Standard name
Description FAD pyrophosphatase; hydrolyzes FAD as the preferred substrate but also acts on NADH and ADP-ribose at lower rates; activity is dependent upon K+ and divalent metal cations; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; FPY1 is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.99 0.99 0.96 0.92 0.95 0.79 0.71 0.64 0.67 0.67 0.82 0.96 0.97 0.98 0.96 0.92 0.95 0.92 0.93 0.96 0.9 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.21 0.05 0.14 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.16 0.24 0.2 0.27 0.15 0.08 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 3 0 0 1 0 0 0 0 0 0 1 0 1 1 1
Bud 0 0 0 1 0 0 0 1 5 2 2 0 0 1 0 0 0 0 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 2 2 4 2 4 4 6 9 1 1 1 2 11 10 6 0 0 0 0 0 0
Cytoplasm 228 450 110 138 221 141 143 184 140 145 146 107 246 211 307 646 569 472 247 430 121 203 238 258
Endoplasmic Reticulum 2 2 0 1 4 1 2 3 1 1 5 1 1 2 2 29 20 14 0 2 1 3 4 4
Endosome 0 0 0 0 0 0 0 1 1 0 0 0 0 1 3 1 1 2 2 2 0 2 5 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 4 5 4 3 0 0 3 1
Mitochondria 2 1 0 2 3 1 5 13 46 10 30 13 0 0 0 5 6 3 0 4 0 0 3 0
Nucleus 2 3 1 1 8 5 4 6 6 4 8 4 1 4 1 6 17 13 1 4 1 4 2 2
Nuclear Periphery 0 3 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 1 0 1 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 9 5 0 4 14 4 28 63 43 58 33 11 9 9 8 33 67 37 8 7 0 4 1 2
Unique Cell Count 242 456 111 144 241 149 180 259 220 216 219 131 256 217 314 672 621 498 269 464 127 225 265 284
Labelled Cell Count 245 465 113 149 253 156 185 275 248 229 233 138 261 230 323 731 696 552 269 464 127 225 265 284


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 9.1 8.8 8.7 8.4 9.2 9.7 10.1 8.4 9.7 9.1 9.2 10.0 9.7 9.4 8.5 10.4 10.4 11.2 11.4 11.4
Std Deviation (1e-4) 1.7 1.4 1.4 1.6 1.8 1.6 1.6 1.8 2.0 2.0 1.9 2.0 2.0 1.3 1.5 1.2 1.5 1.6 2.0 2.3 2.0
Intensity Change (Log2) – – – -0.01 -0.07 0.07 0.14 0.2 -0.07 0.14 0.04 0.07 0.18 0.13 0.09 -0.05 0.24 0.24 0.34 0.37 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.7 -1.9 -4.8 -6.1 -7.1 -6.6 -6.7 -4.4 -1.6 -1.1 -0.9 -1.6 -2.8 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.2 0 4.1 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.4 5.7 5.0 6.0 4.3 3.1 0 0 0 0 3.6 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9948 8.3817 7.3385 5.6288 6.4836 7.797 15.5384 16.3932 15.866 15.1389 14.5145 16.1465 11.963 13.4492 12.643 11.1006 12.0824 12.7844
Actin 0.0566 0.0007 0.0407 0.0054 0.0443 0.0019 0.0039 0.0002 0.0014 0.0009 0.0001 0.001 0.0035 0.005 0.0057 0.0349 0.0005 0.0007
Bud 0.0018 0.0001 0.0004 0.0032 0.0063 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0001
Bud Neck 0.0052 0.0002 0.0013 0.0013 0.0007 0.0008 0.0004 0.0003 0.0005 0.0005 0.0005 0.001 0.0006 0.0009 0.0007 0.0005 0.0004 0.0009
Bud Periphery 0.0028 0 0.0004 0.0027 0.0093 0.0001 0.0001 0 0 0 0 0 0.0002 0 0 0.0002 0 0
Bud Site 0.0146 0.0002 0.0017 0.0422 0.0076 0.0001 0.0003 0.0003 0.0003 0.0001 0 0.0001 0.0005 0.0003 0.0002 0.0004 0 0.0001
Cell Periphery 0.0007 0 0.0001 0.0003 0.0003 0 0 0 0 0 0 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.6438 0.9799 0.8831 0.6892 0.7719 0.8985 0.9456 0.958 0.9591 0.9586 0.9577 0.937 0.9339 0.9741 0.9589 0.9164 0.9403 0.957
Cytoplasmic Foci 0.0346 0.0015 0.0065 0.0666 0.0197 0.0132 0.0021 0.0019 0.002 0.0038 0.001 0.0039 0.0046 0.0014 0.0039 0.0022 0.0015 0.0019
Eisosomes 0.0007 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0 0 0 0 0.0004 0 0
Endoplasmic Reticulum 0.0076 0.0013 0.0023 0.0009 0.0049 0.0029 0.0022 0.0014 0.0022 0.0031 0.0011 0.0024 0.0049 0.0011 0.0015 0.0015 0.0029 0.0029
Endosome 0.0352 0.0005 0.012 0.0627 0.0438 0.0123 0.0018 0.001 0.0016 0.0019 0.0005 0.0029 0.0058 0.0004 0.0013 0.0027 0.0086 0.0011
Golgi 0.0123 0.0001 0.0118 0.0354 0.0122 0.0044 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0007 0.0001 0.0004 0.0034 0.0004 0.0001
Lipid Particles 0.0154 0 0.008 0.0079 0.0074 0.0111 0.0001 0 0 0 0 0.0004 0.0006 0 0.0003 0.0014 0.0007 0.0001
Mitochondria 0.0262 0.0001 0.0099 0.0221 0.0041 0.0009 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0.001 0.0001 0.0002 0.0179 0.0003 0.0002
None 0.0133 0.0016 0.0012 0.0009 0.0037 0.0012 0.0059 0.0034 0.0036 0.0013 0.0013 0.0034 0.0071 0.002 0.0026 0.002 0.0018 0.0035
Nuclear Periphery 0.0331 0.0019 0.0026 0.0009 0.0256 0.012 0.0035 0.0024 0.0041 0.0022 0.0021 0.0032 0.0056 0.001 0.002 0.0019 0.0187 0.002
Nucleolus 0.0018 0 0.0002 0.0011 0.0008 0.0001 0.0002 0 0 0 0 0 0.0002 0 0 0 0.0001 0
Nucleus 0.0685 0.0105 0.011 0.0031 0.0268 0.0325 0.0311 0.0287 0.0232 0.0263 0.034 0.0413 0.0262 0.0125 0.0198 0.0116 0.0209 0.0282
Peroxisomes 0.0057 0 0.0043 0.0466 0.0013 0.0023 0.0002 0.0001 0.0001 0.0001 0 0.0003 0.0007 0.0002 0.0007 0.0011 0.0001 0.0001
Punctate Nuclear 0.0132 0.0009 0.0005 0.0036 0.0054 0.0035 0.0014 0.001 0.0009 0.0005 0.0006 0.0017 0.0021 0.0003 0.0015 0.0004 0.0015 0.0008
Vacuole 0.0045 0.0005 0.0011 0.0029 0.0023 0.0013 0.0006 0.0007 0.0003 0.0003 0.0007 0.0005 0.0013 0.0003 0.0002 0.0006 0.0006 0.0004
Vacuole Periphery 0.0023 0.0001 0.0006 0.0009 0.0017 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0003 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.1362 39.455 39.9237 47.8233 46.82 41.4383 42.6129 35.9001 43.6546 45.579
Translational Efficiency 1.2487 1.5983 1.4952 1.4788 1.3144 1.3476 1.5171 1.928 1.5043 1.5146

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘