Standard name
Description Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.16 0.12 0.13 0.11 0.15 0.06 0.06 0.08 0.13 0.12 0.06 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.25 0.15 0.21 0.59 0.44 0.54 0.47 0.61 0.55 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0.79 0.79 0.51 0.51 0.51 0.51 0.33 0.42 0.48 0.42 0.46 0.4 0.39 0.31 0.31 0.61 0.62 0.61 0.59 0.61 0.37 0.33 0.22 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.23 0.54 0.62 0.46 0.33 0.33 0.26 0.27 0.24 0.27 0.24 0.6 0.58 0.59 0.34 0.26 0.32 0.34 0.29 0.45 0.41 0.35 0.31
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0.05 0 0.05 0.06 0.15 0.22 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 1 0 0 1 0 0 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 1 1 1 1 2 0 4 7 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 0 4 2 14 11 21 10 12 7 7 23 40 41 26 23 14 1 1 0 3 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 10 8 7 0 0 0 1 1 2
Endosome 0 0 0 0 0 1 1 1 0 0 0 0 5 14 15 5 8 2 2 1 2 3 16 16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 1 1
Mitochondria 7 2 5 20 6 19 56 71 48 37 71 61 6 9 3 14 6 6 0 2 1 2 7 13
Nucleus 128 99 21 41 20 45 31 67 43 33 53 44 116 98 112 274 251 160 101 89 24 63 59 56
Nuclear Periphery 2 0 0 0 1 0 4 1 2 5 2 3 1 4 1 4 5 0 0 1 0 1 5 8
Nucleolus 46 29 22 50 18 29 31 41 24 19 31 27 177 183 210 152 105 85 57 41 29 80 95 87
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 5 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 1 0 1 7 0 1 1 1 3 10 10 6 6 4 0 0 2 4 13 8
Vac/Vac Membrane 3 4 1 2 1 2 2 7 2 1 0 6 1 7 8 21 34 12 5 7 4 29 60 73
Unique Cell Count 163 125 41 80 39 89 95 160 89 78 116 111 295 318 356 451 405 264 172 147 66 196 271 280
Labelled Cell Count 188 135 49 119 50 111 139 218 133 109 169 156 333 371 406 515 446 290 172 147 66 196 271 280


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.6 6.8 6.4 5.6 5.7 4.3 5.0 4.6 4.6 4.4 4.7 8.3 7.8 7.7 10.6 12.2 12.1 7.3 7.6 8.1
Std Deviation (1e-4) 1.4 2.2 1.5 1.6 1.3 1.4 1.2 1.6 1.0 1.6 1.2 1.7 2.1 2.2 2.0 4.3 4.0 4.7 1.3 1.5 1.4
Intensity Change (Log2) -0.07 -0.28 -0.25 -0.65 -0.45 -0.55 -0.57 -0.61 -0.51 0.3 0.21 0.18 0.65 0.85 0.84 0.11 0.17 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 0.4 1.2 0 3.8 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 -0.1 -2.0 -1.1 -0.3 -0.9 -0.6 -1.3 -1.5 -2.6 -2.5 1.2 1.3 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 -0.7 -2.3 0 -3.5 0 0 0 0 0.8 0.5 0.7 -2.6 -3.7 -2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0