Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 4 |
Bud | 0 | 0 | 1 | 1 | 0 | 2 | 10 | 2 | 14 | 8 | 9 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 5 | 6 | 17 | 18 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 15 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 4 | 3 | 0 | 3 | 1 | 1 | 0 | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 157 | 144 | 137 | 121 | 211 | 207 | 178 | 234 | 218 | 222 | 163 | 184 | 174 | 153 | 197 | 127 | 157 | 180 | 161 | 108 | 170 | 193 | 259 | 252 |
Endoplasmic Reticulum | 3 | 4 | 0 | 1 | 1 | 1 | 0 | 5 | 0 | 5 | 2 | 0 | 2 | 0 | 2 | 10 | 5 | 11 | 19 | 4 | 18 | 4 | 10 | 13 |
Endosome | 2 | 0 | 5 | 9 | 12 | 3 | 9 | 2 | 2 | 0 | 1 | 2 | 4 | 7 | 12 | 7 | 1 | 2 | 12 | 8 | 8 | 7 | 19 | 13 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 1 | 3 | 0 | 0 | 1 | 5 |
Mitochondria | 6 | 0 | 1 | 11 | 14 | 44 | 146 | 59 | 206 | 139 | 178 | 145 | 0 | 0 | 0 | 3 | 3 | 5 | 1 | 0 | 16 | 2 | 5 | 9 |
Nucleus | 9 | 8 | 12 | 2 | 6 | 32 | 30 | 48 | 34 | 65 | 28 | 52 | 21 | 11 | 13 | 2 | 5 | 10 | 36 | 51 | 35 | 41 | 65 | 56 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 15 | 7 | 14 | 6 | 9 | 8 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 2 | 9 | 4 | 9 | 9 |
Vac/Vac Membrane | 124 | 77 | 172 | 118 | 201 | 144 | 152 | 115 | 126 | 103 | 64 | 131 | 203 | 282 | 277 | 102 | 68 | 93 | 22 | 29 | 43 | 32 | 50 | 45 |
Unique Cell Count | 266 | 199 | 288 | 230 | 387 | 367 | 419 | 420 | 482 | 464 | 363 | 434 | 344 | 403 | 436 | 211 | 211 | 271 | 292 | 231 | 338 | 306 | 467 | 463 |
Labelled Cell Count | 303 | 234 | 329 | 266 | 446 | 433 | 527 | 471 | 605 | 549 | 445 | 527 | 405 | 457 | 501 | 254 | 249 | 304 | 292 | 231 | 338 | 306 | 467 | 463 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.7 | 7.5 | 7.2 | 7.6 | 6.0 | 5.4 | 5.8 | 4.9 | 4.9 | 4.6 | 4.9 | 9.3 | 8.7 | 8.8 | 8.5 | 8.6 | 8.1 | 8.3 | 8.1 | 8.8 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.2 | 1.2 | 1.4 | 1.1 | 1.3 | 1.3 | 1.0 | 0.9 | 0.9 | 0.9 | 1.5 | 1.5 | 1.6 | 1.8 | 2.1 | 1.8 | 1.9 | 1.4 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.03 | -0.31 | -0.47 | -0.37 | -0.61 | -0.6 | -0.71 | -0.61 | 0.31 | 0.22 | 0.24 | 0.18 | 0.2 | 0.12 | 0.14 | 0.12 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.8 | 2.2 | -1.3 | 2.1 | -0.6 | 0.1 | -0.7 | -1.4 | 0.8 | -2.5 | -0.6 | 2.8 | 6.0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
Endosome | 1.5 | 1.1 | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 2.9 | 5.8 | 11.1 | 6.4 | 12.8 | 10.1 | 0 | 10.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.3 | -2.1 | 2.3 | 1.7 | 3.4 | 1.6 | 4.3 | 1.9 | 3.6 | 1.1 | -1.0 | -0.9 | -2.2 | -1.1 | -0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -2.0 | -5.2 | -6.1 | -8.6 | -9.3 | -10.4 | -11.1 | -7.9 | -0.2 | 2.8 | 1.0 | -2.5 | -6.1 | -6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.6497 | 9.0024 | 8.1658 | 7.6889 | 6.7235 | 8.0664 | 4.1376 | 6.6423 | 5.7938 | 3.8401 | 4.5192 | 5.2221 | 6.7602 | 7.9435 | 7.2047 | 6.8414 | 6.8742 | 6.9249 |
Actin | 0.0041 | 0.0014 | 0.0068 | 0.0002 | 0.0056 | 0.0005 | 0.0353 | 0.0078 | 0.0064 | 0.0182 | 0.0831 | 0.0219 | 0.0252 | 0.0008 | 0.0237 | 0.002 | 0.0411 | 0.0014 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0005 | 0.0002 | 0.0003 | 0.0011 | 0.0011 | 0.0007 | 0.0028 | 0.001 | 0.0001 | 0.0012 | 0.0001 |
Bud Neck | 0.0018 | 0.0004 | 0.0004 | 0.0003 | 0.0013 | 0.0017 | 0.0057 | 0.0007 | 0.0005 | 0.0018 | 0.0014 | 0.0033 | 0.0056 | 0.0005 | 0.0025 | 0.0004 | 0.0029 | 0.0018 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.002 | 0.0003 | 0.0002 | 0.0005 | 0.0017 | 0.0013 | 0.0013 | 0.0009 | 0.0005 | 0.0001 | 0.0018 | 0.0001 |
Bud Site | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0216 | 0.0025 | 0.0009 | 0.001 | 0.0019 | 0.0018 | 0.0057 | 0.0012 | 0.0011 | 0.0001 | 0.0084 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0012 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0671 | 0.1499 | 0.1106 | 0.0398 | 0.0768 | 0.0728 | 0.1274 | 0.311 | 0.1609 | 0.2293 | 0.055 | 0.0663 | 0.0549 | 0.1836 | 0.0579 | 0.0859 | 0.1177 | 0.0697 |
Cytoplasmic Foci | 0.0048 | 0.0024 | 0.0011 | 0.0002 | 0.0011 | 0.0009 | 0.0296 | 0.015 | 0.0032 | 0.0131 | 0.0211 | 0.0265 | 0.0113 | 0.0004 | 0.003 | 0.0006 | 0.0084 | 0.0013 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0059 | 0.004 | 0.0042 | 0.0018 | 0.006 | 0.0044 | 0.0128 | 0.0051 | 0.0128 | 0.0075 | 0.0144 | 0.006 | 0.0085 | 0.0037 | 0.0054 | 0.0012 | 0.0134 | 0.0024 |
Endosome | 0.0111 | 0.0047 | 0.0035 | 0.0008 | 0.014 | 0.0052 | 0.0478 | 0.0112 | 0.0322 | 0.0457 | 0.0765 | 0.0645 | 0.0294 | 0.0013 | 0.0097 | 0.0018 | 0.0351 | 0.0045 |
Golgi | 0.0015 | 0.0008 | 0.0008 | 0 | 0.0013 | 0.0001 | 0.0176 | 0.0016 | 0.0052 | 0.0263 | 0.1296 | 0.033 | 0.0073 | 0.0001 | 0.0027 | 0.0033 | 0.0248 | 0.0019 |
Lipid Particles | 0.0027 | 0.0002 | 0.0005 | 0 | 0.0014 | 0.0002 | 0.01 | 0.0013 | 0.001 | 0.0207 | 0.0479 | 0.0123 | 0.0057 | 0 | 0.0035 | 0.0154 | 0.0196 | 0.0029 |
Mitochondria | 0.0016 | 0.0005 | 0.0004 | 0.0003 | 0.0051 | 0.0005 | 0.0117 | 0.0024 | 0.0203 | 0.0778 | 0.0199 | 0.0242 | 0.0104 | 0.0009 | 0.0032 | 0.0211 | 0.0213 | 0.0039 |
None | 0.0062 | 0.001 | 0.0014 | 0.0005 | 0.0011 | 0.0012 | 0.0124 | 0.0012 | 0.0013 | 0.0083 | 0.0011 | 0.0012 | 0.0023 | 0.0008 | 0.0008 | 0.0004 | 0.0017 | 0.0006 |
Nuclear Periphery | 0.071 | 0.0427 | 0.0483 | 0.0473 | 0.0859 | 0.0491 | 0.1018 | 0.0614 | 0.0676 | 0.0327 | 0.0648 | 0.0387 | 0.09 | 0.0498 | 0.0461 | 0.0464 | 0.0954 | 0.0531 |
Nucleolus | 0.0016 | 0.0005 | 0.0005 | 0.0008 | 0.001 | 0.0013 | 0.0014 | 0.0007 | 0.0005 | 0.0005 | 0.0007 | 0.0012 | 0.0009 | 0.0006 | 0.0009 | 0.0007 | 0.0024 | 0.0006 |
Nucleus | 0.8022 | 0.7833 | 0.8159 | 0.9008 | 0.7878 | 0.8497 | 0.5366 | 0.5656 | 0.6736 | 0.4991 | 0.4604 | 0.6559 | 0.7106 | 0.7445 | 0.8289 | 0.8138 | 0.5712 | 0.839 |
Peroxisomes | 0.0012 | 0.0014 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0039 | 0.0019 | 0.001 | 0.0053 | 0.0067 | 0.0048 | 0.0126 | 0.0001 | 0.0016 | 0.0029 | 0.0039 | 0.0003 |
Punctate Nuclear | 0.0134 | 0.0035 | 0.0029 | 0.0058 | 0.0069 | 0.0089 | 0.0081 | 0.0063 | 0.0025 | 0.0035 | 0.0069 | 0.0279 | 0.0092 | 0.0065 | 0.0036 | 0.0026 | 0.0224 | 0.0122 |
Vacuole | 0.0019 | 0.0021 | 0.0016 | 0.0008 | 0.0024 | 0.0024 | 0.0101 | 0.0026 | 0.0071 | 0.005 | 0.0028 | 0.0048 | 0.0042 | 0.0012 | 0.0026 | 0.0005 | 0.0038 | 0.0032 |
Vacuole Periphery | 0.0008 | 0.0004 | 0.0004 | 0.0005 | 0.0015 | 0.0006 | 0.0016 | 0.0009 | 0.0024 | 0.0025 | 0.0024 | 0.0031 | 0.0023 | 0.0003 | 0.001 | 0.0005 | 0.0028 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 163.2488 | 166.1543 | 173.5244 | 177.102 | 169.5824 | 190.6791 | 206.4425 | 208.1772 | 231.5825 | 158.9429 |
Translational Efficiency | 2.6398 | 2.5422 | 2.3608 | 2.2203 | 2.1747 | 2.8028 | 1.9445 | 2.458 | 2.0271 | 2.1763 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
32 | 153 | 2089 | 1316 | 1396 | 152 | 134 | 1164 | 1428 | 305 | 2223 | 2480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1278.33 | 1053.61 | 1174.36 | 1124.01 | 919.93 | 999.86 | 1298.97 | 1169.01 | 927.96 | 1026.82 | 1181.87 | 1145.13 |
Standard Deviation | 368.32 | 151.44 | 159.35 | 184.59 | 120.96 | 119.37 | 199.84 | 172.82 | 141.97 | 139.03 | 164.77 | 180.57 |
Intensity Change Log 2 | — | -0.278919 | -0.122386 | -0.185605 | — | 0.120202 | 0.497772 | 0.345691 | — | -0.098298 | 0.170093 | 0.060887 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.017607 | 0.001264 | 0.000369 | 0.000834 | 0.000241 | 0.000692 | 0.001417 | 0.004897 | 0.000631 | 0.000979 | 0.000432 | 0.002741 |
Bud Neck | 0.014042 | 0.005962 | 0.008053 | 0.006652 | 0.003428 | 0.004098 | 0.006044 | 0.009398 | 0.003665 | 0.005033 | 0.007932 | 0.007941 |
Bud Site | 0.019207 | 0.007065 | 0.002798 | 0.022866 | 0.003247 | 0.006297 | 0.006516 | 0.054934 | 0.003605 | 0.006682 | 0.003022 | 0.037917 |
Cell Periphery | 0.000707 | 0.000212 | 0.000100 | 0.000240 | 0.000106 | 0.000133 | 0.000369 | 0.000159 | 0.000119 | 0.000173 | 0.000117 | 0.000202 |
Cytoplasm | 0.145225* | 0.465588* | 0.214498* | 0.551545* | 0.514671* | 0.517683* | 0.259399* | 0.516496* | 0.506393* | 0.491550* | 0.217204* | 0.535094* |
Cytoplasmic Foci | 0.035494 | 0.072053 | 0.000763 | 0.012167 | 0.068346 | 0.121697 | 0.007038 | 0.023943 | 0.067610 | 0.096794 | 0.001141 | 0.017694 |
Eisosomes | 0.000231 | 0.000066 | 0.000011 | 0.000019 | 0.000044 | 0.000067 | 0.000085 | 0.000022 | 0.000048 | 0.000066 | 0.000016 | 0.000021 |
Endoplasmic Reticulum | 0.038076 | 0.030006 | 0.010668 | 0.004065 | 0.017966 | 0.009413 | 0.019819 | 0.001677 | 0.018416 | 0.019743 | 0.011220 | 0.002944 |
Endosome | 0.023669 | 0.012051 | 0.001122 | 0.004170 | 0.007135 | 0.016831 | 0.007811 | 0.009477 | 0.007505 | 0.014433 | 0.001525 | 0.006660 |
Golgi | 0.002188 | 0.000958 | 0.000052 | 0.003467 | 0.001076 | 0.001981 | 0.000229 | 0.016665 | 0.001101 | 0.001467 | 0.000063 | 0.009662 |
Lipid Particles | 0.037428 | 0.008099 | 0.000611 | 0.002165 | 0.007562 | 0.006812 | 0.004706 | 0.001511 | 0.008231 | 0.007458 | 0.000858 | 0.001858 |
Mitochondria | 0.002000 | 0.000378 | 0.002253 | 0.000930 | 0.001273 | 0.000405 | 0.000818 | 0.006138 | 0.001290 | 0.000392 | 0.002166 | 0.003374 |
Mitotic Spindle | 0.009520 | 0.032405 | 0.012267 | 0.026196 | 0.012561 | 0.009642 | 0.010193 | 0.112093* | 0.012493 | 0.021061 | 0.012142 | 0.066512 |
None | 0.000894 | 0.002648 | 0.005545 | 0.011011 | 0.002958 | 0.002092 | 0.002977 | 0.012880 | 0.002912 | 0.002371 | 0.005391 | 0.011888 |
Nuclear Periphery | 0.023194 | 0.007564 | 0.021390 | 0.001475 | 0.016787 | 0.007770 | 0.006822 | 0.001186 | 0.016931 | 0.007666 | 0.020512 | 0.001339 |
Nuclear Periphery Foci | 0.093227 | 0.034948 | 0.006284 | 0.011479 | 0.017025 | 0.023275 | 0.023561 | 0.003891 | 0.018732 | 0.029131 | 0.007325 | 0.007917 |
Nucleolus | 0.026069 | 0.000726 | 0.000637 | 0.000212 | 0.000864 | 0.000399 | 0.000864 | 0.000532 | 0.001429 | 0.000563 | 0.000651 | 0.000362 |
Nucleus | 0.293561* | 0.136167* | 0.689395* | 0.265518* | 0.249831* | 0.128916 | 0.371768* | 0.145224* | 0.250810* | 0.132553* | 0.670248* | 0.209058* |
Peroxisomes | 0.007269 | 0.001444 | 0.000066 | 0.000476 | 0.000734 | 0.001735 | 0.000303 | 0.002498 | 0.000880 | 0.001589 | 0.000080 | 0.001425 |
Vacuole | 0.192575 | 0.179810 | 0.022979 | 0.072828 | 0.073274 | 0.139451 | 0.268981* | 0.072923 | 0.075947 | 0.159697 | 0.037808 | 0.072873 |
Vacuole Periphery | 0.017816 | 0.000586 | 0.000140 | 0.001685 | 0.000872 | 0.000608 | 0.000282 | 0.003457 | 0.001252 | 0.000597 | 0.000149 | 0.002517 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.03 | 1.11 | 1.09 | 1.17 | -4.07 | -2.31 | -9.82 | -3.52 | -1.96 | 0.81 | -1.20 | -0.10 | -1.46 | 0.13 | -3.02 |
Bud Neck | 1.32 | 0.50 | 1.53 | 0.32 | 5.55 | -1.60 | -5.18 | -4.97 | -2.23 | 1.73 | -2.05 | -9.72 | -5.21 | -0.61 | 6.09 |
Bud Site | 0.97 | 1.48 | 0.56 | -2.18 | -6.01 | -3.35 | -6.03 | -7.32 | -5.17 | -4.81 | -4.58 | -0.29 | -8.89 | -5.31 | -9.01 |
Cell Periphery | 2.27 | 2.65 | 2.11 | -1.37 | -6.25 | -2.33 | -9.60 | -6.43 | -1.43 | 7.66 | -3.88 | -2.84 | -6.92 | -2.01 | -5.40 |
Cytoplasm | -6.25 | -0.71 | -7.64 | -0.78 | -33.46 | 1.25 | 20.18 | 9.04 | 2.59 | -14.33 | 2.72 | 38.94 | 7.97 | 1.33 | -39.04 |
Cytoplasmic Foci | -3.25 | 4.35 | 2.83 | 7.89 | -14.49 | -3.56 | 21.62 | 18.19 | 7.81 | -7.49 | -3.10 | 23.94 | 19.08 | 10.52 | -19.59 |
Eisosomes | 2.14 | 2.84 | 2.68 | 5.52 | -7.97 | -3.10 | -7.50 | 4.14 | 4.36 | 9.06 | -3.65 | 9.03 | 5.77 | 6.99 | -6.93 |
Endoplasmic Reticulum | 1.57 | 2.95 | 3.98 | 3.26 | 13.75 | 3.94 | -1.32 | 11.63 | 4.73 | 9.99 | 0.70 | 3.37 | 11.22 | 4.38 | 18.38 |
Endosome | 2.02 | 3.57 | 3.50 | 7.04 | -1.09 | -1.84 | -0.15 | 7.03 | 2.94 | 5.71 | -2.43 | 11.16 | 9.26 | 4.92 | -1.28 |
Golgi | 1.44 | 2.11 | 1.09 | -0.65 | -2.33 | -0.41 | 1.75 | -1.72 | -1.00 | -2.58 | -0.07 | 2.27 | -1.31 | -1.11 | -3.44 |
Lipid Particles | 1.44 | 1.79 | 1.68 | 4.83 | -9.66 | 0.62 | 3.90 | 9.42 | 3.39 | 4.47 | 0.62 | 10.24 | 7.35 | 5.68 | -10.98 |
Mitochondria | 1.99 | -1.17 | 1.57 | -4.57 | 19.24* | 3.32 | 0.29 | -0.61 | -2.08 | -0.78 | 3.55 | -6.03 | 0.27 | -2.68 | 5.58 |
Mitotic Spindle | -1.79 | -1.21 | -0.88 | 1.51 | 0.80 | 0.99 | -0.60 | -5.91 | -5.51 | -2.56 | -0.98 | -1.61 | -4.52 | -1.93 | -3.36 |
None | -3.05 | -13.24 | -8.30 | -6.12 | -1.92 | 1.20 | 0.73 | -9.24 | -9.34 | -9.24 | 1.34 | -6.40 | -9.52 | -10.88 | -5.66 |
Nuclear Periphery | 1.15 | -0.23 | 1.46 | 2.70 | 16.54 | 3.65 | 4.41 | 10.49 | 1.96 | 5.50 | 5.33 | -3.72 | 10.69 | 3.13 | 16.72 |
Nuclear Periphery Foci | 2.47 | 3.70 | 3.59 | 4.62 | -1.73 | -1.34 | -0.95 | 8.62 | 4.31 | 4.98 | -2.45 | 6.29 | 7.20 | 6.26 | 1.18 |
Nucleolus | 1.85 | 1.82 | 1.89 | 2.45 | 7.14 | 5.22 | 0.27 | 4.55 | -0.56 | 1.27 | 2.51 | 0.76 | 3.40 | 2.15 | 6.50 |
Nucleus | 3.02 | -7.37 | 0.19 | -12.20 | 51.44 | 10.11 | -5.17 | 5.49 | -6.76 | 6.79 | 12.62 | -54.18 | 0.19 | -13.64 | 62.93 |
Peroxisomes | 1.62 | 2.03 | 1.98 | 3.82 | -2.23 | -2.69 | 4.57 | 0.17 | 2.49 | -1.87 | -2.78 | 6.47 | 2.69 | 4.45 | -3.37 |
Vacuole | -1.29 | 3.83 | -0.17 | 2.29 | -21.17 | -6.29 | -10.15 | -20.07 | -3.76 | 2.77 | -9.54 | 4.70 | -21.39 | -0.72 | -27.09 |
Vacuole Periphery | 2.18 | 2.21 | 2.17 | -0.25 | -1.41 | 0.85 | 2.04 | 0.37 | -0.41 | -1.17 | 2.09 | 3.29 | 1.64 | -0.46 | -2.09 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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