Standard name
Human Ortholog
Description Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.08 0 0.08 0 0 0
Bud 0.06 0.08 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 – 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0.05 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.13 0 0.11 0.13 0.06 0.06 0.06 0 0.05 0.06 0.12 0.08 0.12 0.12 0.13 – 0 0.07 0 0 0.07 0.05 0 0
Cytoplasm 0.88 0.87 0.94 0.97 0.92 0.9 0.81 0.83 0.79 0.73 0.7 0.71 0.99 0.99 0.98 – 0.96 0.96 0.76 0.81 0.66 0.7 0.74 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.18 0.25 0.23 0.33 0.37 0.46 0.46 0 0 0 – 0 0 0 0 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 2 13 4 16 1 8 4 1 8 3 2 0 0 0 – 7 3 16 1 28 3 8 11
Bud 11 4 14 8 21 11 25 28 30 37 25 31 0 0 1 – 5 3 10 3 6 2 4 9
Bud Neck 2 0 2 0 5 3 3 3 0 0 0 0 1 1 0 – 7 0 8 1 16 5 9 11
Bud Site 1 1 0 0 0 0 0 5 0 2 2 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 26 0 37 41 33 23 43 39 28 43 62 51 14 14 22 – 18 17 8 0 25 4 7 8
Cytoplasm 173 46 314 309 501 360 569 726 449 492 366 426 115 115 167 – 411 223 164 59 229 76 150 231
Endoplasmic Reticulum 4 2 1 0 1 4 8 4 5 10 6 3 1 1 2 – 27 15 3 0 14 2 3 6
Endosome 0 0 2 1 6 2 6 1 1 1 1 5 0 0 0 – 4 1 0 2 3 0 2 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 2
Mitochondria 0 1 4 7 30 73 173 200 185 247 240 275 0 0 0 – 14 8 1 1 11 8 8 12
Nucleus 2 1 2 1 1 2 1 3 4 2 1 1 0 0 1 – 1 1 0 0 0 0 0 1
Nuclear Periphery 0 0 1 0 0 0 0 5 4 4 0 1 0 0 0 – 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 2 0 0 2 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 0
SpindlePole 3 0 1 0 8 0 3 8 3 5 0 6 0 0 0 – 2 2 0 0 0 0 0 3
Vac/Vac Membrane 0 0 0 1 7 1 3 8 4 3 1 3 0 0 0 – 1 1 1 0 1 1 4 7
Unique Cell Count 197 53 335 317 547 401 704 879 568 676 526 602 116 116 171 426 233 215 74 350 109 203 314
Labelled Cell Count 240 57 392 372 631 480 843 1038 714 855 707 806 131 131 193 497 274 215 74 350 109 203 314


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.5 5.0 5.1 5.0 4.3 4.0 4.4 4.1 3.9 4.0 4.0 6.1 6.1 6.2 4.7 5.2 6.0 6.9 6.7 7.0
Std Deviation (1e-4) 0.6 0.4 1.2 1.5 1.7 1.4 1.1 1.2 1.3 1.0 1.1 1.0 0.6 0.6 1.0 1.2 0.9 1.1 1.9 1.4 1.4
Intensity Change (Log2) – – – 0.01 0.0 -0.22 -0.34 -0.2 -0.28 -0.35 -0.35 -0.35 0.28 0.28 0.3 -0.09 0.06 0.26 0.46 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3703 2.1319 1.8382 1.1147 1.3627 1.7532 0.0049 0.9384 0.6173 0.2645 0.2688 0.4976 -0.1013 0.2374 -0.2141 0.5783 0.1548 -0.1439
Actin 0.0277 0.0067 0.0216 0.001 0.0024 0.0126 0.0172 0.0026 0.0579 0.0247 0.0099 0.0055 0.0525 0.0019 0.0048 0.0766 0.0046 0.0059
Bud 0.0012 0.0016 0.01 0.004 0.0002 0.0024 0.0099 0.0043 0.026 0.0155 0.0245 0.0024 0.0007 0.0003 0.0117 0.0072 0.0059 0.0005
Bud Neck 0.0022 0.0015 0.0075 0.0007 0.0007 0.0487 0.0114 0.0013 0.0019 0.0119 0.0478 0.0115 0.0012 0.0007 0.0004 0.0076 0.002 0.0365
Bud Periphery 0.0032 0.0032 0.0574 0.0099 0.0005 0.0061 0.0226 0.0138 0.0884 0.1076 0.0347 0.0053 0.0015 0.0006 0.0828 0.0663 0.0142 0.0015
Bud Site 0.0276 0.0326 0.0186 0.0025 0.0003 0.0031 0.0329 0.076 0.0274 0.0204 0.007 0.0006 0.0068 0.0058 0.0014 0.0029 0.0117 0.0032
Cell Periphery 0.0119 0.0068 0.0087 0.004 0.0028 0.0058 0.015 0.0057 0.0099 0.0067 0.0035 0.004 0.0071 0.0088 0.0096 0.0269 0.0016 0.0024
Cytoplasm 0.6812 0.5786 0.5859 0.7903 0.8095 0.6541 0.6123 0.5797 0.5213 0.5272 0.7759 0.7222 0.3929 0.6458 0.6486 0.549 0.4096 0.7085
Cytoplasmic Foci 0.0169 0.0071 0.011 0.0017 0.0189 0.019 0.0197 0.0055 0.0096 0.0109 0.0071 0.0023 0.0382 0.0024 0.0038 0.0102 0.0125 0.0094
Eisosomes 0.0007 0.0009 0.0007 0 0.0001 0.0003 0.0008 0.0003 0.0007 0.0002 0.0002 0.0002 0.0019 0.0015 0.0002 0.0007 0.0001 0.0006
Endoplasmic Reticulum 0.0077 0.0034 0.0038 0.002 0.0039 0.0026 0.0048 0.0014 0.0037 0.0022 0.0024 0.0015 0.0069 0.0037 0.0113 0.007 0.0018 0.0018
Endosome 0.0041 0.0021 0.0017 0.0011 0.0024 0.004 0.0125 0.0007 0.0049 0.0007 0.0007 0.0003 0.0084 0.0003 0.0013 0.0296 0.1028 0.0064
Golgi 0.0024 0.0007 0.0015 0.0002 0.0011 0.0016 0.0019 0.0004 0.0036 0.0006 0.0004 0.0002 0.0081 0.0003 0.0006 0.0283 0.0742 0.0065
Lipid Particles 0.0025 0.0004 0.0024 0.0001 0.0098 0.0039 0.0019 0.0003 0.0024 0.0002 0.0002 0.0001 0.0146 0.0005 0.0011 0.0155 0.0067 0.0023
Mitochondria 0.0003 0.0002 0.0016 0.0002 0.0003 0.0008 0.0025 0.0003 0.0124 0.0006 0.0003 0.0001 0.0419 0.0001 0.0002 0.0384 0.1477 0.0088
None 0.2044 0.3478 0.2531 0.1747 0.1235 0.2262 0.1973 0.3039 0.2133 0.2677 0.0815 0.2428 0.4026 0.3257 0.2177 0.1164 0.0908 0.1919
Nuclear Periphery 0.0005 0.0003 0.0006 0.0003 0.0003 0.0017 0.0028 0.0004 0.0009 0.0004 0.0003 0.0001 0.0008 0.0002 0.0008 0.0009 0.0016 0.0002
Nucleolus 0.0002 0.0003 0.0015 0.0001 0.0001 0.0002 0.0027 0.0003 0.0004 0.0001 0.0002 0 0.0004 0.0002 0.0001 0.0005 0.0021 0.0002
Nucleus 0.0006 0.0012 0.0015 0.0007 0.0003 0.0011 0.0056 0.0009 0.0011 0.0006 0.001 0.0002 0.0005 0.0003 0.0005 0.0006 0.0038 0.0004
Peroxisomes 0.0014 0.0002 0.0076 0 0.0191 0.0028 0.0176 0.0002 0.0096 0.0003 0.0002 0 0.0084 0 0 0.0074 0.0036 0.0114
Punctate Nuclear 0.0013 0.0008 0.0012 0.0001 0.0023 0.0019 0.003 0.0008 0.0023 0.0003 0.0005 0.0001 0.0012 0.0002 0.0003 0.0004 0.0003 0.0002
Vacuole 0.002 0.0034 0.0019 0.006 0.0014 0.001 0.0048 0.0011 0.0021 0.0011 0.0017 0.0005 0.0025 0.0007 0.0026 0.0057 0.084 0.001
Vacuole Periphery 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0007 0.0001 0.0003 0.0001 0.0001 0 0.0007 0 0.0001 0.0019 0.0183 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.239 6.8199 8.2569 12.5537 14.5768 17.2562 13.7005 11.3902 13.937 16.0319
Translational Efficiency 1.6228 1.4591 1.075 0.9789 1.0525 1.0648 0.8354 0.9918 0.8111 0.8872

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
778 433 299 116 1916 1358 776 1760 2694 1791 1075 1876

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 804.97 726.17 1005.23 957.75 642.98 742.77 822.80 845.60 689.76 738.76 873.54 852.53
Standard Deviation 99.30 92.56 111.50 112.80 102.29 89.87 87.79 114.52 125.22 90.81 125.31 117.56
Intensity Change Log 2 — -0.148628 0.320519 0.250714 — 0.208142 0.355768 0.395202 — 0.020764 0.336278 0.316668

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003969 0.001080 0.001274 0.000716 0.001044 0.001782 0.001088 0.003888 0.001889 0.001612 0.001139 0.003692
Bud Neck 0.014489 0.048425 0.008693 0.011709 0.056062 0.063627 0.042871 0.027042 0.044056 0.059952 0.033365 0.026094
Bud Site 0.082097 0.054798 0.085723 0.074584 0.034229 0.056707 0.082203 0.089780 0.048053 0.056246 0.083182 0.088840
Cell Periphery 0.000561 0.000431 0.000416 0.000239 0.000834 0.000835 0.000665 0.000415 0.000756 0.000738 0.000596 0.000404
Cytoplasm 0.490800* 0.465106* 0.495095* 0.415928* 0.500553* 0.492224* 0.551979* 0.594583* 0.497737* 0.485668* 0.536158* 0.583536*
Cytoplasmic Foci 0.057749 0.220417* 0.062495 0.078785 0.195128* 0.193576* 0.042176 0.041012 0.155454 0.200065* 0.047828 0.043347
Eisosomes 0.000228 0.000542 0.000110 0.000103 0.001945 0.001181 0.000237 0.000219 0.001449 0.001026 0.000202 0.000211
Endoplasmic Reticulum 0.001084 0.000959 0.002960 0.001156 0.002112 0.000843 0.000730 0.000380 0.001815 0.000871 0.001350 0.000428
Endosome 0.007423 0.034306 0.008962 0.006877 0.018189 0.029121 0.003435 0.003819 0.015080 0.030375 0.004972 0.004008
Golgi 0.004505 0.006628 0.000218 0.002358 0.010279 0.010624 0.000639 0.002158 0.008612 0.009658 0.000522 0.002171
Lipid Particles 0.002006 0.004406 0.002586 0.001163 0.015935 0.007729 0.000961 0.000749 0.011912 0.006926 0.001413 0.000774
Mitochondria 0.004237 0.004066 0.000079 0.000073 0.006495 0.004712 0.000584 0.000854 0.005843 0.004555 0.000443 0.000806
Mitotic Spindle 0.011804 0.003206 0.000529 0.003781 0.000800 0.004684 0.004922 0.007361 0.003978 0.004327 0.003700 0.007140
None 0.000442 0.002337 0.001102 0.000361 0.003977 0.000790 0.001027 0.001471 0.002956 0.001164 0.001047 0.001403
Nuclear Periphery 0.000213 0.000195 0.000469 0.000215 0.000324 0.000129 0.000147 0.000106 0.000292 0.000145 0.000237 0.000112
Nuclear Periphery Foci 0.001278 0.000724 0.009729 0.004267 0.000464 0.000268 0.000641 0.000509 0.000699 0.000378 0.003169 0.000741
Nucleolus 0.000151 0.000880 0.000167 0.000062 0.000810 0.000349 0.000130 0.000278 0.000620 0.000477 0.000140 0.000264
Nucleus 0.048010 0.024848 0.080283 0.020779 0.069732 0.028376 0.074497 0.053424 0.063459 0.027523 0.076106 0.051406
Peroxisomes 0.002413 0.011666 0.001334 0.001378 0.005904 0.010028 0.001646 0.002797 0.004896 0.010424 0.001559 0.002709
Vacuole 0.264792* 0.112776 0.237586* 0.370712* 0.073712 0.090462 0.189223 0.168025 0.128894 0.095857 0.202674* 0.180558
Vacuole Periphery 0.001746 0.002204 0.000193 0.004753 0.001471 0.001955 0.000199 0.001131 0.001550 0.002015 0.000197 0.001355

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.72 1.64 2.07 2.66 2.59 -3.53 -0.01 -2.98 -2.00 -2.95 0.20 1.55 -1.85 -2.15 -2.88
Bud Neck -6.45 2.28 2.11 7.49 0.50 -1.78 3.89 10.29 10.95 3.03 -4.77 4.07 8.15 11.63 1.98
Bud Site 1.76 0.80 2.15 1.06 1.20 -5.90 -5.62 -9.20 -3.60 -0.35 -3.42 -4.20 -6.63 -3.62 -0.66
Cell Periphery 0.89 1.77 6.33 5.52 6.31 0.00 2.98 7.91 6.22 3.41 -0.04 3.27 8.31 6.49 3.66
Cytoplasm 0.10 0.71 4.12 3.92 3.33 0.89 -3.55 -9.43 -9.36 -3.45 0.61 -2.63 -9.35 -9.11 -4.71
Cytoplasmic Foci -17.28 -0.63 0.19 14.61 0.57 0.31 32.12 36.40 31.10 1.09 -8.54 27.81 32.28 35.67 2.42
Eisosomes -4.90 5.50 6.77 6.93 2.40 4.66 14.24 14.55 8.76 1.21 3.43 14.31 14.38 9.58 0.29
Endoplasmic Reticulum 0.55 -6.17 -0.07 -0.65 6.38 4.64 5.01 7.03 3.65 3.47 4.49 1.52 7.53 3.92 8.52
Endosome -7.91 -1.96 2.18 8.55 4.05 -6.26 16.16 17.54 16.44 -0.15 -9.92 13.74 16.99 18.48 2.66
Golgi -2.04 2.53 1.78 5.12 -0.88 -0.36 15.09 9.86 8.82 -1.41 -1.65 13.30 7.99 9.05 -1.63
Lipid Particles -2.98 -2.06 1.29 3.93 5.19 6.28 15.09 15.40 8.15 2.08 5.00 14.28 15.36 9.07 6.14
Mitochondria -0.38 2.33 2.36 2.55 1.67 1.75 8.79 8.46 5.11 -0.76 1.09 8.32 7.78 5.49 -1.30
Mitotic Spindle 1.93 3.34 2.35 0.15 -1.27 -3.10 -1.99 -3.86 -0.94 -0.88 -0.69 -0.03 -1.81 -1.12 -1.50
None -1.70 -3.28 -1.45 1.17 0.78 4.23 3.73 3.49 -1.28 -0.68 2.89 3.25 2.93 -0.11 -0.51
Nuclear Periphery 0.38 -6.20 -2.27 -2.19 1.17 3.00 2.59 3.33 0.13 2.22 2.90 0.22 3.31 -0.06 6.80
Nuclear Periphery Foci 1.04 -10.31 -4.57 -5.62 5.58 3.58 -2.28 -1.34 -6.35 1.57 2.46 -9.34 -1.16 -5.99 9.27
Nucleolus -1.61 -0.74 1.47 1.71 2.34 5.74 9.98 6.72 1.63 -2.03 0.95 9.68 5.59 1.96 -1.84
Nucleus 6.53 -5.35 2.83 -1.13 6.62 16.81 -1.00 6.28 -8.35 5.68 17.57 -4.07 5.45 -8.77 7.79
Peroxisomes -6.17 2.05 2.72 7.44 0.59 -4.62 9.46 7.38 8.47 -1.46 -7.22 9.24 5.98 10.23 -1.88
Vacuole 17.49 0.86 -5.96 -12.48 -6.07 -4.54 -18.05 -23.59 -19.27 0.97 9.82 -12.37 -12.58 -20.91 1.49
Vacuole Periphery -1.01 1.66 -0.16 0.36 -0.93 -1.15 11.99 1.76 2.08 -2.07 -1.48 5.89 1.34 2.37 -2.25
Standard name
Human Ortholog
Description Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (64%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Zds1

Zds1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zds1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available