ORF
Description NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.91 0.93 0.9 0.82 0.83 0.8 0.86 0.78 0.98 0.99 0.99 0.99 1.0 0.98 0.92 0.96 0.96 0.93 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0.07 0 0.1 0.06 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 2 3 2 8 4 3 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 0 1 0 2 1 1 2 0 1 0 0 0 0 0 0 1 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 2 1 4 6 3 15 10 8 10 8 0 2 4 2 0 0 0 0 0 0 0 0
Cytoplasm 78 284 359 304 306 420 347 531 289 285 260 249 206 338 417 201 156 119 93 291 339 177 266 289
Endoplasmic Reticulum 0 5 2 1 8 19 11 66 26 19 6 6 3 1 3 7 5 5 0 1 1 0 1 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 1 3 4 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 0 0 1 1 1 6 0 14 11 5 17 0 0 0 2 1 0 0 1 0 0 0 0
Nucleus 0 0 2 0 2 3 13 12 2 1 6 5 2 2 3 0 1 0 1 4 1 4 7 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Nucleolus 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 2 11 19 9 16 43 11 34 18 39 0 3 4 4 0 3 1 1 1 0 2 4
Unique Cell Count 80 291 363 313 335 453 386 650 350 357 303 319 211 342 422 203 156 122 102 303 354 190 288 318
Labelled Cell Count 80 293 368 319 343 460 400 670 365 367 309 336 211 348 431 216 163 127 102 303 354 190 288 318


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 15.4 19.0 20.1 17.4 20.7 12.6 19.5 17.8 18.1 17.9 16.5 21.8 19.5 19.4 21.3 25.7 30.3 20.4 21.6 22.6
Std Deviation (1e-4) 4.8 3.5 3.2 3.8 4.3 4.0 3.5 3.8 3.6 3.7 3.5 3.5 4.9 4.4 3.7 5.0 5.6 9.0 6.2 5.6 6.3
Intensity Change (Log2) 0.08 -0.13 0.13 -0.59 0.04 -0.09 -0.07 -0.08 -0.21 0.2 0.04 0.03 0.17 0.44 0.67 0.1 0.19 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.1 2.4 0 2.7 0 0 0 0 0 0 0
Cytoplasm -1.7 -4.7 -4.2 -5.3 -8.0 -7.6 -8.3 -6.6 -8.7 -1.2 -0.1 -0.1 0.1 1.3 -1.1
Endoplasmic Reticulum 0 0 3.3 2.4 5.9 4.7 3.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.8 3.4 0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 2.8 1.7 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 4.0 0 3.2 4.5 2.6 5.5 4.1 6.4 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr226c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available