Standard name
Description Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.94 0.96 0.96 0.96 0.9 0.89 0.82 0.79 0.72 0.79 0.99 0.98 1.0 0.96 0.92 0.69 0.79 0.61 0.84 0.81 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.09 0.17 0.15 0.38 0.27 0.45 0.32 0 0 0 0 0 0.16 0.1 0.16 0.08 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 1 3 5
Bud 0 2 0 0 0 0 3 1 4 8 3 1 0 1 0 0 0 1 0 3 1 4 5
Bud Neck 1 1 4 3 3 5 3 11 4 14 3 2 5 0 1 0 1 0 1 1 0 1 2
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 1 2 2 1 1 0 0 0 0 0 0 0 3 2 1 1 1 0 1 2
Cytoplasm 191 202 103 115 192 191 250 193 141 190 123 148 211 200 246 297 193 135 168 67 109 168 203
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 22 14 2 0 0 0 0 3
Endosome 0 1 0 1 1 0 0 1 0 0 0 0 0 1 0 1 4 2 2 1 0 0 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 1
Mitochondria 24 3 4 4 3 17 47 32 65 65 76 61 1 0 0 7 4 30 21 17 10 14 12
Nucleus 0 0 0 0 2 0 4 2 2 3 0 1 0 0 0 0 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 3 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 1 6 6 0 4 2 4 1 0 0 1 1 3 0 6 0 3 3
Vac/Vac Membrane 1 4 0 0 1 1 5 6 1 0 0 4 0 2 1 11 9 3 10 4 1 2 3
Unique Cell Count 205 208 109 120 199 199 278 217 172 241 170 188 213 204 247 310 209 196 214 110 131 208 251
Labelled Cell Count 219 213 113 126 206 217 323 253 218 284 213 221 218 204 248 343 230 196 214 110 131 208 251


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.9 4.9 4.5 4.7 4.0 3.5 4.2 3.1 3.2 3.0 3.4 4.1 4.9 4.7 5.0 5.2 4.7 4.4 4.6 4.8
Std Deviation (1e-4) 0.7 0.9 2.2 2.4 2.2 2.1 1.8 2.3 1.8 1.3 1.4 1.3 0.8 1.8 1.5 1.0 1.3 0.9 1.6 1.4 1.6
Intensity Change (Log2) -0.11 -0.06 -0.29 -0.49 -0.23 -0.65 -0.59 -0.71 -0.54 -0.24 0.01 -0.05 0.05 0.09 -0.05 -0.15 -0.07 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available