Standard name
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.36 0.47 0.73 0.54 0.52 0.46 0.48 0.54 0.55 0.57 0.51 0.56 0.53 0.56 0.18 0.19 0.19 0.1 0.09 0.06 0.06 0.07 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.06 0.05 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.05 0.07 0.05 0.06 0.23 0.38 0.21 0.27 0.38 0.3 0.33 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Nucleus 0.08 0.06 0.08 0 0 0 0 0 0 0 0 0 0.11 0.1 0.11 0.11 0.06 0.07 0.06 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0 0.08 0.07 0.07 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.65 0.53 0.38 0.47 0.4 0.33 0.31 0.35 0.29 0.29 0.32 0.41 0.46 0.43 0.73 0.74 0.76 0.72 0.77 0.81 0.77 0.79 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 0 2 0 1 0 0 0 0 0 0 1 1 2 0 0 0
Bud 0 1 1 4 0 6 5 5 3 7 7 3 0 0 0 0 0 0 3 9 4 7 8 9
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 3 1 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 4 7 4 6 4 5 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 149 220 233 255 215 273 176 315 222 351 253 225 210 210 247 29 37 31 53 57 32 30 45 32
Endoplasmic Reticulum 2 2 0 1 2 6 9 28 1 8 4 10 2 0 5 9 8 5 4 3 3 4 8 6
Endosome 27 28 26 2 29 14 1 3 7 2 7 0 5 9 3 0 3 3 14 17 12 13 14 21
Golgi 2 0 2 0 1 1 0 6 2 1 1 0 0 0 0 0 2 1 3 3 2 1 0 0
Mitochondria 23 29 35 17 26 122 145 138 112 242 133 145 1 1 0 9 9 4 20 22 21 19 23 24
Nucleus 37 40 38 8 5 8 6 19 6 3 7 5 40 40 50 17 11 11 31 19 12 9 11 10
Nuclear Periphery 4 4 1 0 4 23 28 43 23 20 10 16 0 0 0 12 13 12 4 3 7 6 8 5
Nucleolus 0 3 1 0 2 4 0 6 0 1 0 6 0 0 0 1 0 0 0 2 0 2 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 3 1 0 2 2 1 0 0 0 0 1 1 0 0 4 5 3 6 9 7
Vac/Vac Membrane 300 404 263 134 187 208 126 203 145 187 130 143 154 183 193 115 146 122 375 514 465 369 530 482
Unique Cell Count 451 618 494 349 401 521 380 655 414 639 447 444 375 395 445 157 196 160 523 667 574 480 670 613
Labelled Cell Count 544 731 600 422 474 667 500 778 528 831 556 559 415 444 499 194 229 190 523 667 574 480 670 613


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.2 4.4 4.1 4.9 4.8 4.8 5.7 5.3 5.0 5.4 5.5 5.6 5.6 5.9 7.0 8.2 9.4 5.9 6.1 6.3
Std Deviation (1e-4) 0.8 1.4 1.1 0.9 1.8 2.1 2.0 2.3 2.4 2.4 2.4 2.6 0.9 0.9 1.0 1.7 2.2 2.7 1.5 1.8 1.8
Intensity Change (Log2) – – – -0.11 0.14 0.11 0.11 0.37 0.25 0.17 0.28 0.31 0.34 0.34 0.42 0.65 0.88 1.09 0.42 0.46 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.5 1.9 1.7 -0.2 0.3 1.9 2.6 2.9 1.1 2.6 1.8 2.6 -6.4 -6.9 -6.2
Endoplasmic Reticulum 0 0 0 0 4.7 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.2 0 0 -5.1 -2.9 -5.3 -3.1 -4.9 -3.1 -2.3 -4.1 -2.9 -2.2 -1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.4 0 0 6.6 8.1 12.0 9.1 9.9 -5.0 -5.1 -5.7 -0.6 -1.2 -2.1
Nucleus -3.4 -4.5 -4.7 -4.1 -3.7 -4.4 -6.5 -4.4 -4.8 1.5 1.3 1.9 1.2 -1.0 -0.3
Nuclear Periphery 0 0 0 0 5.6 5.0 3.6 2.9 3.9 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.3 -2.0 -4.3 -5.9 -7.6 -5.5 -8.2 -7.5 -6.5 -3.6 -2.0 -3.0 4.4 5.1 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0092 0.728 0.55 0.2583 1.0228 0.4835 1.8435 2.7527 2.8148 2.5786 2.3718 3.1535 2.2506 3.566 2.969 2.8609 3.7038 3.4123
Actin 0.0062 0.004 0.0002 0.0006 0.0012 0.0001 0.0002 0.0054 0.0001 0 0.0019 0.0003 0.0005 0.0005 0.0027 0 0.0005 0.0015
Bud 0.0023 0.0014 0.0018 0.0164 0.0023 0.0005 0.0005 0.001 0.0011 0.0005 0.0025 0.001 0.0017 0.0013 0.0031 0.0003 0.001 0.0009
Bud Neck 0.0082 0.0026 0.0024 0.0022 0.0012 0.003 0.0005 0.0034 0.001 0.0012 0.0039 0.0028 0.0057 0.0057 0.0011 0.001 0.0005 0.0026
Bud Periphery 0.0033 0.0018 0.0072 0.0167 0.0043 0.0007 0.001 0.0016 0.0013 0.0008 0.003 0.0011 0.0042 0.0029 0.0073 0.0006 0.0041 0.0034
Bud Site 0.008 0.0116 0.0042 0.0119 0.0036 0.0012 0.0007 0.0047 0.0022 0.0011 0.003 0.0005 0.0072 0.0098 0.0023 0.0006 0.0006 0.0006
Cell Periphery 0.0011 0.0011 0.0008 0.0011 0.0009 0.0007 0.0007 0.0011 0.001 0.0006 0.0008 0.0009 0.0015 0.0035 0.0014 0.0006 0.0006 0.0015
Cytoplasm 0.1037 0.1423 0.1148 0.1135 0.0839 0.1478 0.1371 0.1624 0.1158 0.1494 0.155 0.1736 0.1806 0.2188 0.1766 0.1228 0.1076 0.1918
Cytoplasmic Foci 0.0761 0.0438 0.0545 0.0954 0.0704 0.0565 0.0457 0.0354 0.0466 0.0621 0.0814 0.0535 0.0405 0.0333 0.0459 0.0509 0.0361 0.0521
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0093 0.0035 0.0039 0.0032 0.0047 0.0042 0.0054 0.0068 0.006 0.0022 0.0029 0.0032 0.0745 0.0826 0.0971 0.0038 0.1162 0.0855
Endosome 0.1572 0.0988 0.1086 0.1021 0.2715 0.1661 0.0976 0.0869 0.1059 0.1371 0.132 0.1302 0.1067 0.0661 0.112 0.178 0.2038 0.1354
Golgi 0.0051 0.0016 0.0015 0.003 0.0031 0.0016 0.0016 0.0015 0.0014 0.0014 0.0064 0.0019 0.002 0.0018 0.0024 0.0018 0.0024 0.0027
Lipid Particles 0.0356 0.0077 0.0113 0.0267 0.0068 0.0111 0.0096 0.0052 0.0082 0.0066 0.0045 0.0087 0.0106 0.0045 0.0137 0.0089 0.003 0.0087
Mitochondria 0.0066 0.0045 0.0053 0.0096 0.0037 0.0027 0.0039 0.0036 0.0034 0.0026 0.006 0.0039 0.0029 0.0035 0.0043 0.003 0.0033 0.0046
None 0.4345 0.4296 0.5051 0.4087 0.2783 0.3246 0.5364 0.4684 0.507 0.3939 0.4499 0.3994 0.414 0.4098 0.3617 0.3809 0.3512 0.2888
Nuclear Periphery 0.0101 0.005 0.0047 0.0068 0.0155 0.0059 0.0032 0.0033 0.0103 0.0018 0.0017 0.0027 0.0034 0.0039 0.0051 0.0025 0.0034 0.0042
Nucleolus 0.0042 0.0089 0.0052 0.0173 0.0047 0.0061 0.0015 0.0046 0.0028 0.0041 0.0015 0.0033 0.0016 0.0027 0.0017 0.0025 0.001 0.0017
Nucleus 0.0087 0.0242 0.0117 0.0257 0.0334 0.0167 0.0038 0.0106 0.0083 0.0065 0.0057 0.01 0.0051 0.0089 0.0057 0.0067 0.0021 0.005
Peroxisomes 0.0043 0.0013 0.002 0.0069 0.0013 0.0012 0.0008 0.0016 0.0076 0.0023 0.0017 0.0011 0.0007 0.0006 0.0012 0.0014 0.0008 0.0008
Punctate Nuclear 0.0059 0.0027 0.0064 0.0055 0.0019 0.0025 0.001 0.0011 0.0023 0.0017 0.0009 0.0016 0.0008 0.0009 0.0041 0.0006 0.0006 0.0009
Vacuole 0.1003 0.191 0.1348 0.1035 0.1857 0.2304 0.1379 0.1816 0.1556 0.2116 0.1275 0.1828 0.1268 0.1307 0.1379 0.2131 0.1442 0.1913
Vacuole Periphery 0.0092 0.0124 0.0137 0.0228 0.0215 0.0164 0.0105 0.0095 0.0117 0.0127 0.0075 0.0176 0.0089 0.0082 0.0126 0.0198 0.0169 0.0159

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.8841 31.9026 31.4031 36.479 31.634 32.0283 35.8773 37.544 29.4545 41.7801
Translational Efficiency 0.7386 0.9145 0.8845 0.6401 0.7951 0.9789 0.677 0.6628 0.9165 0.6954

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘