Standard name
Human Ortholog
Description Protein required for assembly of U2 snRNP into the spliceosome; forms a complex with Hsh49p and Hsh155p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.1 0.07 0 0 0 0 0 0 0 0.14 0.18 0.18 0.14 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0 0 0.19 0.33 0.38 0.32 0.3 0.47 0.44 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.91 0.87 0.86 0.88 0.84 0.85 0.84 0.83 0.76 0.81 0.85 0.79 0.81 0.82 0.86 0.84 0.87 0.88 0.84 0.83 0.78 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.05 0.06 0.05 0.07 0.1 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.12 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
Bud 0 0 1 1 0 7 14 12 4 8 0 4 0 0 0 0 0 0 0 4 3 3 2 5
Bud Neck 0 0 0 0 1 0 0 2 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 4 0 3 13 23 20 14 26 13 15 0 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 5 8 20 15 18 16 21 22 12 11 11 6 46 62 105 44 34 37 0 1 0 2 5 3
Endoplasmic Reticulum 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 6 9 5 2 0 3 1 2 1
Endosome 0 0 1 2 0 0 0 0 1 0 0 0 0 4 2 0 2 1 2 6 5 5 2 5
Golgi 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 2 2 1 2 4 1 2 1
Mitochondria 35 17 11 5 46 157 227 178 133 272 139 210 0 0 2 10 8 12 0 3 1 4 6 12
Nucleus 167 418 344 125 209 416 495 474 372 483 241 330 290 272 483 258 286 276 153 411 328 366 338 398
Nuclear Periphery 2 1 9 0 1 7 11 4 7 7 5 5 0 0 0 0 3 0 0 1 0 0 1 2
Nucleolus 9 8 13 1 1 21 31 34 24 42 32 26 0 0 2 3 3 0 2 12 18 8 9 33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 8 0 3 1 0 3 1 2 0 3 2 1 5 2 4 1 0 1 4 4 4 6
Vac/Vac Membrane 2 3 2 3 3 3 1 6 4 4 3 1 2 7 6 10 6 12 8 16 16 37 51 67
Unique Cell Count 177 439 376 144 244 473 591 559 444 580 316 408 340 344 593 313 332 327 177 466 391 439 432 545
Labelled Cell Count 220 458 414 152 285 641 823 755 575 857 445 600 341 346 609 334 358 346 177 466 391 439 432 545


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 6.2 5.9 5.7 5.0 4.2 4.2 4.2 4.3 4.1 4.0 4.0 6.6 6.7 6.5 7.9 7.8 7.8 6.9 7.4 7.5
Std Deviation (1e-4) 0.8 1.0 1.9 2.4 2.2 1.3 1.3 1.2 1.1 1.0 1.2 1.0 1.2 1.3 1.2 1.6 1.6 1.9 1.0 1.2 1.2
Intensity Change (Log2) -0.05 -0.23 -0.47 -0.48 -0.49 -0.44 -0.53 -0.54 -0.55 0.18 0.2 0.15 0.42 0.41 0.41 0.24 0.33 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 1.0 -1.4 -1.3 -1.0 -1.9 -2.9 -1.2 -3.0 3.8 5.4 5.6 3.9 2.5 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 6.7 0 0 0 0 0 0 0 -3.2 -3.2 -3.4 0.2 -0.4 0.6
Nucleus -1.6 -2.3 -1.7 -3.5 -3.0 -3.3 -3.6 -5.5 -4.3 -2.6 -4.7 -4.3 -3.6 -2.3 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.5 0
Nucleolus 0 -2.5 0 0 0 0 0 0 0 -3.5 -3.5 -3.8 -2.2 -2.3 -3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 -1.7 0 -0.9 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.343 3.5267 3.3592 3.2325 2.9421 2.9994 3.5425 3.1808 3.0545 3.4151 2.8206 2.7262 2.3575 2.6457 2.3536 2.2372 2.5945 2.5008
Actin 0.01 0.0001 0.0029 0.0013 0.0007 0.0001 0.0466 0 0.006 0.0631 0.0015 0.0002 0.0001 0 0 0 0 0
Bud 0.0003 0.002 0.0018 0.0001 0.0001 0.0001 0.0009 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0 0 0
Bud Neck 0.0011 0.0001 0.0015 0.0002 0.0007 0.0015 0.0092 0.0001 0.0036 0.0007 0.001 0.0009 0.0001 0.0001 0 0 0.0001 0.0003
Bud Periphery 0.0006 0.0006 0.001 0.0002 0.0002 0.0001 0.0018 0.0002 0.0003 0.0003 0.0004 0.0002 0.0002 0 0 0 0 0
Bud Site 0.003 0.0005 0.0091 0.0008 0.0005 0.0001 0.0044 0.0004 0.0002 0.0003 0.0008 0.0001 0.0001 0 0 0 0 0
Cell Periphery 0.0004 0 0.0004 0.0001 0.0001 0 0.0004 0 0.0001 0 0.0002 0.0001 0 0 0 0 0 0
Cytoplasm 0.0067 0.0022 0.0047 0.0013 0.0004 0.0002 0.0083 0.0003 0.0013 0.0006 0.0016 0.0003 0.0001 0.0004 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0057 0.0012 0.003 0.0015 0.0029 0.0005 0.011 0.0036 0.0004 0.0103 0.0008 0.0027 0 0.0002 0 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0027 0 0.0012 0.0035 0.0006 0 0.0066 0 0.0009 0.0008 0.0021 0.0001 0.0001 0 0 0 0 0
Endosome 0.009 0 0.0026 0.0095 0.017 0.0022 0.0171 0 0.0025 0.0044 0.012 0.0002 0.0006 0.0001 0 0 0 0
Golgi 0.0046 0 0.001 0.0011 0.0041 0.0018 0.0048 0 0.0004 0.0038 0.0006 0.0001 0 0 0 0 0 0
Lipid Particles 0.0042 0 0.0013 0.0025 0.0057 0.0005 0.004 0.0001 0.0004 0.0045 0.0024 0.0007 0.0001 0.0001 0 0 0 0
Mitochondria 0.012 0.0007 0.001 0.0005 0.0034 0.002 0.0045 0.0008 0.0079 0.0006 0.0032 0.0018 0.0013 0.0001 0.0001 0 0.0001 0
None 0.0045 0.0034 0.0026 0.0004 0.0002 0.0002 0.0064 0.0007 0.0011 0.0011 0.003 0.0002 0.0001 0.0072 0.0001 0 0 0
Nuclear Periphery 0.0173 0.001 0.0025 0.0073 0.0139 0.0004 0.0241 0.0005 0.006 0.0019 0.0602 0.0011 0.0023 0.0006 0.0009 0.0003 0.0023 0.0001
Nucleolus 0.0187 0.0068 0.0107 0.0056 0.0423 0.0363 0.0128 0.0119 0.0086 0.0067 0.0673 0.0273 0.0107 0.01 0.0039 0.0031 0.0333 0.0185
Nucleus 0.858 0.9801 0.935 0.9596 0.8657 0.9449 0.8136 0.9761 0.9549 0.8925 0.827 0.949 0.9803 0.968 0.9947 0.9964 0.9634 0.9779
Peroxisomes 0.0012 0.0001 0.0019 0.0003 0.0006 0.0001 0.0039 0.0001 0.0001 0.0031 0.0003 0.0002 0 0 0 0 0 0
Punctate Nuclear 0.0372 0.0011 0.0145 0.0028 0.0381 0.0084 0.0124 0.0043 0.0029 0.0047 0.0092 0.0141 0.0026 0.013 0.0003 0.0002 0.0004 0.0032
Vacuole 0.0016 0.0001 0.0009 0.0009 0.0015 0.0004 0.0044 0.0001 0.001 0.0001 0.0034 0.0003 0.0006 0.0001 0 0 0 0
Vacuole Periphery 0.0013 0 0.0002 0.0003 0.0012 0.0002 0.002 0 0.0012 0.0001 0.0028 0.0002 0.0005 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5206 10.5334 12.2826 10.3214 12.5492 15.1413 10.4601 13.3496 15.4108 11.0524
Translational Efficiency 1.5838 1.3087 1.111 1.4046 1.2798 1.1347 1.3346 0.9172 0.9611 1.3328

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2099 656 2073 1676 1579 922 1756 116 3678 1578 3829 1792

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.34 879.76 876.86 905.47 732.86 803.03 871.10 1015.56 713.73 834.93 874.22 912.60
Standard Deviation 95.26 124.37 118.22 124.20 93.79 107.20 120.50 181.51 96.08 120.73 119.30 131.51
Intensity Change Log 2 0.331116 0.326352 0.372673 0.131916 0.249301 0.470666 0.232622 0.287439 0.423647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000692 0.000599 0.001162 0.000087 0.000262 0.000655 0.001557 0.000121 0.000441 0.000625 0.001187
Bud Neck 0.004191 0.006705 0.025265 0.021636 0.005022 0.009943 0.011468 0.014524 0.004547 0.008597 0.018938 0.021176
Bud Site 0.000498 0.001274 0.002690 0.007841 0.000893 0.000858 0.001324 0.004843 0.000668 0.001031 0.002064 0.007647
Cell Periphery 0.000154 0.000101 0.000064 0.000136 0.000185 0.000059 0.000072 0.000196 0.000167 0.000076 0.000067 0.000140
Cytoplasm 0.007795 0.003671 0.001696 0.008891 0.012224 0.004217 0.003294 0.015545 0.009696 0.003990 0.002429 0.009322
Cytoplasmic Foci 0.001661 0.002127 0.000029 0.001206 0.001404 0.000424 0.000250 0.001366 0.001551 0.001132 0.000130 0.001216
Eisosomes 0.000017 0.000027 0.000025 0.000028 0.000012 0.000015 0.000026 0.000031 0.000015 0.000020 0.000025 0.000028
Endoplasmic Reticulum 0.002566 0.007107 0.004329 0.008386 0.003011 0.003555 0.004910 0.009539 0.002757 0.005032 0.004595 0.008461
Endosome 0.000542 0.001391 0.000212 0.002022 0.000368 0.000391 0.000625 0.005017 0.000467 0.000806 0.000401 0.002216
Golgi 0.000252 0.001463 0.000087 0.001910 0.000055 0.000260 0.000141 0.000503 0.000168 0.000760 0.000112 0.001819
Lipid Particles 0.000904 0.001148 0.000063 0.000210 0.000497 0.000328 0.000162 0.000958 0.000729 0.000669 0.000108 0.000258
Mitochondria 0.001028 0.002055 0.001534 0.005481 0.001296 0.003747 0.001745 0.001353 0.001143 0.003044 0.001630 0.005213
Mitotic Spindle 0.001073 0.005505 0.002744 0.019331 0.000352 0.005084 0.001818 0.002480 0.000764 0.005259 0.002319 0.018240
None 0.009285 0.003881 0.001827 0.004705 0.007346 0.002470 0.005116 0.013728 0.008453 0.003057 0.003335 0.005289
Nuclear Periphery 0.000727 0.001293 0.000714 0.002227 0.000892 0.001186 0.000633 0.000917 0.000798 0.001231 0.000677 0.002142
Nuclear Periphery Foci 0.000462 0.001248 0.000137 0.001358 0.000251 0.000451 0.000187 0.009468 0.000372 0.000782 0.000160 0.001883
Nucleolus 0.019832 0.014969 0.011695 0.007546 0.009011 0.012212 0.013756 0.003679 0.015186 0.013358 0.012640 0.007295
Nucleus 0.947800* 0.942419* 0.944693* 0.891727* 0.955702* 0.953133* 0.951408* 0.901579* 0.951192* 0.948679* 0.947772* 0.892365*
Peroxisomes 0.000475 0.001529 0.000134 0.000276 0.000159 0.000486 0.000350 0.000237 0.000339 0.000919 0.000233 0.000273
Vacuole 0.000432 0.000963 0.001163 0.009984 0.000889 0.000603 0.001821 0.012203 0.000629 0.000753 0.001464 0.010127
Vacuole Periphery 0.000158 0.000431 0.000300 0.003937 0.000343 0.000317 0.000240 0.000275 0.000237 0.000364 0.000273 0.003700

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.92 -18.78 -14.11 -5.87 -6.68 -7.73 -17.22 -6.11 -5.50 -3.88 -10.83 -25.23 -15.60 -10.74 -7.41
Bud Neck -2.84 -19.47 -16.28 -11.14 2.42 -3.42 -9.49 -2.80 -1.61 -0.93 -4.47 -21.35 -16.50 -10.22 -1.76
Bud Site -2.26 -4.78 -6.66 -5.12 -3.31 0.02 -1.75 -2.29 -2.33 -1.90 -1.82 -5.05 -6.67 -6.08 -4.46
Cell Periphery 1.87 4.93 1.90 -0.55 -5.24 3.45 3.10 0.18 -2.52 -2.23 4.34 5.32 2.29 -3.24 -5.40
Cytoplasm 2.94 6.94 -0.34 -3.35 -8.08 4.39 5.93 -0.83 -2.14 -2.37 5.08 8.82 0.81 -4.15 -7.42
Cytoplasmic Foci -0.26 4.32 2.74 1.87 -3.18 1.85 2.26 0.91 -0.59 -0.96 1.23 4.62 2.86 1.32 -2.79
Eisosomes -4.96 -7.86 -11.58 -3.68 -5.71 -2.70 -13.05 -6.57 -5.90 -3.71 -4.55 -14.17 -15.82 -10.41 -6.43
Endoplasmic Reticulum -9.72 -10.69 -16.12 -3.43 -9.87 -1.78 -7.36 -4.88 -4.56 -3.55 -8.70 -12.63 -16.41 -9.59 -10.28
Endosome -1.18 1.25 -2.49 0.09 -5.14 -0.25 -1.22 -1.55 -1.53 -1.41 -1.06 0.41 -3.70 -1.73 -4.36
Golgi -1.55 1.11 -1.65 0.57 -2.19 -1.29 -1.78 -2.19 -0.59 -1.57 -1.72 0.67 -1.99 -0.37 -2.19
Lipid Particles -0.27 2.86 2.24 1.64 -7.81 0.72 1.16 -0.61 -1.52 -2.00 0.43 2.94 2.14 1.52 -5.38
Mitochondria -2.25 -3.76 -5.82 -3.21 -4.30 -1.78 -1.08 -1.61 1.19 -0.74 -2.32 -2.74 -5.53 -1.14 -4.36
Mitotic Spindle -2.11 -2.79 -6.60 -3.43 -4.82 -3.02 -2.82 -1.95 0.60 -1.06 -3.51 -3.56 -6.96 -4.04 -5.62
None 3.29 6.96 4.49 -0.19 -4.77 4.29 2.32 -1.05 -2.88 -2.13 5.65 6.51 4.52 -1.95 -2.47
Nuclear Periphery -1.24 -2.06 -8.62 -2.84 -7.43 -1.44 0.66 -2.94 -1.32 -3.27 -1.97 -1.02 -8.62 -4.52 -8.13
Nuclear Periphery Foci -1.38 1.89 -2.37 0.17 -4.56 -0.92 0.66 -1.13 -1.09 -1.15 -1.45 1.99 -2.82 -1.56 -3.55
Nucleolus 2.76 3.86 8.57 4.86 5.42 -2.19 -4.09 2.85 4.07 5.84 1.44 1.32 7.58 5.05 6.92
Nucleus 0.93 3.18 9.18 5.98 7.15 0.56 1.89 3.93 3.68 3.52 0.69 3.53 11.00 8.95 9.24
Peroxisomes -1.81 2.82 2.15 2.55 -2.42 -1.93 -2.00 -1.96 1.03 0.28 -2.07 1.83 1.59 2.83 -0.46
Vacuole -3.44 -5.51 -8.89 -8.36 -7.73 1.49 -2.87 -2.98 -3.02 -2.74 -1.22 -4.78 -9.16 -9.03 -7.63
Vacuole Periphery -1.93 -1.03 -3.84 -3.17 -3.47 0.21 1.07 0.61 0.36 -0.49 -1.51 -0.33 -3.68 -3.37 -3.59