Standard name
Description Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.08 0 0 0
Bud 0.06 0.08 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.13 0 0.11 0.13 0.06 0.06 0.06 0 0.05 0.06 0.12 0.08 0.12 0.12 0.13 0 0.07 0 0 0.07 0.05 0 0
Cytoplasm 0.88 0.87 0.94 0.97 0.92 0.9 0.81 0.83 0.79 0.73 0.7 0.71 0.99 0.99 0.98 0.96 0.96 0.76 0.81 0.66 0.7 0.74 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.18 0.25 0.23 0.33 0.37 0.46 0.46 0 0 0 0 0 0 0 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 2 13 4 16 1 8 4 1 8 3 2 0 0 0 7 3 16 1 28 3 8 11
Bud 11 4 14 8 21 11 25 28 30 37 25 31 0 0 1 5 3 10 3 6 2 4 9
Bud Neck 2 0 2 0 5 3 3 3 0 0 0 0 1 1 0 7 0 8 1 16 5 9 11
Bud Site 1 1 0 0 0 0 0 5 0 2 2 2 0 0 0 0 0
Cell Periphery 26 0 37 41 33 23 43 39 28 43 62 51 14 14 22 18 17 8 0 25 4 7 8
Cytoplasm 173 46 314 309 501 360 569 726 449 492 366 426 115 115 167 411 223 164 59 229 76 150 231
Endoplasmic Reticulum 4 2 1 0 1 4 8 4 5 10 6 3 1 1 2 27 15 3 0 14 2 3 6
Endosome 0 0 2 1 6 2 6 1 1 1 1 5 0 0 0 4 1 0 2 3 0 2 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Mitochondria 0 1 4 7 30 73 173 200 185 247 240 275 0 0 0 14 8 1 1 11 8 8 12
Nucleus 2 1 2 1 1 2 1 3 4 2 1 1 0 0 1 1 1 0 0 0 0 0 1
Nuclear Periphery 0 0 1 0 0 0 0 5 4 4 0 1 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 2 0 0 2 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 3 0 1 0 8 0 3 8 3 5 0 6 0 0 0 2 2 0 0 0 0 0 3
Vac/Vac Membrane 0 0 0 1 7 1 3 8 4 3 1 3 0 0 0 1 1 1 0 1 1 4 7
Unique Cell Count 197 53 335 317 547 401 704 879 568 676 526 602 116 116 171 426 233 215 74 350 109 203 314
Labelled Cell Count 240 57 392 372 631 480 843 1038 714 855 707 806 131 131 193 497 274 215 74 350 109 203 314


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.5 5.0 5.1 5.0 4.3 4.0 4.4 4.1 3.9 4.0 4.0 6.1 6.1 6.2 4.7 5.2 6.0 6.9 6.7 7.0
Std Deviation (1e-4) 0.6 0.4 1.2 1.5 1.7 1.4 1.1 1.2 1.3 1.0 1.1 1.0 0.6 0.6 1.0 1.2 0.9 1.1 1.9 1.4 1.4
Intensity Change (Log2) 0.01 0.0 -0.22 -0.34 -0.2 -0.28 -0.35 -0.35 -0.35 0.28 0.28 0.3 -0.09 0.06 0.26 0.46 0.4 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole