Standard name
Human Ortholog
Description Protein involved in telomeric silencing; required for quiescent cell telomere hypercluster localization at nuclear membrane vicinity; interacts with PAD4-domain of Sir4p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.16 0.14 0.14 0.12 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0.24 0 0 0 0.07 0 0 0 0 0 0 0 0.16 0.06 0 0.16 0.14 0.18 0.1 0.16 0.15 0.16 0.17 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.48 0.73 0.79 0.54 0.67 0.6 0.7 0.74 0.78 0.73 0.82 0.17 0.3 0.47 0.1 0.08 0.13 0 0.05 0.05 0.09 0 0.09
Nucleus 0.07 0.07 0 0.1 0.13 0.11 0.13 0.17 0.06 0.14 0.11 0.12 0 0.09 0.07 0.05 0 0.06 0 0 0 0.06 0 0
Nuclear Periphery 0.54 0.53 0.3 0.41 0.41 0.4 0.4 0.3 0.41 0.27 0.32 0.4 0.61 0.42 0.42 0.5 0.48 0.41 0.72 0.51 0.61 0.45 0.5 0.46
Nucleolus 0.24 0.2 0.13 0.16 0.11 0.18 0.19 0.15 0.24 0.19 0.21 0.2 0.27 0.12 0.15 0.25 0.18 0.2 0 0.09 0 0.05 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.05 0 0.05 0.06 0.06
Vac/Vac Membrane 0.19 0.17 0.08 0.05 0.08 0.07 0.06 0 0.05 0 0 0.06 0.2 0.15 0.1 0.26 0.31 0.26 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 2 2
Bud 0 1 3 1 1 5 7 4 3 9 6 6 0 0 0 0 0 1 2 1 1 0 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 2 3 4 9 14 2 9 4 3 0 0 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0
Cytoplasm 3 9 9 8 10 5 7 11 9 5 7 5 19 34 34 17 36 41 0 1 0 1 5 0
Endoplasmic Reticulum 0 1 0 0 0 0 1 0 1 1 0 0 0 0 1 6 15 12 0 1 1 1 1 0
Endosome 36 8 7 2 19 2 9 2 4 1 2 1 28 12 3 19 42 58 21 35 41 21 37 43
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 2 0 3 2 6
Mitochondria 27 100 187 164 151 233 249 294 236 258 165 175 29 62 113 12 22 43 6 12 15 12 8 21
Nucleus 10 14 11 21 37 39 53 73 20 46 24 26 6 18 17 6 10 18 2 10 5 7 6 2
Nuclear Periphery 80 110 78 85 116 137 164 125 131 90 73 85 105 87 100 61 140 135 148 116 172 58 110 104
Nucleolus 36 41 34 34 32 62 79 61 76 62 48 43 47 26 35 30 51 65 5 19 7 6 7 7
Peroxisomes 1 0 6 0 1 1 4 3 0 0 0 0 1 6 6 0 3 1 2 1 6 1 4 10
SpindlePole 3 6 5 4 4 3 6 6 3 2 4 0 3 10 15 2 7 12 8 10 11 6 13 13
Vac/Vac Membrane 29 35 21 10 23 24 25 10 15 11 6 13 34 31 24 31 89 85 1 8 7 6 6 7
Unique Cell Count 149 207 257 208 280 346 413 420 319 330 226 213 173 209 240 121 291 327 205 227 281 132 220 230
Labelled Cell Count 225 325 362 331 397 515 613 603 501 494 339 357 272 286 352 186 419 472 205 227 281 132 220 230


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.3 4.7 4.6 5.5 4.9 4.9 4.7 4.3 4.2 4.3 4.1 6.5 5.9 5.3 7.8 8.8 8.7 6.3 6.4 6.9
Std Deviation (1e-4) 1.1 1.1 1.0 1.1 1.2 0.9 1.1 1.0 1.2 1.0 1.2 1.2 1.7 1.6 1.4 1.5 1.9 2.1 1.5 1.9 1.7
Intensity Change (Log2) -0.02 0.21 0.07 0.07 -0.02 -0.13 -0.17 -0.12 -0.2 0.46 0.33 0.16 0.72 0.9 0.88 0.43 0.44 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0 -1.7 -1.5 -0.7 -0.5 -1.6 -0.2 -0.7 3.1 4.7 4.2 3.8 3.8 3.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.1
Endosome 0 2.2 0 -0.4 0 0 0 0 0 5.0 1.6 0 4.7 4.8 5.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.5 -1.4 -3.3 0 0 0 0 0 -11.4 -9.3 -5.8 -11.4 -15.7 -14.6
Nucleus 2.5 3.6 3.1 3.7 5.0 1.1 3.9 2.7 3.2 -0.4 1.9 1.4 0.3 -0.5 0.7
Nuclear Periphery 0 2.7 2.3 2.5 0 0 0 0 0 6.2 2.5 2.6 3.8 4.2 2.7
Nucleolus 0 -0.6 1.6 2.0 0 0 0 0 0 3.6 -0.3 0.4 2.8 1.4 2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0.4 0.1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.7 2.4 0 0.4 1.2
Vacuole -1.4 0 -0.6 -1.1 -3.5 -1.7 -2.6 -2.6 -0.9 3.5 2.3 0.7 4.6 6.5 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0758 3.2953 3.1536 3.0316 2.6398 3.1275 2.6814 3.6036 3.4366 2.6215 2.2004 3.0699 3.7309 4.1897 4.5399 4.0435 3.5163 3.9237
Actin 0.0473 0.0018 0.0101 0.013 0.0793 0.0096 0.0401 0.0031 0.0059 0.0058 0.0014 0.0086 0.0121 0.0074 0.007 0.0096 0.0006 0.0013
Bud 0.0005 0.0002 0.0002 0.0009 0.0013 0.0002 0.0002 0.0002 0.0003 0.0002 0 0 0.0007 0.0002 0.0002 0.0003 0 0
Bud Neck 0.0019 0.0008 0.0018 0.0271 0.0066 0.0013 0.0006 0.0002 0.0004 0.0006 0.0002 0.0007 0.0009 0.0004 0.0055 0.0004 0.0001 0.0003
Bud Periphery 0.0015 0.0004 0.0006 0.0018 0.0023 0.0004 0.0004 0.0006 0.0008 0.0006 0.0002 0.0001 0.0019 0.0006 0.0003 0.0014 0.0001 0.0002
Bud Site 0.002 0.0045 0.0045 0.0173 0.0071 0.0007 0.002 0.0008 0.0027 0.0007 0.0001 0.0001 0.0038 0.0013 0.0174 0.0003 0 0.0001
Cell Periphery 0.0006 0.0001 0.0002 0.0006 0.0007 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0002 0.0002 0 0
Cytoplasm 0.0037 0.0012 0.0012 0.0006 0.003 0.0003 0.0034 0.0004 0.0005 0.0004 0.0001 0.0002 0.0009 0.0094 0.0126 0.0009 0.0001 0.0004
Cytoplasmic Foci 0.0061 0.003 0.0029 0.0055 0.0046 0.0033 0.0175 0.0036 0.0063 0.0039 0.0032 0.0012 0.0039 0.0222 0.0121 0.0016 0.0056 0.0012
Eisosomes 0.0011 0.0001 0.0003 0.0004 0.0007 0.0001 0.0004 0.0001 0.0002 0.0003 0.0001 0 0.0005 0.0003 0.0002 0.0006 0.0001 0.0001
Endoplasmic Reticulum 0.0077 0.0022 0.0029 0.0023 0.0031 0.001 0.0018 0.0035 0.0016 0.0014 0.0013 0.0008 0.0036 0.0031 0.005 0.0028 0.0022 0.0013
Endosome 0.0235 0.0065 0.0056 0.0077 0.0175 0.0095 0.0281 0.0028 0.0101 0.0031 0.0034 0.0031 0.0206 0.0117 0.0153 0.0044 0.0017 0.0024
Golgi 0.0108 0.0018 0.003 0.0059 0.0141 0.0095 0.0085 0.0016 0.0085 0.0025 0.0042 0.0031 0.0054 0.0045 0.0056 0.0021 0.0038 0.0016
Lipid Particles 0.0399 0.0189 0.0329 0.0215 0.082 0.0261 0.0271 0.0255 0.0435 0.0237 0.122 0.0321 0.0243 0.0465 0.0194 0.0245 0.1373 0.0479
Mitochondria 0.0144 0.0019 0.0029 0.0115 0.0179 0.0138 0.0149 0.0022 0.0107 0.0023 0.0032 0.0019 0.0246 0.0066 0.0034 0.0039 0.0011 0.0007
None 0.0104 0.001 0.0042 0.0017 0.0133 0.0008 0.009 0.0014 0.0029 0.0029 0.0009 0.0003 0.0037 0.0052 0.0036 0.0131 0.0006 0.002
Nuclear Periphery 0.6847 0.7563 0.7239 0.7181 0.6063 0.8159 0.7686 0.8311 0.7852 0.7793 0.6878 0.8974 0.8005 0.6382 0.7329 0.7931 0.7152 0.8644
Nucleolus 0.012 0.0326 0.0212 0.0171 0.041 0.0175 0.0171 0.0352 0.0176 0.0267 0.0482 0.0057 0.0127 0.0204 0.0114 0.0106 0.0114 0.015
Nucleus 0.0414 0.0914 0.0953 0.0624 0.0313 0.0248 0.0287 0.0292 0.0287 0.0126 0.0158 0.0172 0.0142 0.0702 0.0675 0.024 0.004 0.0153
Peroxisomes 0.0036 0.0016 0.0038 0.0081 0.0032 0.004 0.0043 0.001 0.0052 0.0031 0.0015 0.0006 0.0042 0.0064 0.0075 0.0018 0.0016 0.0004
Punctate Nuclear 0.0648 0.0547 0.0611 0.0605 0.045 0.0503 0.0127 0.0374 0.0496 0.1172 0.0931 0.0176 0.0403 0.134 0.0509 0.0769 0.0998 0.0347
Vacuole 0.004 0.0023 0.0024 0.002 0.0074 0.0009 0.0015 0.0009 0.0023 0.0006 0.0007 0.0003 0.0031 0.0015 0.0012 0.0016 0.0003 0.0006
Vacuole Periphery 0.0181 0.0167 0.0189 0.0141 0.0123 0.0098 0.013 0.019 0.0169 0.0122 0.0126 0.0088 0.0176 0.0098 0.0208 0.0256 0.0145 0.0101

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7408 15.0622 12.0592 16.1833 17.8216 17.7262 16.5319 21.572 21.1794 21.1689
Translational Efficiency 1.6504 1.3329 1.5486 1.3892 1.1897 1.2914 1.165 0.9603 1.0982 1.1064

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
71 504 841 1238 1931 1972 373 1491 2002 2476 1214 2729

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 905.93 794.93 1092.43 974.53 745.40 840.59 1043.86 954.39 751.09 831.30 1077.51 963.53
Standard Deviation 1485.58 137.99 160.31 135.18 114.72 113.86 125.01 138.26 303.06 120.58 152.01 137.24
Intensity Change Log 2 -0.188572 0.270069 0.105307 0.173387 0.485842 0.356564 -0.013879 0.371479 0.224165

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000267 0.000501 0.000444 0.000199 0.000225 0.000191 0.000336 0.000169 0.000226 0.000254 0.000411 0.000183
Bud Neck 0.001401 0.000557 0.001875 0.001206 0.000340 0.000456 0.001051 0.001548 0.000378 0.000476 0.001622 0.001393
Bud Site 0.000416 0.000336 0.000188 0.000880 0.000198 0.000186 0.000197 0.002286 0.000206 0.000217 0.000191 0.001648
Cell Periphery 0.000108 0.000072 0.000025 0.000020 0.000053 0.000033 0.000019 0.000021 0.000055 0.000041 0.000023 0.000021
Cytoplasm 0.023390 0.004022 0.000920 0.001945 0.002728 0.001794 0.000316 0.002130 0.003461 0.002247 0.000734 0.002046
Cytoplasmic Foci 0.010264 0.004809 0.000264 0.001309 0.003729 0.004770 0.000121 0.000903 0.003961 0.004778 0.000220 0.001087
Eisosomes 0.000053 0.000025 0.000008 0.000004 0.000016 0.000012 0.000007 0.000005 0.000017 0.000015 0.000008 0.000004
Endoplasmic Reticulum 0.006525 0.006061 0.002930 0.002323 0.003470 0.003291 0.003265 0.001931 0.003578 0.003855 0.003033 0.002109
Endosome 0.008747 0.006922 0.000561 0.002413 0.003870 0.007883 0.000617 0.002243 0.004043 0.007687 0.000578 0.002320
Golgi 0.001159 0.003587 0.000220 0.001814 0.002376 0.003455 0.000103 0.001111 0.002333 0.003482 0.000184 0.001430
Lipid Particles 0.157773* 0.058332 0.000449 0.001162 0.055950 0.038844 0.001013 0.002261 0.059561 0.042811 0.000622 0.001762
Mitochondria 0.013950 0.008876 0.001021 0.001726 0.006414 0.007695 0.000695 0.001242 0.006681 0.007935 0.000921 0.001462
Mitotic Spindle 0.023541 0.054226 0.173408* 0.279013* 0.020686 0.053335 0.151126* 0.220963* 0.020787 0.053516 0.166562* 0.247297*
None 0.008739 0.006520 0.000519 0.000282 0.004829 0.001213 0.000268 0.000536 0.004968 0.002293 0.000442 0.000421
Nuclear Periphery 0.051906 0.045394 0.240826* 0.174929* 0.051630 0.066128 0.184163* 0.163631* 0.051640 0.061908 0.223416* 0.168756*
Nuclear Periphery Foci 0.574580* 0.744378* 0.448867* 0.508196* 0.795735* 0.757647* 0.590275* 0.570404* 0.787892* 0.754946* 0.492315* 0.542183*
Nucleolus 0.068488 0.023425 0.055230 0.007155 0.028419 0.025302 0.031161 0.009297 0.029840 0.024920 0.047835 0.008325
Nucleus 0.038806 0.027007 0.070616 0.009872 0.017290 0.021091 0.033786 0.012701 0.018054 0.022296 0.059300 0.011418
Peroxisomes 0.003881 0.002814 0.000516 0.000161 0.001020 0.001047 0.000270 0.000148 0.001122 0.001406 0.000440 0.000154
Vacuole 0.002568 0.001311 0.000636 0.003635 0.000476 0.003678 0.001000 0.004032 0.000550 0.003196 0.000748 0.003852
Vacuole Periphery 0.003436 0.000827 0.000478 0.001757 0.000547 0.001948 0.000210 0.002438 0.000649 0.001720 0.000396 0.002129

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.45 -2.37 0.60 3.69 4.42 0.76 -2.57 0.57 -0.25 2.98 -0.75 -4.69 0.39 1.49 5.94
Bud Neck 1.82 -1.19 0.01 -4.25 2.42 -1.65 -4.38 -6.07 -5.54 -0.06 -1.48 -9.91 -7.66 -7.16 1.64
Bud Site 0.75 1.12 -1.22 -1.72 -1.92 0.03 -1.73 -2.45 -2.46 -1.91 -0.18 -1.57 -2.93 -2.93 -2.59
Cell Periphery 1.32 3.31 3.52 5.38 1.67 3.87 3.25 5.98 2.79 0.74 2.62 5.57 6.97 5.70 1.63
Cytoplasm 1.78 1.95 1.87 1.11 -1.36 1.18 3.65 -0.30 -1.21 -2.88 1.66 3.13 1.07 -0.65 -2.56
Cytoplasmic Foci 1.09 1.83 1.72 2.61 -2.47 -0.77 5.62 4.31 5.98 -3.15 -0.63 5.70 4.50 6.20 -3.48
Eisosomes 2.22 3.72 4.09 5.93 3.87 2.60 1.64 7.73 6.04 2.11 1.35 4.79 9.49 8.93 4.08
Endoplasmic Reticulum 0.12 1.44 1.64 4.84 1.13 0.39 -0.85 4.35 2.95 3.44 -0.83 0.87 4.09 4.28 3.34
Endosome 0.71 3.38 2.57 3.04 -5.02 -3.69 5.69 2.68 5.32 -3.38 -3.82 7.28 3.17 6.05 -6.46
Golgi -2.46 4.13 -0.71 1.67 -2.11 -1.26 5.36 1.99 2.81 -1.79 -1.60 5.31 1.70 3.04 -2.71
Lipid Particles 3.47 5.69 5.66 9.30 -3.97 4.22 17.29 16.93 13.90 -1.94 4.16 18.33 17.94 16.85 -4.22
Mitochondria 1.02 3.24 3.03 2.78 -1.27 -0.99 9.26 8.09 5.95 -2.86 -1.04 9.46 7.87 6.29 -2.04
Mitotic Spindle -3.25 -13.59 -22.13 -18.73 -8.09 -7.87 -10.38 -24.48* -19.54 -4.31 -8.63 -19.22 -34.92 -28.11* -8.39
None 0.33 2.44 2.55 2.91 2.22 4.65 5.90 5.38 1.91 -0.65 2.91 6.01 6.09 3.76 0.59
Nuclear Periphery 0.52 -15.62 -11.16 -21.25 7.32 -5.29 -14.00 -23.96 -20.45 1.79 -4.08 -28.58 -30.62 -28.21 8.07
Nuclear Periphery Foci -3.93 2.57 1.30 14.22 -4.46 4.10 14.05 23.23* 18.94* 0.65 3.80 30.34 29.31 25.86* -5.04
Nucleolus 2.28 0.67 3.29 4.69 12.21 1.25 -0.58 7.93 7.09 5.19 1.76 -4.87 9.16 8.84 13.08
Nucleus 0.91 -3.16 2.61 3.88 12.24 -1.86 -4.36 1.39 3.06 4.87 -2.20 -11.17 2.97 5.14 12.84
Peroxisomes 0.81 3.44 3.75 3.57 3.62 0.12 3.76 5.25 4.18 1.60 -1.02 4.24 6.08 5.48 4.18
Vacuole 0.89 1.13 -1.40 -3.04 -3.39 -4.27 -2.09 -4.75 -1.91 -0.63 -4.36 -2.40 -6.11 -2.47 -3.23
Vacuole Periphery 1.23 1.40 0.75 -1.80 -2.60 -4.02 3.40 -2.97 -0.72 -3.43 -3.68 2.14 -3.52 -0.86 -4.15