Standard name
Description Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; genetic and comparative analysis suggests that translation begins at a non-canonical (Ile) start codon at -372 relative to the annotated start codon

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud 0 0 0.09 0.07 0.07 0 0.05 0.07 0.08 0.05 0.06 0.05 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.41 0.39 0.34 0.2 0.19 0.16 0.15 0.12 0.07 0.08 0.56 0.67 0.69 0 0.08 0 0.06 0.05 0
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.2 0.08 0.05 0 0.07 0 0 0 0 0 0 0.1 0.14 0 0.21 0.13 0.11 0.1 0.11 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.38 0.43 0.69 0.57 0.78 0.77 0.77 0.87 0.93 0.92 0.21 0.07 0.33 0.53 0.54 0.56 0.56 0.56 0.53
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.05 0 0.1 0 0.05 0 0 0 0 0.08 0.09 0 0.08 0 0.05 0 0 0.05
SpindlePole 0.2 0.08 0.16 0.06 0.19 0.1 0.15 0.12 0.05 0.09 0.06 0.12 0.07 0.06 0.05 0.06 0.09 0.08 0.07 0.06
Vac/Vac Membrane 0.13 0.13 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 1 0 0 1 0 0 1 0 0 0 2 2 1 4 17 13 14 26 24
Bud 6 12 30 21 25 15 31 41 31 11 19 19 12 28 0 2 3 4 5 9
Bud Neck 2 2 8 0 6 3 3 3 1 0 0 2 3 2 1 5 3 8 6 12
Bud Site 2 1 5 1 2 8 9 15 15 6 8 1 0 4
Cell Periphery 3 3 4 1 1 1 2 2 4 1 3 8 7 0 0 2 1 0 0 1
Cytoplasm 27 139 127 110 72 76 91 93 50 16 28 227 329 380 4 36 17 23 25 20
Endoplasmic Reticulum 10 5 1 4 3 3 3 4 3 3 3 2 6 2 0 1 3 3 3 5
Endosome 30 26 17 4 26 7 9 14 0 0 0 42 70 19 53 59 40 40 56 53
Golgi 2 0 0 0 0 0 0 0 0 0 1 1 0 0 5 6 4 2 7 7
Mitochondria 32 129 138 222 202 319 438 465 357 219 314 86 33 182 132 247 217 231 282 263
Nucleus 1 0 1 0 3 3 7 7 1 0 1 0 0 0 0 1 1 1 1 0
Nuclear Periphery 2 1 0 0 1 0 1 0 1 4 3 1 0 0 2 0 1 1 0 0
Nucleolus 0 0 1 1 1 1 0 5 3 2 3 0 0 1 0 0 1 1 1 1
Peroxisomes 3 24 15 8 35 4 26 23 6 1 1 34 46 9 20 15 19 16 19 26
SpindlePole 30 26 52 18 68 40 83 74 22 20 22 49 35 32 13 26 33 32 35 32
Vac/Vac Membrane 20 43 6 6 18 17 12 13 10 4 8 18 18 17 3 26 10 23 18 19
Unique Cell Count 149 337 323 320 356 410 569 603 409 235 340 409 492 552 250 458 386 417 503 494
Labelled Cell Count 180 412 405 396 464 497 715 760 504 287 414 492 561 677 250 458 386 417 503 494


Ambiguous, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 3.9 3.4 3.4 3.8 3.4 3.2 3.3 2.8 3.4 2.9 4.2 4.5 3.7 4.3 4.5 5.0
Std Deviation (1e-4) 0.7 0.6 1.3 1.1 1.4 1.1 1.1 1.1 0.6 5.5 3.2 1.0 1.2 1.0 1.1 1.3 1.6
Intensity Change (Log2) -0.0 0.14 -0.01 -0.09 -0.07 -0.3 -0.01 -0.22 0.3 0.38 0.1 0.34 0.38 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hfa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available