Standard name
Description Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.22 0.33 0.22 0.24 0.07 0.15 0.14 0.1 0.1 0.13 0.18 0.07 0 0 0.08 0.07 0 0.1 0.09 0.12
Bud 0 0 0 0 0.06 0.06 0.07 0.05 0.06 0.06 0.07 0.07 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.06 0.05 0 0 0 0 0 0.05 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.16 0.06 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0 0 0
Endosome 0.12 0.09 0.12 0.12 0.15 0.22 0.17 0.18 0.11 0.13 0.11 0.22 0.05 0.14 0.14 0.19 0.21 0.26 0.14 0.1 0.09
Golgi 0.6 0.5 0.61 0.66 0.43 0.25 0.24 0.28 0.14 0.11 0.11 0.19 0.59 0.66 0.66 0.61 0.55 0.57 0.62 0.66 0.67
Mitochondria 0.53 0.39 0.15 0.3 0.35 0.55 0.62 0.36 0.75 0.7 0.62 0.4 0.41 0.18 0.29 0 0.06 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.06 0.1 0.06 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08
SpindlePole 0 0 0.06 0.05 0.1 0.06 0.07 0.09 0 0.06 0.05 0.1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.07 0 0 0.07 0 0.09 0 0 0.06 0.08 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 73 73 156 79 92 39 90 70 56 60 76 117 19 11 13 31 26 23 22 26 34
Bud 11 7 16 11 22 34 42 24 34 39 42 49 14 9 6 0 2 0 1 1 0
Bud Neck 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 3 2 5 7 5 2 0 0 0
Cell Periphery 3 6 1 5 2 5 2 2 5 1 4 8 2 3 1 0 0 0 0 0 0
Cytoplasm 8 23 20 22 19 13 10 14 15 22 29 17 14 16 18 0 5 2 2 0 1
Endoplasmic Reticulum 14 52 29 13 14 14 21 7 17 11 36 40 21 12 11 2 5 2 1 3 1
Endosome 40 30 56 44 58 121 97 90 59 77 66 145 15 49 43 77 81 159 33 29 26
Golgi 200 164 293 238 163 136 138 138 73 68 65 127 161 228 207 244 212 345 141 197 200
Mitochondria 177 127 74 106 132 300 358 174 403 428 366 267 111 63 91 11 22 29 9 12 6
Nucleus 0 0 0 1 1 1 1 2 2 2 2 0 0 1 0 1 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 2 6 3 8 4 5 6 1 1 1 1 0 1 0 0 0
Nucleolus 3 2 4 1 8 14 34 50 31 32 40 51 0 11 9 0 0 0 0 0 0
Peroxisomes 1 0 2 1 5 29 13 7 10 9 6 17 3 4 5 15 8 23 9 21 24
SpindlePole 4 2 29 19 40 34 43 43 18 34 28 64 1 9 3 3 4 5 3 1 0
Vac/Vac Membrane 13 23 36 14 14 38 21 42 18 27 38 52 12 21 14 6 14 7 2 0 0
Unique Cell Count 333 329 480 358 382 544 581 488 539 612 594 664 273 348 312 401 387 605 230 299 297
Labelled Cell Count 547 509 717 555 571 782 879 668 754 821 809 962 374 438 422 401 387 605 230 299 297


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 10.2 10.1 9.6 9.3 8.8 8.5 8.9 8.0 7.9 7.7 8.5 11.6 12.0 12.1 9.7 10.2 10.5
Std Deviation (1e-4) 1.7 1.7 1.2 1.2 1.5 1.3 1.2 1.4 1.4 1.5 1.4 1.5 1.7 1.7 1.9 1.6 1.4 1.6
Intensity Change (Log2) -0.06 -0.12 -0.2 -0.24 -0.18 -0.33 -0.36 -0.38 -0.24 0.21 0.26 0.26 -0.05 0.02 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.3 -2.7 -10.3 -6.5 -6.7 -8.7 -9.3 -7.8 -5.8 -8.0 -10.4 -9.5
Bud -0.2 1.7 2.2 2.8 1.2 2.2 2.3 2.7 2.9 1.2 -0.6 -1.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.6 -1.6 -2.4 -1.1 -1.2 -0.5 0.6 -1.5 0.6 0.3 1.0
Endoplasmic Reticulum -1.6 -1.6 -2.8 -1.9 -3.8 -2.2 -3.7 0 0 0.9 -1.7 -1.6
Endosome 0.3 1.5 4.5 2.3 2.9 -0.4 0.5 -0.3 4.5 -2.8 1.0 0.9
Golgi 1.6 -5.4 -11.7 -12.3 -10.3 -15.8 -17.4 -17.3 -14.5 -0.6 1.3 1.5
Mitochondria 4.9 6.5 13.2 15.2 7.2 19.0 17.9 15.3 9.0 7.7 1.0 4.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.6 2.1 4.4 6.4 4.3 4.0 4.9 5.3 0 2.5 2.2
Peroxisomes 0 0 4.6 2.5 0 2.1 0 0 2.8 0 0 0
SpindlePole -0.5 2.4 0.1 0.9 1.6 -2.1 -0.3 -1.0 2.2 -3.8 -2.3 -3.5
Vacuole -2.2 -2.4 -0.3 -2.8 0.6 -3.0 -2.2 -0.7 0.2 -1.7 -0.8 -1.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available