Standard name
Human Ortholog
Description Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.06 0.08 0.05 0.09 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.98 0.96 0.9 0.8 0.71 0.71 0.71 0.63 0.99 1.0 0.99 1.0 0.93 0.93 0.97 0.94 0.96 0.92 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.1 0.28 0.2 0.35 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.1 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 10 7 7 6 10 0 0 0 0 0 0 0 1 0 0 0 1
Bud 1 0 4 3 5 1 7 6 12 8 15 2 0 1 0 0 0 1 1 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cell Periphery 6 10 1 5 10 11 25 33 39 21 50 3 7 6 3 0 2 0 0 0 0 0 0
Cytoplasm 393 331 128 232 407 451 399 400 351 304 342 242 380 420 40 25 26 394 320 124 82 274 307
Endoplasmic Reticulum 1 7 1 3 10 19 17 3 18 7 20 1 0 4 1 1 1 0 1 0 0 2 2
Endosome 0 0 0 0 1 0 0 3 7 2 3 0 0 0 0 0 0 0 5 1 3 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 4 2
Mitochondria 2 0 3 6 4 28 50 158 101 150 209 1 0 1 0 0 0 0 1 0 0 0 0
Nucleus 14 3 2 0 2 2 9 6 13 6 7 2 0 1 1 0 1 0 0 1 0 1 1
Nuclear Periphery 0 1 0 1 0 0 2 3 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 1 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 11 16 35 55 25 15 13 0 1 2 0 2 1 5 3 0 0 4 6
Unique Cell Count 404 339 130 236 426 500 498 561 496 427 542 245 381 426 40 27 28 408 342 130 90 297 333
Labelled Cell Count 418 353 139 250 450 528 556 678 576 520 676 251 388 436 45 28 32 408 342 130 90 297 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 11.4 6.0 7.4 8.1 7.0 6.7 6.0 6.7 5.9 5.6 10.0 10.8 10.3 13.3 13.8 14.0 8.6 10.2 10.1
Std Deviation (1e-4) 1.4 1.6 1.0 1.0 1.5 1.2 1.3 1.2 7.4 1.3 1.2 1.6 1.6 1.4 4.0 2.3 3.5 1.2 1.6 1.8
Intensity Change (Log2) 0.3 0.43 0.22 0.16 -0.0 0.15 -0.02 -0.1 0.74 0.85 0.77 1.14 1.2 1.22 0.52 0.76 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.2 2.4 2.9 2.1 3.3 0 0 0 0 0 0
Cytoplasm -0.1 -1.5 -3.1 -5.1 -6.6 -6.6 -6.5 -7.9 0.3 1.6 0.1 0.8 -1.8 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.5 2.8 6.3 4.9 7.3 8.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 3.7 2.6 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1086 5.6592 4.6725 4.9164 4.4868 5.5769 4.8423 5.8067 4.9127 3.9713 4.5696 5.1445 6.5334 8.0543 7.3297 6.4975 6.5231 6.8554
Actin 0.0549 0.0009 0.0765 0.1975 0.018 0.0009 0.0085 0.0043 0.0291 0.0153 0.0013 0.0087 0.0285 0.001 0.0124 0.0009 0.0303 0.0013
Bud 0.0029 0.0001 0.002 0.0008 0.0005 0 0.0004 0.0002 0.0011 0.0013 0.0004 0.0002 0.0006 0.0001 0.0008 0.0006 0.0002 0
Bud Neck 0.0053 0.0004 0.0039 0.0038 0.0038 0.0013 0.0014 0.0004 0.0024 0.0043 0.0026 0.0031 0.0005 0.0002 0.0015 0.0009 0.0011 0.0026
Bud Periphery 0.0062 0.0001 0.0019 0.0007 0.0004 0 0.0005 0.0001 0.0011 0.0015 0.0006 0.0002 0.0009 0.0001 0.0007 0.0003 0.0003 0
Bud Site 0.0133 0.0011 0.0124 0.0022 0.0093 0 0.003 0.0012 0.0073 0.0015 0.0029 0.0007 0.0009 0.0006 0.002 0.001 0.0008 0.0001
Cell Periphery 0.0006 0.0002 0.0005 0.0002 0.0002 0.0001 0.0003 0.0002 0.0003 0.0003 0.0004 0.0001 0.0002 0.0002 0.0001 0 0.0001 0
Cytoplasm 0.6531 0.9652 0.7086 0.7425 0.7351 0.9268 0.8368 0.9776 0.8574 0.8111 0.9098 0.9007 0.8436 0.9799 0.9133 0.93 0.7824 0.9677
Cytoplasmic Foci 0.0452 0.0015 0.0491 0.0053 0.0424 0.0024 0.0177 0.0025 0.0224 0.0189 0.0045 0.0167 0.0065 0.0018 0.0227 0.0079 0.0247 0.009
Eisosomes 0.0004 0 0.0005 0.0003 0.0002 0 0.0001 0 0.0002 0 0 0 0.0004 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0314 0.001 0.0021 0.0022 0.0038 0.0025 0.006 0.0022 0.0039 0.0146 0.0026 0.0041 0.0056 0.0013 0.0013 0.0029 0.0005 0.0016
Endosome 0.042 0.001 0.0486 0.0069 0.0038 0.0108 0.0224 0.0007 0.017 0.0847 0.0286 0.0278 0.0088 0.0004 0.0071 0.0174 0.0145 0.0018
Golgi 0.0149 0.0018 0.0233 0.0251 0.0065 0.03 0.0135 0.0005 0.0162 0.0244 0.012 0.0111 0.0094 0.0001 0.0095 0.0014 0.0242 0.0022
Lipid Particles 0.0139 0.0009 0.009 0.0022 0.0206 0.0001 0.0168 0.0001 0.0029 0.0017 0.0019 0.0029 0.0173 0 0.0098 0.0002 0.0323 0.0015
Mitochondria 0.0058 0.0136 0.0178 0.0027 0.0126 0.0152 0.0097 0.0001 0.0132 0.0044 0.0131 0.0028 0.011 0 0.0068 0.0016 0.0222 0.0002
None 0.0735 0.0064 0.0219 0.0029 0.0013 0.008 0.0361 0.0078 0.0088 0.0013 0.0052 0.0028 0.0309 0.0123 0.0029 0.0248 0.0008 0.0012
Nuclear Periphery 0.0091 0.0004 0.0009 0.0008 0.0005 0.0002 0.0087 0.0003 0.0033 0.004 0.0006 0.0049 0.0174 0.0003 0.0004 0.0022 0.0002 0.0003
Nucleolus 0.0011 0 0.0005 0 0.0003 0 0.0027 0 0.0002 0.0001 0.0004 0.0002 0.0002 0 0 0.0001 0.0001 0
Nucleus 0.0032 0.0028 0.0024 0.0012 0.0008 0.0008 0.0058 0.0014 0.0027 0.0024 0.0016 0.0077 0.0064 0.0011 0.0015 0.0038 0.0008 0.0017
Peroxisomes 0.0079 0.0022 0.0125 0.0017 0.1389 0.0001 0.0019 0.0001 0.0038 0.0012 0.0005 0.0005 0.007 0 0.0042 0.0008 0.0617 0.0083
Punctate Nuclear 0.0099 0.0002 0.0022 0.0005 0.0006 0.0001 0.0037 0.0003 0.0045 0.0005 0.0002 0.0023 0.0019 0.0003 0.0024 0.0007 0.0018 0.0003
Vacuole 0.004 0.0003 0.0028 0.0002 0.0004 0.0003 0.0026 0.0001 0.0014 0.0038 0.0088 0.0019 0.0007 0.0002 0.0003 0.002 0.0004 0.0002
Vacuole Periphery 0.0016 0.0001 0.0007 0.0002 0.0001 0.0003 0.0015 0 0.0006 0.0028 0.002 0.0007 0.0013 0 0.0001 0.0006 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5086 25.0508 16.2652 22.8159 22.7081 24.5065 25.3289 35.259 25.6553 31.8453
Translational Efficiency 4.0136 1.5805 1.7141 1.843 1.968 2.1681 1.9153 1.3492 2.7539 1.6734

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency