Standard name | |
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Human Ortholog | |
Description | Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 4 | 2 | 2 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 |
Bud Site | 0 | 1 | 0 | 1 | 0 | 13 | 3 | 13 | 6 | 12 | 15 | 20 | 0 | 1 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 8 | 3 | 3 | 7 | 15 | 21 | 35 | 9 | 16 | 8 | 7 | 17 | 27 | 46 | 36 | 24 | 16 | 0 | 2 | 0 | 1 | 1 | 2 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 12 | 8 | 5 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 2 | 1 | 2 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 5 | 1 | 1 | 5 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 1 |
Mitochondria | 8 | 42 | 3 | 4 | 4 | 110 | 20 | 44 | 77 | 67 | 71 | 81 | 8 | 1 | 2 | 13 | 11 | 13 | 2 | 8 | 0 | 1 | 3 | 7 |
Nucleus | 287 | 517 | 227 | 222 | 337 | 336 | 379 | 511 | 317 | 365 | 344 | 445 | 187 | 225 | 261 | 265 | 191 | 191 | 240 | 490 | 197 | 184 | 204 | 157 |
Nuclear Periphery | 1 | 3 | 4 | 1 | 1 | 2 | 8 | 5 | 7 | 2 | 4 | 4 | 0 | 0 | 2 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 127 | 67 | 18 | 8 | 16 | 78 | 57 | 135 | 114 | 118 | 141 | 214 | 30 | 33 | 33 | 1 | 2 | 1 | 96 | 62 | 37 | 50 | 72 | 67 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 3 | 7 | 1 | 5 | 5 | 13 | 9 | 2 | 7 | 9 | 9 | 2 | 4 | 6 | 3 | 2 | 1 | 3 | 8 | 3 | 3 | 11 | 7 |
Vac/Vac Membrane | 2 | 3 | 2 | 0 | 2 | 0 | 5 | 6 | 3 | 2 | 2 | 4 | 0 | 0 | 1 | 13 | 14 | 6 | 8 | 18 | 9 | 9 | 16 | 15 |
Unique Cell Count | 346 | 570 | 241 | 230 | 355 | 409 | 455 | 631 | 410 | 486 | 476 | 608 | 218 | 264 | 321 | 320 | 232 | 217 | 360 | 611 | 257 | 260 | 330 | 273 |
Labelled Cell Count | 431 | 646 | 266 | 242 | 373 | 562 | 511 | 766 | 540 | 593 | 603 | 797 | 245 | 293 | 356 | 348 | 255 | 236 | 360 | 611 | 257 | 260 | 330 | 273 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 6.5 | 7.2 | 6.9 | 7.2 | 4.9 | 6.4 | 6.2 | 5.6 | 5.7 | 5.7 | 5.6 | 6.7 | 7.4 | 7.0 | 7.2 | 7.1 | 6.8 | 6.1 | 6.4 | 7.0 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.3 | 1.6 | 1.5 | 1.3 | 1.5 | 1.4 | 1.5 | 1.2 | 1.3 | 1.2 | 1.7 | 1.9 | 1.9 | 1.8 | 1.7 | 1.6 | 1.7 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.02 | -0.54 | -0.17 | -0.2 | -0.34 | -0.34 | -0.33 | -0.35 | -0.09 | 0.05 | -0.03 | 0.01 | -0.02 | -0.07 | -0.23 | -0.17 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.8 | 2.3 | 2.8 | 0 | 1.6 | 0 | 0 | 3.4 | 4.3 | 5.4 | 4.6 | 4.3 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 8.3 | 2.2 | 3.3 | 6.6 | 5.4 | 5.7 | 5.3 | 1.7 | 0 | 0 | 2.0 | 2.2 | 2.8 |
Nucleus | 1.2 | 0.4 | -4.4 | -4.1 | -4.8 | -5.6 | -6.2 | -6.9 | -6.8 | -3.0 | -3.3 | -4.5 | -4.1 | -4.0 | -2.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.9 | -1.5 | 4.0 | 2.0 | 4.8 | 6.2 | 5.5 | 6.7 | 8.2 | 2.2 | 1.9 | 1.1 | -4.6 | -3.6 | -3.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0 | 0 | -1.1 | -0.8 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 3.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3448 | 2.2558 | 2.2216 | 2.089 | 2.0852 | 2.1165 | 4.2794 | 3.2015 | 3.5568 | 3.3154 | 3.5252 | 3.6379 | 6.403 | 6.4944 | 5.9993 | 6.0663 | 5.2842 | 5.8693 |
Actin | 0.0151 | 0 | 0.0226 | 0.005 | 0.0318 | 0.0025 | 0.0048 | 0 | 0 | 0 | 0.0001 | 0 | 0.0094 | 0 | 0.0001 | 0 | 0.0006 | 0 |
Bud | 0.0005 | 0 | 0.0002 | 0.0005 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Bud Neck | 0.0048 | 0 | 0.0015 | 0.0022 | 0.0008 | 0.0005 | 0.0008 | 0.0001 | 0 | 0 | 0.0004 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0015 | 0.0034 |
Bud Periphery | 0.001 | 0 | 0.0004 | 0.0009 | 0.0008 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.0009 | 0.0001 |
Bud Site | 0.0132 | 0 | 0.0024 | 0.0028 | 0.0018 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.001 | 0.0002 |
Cell Periphery | 0.0009 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 |
Cytoplasm | 0.004 | 0.0001 | 0.0008 | 0.0015 | 0.0152 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.007 | 0 | 0.0012 | 0.028 | 0.0306 | 0.0011 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Eisosomes | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0021 | 0 | 0.0017 | 0.0017 | 0.0021 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Endosome | 0.0181 | 0 | 0.0035 | 0.0295 | 0.0218 | 0.0024 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0011 | 0 | 0.0007 | 0 | 0.0007 | 0 |
Golgi | 0.0083 | 0 | 0.002 | 0.0102 | 0.0129 | 0.001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0018 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Lipid Particles | 0.0142 | 0 | 0.0013 | 0.0627 | 0.0466 | 0.005 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0076 | 0 | 0.0022 | 0 | 0.0011 | 0 |
Mitochondria | 0.0046 | 0.0001 | 0.0011 | 0.0071 | 0.0147 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0114 | 0.0001 | 0.0079 | 0.0001 |
None | 0.0049 | 0 | 0.0007 | 0.0013 | 0.0143 | 0.0003 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0005 | 0 |
Nuclear Periphery | 0.0225 | 0.0006 | 0.0063 | 0.009 | 0.013 | 0.0016 | 0.003 | 0.0004 | 0.0028 | 0.0001 | 0.0048 | 0.0009 | 0.0012 | 0.0002 | 0.0009 | 0.0001 | 0.0126 | 0.0001 |
Nucleolus | 0.0509 | 0.0323 | 0.0184 | 0.0425 | 0.0705 | 0.0751 | 0.0363 | 0.0274 | 0.0234 | 0.0222 | 0.1984 | 0.0856 | 0.0391 | 0.0642 | 0.0372 | 0.0199 | 0.2108 | 0.09 |
Nucleus | 0.8094 | 0.965 | 0.9285 | 0.7431 | 0.6702 | 0.9039 | 0.9433 | 0.9697 | 0.9732 | 0.9697 | 0.791 | 0.9116 | 0.9241 | 0.9003 | 0.9413 | 0.9796 | 0.7091 | 0.9002 |
Peroxisomes | 0.0023 | 0 | 0.0004 | 0.0187 | 0.0127 | 0.0024 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0083 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.013 | 0.0016 | 0.0057 | 0.0299 | 0.033 | 0.0033 | 0.0052 | 0.0023 | 0.0002 | 0.0078 | 0.0042 | 0.0005 | 0.002 | 0.035 | 0.0011 | 0.0002 | 0.0493 | 0.0042 |
Vacuole | 0.0015 | 0 | 0.0005 | 0.0021 | 0.0039 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.001 | 0 | 0.002 | 0 | 0.001 | 0.0012 |
Vacuole Periphery | 0.0018 | 0 | 0.0003 | 0.0011 | 0.0021 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0012 | 0 | 0.0014 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.8496 | 24.4153 | 20.5328 | 16.6036 | 25.9682 | 16.3781 | 25.0977 | 27.2139 | 22.0329 | 18.6829 |
Translational Efficiency | 1.9102 | 2.0703 | 1.5574 | 1.6486 | 1.441 | 2.4426 | 1.6841 | 1.4415 | 1.5432 | 1.9993 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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