Standard name
Human Ortholog
Description Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; genetic and comparative analysis suggests that translation begins at a non-canonical (Ile) start codon at -372 relative to the annotated start codon

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud 0 0 0.09 0.07 0.07 0 0.05 0.07 0.08 0.05 0.06 0.05 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.41 0.39 0.34 0.2 0.19 0.16 0.15 0.12 0.07 0.08 0.56 0.67 0.69 0 0.08 0 0.06 0.05 0
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.2 0.08 0.05 0 0.07 0 0 0 0 0 0 0.1 0.14 0 0.21 0.13 0.11 0.1 0.11 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.38 0.43 0.69 0.57 0.78 0.77 0.77 0.87 0.93 0.92 0.21 0.07 0.33 0.53 0.54 0.56 0.56 0.56 0.53
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.05 0 0.1 0 0.05 0 0 0 0 0.08 0.09 0 0.08 0 0.05 0 0 0.05
SpindlePole 0.2 0.08 0.16 0.06 0.19 0.1 0.15 0.12 0.05 0.09 0.06 0.12 0.07 0.06 0.05 0.06 0.09 0.08 0.07 0.06
Vac/Vac Membrane 0.13 0.13 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 1 0 0 1 0 0 1 0 0 0 2 2 1 4 17 13 14 26 24
Bud 6 12 30 21 25 15 31 41 31 11 19 19 12 28 0 2 3 4 5 9
Bud Neck 2 2 8 0 6 3 3 3 1 0 0 2 3 2 1 5 3 8 6 12
Bud Site 2 1 5 1 2 8 9 15 15 6 8 1 0 4
Cell Periphery 3 3 4 1 1 1 2 2 4 1 3 8 7 0 0 2 1 0 0 1
Cytoplasm 27 139 127 110 72 76 91 93 50 16 28 227 329 380 4 36 17 23 25 20
Endoplasmic Reticulum 10 5 1 4 3 3 3 4 3 3 3 2 6 2 0 1 3 3 3 5
Endosome 30 26 17 4 26 7 9 14 0 0 0 42 70 19 53 59 40 40 56 53
Golgi 2 0 0 0 0 0 0 0 0 0 1 1 0 0 5 6 4 2 7 7
Mitochondria 32 129 138 222 202 319 438 465 357 219 314 86 33 182 132 247 217 231 282 263
Nucleus 1 0 1 0 3 3 7 7 1 0 1 0 0 0 0 1 1 1 1 0
Nuclear Periphery 2 1 0 0 1 0 1 0 1 4 3 1 0 0 2 0 1 1 0 0
Nucleolus 0 0 1 1 1 1 0 5 3 2 3 0 0 1 0 0 1 1 1 1
Peroxisomes 3 24 15 8 35 4 26 23 6 1 1 34 46 9 20 15 19 16 19 26
SpindlePole 30 26 52 18 68 40 83 74 22 20 22 49 35 32 13 26 33 32 35 32
Vac/Vac Membrane 20 43 6 6 18 17 12 13 10 4 8 18 18 17 3 26 10 23 18 19
Unique Cell Count 149 337 323 320 356 410 569 603 409 235 340 409 492 552 250 458 386 417 503 494
Labelled Cell Count 180 412 405 396 464 497 715 760 504 287 414 492 561 677 250 458 386 417 503 494


Ambiguous, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 3.9 3.4 3.4 3.8 3.4 3.2 3.3 2.8 3.4 2.9 4.2 4.5 3.7 4.3 4.5 5.0
Std Deviation (1e-4) 0.7 0.6 1.3 1.1 1.4 1.1 1.1 1.1 0.6 5.5 3.2 1.0 1.2 1.0 1.1 1.3 1.6
Intensity Change (Log2) -0.0 0.14 -0.01 -0.09 -0.07 -0.3 -0.01 -0.22 0.3 0.38 0.1 0.34 0.38 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9541 3.2532 3.1009 2.8412 3.3521 3.0748 0.1287 -1.1974 -0.8365 -1.064 -0.4616 -0.7448 3.9494 3.5988 3.1516 3.2504 3.5852 3.8345
Actin 0.0986 0.012 0.028 0.0088 0.0258 0.0237 0.0062 0.0187 0.0146 0.0048 0.0028 0.0184 0.0523 0.0295 0.0456 0.0163 0.0343 0.0153
Bud 0.0024 0.003 0.0038 0.0049 0.0052 0.0012 0.0015 0.003 0.0113 0.0381 0.0006 0.0056 0.0019 0.0071 0.0081 0.0071 0.003 0.0022
Bud Neck 0.0085 0.0032 0.0098 0.0083 0.0033 0.0287 0.0058 0.0026 0.0051 0.0018 0.0013 0.0074 0.0016 0.0057 0.0028 0.0031 0.0011 0.0085
Bud Periphery 0.0065 0.0062 0.0142 0.0137 0.0251 0.0037 0.0023 0.0047 0.0082 0.018 0.0018 0.0101 0.0047 0.0128 0.0113 0.0258 0.0133 0.0052
Bud Site 0.0135 0.0532 0.0343 0.0165 0.0124 0.004 0.0118 0.04 0.0538 0.0085 0.0013 0.0019 0.0056 0.0617 0.0314 0.0026 0.0022 0.0018
Cell Periphery 0.0074 0.0076 0.0106 0.0069 0.008 0.0111 0.0007 0.0022 0.0017 0.0026 0.001 0.0013 0.0042 0.0051 0.0019 0.0054 0.0017 0.0037
Cytoplasm 0.0887 0.2095 0.1882 0.0984 0.1423 0.1317 0.0149 0.0685 0.0819 0.0925 0.0354 0.0975 0.0778 0.1221 0.0682 0.1061 0.0862 0.1101
Cytoplasmic Foci 0.0928 0.1324 0.0973 0.079 0.1046 0.1188 0.145 0.1425 0.0922 0.1114 0.1668 0.1097 0.1671 0.143 0.1581 0.1107 0.1963 0.1689
Eisosomes 0.0022 0.0015 0.0018 0.0016 0.0019 0.001 0.0006 0.0009 0.0008 0.0005 0.0001 0.0004 0.0012 0.0016 0.0023 0.0003 0.0044 0.0007
Endoplasmic Reticulum 0.0308 0.02 0.0138 0.0088 0.0419 0.019 0.0044 0.0078 0.0064 0.0067 0.0045 0.0104 0.0054 0.0028 0.0035 0.005 0.0031 0.0039
Endosome 0.1588 0.1255 0.1026 0.074 0.1454 0.1269 0.1836 0.2234 0.1456 0.1665 0.1793 0.1563 0.1636 0.1665 0.1083 0.1349 0.1066 0.1233
Golgi 0.0521 0.0453 0.0431 0.0292 0.0724 0.0569 0.0538 0.0686 0.0703 0.0486 0.0543 0.0371 0.079 0.0744 0.0629 0.0635 0.0433 0.0618
Lipid Particles 0.0652 0.019 0.0279 0.023 0.036 0.0379 0.0812 0.0445 0.027 0.0618 0.0514 0.0349 0.051 0.01 0.0171 0.0081 0.0252 0.0294
Mitochondria 0.1355 0.113 0.2201 0.3882 0.2209 0.2514 0.277 0.1927 0.3011 0.2445 0.3105 0.2967 0.1622 0.1691 0.2766 0.3142 0.2343 0.1908
None 0.1342 0.1937 0.1444 0.1694 0.0773 0.1212 0.0623 0.1106 0.0953 0.1386 0.0217 0.1351 0.1079 0.1473 0.0941 0.1411 0.1242 0.205
Nuclear Periphery 0.0154 0.0025 0.0016 0.0045 0.0073 0.0025 0.03 0.0027 0.0135 0.0028 0.0731 0.003 0.0042 0.001 0.0083 0.0009 0.0004 0.0008
Nucleolus 0.0042 0.0009 0.0014 0.002 0.0071 0.001 0.0053 0.0019 0.0042 0.0029 0.0016 0.0036 0.001 0.0012 0.0011 0.0008 0.0004 0.0007
Nucleus 0.0073 0.0017 0.0022 0.0034 0.0094 0.0019 0.0304 0.002 0.0161 0.0035 0.0143 0.0072 0.0026 0.0023 0.0039 0.001 0.0006 0.0101
Peroxisomes 0.036 0.0289 0.036 0.028 0.0167 0.0355 0.0439 0.034 0.025 0.0218 0.0473 0.023 0.0793 0.0203 0.0822 0.0366 0.109 0.0467
Punctate Nuclear 0.0043 0.002 0.0025 0.0069 0.0163 0.0083 0.0128 0.0022 0.0079 0.0046 0.0029 0.0067 0.0164 0.0023 0.0028 0.0009 0.0011 0.0015
Vacuole 0.0289 0.0151 0.013 0.0185 0.0158 0.0098 0.0177 0.0205 0.0122 0.0134 0.0151 0.0245 0.0081 0.0113 0.0052 0.0115 0.0053 0.0067
Vacuole Periphery 0.0069 0.0038 0.0033 0.0059 0.0051 0.0037 0.0088 0.0059 0.0058 0.006 0.0128 0.0093 0.0028 0.003 0.0043 0.0042 0.0039 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0522 8.2043 9.0745 10.4934 11.7871 6.9227 10.291 11.3033 10.4495 12.5021
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
221 336 210 1257 1168 1450 1292 1040 1389 1786 1502 2297

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 633.98 744.23 947.18 797.50 602.83 715.46 782.68 711.89 607.79 720.87 805.68 758.74
Standard Deviation 65.94 99.47 109.74 108.28 78.24 94.77 92.43 110.45 77.26 96.33 110.85 117.28
Intensity Change Log 2 0.231311 0.579201 0.331047 0.247120 0.376671 0.239903 0.239038 0.484031 0.287342

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000721 0.001572 0.003102 0.008500 0.000950 0.001686 0.001362 0.000899 0.000914 0.001664 0.001605 0.005059
Bud Neck 0.061895 0.036617 0.009220 0.032180 0.045956 0.033752 0.025772 0.044577 0.048492 0.034291 0.023458 0.037793
Bud Site 0.012283 0.027627 0.031597 0.081084 0.016214 0.026791 0.018295 0.030455 0.015588 0.026949 0.020155 0.058161
Cell Periphery 0.000454 0.000257 0.000316 0.000262 0.000370 0.000156 0.000194 0.000329 0.000383 0.000175 0.000211 0.000292
Cytoplasm 0.193942* 0.051862 0.219918* 0.237139* 0.062120 0.046475 0.289911* 0.191873* 0.083094 0.047488 0.280125* 0.216644*
Cytoplasmic Foci 0.343429* 0.231179* 0.066185 0.138409 0.249637* 0.228895* 0.096151 0.153571 0.264560* 0.229325* 0.091962 0.145274
Eisosomes 0.000647 0.000237 0.000156 0.000149 0.000249 0.000175 0.000112 0.000177 0.000312 0.000187 0.000118 0.000162
Endoplasmic Reticulum 0.001148 0.001375 0.014282 0.003093 0.000867 0.000386 0.004885 0.002925 0.000912 0.000572 0.006199 0.003017
Endosome 0.057254 0.122763 0.023385 0.030662 0.093656 0.106234 0.014249 0.064842 0.087864 0.109343 0.015526 0.046137
Golgi 0.013782 0.090792 0.000679 0.011568 0.062967 0.112425* 0.002006 0.009976 0.055141 0.108355 0.001820 0.010847
Lipid Particles 0.047500 0.009740 0.010102 0.005274 0.028642 0.009172 0.009563 0.026087 0.031642 0.009279 0.009638 0.014698
Mitochondria 0.054715 0.177734* 0.000763 0.018391 0.276293* 0.244427* 0.009477 0.034451 0.241038* 0.231880* 0.008259 0.025662
Mitotic Spindle 0.002077 0.033061 0.006537 0.069599 0.008662 0.033782 0.026004 0.039424 0.007615 0.033646 0.023282 0.055936
None 0.001843 0.002109 0.002374 0.002313 0.002573 0.000991 0.006016 0.009263 0.002457 0.001201 0.005507 0.005459
Nuclear Periphery 0.000691 0.000276 0.003508 0.001374 0.000364 0.000219 0.003274 0.002729 0.000416 0.000230 0.003307 0.001987
Nuclear Periphery Foci 0.000353 0.000324 0.008252 0.001283 0.000505 0.000473 0.001807 0.002481 0.000480 0.000445 0.002708 0.001825
Nucleolus 0.004890 0.001579 0.002089 0.001923 0.004657 0.001784 0.005337 0.012626 0.004694 0.001745 0.004883 0.006769
Nucleus 0.093509 0.011632 0.390568* 0.098734 0.020814 0.008360 0.372639* 0.163846* 0.032381 0.008976 0.375145* 0.128214
Peroxisomes 0.060425 0.153284* 0.007731 0.074768 0.080637 0.105415* 0.025723 0.041116 0.077421 0.114420* 0.023207 0.059531
Vacuole 0.041895 0.036770 0.198278 0.179970 0.034106 0.031670 0.085603 0.160835 0.035346 0.032630 0.101357 0.171306
Vacuole Periphery 0.006546 0.009209 0.000958 0.003326 0.009763 0.006734 0.001620 0.007520 0.009251 0.007200 0.001527 0.005225

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.37 -2.63 -5.57 -4.75 -2.64 -2.41 -2.08 0.20 2.82 2.84 -2.91 -3.20 -5.33 -3.84 -4.00
Bud Neck 2.64 6.08 3.71 1.23 -9.28 3.08 5.53 1.07 -2.09 -4.76 3.87 7.35 3.85 -0.36 -5.32
Bud Site -3.28 -2.00 -9.86 -7.08 -4.74 -4.26 -0.61 -4.09 -0.46 -3.41 -5.19 -1.54 -11.60 -6.65 -9.46
Cell Periphery 2.25 2.16 4.00 0.57 2.76 3.09 2.65 1.03 -4.32 -3.59 3.47 3.01 2.22 -2.46 -1.57
Cytoplasm 9.16 -0.11 -2.69 -18.49 -2.47 3.53 -26.92 -15.76 -19.05 9.20 7.45 -24.05 -20.20 -29.79 6.43
Cytoplasmic Foci 6.10 17.50 14.55 10.46 -7.71 2.72 21.27 12.13 10.36 -8.98 4.90 25.98 20.07 17.15 -8.86
Eisosomes 4.07 5.44 5.76 1.77 1.72 3.71 8.55 4.50 -0.18 -7.68 5.26 9.63 7.86 1.80 -4.96
Endoplasmic Reticulum -0.39 -11.27 -6.56 -3.88 9.56 3.38 -13.33 -9.04 -12.33 6.13 2.12 -16.65 -12.23 -14.12 9.46
Endosome -5.53 4.76 4.72 10.53 -0.52 -2.33 19.68 6.68 9.27 -14.02 -4.29 20.09 12.34 17.39 -12.54
Golgi -9.30 5.53 2.62 10.51 -6.24 -9.28 18.91 15.57 23.15 -4.73 -11.34 19.19 16.36 25.98 -7.00
Lipid Particles 4.87 5.06 5.72 1.97 3.83 7.55 7.84 1.52 -6.84 -7.23 8.94 9.29 7.20 -3.30 -3.85
Mitochondria -7.12 5.52 4.31 11.72 -8.14 2.63 30.25 26.52 26.87 -6.67 0.81 29.53 27.54 31.19 -7.12
Mitotic Spindle -6.11 -2.24 -12.57 -3.97 -8.94 -8.26 -5.51 -7.34 -1.07 -2.56 -9.83 -5.87 -13.51 -4.30 -6.64
None -0.24 -0.45 -0.10 0.19 0.54 2.28 -2.84 -3.38 -4.49 -1.48 1.82 -2.90 -2.49 -3.98 0.29
Nuclear Periphery 2.18 -8.50 -5.72 -10.22 4.99 1.89 -11.55 -8.56 -8.99 1.76 2.62 -13.05 -12.21 -13.27 4.16
Nuclear Periphery Foci 0.24 -6.96 -7.22 -6.85 5.95 0.22 -7.28 -5.57 -5.11 -1.52 0.26 -9.85 -7.41 -6.13 3.07
Nucleolus 1.66 1.30 1.42 -1.09 0.48 3.23 -1.03 -5.09 -7.14 -4.74 3.67 -0.51 -2.41 -6.46 -2.31
Nucleus 7.56 -12.61 -1.96 -19.38 12.71 5.81 -41.37 -20.77 -23.30 19.28 9.00 -42.39 -22.49 -31.34 27.09
Peroxisomes -6.55 6.46 0.57 8.12 -12.13 -4.15 12.48 8.25 12.07 -4.10 -6.62 13.73 6.73 13.36 -8.44
Vacuole 0.21 -11.89 -23.58 -23.93 -1.33 0.87 -14.88 -21.11 -21.58 -9.96 0.79 -18.21 -34.23 -34.79 -15.01
Vacuole Periphery -1.24 4.39 3.34 4.25 -4.29 3.57 12.28 3.43 0.43 -6.68 2.59 12.99 7.45 4.96 -6.92