Standard name
Description Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.13 0.06 0.17 0.23 0.22 – 0.21 0.18 0.21 0.16 0.1 0.14 0.23 0.12 0.09 0.08 0 0 0.06 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.08 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.2 0 0.1 0.05 0.48 0.17 – 0.45 0.49 0.5 0.5 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.91 0.74 0.79 0.82 0.68 0.44 0.5 – 0.41 0.44 0.43 0.46 0.85 0.79 0.63 0.79 0.76 0.76 0.85 0.72 0.72 0.75 0.59 0.44
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.18 0.05 0 0.1 0.06 0.12 – 0.06 0.06 0.05 0.06 0.06 0.08 0.16 0.11 0.15 0.19 0.08 0.15 0.15 0.14 0.21 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 1 2 0 – 12 8 11 8 0 1 0 0 0 0 6 3 2 2 10 11
Bud Neck 0 0 2 0 1 2 1 – 5 1 2 3 0 0 0 0 0 0 0 0 0 0 0 4
Bud Site 0 0 0 0 0 6 1 – 4 6 5 9 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 11 30 20 62 73 106 – 89 85 71 86 20 52 82 16 7 12 6 2 16 4 11 15
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 0 0 1 1 1 8 2 3 0 0 0 0 0 3
Endosome 0 0 4 8 20 3 36 – 14 11 4 3 0 3 5 0 1 2 0 2 0 1 2 14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 0 0 2 1 1
Mitochondria 7 27 7 32 16 154 82 – 187 227 168 277 3 8 1 3 4 7 2 3 1 2 5 7
Nucleus 345 101 179 270 241 142 238 – 173 207 144 256 171 291 223 105 62 121 345 111 197 139 192 143
Nuclear Periphery 0 0 0 0 1 0 0 – 2 2 1 3 0 0 0 0 1 1 0 0 0 0 1 2
Nucleolus 0 0 0 2 0 0 1 – 1 1 4 5 0 0 1 0 0 0 0 0 0 2 13 12
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 6 6 14 – 6 11 4 9 0 2 0 0 0 0 6 3 9 2 12 14
Vac/Vac Membrane 24 24 12 13 34 19 55 – 24 29 18 31 12 28 58 15 12 30 32 23 41 25 67 86
Unique Cell Count 378 136 226 329 355 321 477 419 467 338 552 202 370 353 133 82 160 406 154 275 186 327 323
Labelled Cell Count 392 166 235 348 382 407 534 517 588 432 690 207 388 372 147 89 177 406 154 275 186 327 323


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.5 5.6 5.5 6.0 4.3 5.5 – 4.3 4.5 4.4 4.4 6.0 5.2 6.2 6.6 7.4 7.5 5.2 5.4 5.4
Std Deviation (1e-4) 0.6 0.6 1.5 1.5 1.9 1.9 4.4 – 1.5 1.6 1.5 3.3 1.3 1.2 1.7 1.2 1.8 1.5 1.6 1.5 1.2
Intensity Change (Log2) – – – -0.01 0.11 -0.39 -0.01 – -0.39 -0.31 -0.34 -0.33 0.12 -0.09 0.14 0.24 0.42 0.42 -0.1 -0.05 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.1 2.7 4.2