Standard name
Description FAD pyrophosphatase; hydrolyzes FAD as the preferred substrate but also acts on NADH and ADP-ribose at lower rates; activity is dependent upon K+ and divalent metal cations; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; FPY1 is not an essential gene; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.99 0.99 0.96 0.92 0.95 0.79 0.71 0.64 0.67 0.67 0.82 0.96 0.97 0.98 0.96 0.92 0.95 0.92 0.93 0.96 0.9 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.21 0.05 0.14 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.16 0.24 0.2 0.27 0.15 0.08 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 3 0 0 1 0 0 0 0 0 0 1 0 1 1 1
Bud 0 0 0 1 0 0 0 1 5 2 2 0 0 1 0 0 0 0 0 1 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 2 2 2 4 2 4 4 6 9 1 1 1 2 11 10 6 0 0 0 0 0 0
Cytoplasm 228 450 110 138 221 141 143 184 140 145 146 107 246 211 307 646 569 472 247 430 121 203 238 258
Endoplasmic Reticulum 2 2 0 1 4 1 2 3 1 1 5 1 1 2 2 29 20 14 0 2 1 3 4 4
Endosome 0 0 0 0 0 0 0 1 1 0 0 0 0 1 3 1 1 2 2 2 0 2 5 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 4 5 4 3 0 0 3 1
Mitochondria 2 1 0 2 3 1 5 13 46 10 30 13 0 0 0 5 6 3 0 4 0 0 3 0
Nucleus 2 3 1 1 8 5 4 6 6 4 8 4 1 4 1 6 17 13 1 4 1 4 2 2
Nuclear Periphery 0 3 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 1 0 1 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 9 5 0 4 14 4 28 63 43 58 33 11 9 9 8 33 67 37 8 7 0 4 1 2
Unique Cell Count 242 456 111 144 241 149 180 259 220 216 219 131 256 217 314 672 621 498 269 464 127 225 265 284
Labelled Cell Count 245 465 113 149 253 156 185 275 248 229 233 138 261 230 323 731 696 552 269 464 127 225 265 284


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 9.1 8.8 8.7 8.4 9.2 9.7 10.1 8.4 9.7 9.1 9.2 10.0 9.7 9.4 8.5 10.4 10.4 11.2 11.4 11.4
Std Deviation (1e-4) 1.7 1.4 1.4 1.6 1.8 1.6 1.6 1.8 2.0 2.0 1.9 2.0 2.0 1.3 1.5 1.2 1.5 1.6 2.0 2.3 2.0
Intensity Change (Log2) -0.01 -0.07 0.07 0.14 0.2 -0.07 0.14 0.04 0.07 0.18 0.13 0.09 -0.05 0.24 0.24 0.34 0.37 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.7 -1.9 -4.8 -6.1 -7.1 -6.6 -6.7 -4.4 -1.6 -1.1 -0.9 -1.6 -2.8 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.2 0 4.1 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.4 5.7 5.0 6.0 4.3 3.1 0 0 0 0 3.6 3.0