Standard name
Description Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.05 0.06 0.07 0.08 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.19 0.05 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0 0.07 0.11 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.11 0.09 0.05 0.14 0.08 0.18 0.18 0.22 0.21 0 0 0 0 0.14 0.07 0 0 0 0 0 0
Nucleus 0.17 0.28 0.16 0.08 0.07 0.07 0.06 0.05 0.06 0.07 0.07 0.11 0.07 0.05 0.09 0.19 0.09 0.16 0.16 0.06 0.05 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.86 0.83 0.93 0.86 0.8 0.79 0.78 0.75 0.72 0.75 0.75 0.83 0.79 0.76 0.88 0.75 0.82 0.69 0.7 0.59 0.61 0.57 0.56
Peroxisomes 0 0 0 0.05 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.15 0.15 0.25 0.37 0.46 0.45 0.5 0.48 0.5 0.44 0.51 0.24 0.31 0.33 0.18 0.09 0.23 0.11 0.07 0.26 0.24 0.22 0.28
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 2 5 3 8 12 11 21 20 7 0 0 0 0 1 1 0 2 3 3 4 7
Bud Neck 5 0 21 14 18 10 10 13 26 11 13 9 9 4 1 0 2 0 3 4 0 4 1
Bud Site 1 0 7 6 8 18 19 29 45 44 56 1 1 0 1 3 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 0 2 8 17 21 20 5 17 4 4 26 46 63 6 14 10 0 0 0 3 2 3
Endoplasmic Reticulum 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 2 2 0 0 0 1 3 0
Endosome 1 0 1 4 43 30 47 14 25 19 15 10 25 36 0 3 7 1 2 10 4 10 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 9 4 49 32 25 88 65 103 141 133 153 3 3 3 3 22 12 1 0 1 0 1 1
Nucleus 29 58 69 26 34 44 48 30 51 44 50 31 27 18 11 30 16 26 36 28 9 19 8
Nuclear Periphery 0 2 2 1 1 2 5 3 1 4 1 0 0 0 0 0 1 0 0 0 0 1 2
Nucleolus 144 172 405 297 366 494 607 420 575 462 546 225 286 257 109 119 141 115 160 258 119 179 177
Peroxisomes 0 2 3 19 18 27 49 30 23 21 28 2 4 2 1 0 2 0 0 1 0 1 1
SpindlePole 25 32 109 128 211 283 391 270 398 272 370 65 112 110 22 14 39 17 16 115 47 68 89
Vac/Vac Membrane 0 1 0 2 3 2 3 1 0 0 1 2 0 0 0 2 2 1 2 5 3 5 4
Unique Cell Count 168 207 434 346 458 629 779 558 803 614 727 270 360 336 124 159 173 168 229 435 198 315 319
Labelled Cell Count 218 271 671 543 747 1028 1277 929 1323 1034 1244 374 513 493 154 210 235 168 229 435 198 315 319


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 10.6 6.3 7.2 6.7 5.4 5.7 4.9 5.0 5.0 5.0 8.2 8.4 8.1 13.1 11.4 12.4 11.0 10.1 9.9
Std Deviation (1e-4) 1.8 2.4 1.7 2.6 1.8 1.7 1.9 1.6 1.5 1.4 1.5 2.3 2.3 2.6 4.2 4.0 4.0 5.7 2.6 2.5
Intensity Change (Log2) 0.19 0.1 -0.21 -0.14 -0.37 -0.33 -0.34 -0.33 0.38 0.41 0.37 1.06 0.86 0.98 0.8 0.68 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.7 0 2.7 3.1 0 0 0 0 0 0 0
Bud Neck -0.5 -0.7 -3.1 -3.8 0 -1.4 -2.8 -3.0 -1.0 -1.7 -2.8 0 -2.8 -2.1
Bud Site 0.1 0.2 1.3 1.0 0 3.3 4.1 4.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.4 3.2 2.6 0 2.3 0 0 6.1 7.2 9.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 6.3 4.3 5.0 0 3.4 3.3 2.6 0 5.3 6.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.9 -3.2 1.3 -1.7 0 2.9 4.4 4.2 -5.0 -5.9 -5.7 -3.0 0.8 -1.6
Nucleus -3.6 -4.0 -4.6 -5.5 -5.5 -5.4 -4.5 -4.9 -1.6 -3.6 -4.6 -2.0 0.9 -2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -5.8 -6.6 -6.9 0 -9.0 -7.6 -7.8 -4.2 -5.8 -6.7 -2.0 -6.2 -4.4
Peroxisomes 4.0 3.2 3.5 4.6 0 2.5 2.9 3.2 0 0 0 0 0 0
SpindlePole 3.6 6.5 6.6 8.5 0 8.3 6.4 8.6 -0.3 1.9 2.3 -1.7 -4.3 -0.7
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available