Standard name
Description Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ATG41, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.31 0.39 0.78 0.61 0.58 – 0.66 0.71 0.69 0.69 0.62 0.37 0.47 0.64 0.54 0.56 0.52 0 0 0.12 0 0 0
Endoplasmic Reticulum 0 0 0.06 0.07 0 0.07 – 0 0 0 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 – 0 0 0 0 0 0.09 0.2 0.14 0.16 0.15 0.14 0.17 0.2 0.13 0.2 0.27 0.32
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.44 0.15 0.14 0.42 – 0.42 0.47 0.39 0.43 0.42 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Nuclear Periphery 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.65 0.22 0.15 0.28 0.18 – 0.11 0.11 0.11 0 0.05 0.61 0.49 0.39 0.39 0.36 0.48 0.69 0.69 0.58 0.72 0.58 0.48
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 – 1 0 1 1 1 1 1 1 2 0 0 0 0 0 0 1 0
Bud 1 0 0 0 1 0 – 6 3 7 0 6 0 2 2 0 2 0 0 0 1 0 2 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 – 2 3 2 2 9 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 98 36 7 47 111 76 – 189 106 104 60 100 56 130 202 62 59 79 7 2 7 0 6 6
Endoplasmic Reticulum 7 4 1 4 6 9 – 0 1 2 7 5 3 4 2 4 5 4 0 0 1 0 0 0
Endosome 10 3 0 1 13 0 – 1 0 0 0 0 14 54 44 18 16 21 47 27 8 5 54 77
Golgi 0 2 0 0 0 0 – 0 0 0 0 0 2 0 0 1 0 1 1 0 0 0 0 1
Mitochondria 25 5 8 9 26 54 – 119 70 59 37 68 3 2 1 3 4 4 4 1 4 0 4 7
Nucleus 3 2 0 0 0 1 – 1 2 0 0 2 1 3 1 0 2 0 6 3 0 0 3 12
Nuclear Periphery 7 5 1 0 1 3 – 0 1 0 0 0 1 1 0 0 0 0 15 3 1 0 4 2
Nucleolus 3 4 0 0 2 0 – 0 0 2 0 0 6 7 5 1 0 1 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 – 1 1 0 0 0 4 1 7 5 1 2 1 0 1 0 3 9
Vac/Vac Membrane 139 76 4 9 50 24 – 31 16 17 1 8 93 134 125 44 38 73 200 92 36 20 116 114
Unique Cell Count 247 117 18 60 181 130 286 149 150 87 162 153 276 317 114 105 153 290 135 63 28 201 240
Labelled Cell Count 293 138 21 70 213 168 351 203 194 108 200 184 340 391 140 128 186 290 135 63 28 201 240


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.5 4.6 5.7 6.1 4.6 – 4.4 6.3 4.9 4.4 4.7 8.2 8.8 7.8 8.7 8.6 8.4 – 6.6 7.0
Std Deviation (1e-4) 1.0 1.1 0.6 0.8 1.3 0.6 – 0.9 19.8 2.0 0.7 2.8 1.4 2.4 1.4 2.0 1.6 1.6 – 1.2 1.5
Intensity Change (Log2) – – – 0.32 0.43 0.02 – -0.04 0.45 0.09 -0.05 0.04 0.84 0.95 0.77 0.93 0.91 0.87 – 0.52 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.8 1.6 – 2.3 2.8 2.6 2.4 1.9 -0.2 0.7 2.1 1.2 1.4 1.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0.2 -0.4 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 3.1 2.2 1.5 1.3 1.2 2.1