 0 0 0 -0.2 -2.8 -2.5 -4.3 -3.7 -4.3
Vacuole -1.2 0.4 -4.3 -5.9 -8.0 -8.2 -9.2 -8.1 -8.5 -3.2 -6.1 -8.9 3.2 3.0 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7539 4.6207 4.8543 4.0858 4.0273 4.4262 3.0193 4.271 4.1772 2.609 4.1041 4.2291 4.9827 5.6709 5.435 5.2715 6.0498 5.4243
Actin 0.002 0.0002 0.0166 0.0001 0.0001 0.002 0.0142 0.0038 0.0096 0 0.0001 0.0001 0.0141 0.0001 0.0021 0.0001 0.0072 0.004
Bud 0.0003 0.0001 0.0004 0.0003 0.0001 0.0011 0.0003 0.0008 0.0034 0 0.0013 0.0004 0.0003 0.0006 0.0004 0.0003 0.0006 0.0003
Bud Neck 0.0004 0.0007 0.0016 0.0007 0.0002 0.0017 0.0004 0.0007 0.0007 0.0001 0.0013 0.001 0.0006 0.0007 0.0008 0.0004 0.0025 0.001
Bud Periphery 0.0007 0.0002 0.0008 0.0002 0.0001 0.0024 0.0003 0.0005 0.0036 0 0.001 0.0005 0.0006 0.0006 0.0004 0.0008 0.0008 0.0006
Bud Site 0.0005 0.0007 0.0019 0.0057 0.0002 0.0013 0.0008 0.0111 0.0049 0.0001 0.0043 0.0003 0.0011 0.0087 0.0028 0.0007 0.0035 0.0007
Cell Periphery 0.0008 0.0005 0.0005 0.0001 0.0001 0.0009 0.0003 0.0005 0.0004 0.0001 0.0007 0.0005 0.0003 0.0005 0.0002 0.0004 0.0004 0.0004
Cytoplasm 0.0207 0.0213 0.0097 0.0124 0.0026 0.0077 0.01 0.0289 0.0083 0.0017 0.0094 0.0097 0.0136 0.0246 0.026 0.0036 0.0157 0.0139
Cytoplasmic Foci 0.1334 0.0517 0.0512 0.0491 0.0441 0.0458 0.0439 0.1462 0.065 0.0183 0.1015 0.0725 0.0528 0.0594 0.0832 0.0199 0.0578 0.0334
Eisosomes 0.0022 0 0.0011 0 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.031 0.0077 0.0041 0.0022 0.0012 0.0029 0.0041 0.0033 0.0051 0.0007 0.0017 0.0019 0.0051 0.0032 0.0039 0.0024 0.0056 0.0038
Endosome 0.4437 0.5876 0.4671 0.7868 0.6287 0.5471 0.6375 0.6093 0.5304 0.6849 0.5316 0.5888 0.614 0.4941 0.6006 0.6237 0.429 0.5263
Golgi 0.026 0.0201 0.0409 0.0488 0.0572 0.0375 0.0342 0.0348 0.0385 0.0085 0.0102 0.03 0.026 0.017 0.0332 0.017 0.0236 0.0199
Lipid Particles 0.0309 0.0778 0.096 0.0092 0.0194 0.0647 0.0373 0.0357 0.0258 0.048 0.0044 0.0713 0.0389 0.0388 0.0222 0.0078 0.0645 0.0606
Mitochondria 0.0351 0.0046 0.0347 0.0047 0.0013 0.009 0.0179 0.0044 0.0613 0.0004 0.0027 0.0131 0.0105 0.0081 0.0063 0.0105 0.0077 0.0096
None 0.1145 0.0291 0.0565 0.0006 0.0001 0.0005 0.0023 0.0064 0.0258 0.0001 0.0209 0.0037 0.0041 0.0106 0.0164 0.0004 0.0197 0.0163
Nuclear Periphery 0.0127 0.0046 0.0047 0.002 0.0017 0.0075 0.0048 0.0013 0.0097 0.0014 0.0021 0.0024 0.0091 0.0062 0.0035 0.0066 0.0052 0.0114
Nucleolus 0.0009 0.0017 0.0052 0.0002 0.0002 0.002 0.0005 0.0041 0.0014 0.0002 0.0043 0.0015 0.0016 0.0018 0.004 0.0009 0.0009 0.0028
Nucleus 0.0014 0.0017 0.003 0.0006 0.0004 0.0029 0.0024 0.0015 0.0012 0.0003 0.0047 0.0011 0.0071 0.0023 0.0019 0.0023 0.0032 0.0041
Peroxisomes 0.0018 0.003 0.0058 0.0018 0.0011 0.0092 0.0128 0.0116 0.0156 0.0014 0.0015 0.0052 0.0052 0.003 0.0067 0.0006 0.0217 0.0017
Punctate Nuclear 0.0018 0.0011 0.0085 0.0003 0.0002 0.0007 0.0019 0.0016 0.0006 0.0002 0.0048 0.0006 0.0011 0.0069 0.0021 0.0003 0.0021 0.0015
Vacuole 0.117 0.142 0.1474 0.0539 0.1804 0.1865 0.1497 0.0793 0.135 0.1964 0.2538 0.1458 0.1526 0.2451 0.1379 0.246 0.2373 0.2215
Vacuole Periphery 0.0222 0.0435 0.042 0.0202 0.0608 0.0665 0.0244 0.0142 0.0534 0.0372 0.0374 0.0494 0.0412 0.0678 0.0454 0.0555 0.091 0.0658

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3592 14.918 15.4977 23.8982 25.0985 19.3473 17.1952 23.8221 17.6427 24.8419
Translational Efficiency 0.4148 0.523 0.4509 0.3276 0.3337 0.5541 0.4739 0.2922 0.5066 0.2682

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1748 1321 1835 1670 1670 1987 351 812 3418 3308 2186 2482

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 801.80 851.35 1312.21 1276.14 870.67 961.51 1332.72 1985.72 835.45 917.52 1315.50 1508.28
Standard Deviation 118.04 164.52 190.59 248.31 132.07 200.60 182.92 398.17 129.74 194.65 189.53 451.87
Intensity Change Log 2 0.086510 0.710684 0.670472 0.143176 0.614176 1.189464 0.116287 0.661249 0.963715

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000152 0.000134 0.000872 0.000173 0.000360 0.000365 0.000200 0.000126 0.000277 0.000171 0.000652
Bud Neck 0.001481 0.001819 0.000851 0.003580 0.001214 0.002387 0.001499 0.004982 0.001351 0.002160 0.000955 0.004039
Bud Site 0.003907 0.007252 0.002823 0.015274 0.005124 0.008675 0.005014 0.012784 0.004501 0.008107 0.003175 0.014460
Cell Periphery 0.000069 0.000089 0.000052 0.000070 0.000086 0.000048 0.000121 0.000032 0.000077 0.000064 0.000063 0.000058
Cytoplasm 0.808488* 0.819900* 0.706456* 0.694678* 0.791495* 0.790027* 0.569087* 0.887112* 0.800185* 0.801956* 0.684399* 0.757634*
Cytoplasmic Foci 0.071663 0.048948 0.003984 0.015690 0.072890 0.067656 0.014568 0.005779 0.072262 0.060185 0.005683 0.012448
Eisosomes 0.000017 0.000012 0.000004 0.000010 0.000020 0.000015 0.000029 0.000003 0.000019 0.000014 0.000008 0.000008
Endoplasmic Reticulum 0.000477 0.000836 0.000512 0.000648 0.000948 0.000964 0.002196 0.000024 0.000707 0.000913 0.000782 0.000444
Endosome 0.002776 0.004856 0.000436 0.009182 0.003577 0.006788 0.003807 0.000324 0.003167 0.006016 0.000977 0.006284
Golgi 0.001306 0.001441 0.000086 0.003987 0.001471 0.002025 0.000222 0.000397 0.001387 0.001792 0.000108 0.002813
Lipid Particles 0.000905 0.000734 0.000308 0.002836 0.000988 0.000992 0.001273 0.000080 0.000946 0.000889 0.000463 0.001935
Mitochondria 0.000713 0.000764 0.000203 0.003360 0.000796 0.001225 0.000129 0.002783 0.000754 0.001041 0.000191 0.003171
Mitotic Spindle 0.002132 0.001796 0.003918 0.079928 0.001476 0.009554 0.004203 0.007466 0.001811 0.006456 0.003964 0.056221
None 0.011367 0.011981 0.009875 0.007578 0.006001 0.004411 0.004456 0.007505 0.008746 0.007434 0.009005 0.007554
Nuclear Periphery 0.000097 0.000156 0.000386 0.000455 0.000133 0.000208 0.000943 0.000050 0.000115 0.000187 0.000475 0.000323
Nuclear Periphery Foci 0.001396 0.002010 0.001312 0.001801 0.002098 0.000901 0.006339 0.000030 0.001739 0.001344 0.002119 0.001221
Nucleolus 0.000551 0.000767 0.000101 0.000227 0.000413 0.000455 0.000176 0.000280 0.000483 0.000580 0.000113 0.000244
Nucleus 0.053975 0.045507 0.246378* 0.064715 0.067852 0.038201 0.135979* 0.046491 0.060755 0.041118 0.228651* 0.058753
Peroxisomes 0.000411 0.001870 0.000049 0.000893 0.000542 0.001658 0.000152 0.003169 0.000475 0.001743 0.000066 0.001638
Vacuole 0.037616 0.048051 0.022071 0.090268 0.042014 0.062264 0.249156* 0.019477 0.039765 0.056588 0.058534 0.067108
Vacuole Periphery 0.000570 0.001058 0.000062 0.003948 0.000688 0.001186 0.000287 0.001031 0.000628 0.001135 0.000098 0.002994

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.64 -7.49 -2.65 -1.83 -1.16 -1.51 -6.00 1.40 2.01 5.99 -2.11 -6.38 -1.95 0.19 -0.08
Bud Neck -1.23 -0.60 -3.45 -1.63 -3.30 -4.95 -4.48 -3.66 -1.83 -2.06 -4.33 -2.26 -5.28 -2.06 -3.94
Bud Site -4.27 0.56 -3.91 -0.31 -3.93 -2.90 -2.03 -1.54 0.59 -0.18 -4.86 0.69 -3.72 0.37 -3.94
Cell Periphery -1.31 -1.91 3.87 2.80 6.15 4.24 -4.43 6.94 7.39 12.76 2.07 -2.10 6.97 3.86 11.61
Cytoplasm -0.82 24.46 23.72 22.33 2.39 0.80 20.48 -10.28 -10.71 -25.27 0.64 30.31 15.94 14.63 -11.54
Cytoplasmic Foci 6.10 25.83 23.83 16.36 -6.39 1.65 22.15 25.17 23.78 6.95 4.71 35.72 34.29 28.28 -2.93
Eisosomes 2.99 7.63 6.24 2.97 -2.60 4.23 -4.60 15.80 13.41 14.69 4.85 7.77 13.39 9.07 6.34
Endoplasmic Reticulum -2.05 -2.83 2.56 3.11 5.12 0.14 -3.56 10.84 2.65 8.49 -0.71 -2.06 9.03 3.02 9.25
Endosome -3.13 9.70 1.01 3.35 -3.90 -4.69 2.44 13.45 10.85 10.89 -5.87 12.49 4.78 8.32 -2.14
Golgi -0.08 3.06 1.01 1.05 -2.46 -0.64 2.03 2.00 4.51 -0.13 -0.68 3.50 1.85 3.13 -2.55
Lipid Particles 1.22 1.80 -1.07 -1.72 -1.96 0.19 -4.15 9.82 4.27 16.38 0.62 1.76 0.25 -0.14 -0.49
Mitochondria 0.01 1.54 -0.21 -0.17 -2.00 -0.87 2.09 -1.04 -0.56 -2.08 -0.68 2.41 -0.83 -0.19 -2.82
Mitotic Spindle 0.68 -2.36 -8.92 -9.27 -7.96 -4.59 -3.46 -1.79 2.62 1.44 -3.82 -3.63 -9.16 -6.93 -7.33
None 0.06 2.60 6.24 4.05 9.96 1.85 1.22 0.06 -2.19 -1.37 1.70 1.11 5.26 2.76 8.03
Nuclear Periphery -1.68 -22.22 -19.39 -12.04 1.34 -1.60 -17.84 2.85 2.35 18.49 -2.31 -26.61 -17.58 -8.29 9.73
Nuclear Periphery Foci -2.14 -2.45 0.53 3.01 3.61 6.24 -8.96 13.58 9.20 14.53 2.36 -4.69 5.96 3.83 10.35
Nucleolus -0.81 2.19 1.61 5.13 -7.76 -0.74 2.82 1.29 1.61 -2.33 -0.88 3.22 2.08 3.74 -6.62
Nucleus 2.79 -44.67 -13.66 -14.58 34.12 11.00 -10.11 1.63 -7.74 10.69 10.29 -43.47 -9.60 -18.77 36.76
Peroxisomes -1.88 6.12 1.51 2.06 -3.63 -2.64 4.22 -1.70 -0.17 -2.32 -3.19 7.32 -1.18 2.08 -3.01
Vacuole -4.53 -7.52 -28.94 -25.14 -22.50 -7.65 -18.69 0.21 6.84 18.59 -9.26 -15.16 -26.01 -20.33 -9.16
Vacuole Periphery -1.42 3.30 -0.79 0.45 -2.70 -1.40 3.13 0.32 1.33 -1.22 -2.02 4.84 -0.46 1.16 -3.03