Standard name
Description Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.12 0.15 0.08 0.15 0.12 0.13 0.13 0.13 0.15 0 0 0 0.12 0.1 0.06 0 0.06 0 0 0 0
Bud 0.06 0.05 0 0 0 0.05 0.06 0.06 0.07 0.08 0.08 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.17 0.09 0.17 0.17 0.12 0.14 0.11 0.11 0.11 0.1 0.15 0.22 0.22 0.26 0.31 0.22 0.12 0 0 0 0.06 0.13 0.14
Cytoplasm 0.13 0.34 0.11 0.12 0.17 0.19 0.31 0.43 0.49 0.55 0.59 0.16 0.16 0.18 0.44 0.54 0.65 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.67 0.61 0.81 0.82 0.78 0.73 0.59 0.4 0.35 0.22 0.26 0.72 0.72 0.74 0 0 0.12 0.82 0.76 0.9 0.82 0.75 0.74
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.19 0.08 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 17 43 37 25 62 51 53 46 38 49 11 13 9 2 6 1 2 15 12 10 17 21
Bud 5 13 5 6 5 21 24 27 25 23 25 2 2 2 0 4 1 1 2 1 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 7 3 4 7 11
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 15 22 60 41 37 58 47 46 39 31 48 65 65 81 5 13 2 2 6 8 21 58 91
Cytoplasm 12 81 37 28 52 77 129 179 178 164 189 48 48 55 7 32 11 4 17 3 13 8 13
Endoplasmic Reticulum 60 146 286 200 243 297 246 167 127 65 84 214 214 231 0 0 2 96 200 330 303 341 478
Endosome 0 0 1 0 0 1 4 9 4 7 2 0 0 0 0 0 0 0 1 1 1 0 0
Golgi 2 0 2 0 6 2 3 0 1 1 2 0 0 0 0 1 0 0 2 0 0 0 1
Mitochondria 0 2 1 3 2 2 0 1 5 2 1 0 0 0 1 2 1 1 1 2 3 4 4
Nucleus 0 0 0 0 0 0 0 0 0 0 2 2 2 2 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 3 1 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 1 1 4 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 2 0 4 12 4 14 14 15 16 10 1 1 6 3 5 1 2 4 0 8 7 14
Unique Cell Count 89 239 351 243 313 405 420 418 364 296 320 298 296 311 16 59 17 118 262 368 372 453 650
Labelled Cell Count 99 283 435 320 384 528 521 502 445 350 415 343 345 386 20 66 19 118 262 368 372 453 650


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.7 13.9 14.5 15.8 20.6 20.0 19.5 17.9 16.2 15.0 15.5 13.9 13.9 14.5 16.3 19.3 22.5 15.6 18.1 20.1
Std Deviation (1e-4) 2.9 2.9 2.8 3.0 4.1 4.1 3.5 3.5 3.6 3.0 2.9 2.0 2.0 2.1 4.7 7.0 8.3 2.6 3.5 4.2
Intensity Change (Log2) 0.13 0.51 0.47 0.43 0.31 0.16 0.05 0.1 -0.06 -0.06 0.0 0.18 0.42 0.63 0.11 0.32 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.8 1.2 0 0.2 0.2 0.2 1.2 -3.9 -3.5 -4.5 0 -0.5 0
Bud 0 0 2.8 3.1 3.5 3.6 4.0 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.9 -1.0 -2.4 -2.4 -2.5 -2.4 -0.7 1.5 1.6 2.8 0 0.9 0
Cytoplasm 0.4 2.3 3.2 6.8 9.9 11.2 12.3 13.3 2.1 2.1 2.7 0 8.3 0
Endoplasmic Reticulum 0.3 -1.2 -2.7 -6.8 -11.7 -12.6 -15.1 -14.4 -2.9 -2.8 -2.2 -7.7 -12.6 -6.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 0 3.5 3.5 3.8 4.4 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dfg5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available