Standard name
Description Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0.05 0.06 0.08 0.05 0.09 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.98 0.96 0.9 – 0.8 0.71 0.71 0.71 0.63 0.99 1.0 0.99 1.0 0.93 0.93 0.97 0.94 0.96 0.92 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 – 0.1 0.28 0.2 0.35 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.07 0.1 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 10 7 7 6 10 0 0 0 0 0 0 0 1 0 0 0 1
Bud 1 0 4 3 5 1 – 7 6 12 8 15 2 0 1 0 0 0 1 1 0 0 1 3
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2 1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 10 1 5 10 11 – 25 33 39 21 50 3 7 6 3 0 2 0 0 0 0 0 0
Cytoplasm 393 331 128 232 407 451 – 399 400 351 304 342 242 380 420 40 25 26 394 320 124 82 274 307
Endoplasmic Reticulum 1 7 1 3 10 19 – 17 3 18 7 20 1 0 4 1 1 1 0 1 0 0 2 2
Endosome 0 0 0 0 1 0 – 0 3 7 2 3 0 0 0 0 0 0 0 5 1 3 2 1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 4 2
Mitochondria 2 0 3 6 4 28 – 50 158 101 150 209 1 0 1 0 0 0 0 1 0 0 0 0
Nucleus 14 3 2 0 2 2 – 9 6 13 6 7 2 0 1 1 0 1 0 0 1 0 1 1
Nuclear Periphery 0 1 0 1 0 0 – 2 3 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 – 1 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 11 16 – 35 55 25 15 13 0 1 2 0 2 1 5 3 0 0 4 6
Unique Cell Count 404 339 130 236 426 500 498 561 496 427 542 245 381 426 40 27 28 408 342 130 90 297 333
Labelled Cell Count 418 353 139 250 450 528 556 678 576 520 676 251 388 436 45 28 32 408 342 130 90 297 333


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 11.4 6.0 7.4 8.1 7.0 – 6.7 6.0 6.7 5.9 5.6 10.0 10.8 10.3 13.3 13.8 14.0 8.6 10.2 10.1
Std Deviation (1e-4) 1.4 1.6 1.0 1.0 1.5 1.2 – 1.3 1.2 7.4 1.3 1.2 1.6 1.6 1.4 4.0 2.3 3.5 1.2 1.6 1.8
Intensity Change (Log2) – – – 0.3 0.43 0.22 – 0.16 -0.0 0.15 -0.02 -0.1 0.74 0.85 0.77 1.14 1.2 1.22 0.52 0.76 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 2.2 2.4 2.9 2.1 3.3 0 0 0 0 0 0
Cytoplasm -0.1 -1.5 -3.1 – -5.1 -6.6 -6.6 -6.5 -7.9 0.3 1.6 0.1 0.8 -1.8 -1.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.5 – 2.8 6.3 4.9 7.3 8.0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 3.1 3.7 2.6 0 0 0 0 0 0 0 0