ORF
Human Ortholog
Description NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.91 0.93 0.9 0.82 0.83 0.8 0.86 0.78 0.98 0.99 0.99 0.99 1.0 0.98 0.92 0.96 0.96 0.93 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0.07 0 0.1 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 2 3 2 8 4 3 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 0 1 0 2 1 1 2 0 1 0 0 0 0 0 0 1 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 1 4 6 3 15 10 8 10 8 0 2 4 2 0 0 0 0 0 0 0 0
Cytoplasm 78 284 359 304 306 420 347 531 289 285 260 249 206 338 417 201 156 119 93 291 339 177 266 289
Endoplasmic Reticulum 0 5 2 1 8 19 11 66 26 19 6 6 3 1 3 7 5 5 0 1 1 0 1 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 1 3 4 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 0 0 1 1 1 6 0 14 11 5 17 0 0 0 2 1 0 0 1 0 0 0 0
Nucleus 0 0 2 0 2 3 13 12 2 1 6 5 2 2 3 0 1 0 1 4 1 4 7 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Nucleolus 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 2 11 19 9 16 43 11 34 18 39 0 3 4 4 0 3 1 1 1 0 2 4
Unique Cell Count 80 291 363 313 335 453 386 650 350 357 303 319 211 342 422 203 156 122 102 303 354 190 288 318
Labelled Cell Count 80 293 368 319 343 460 400 670 365 367 309 336 211 348 431 216 163 127 102 303 354 190 288 318


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 15.4 19.0 20.1 17.4 20.7 12.6 19.5 17.8 18.1 17.9 16.5 21.8 19.5 19.4 21.3 25.7 30.3 20.4 21.6 22.6
Std Deviation (1e-4) 4.8 3.5 3.2 3.8 4.3 4.0 3.5 3.8 3.6 3.7 3.5 3.5 4.9 4.4 3.7 5.0 5.6 9.0 6.2 5.6 6.3
Intensity Change (Log2) – – – 0.08 -0.13 0.13 -0.59 0.04 -0.09 -0.07 -0.08 -0.21 0.2 0.04 0.03 0.17 0.44 0.67 0.1 0.19 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.1 2.4 0 2.7 0 0 0 0 0 0 0
Cytoplasm -1.7 -4.7 -4.2 -5.3 -8.0 -7.6 -8.3 -6.6 -8.7 -1.2 -0.1 -0.1 0.1 1.3 -1.1
Endoplasmic Reticulum 0 0 3.3 2.4 5.9 4.7 3.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.8 3.4 0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 2.8 1.7 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 4.0 0 3.2 4.5 2.6 5.5 4.1 6.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.7122 21.1429 17.3765 17.231 14.2989 17.7609 17.9598 23.3656 21.346 20.5422 18.8453 21.1469 16.2655 20.7426 18.5368 17.0193 17.9137 19.0673
Actin 0.0264 0.0001 0.0052 0.0002 0.0524 0.0074 0.0271 0.0001 0.0014 0.0001 0.0026 0.0024 0.029 0.0002 0.0084 0.0043 0.0035 0.0011
Bud 0.0005 0 0.0004 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0005 0 0.0002 0.0013 0.0004 0.0002
Bud Neck 0.001 0.0001 0.0003 0.0002 0.0008 0.0008 0.0011 0.0001 0.0003 0.0004 0.0005 0.0008 0.0013 0.0001 0.0004 0.0013 0.001 0.0014
Bud Periphery 0.0004 0 0.0003 0 0.0006 0.0003 0.0003 0 0 0 0 0.0001 0.0005 0 0.0003 0.0019 0.0005 0.0001
Bud Site 0.0015 0 0.0003 0 0.0011 0.0001 0.0016 0.0001 0.0001 0 0 0.0001 0.0022 0.0002 0.0011 0.0024 0.0007 0.0001
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0003 0 0 0 0 0 0.0002 0 0.0001 0.0004 0.0003 0.0001
Cytoplasm 0.8344 0.9843 0.8856 0.9271 0.8469 0.8785 0.8445 0.987 0.9577 0.9668 0.9374 0.9484 0.8278 0.9873 0.9076 0.8551 0.8714 0.9148
Cytoplasmic Foci 0.0106 0.0002 0.014 0.0004 0.0178 0.0011 0.0126 0.0003 0.0012 0.0004 0.0075 0.0023 0.0153 0.0006 0.0089 0.0296 0.0158 0.0017
Eisosomes 0.0002 0 0.0001 0 0.0002 0.0001 0.0007 0 0 0 0 0 0.0002 0 0.0006 0 0.0001 0
Endoplasmic Reticulum 0.0044 0.0009 0.0028 0.0004 0.001 0.0046 0.007 0.0007 0.0017 0.0007 0.0016 0.0022 0.0077 0.0006 0.0054 0.0081 0.0243 0.0067
Endosome 0.0115 0.0001 0.0087 0.0001 0.0102 0.008 0.0158 0.0003 0.0012 0.0002 0.0099 0.0078 0.0212 0.0003 0.0108 0.0451 0.0311 0.002
Golgi 0.0033 0 0.0018 0 0.0089 0.0037 0.0047 0 0.0001 0 0.0024 0.0011 0.0073 0 0.0021 0.0086 0.0037 0.0001
Lipid Particles 0.0068 0 0.0027 0 0.0149 0.0035 0.0055 0 0 0 0.0006 0.0007 0.0042 0 0.0031 0.0026 0.0019 0.0002
Mitochondria 0.004 0 0.0043 0.0001 0.0038 0.0092 0.0057 0 0.0001 0 0.0009 0.0006 0.002 0 0.0089 0.0071 0.0034 0.0005
None 0.002 0.0002 0.0015 0.0005 0.0011 0.0006 0.005 0.0004 0.0008 0.0004 0.0007 0.0006 0.0165 0.0004 0.0077 0.0008 0.013 0.0009
Nuclear Periphery 0.0128 0.0014 0.0118 0.0045 0.0039 0.018 0.0125 0.0012 0.0024 0.0016 0.0038 0.0027 0.0151 0.0009 0.0043 0.004 0.0098 0.0107
Nucleolus 0.0006 0 0.0002 0 0.0001 0.0002 0.0004 0 0 0 0 0 0.0004 0 0.0002 0.0001 0.0005 0.0001
Nucleus 0.0645 0.0122 0.0536 0.0654 0.0175 0.0605 0.0357 0.0094 0.0318 0.0288 0.0295 0.0283 0.0372 0.0087 0.0236 0.0154 0.0117 0.0568
Peroxisomes 0.007 0 0.002 0 0.0129 0.0004 0.0054 0 0 0 0.0012 0.0001 0.0019 0 0.0025 0.0021 0.0008 0
Punctate Nuclear 0.0031 0.0001 0.003 0.0003 0.004 0.0012 0.0104 0.0001 0.0006 0.0003 0.0008 0.0008 0.0072 0.0001 0.0012 0.0023 0.001 0.0014
Vacuole 0.0038 0.0002 0.0009 0.0003 0.0009 0.0008 0.0019 0.0002 0.0004 0.0001 0.0004 0.0006 0.0015 0.0004 0.002 0.0058 0.0039 0.0008
Vacuole Periphery 0.001 0 0.0006 0.0001 0.0005 0.0009 0.0015 0 0.0001 0 0.0002 0.0003 0.0007 0 0.0007 0.0016 0.0013 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 192.1351 144.7667 143.8377 160.9032 150.3566 197.5153 178.005 192.9797 203.2157 191.1638
Translational Efficiency 2.1771 2.3024 1.829 1.5632 2.0483 2.4252 1.8401 1.4398 1.6693 1.7839

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 267 127 1564 1967 1874 1592 1865 2044 2141 1719 3429

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1824.07 1648.16 1671.92 1382.19 1345.54 1580.76 1655.65 1476.78 1363.57 1589.17 1656.85 1433.64
Standard Deviation 532.33 291.03 270.21 350.85 237.10 252.55 308.08 357.41 270.32 258.62 305.47 357.55
Intensity Change Log 2 — -0.146305 -0.125655 -0.400205 — 0.232433 0.299212 0.134270 — 0.026746 0.070164 -0.148810

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002082 0.000262 0.001072 0.001951 0.000072 0.000090 0.000268 0.000522 0.000147 0.000112 0.000328 0.001174
Bud Neck 0.011862 0.001044 0.007079 0.003982 0.000224 0.000496 0.002592 0.003172 0.000663 0.000564 0.002923 0.003541
Bud Site 0.046073 0.005237 0.009156 0.021888 0.000810 0.003817 0.002409 0.010789 0.002515 0.003994 0.002907 0.015852
Cell Periphery 0.007628 0.000134 0.000528 0.000139 0.000031 0.000028 0.000121 0.000090 0.000317 0.000042 0.000151 0.000112
Cytoplasm 0.544120* 0.841271* 0.468547* 0.663791* 0.860810* 0.882155* 0.652991* 0.752573* 0.848880* 0.877056* 0.639364* 0.712078*
Cytoplasmic Foci 0.040755 0.020087 0.017673 0.014571 0.006130 0.011777 0.005020 0.006319 0.007434 0.012814 0.005955 0.010083
Eisosomes 0.000041 0.000003 0.000018 0.000013 0.000001 0.000002 0.000005 0.000005 0.000002 0.000002 0.000006 0.000009
Endoplasmic Reticulum 0.007756 0.000215 0.001954 0.001765 0.000219 0.000162 0.000470 0.000309 0.000503 0.000168 0.000580 0.000973
Endosome 0.009571 0.001423 0.002393 0.015766 0.000145 0.000499 0.000963 0.002109 0.000501 0.000614 0.001068 0.008338
Golgi 0.035194 0.004587 0.000587 0.005176 0.000366 0.000778 0.000253 0.003065 0.001678 0.001253 0.000278 0.004028
Lipid Particles 0.024493 0.000477 0.002629 0.002949 0.000151 0.000238 0.000708 0.000360 0.001068 0.000268 0.000850 0.001541
Mitochondria 0.002901 0.000135 0.000517 0.005958 0.000119 0.000231 0.000293 0.000862 0.000224 0.000219 0.000309 0.003186
Mitotic Spindle 0.012160 0.003586 0.024542 0.060028 0.000548 0.001904 0.007002 0.041650 0.000986 0.002113 0.008297 0.050032
None 0.002676 0.010654 0.007112 0.015344 0.013013 0.009282 0.012961 0.014248 0.012624 0.009453 0.012529 0.014748
Nuclear Periphery 0.000555 0.000085 0.001711 0.001683 0.000141 0.000044 0.000508 0.000440 0.000156 0.000049 0.000597 0.001007
Nuclear Periphery Foci 0.030911 0.004045 0.004795 0.003989 0.001315 0.000947 0.002324 0.001306 0.002430 0.001333 0.002506 0.002530
Nucleolus 0.010506 0.000091 0.000306 0.000634 0.000112 0.000085 0.000217 0.000412 0.000504 0.000086 0.000224 0.000513
Nucleus 0.153479* 0.092480 0.356099* 0.089632 0.111000 0.077527 0.283784* 0.124543 0.112600 0.079392 0.289126* 0.108619
Peroxisomes 0.002267 0.000083 0.000230 0.003642 0.000022 0.000767 0.000169 0.000645 0.000106 0.000682 0.000174 0.002012
Vacuole 0.044064 0.013000 0.092722 0.081815 0.004395 0.008705 0.026850 0.035148 0.005890 0.009240 0.031716 0.056433
Vacuole Periphery 0.010908 0.001100 0.000328 0.005285 0.000375 0.000465 0.000093 0.001433 0.000772 0.000544 0.000111 0.003190

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.53 2.16 3.67 -1.08 2.76 -1.36 -14.35 -3.46 -3.16 1.34 1.37 -9.83 -2.68 -3.06 0.45
Bud Neck 3.72 1.51 3.31 -4.71 4.65 -3.57 -7.76 -9.06 -7.87 0.72 0.87 -7.20 -8.80 -11.34 1.92
Bud Site 3.26 2.82 3.01 -2.23 1.31 -3.98 -11.98 -8.89 -3.59 -4.52 -1.75 -3.35 -7.10 -4.88 -5.82
Cell Periphery 4.42 4.07 4.45 3.08 4.70 0.85 -17.71 -13.01 -14.08 9.16 3.83 1.18 3.01 -11.59 9.29
Cytoplasm -7.67 4.28 -2.02 17.72 -11.94 -3.59 47.29 34.09 36.16 -11.52 -4.55 46.28 40.36 46.38 -11.96
Cytoplasmic Foci 2.87 1.91 5.32 5.69 3.38 -7.31 -2.08 -2.43 5.93 -0.28 -7.39 -1.90 -2.16 6.47 0.11
Eisosomes 3.10 1.21 2.49 -9.78 5.64 -4.66 -15.10 -21.44 -14.97 -0.40 0.05 -10.34 -13.10 -19.74 -0.53
Endoplasmic Reticulum 2.67 1.86 2.54 -1.89 3.97 1.15 -6.60 -2.38 -3.37 5.17 2.75 -1.66 0.35 -3.27 2.79
Endosome 2.95 2.44 2.09 -2.32 -0.89 -2.95 -5.03 -4.63 -2.41 -0.76 -0.77 -3.04 -4.61 -4.22 -3.18
Golgi 3.02 3.45 3.41 1.11 -1.04 -1.30 1.31 -0.85 -0.21 -1.08 0.91 3.41 1.21 0.28 -1.95
Lipid Particles 2.97 2.49 2.84 -3.51 3.86 -1.36 -14.22 -12.57 -8.10 4.28 2.33 -1.69 -0.70 -7.37 2.12
Mitochondria 1.82 1.44 0.98 -2.72 -1.42 -0.78 -6.68 -2.76 -1.37 -0.14 0.18 -2.87 -2.79 -2.75 -1.96
Mitotic Spindle 1.59 -0.49 -2.38 -8.19 -2.30 -2.31 -7.92 -10.13 -9.20 -5.82 -1.81 -8.02 -14.10 -12.94 -7.93
None -2.45 -2.40 -5.74 0.35 -3.01 3.61 2.23 3.36 -0.38 2.03 3.33 2.37 3.63 0.21 2.32
Nuclear Periphery 3.59 -7.71 -1.94 -5.98 6.17 0.95 -7.16 -5.30 -22.33 4.69 1.06 -8.30 -5.52 -11.85 3.04
Nuclear Periphery Foci 3.09 2.99 3.55 3.32 5.41 1.65 -6.42 -1.38 -3.92 6.05 2.21 -2.35 1.78 -0.95 6.54
Nucleolus 2.33 2.27 2.25 -5.05 -0.87 0.65 -2.78 -3.46 -5.68 -1.64 2.35 1.29 0.66 -7.61 -2.58
Nucleus 2.79 -5.51 1.96 -2.99 10.84 9.51 -35.48 -12.24 -21.10 24.92 9.35 -36.06 -8.78 -20.06 31.29
Peroxisomes 1.54 1.43 1.00 -2.26 -1.76 -1.82 -1.97 -2.47 1.27 -1.37 -1.58 -0.10 -2.42 0.43 -2.45
Vacuole 2.38 -7.62 -14.03 -25.11 0.56 -8.27 -21.16 -28.16 -26.08 -7.71 -7.53 -22.54 -41.05 -38.54 -12.72
Vacuole Periphery 2.80 3.02 2.76 -0.31 -2.64 -0.11 1.42 -0.15 -0.02 -2.00 0.86 2.71 0.08 -0.94 -3.79
ORF
Human Ortholog
Description NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YMR226C

YMR226C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr226c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available