Standard name
Human Ortholog
Description Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.72 0.48 0.53 0.55 0.56 0.42 0.56 0.45 0.48 0.45 0.42 0.51 0.38 0.45 0.6 0.74 0.66 0.55 0.47 0.5 0.63 0.56 0.55
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.12 0.35 0.14 0.43 0.3 0.49 0.33 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0.09 0.07 0.11 0.07 0.14 0.08 0.12 0.06 0 0 0 0 0 0.13 0.22 0.1 0.14 0.14 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.39 0.6 0.51 0.52 0.39 0.36 0.27 0.26 0.22 0.18 0.3 0.59 0.7 0.64 0.48 0.32 0.34 0.08 0.13 0.13 0.11 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 1 0 0 2 4
Bud 0 0 1 1 0 2 10 2 14 8 9 6 0 0 0 0 0 0 4 7 5 6 17 18
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 4 15
Bud Site 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 3 4 3 0 3 1 1 0 3 6 1 0 0 0 0 0 0
Cytoplasm 157 144 137 121 211 207 178 234 218 222 163 184 174 153 197 127 157 180 161 108 170 193 259 252
Endoplasmic Reticulum 3 4 0 1 1 1 0 5 0 5 2 0 2 0 2 10 5 11 19 4 18 4 10 13
Endosome 2 0 5 9 12 3 9 2 2 0 1 2 4 7 12 7 1 2 12 8 8 7 19 13
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 4 2 1 3 0 0 1 5
Mitochondria 6 0 1 11 14 44 146 59 206 139 178 145 0 0 0 3 3 5 1 0 16 2 5 9
Nucleus 9 8 12 2 6 32 30 48 34 65 28 52 21 11 13 2 5 10 36 51 35 41 65 56
Nuclear Periphery 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 15 7 14 6 9 8
Nucleolus 1 0 0 1 0 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 4 2 9 4 9 9
Vac/Vac Membrane 124 77 172 118 201 144 152 115 126 103 64 131 203 282 277 102 68 93 22 29 43 32 50 45
Unique Cell Count 266 199 288 230 387 367 419 420 482 464 363 434 344 403 436 211 211 271 292 231 338 306 467 463
Labelled Cell Count 303 234 329 266 446 433 527 471 605 549 445 527 405 457 501 254 249 304 292 231 338 306 467 463


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.7 7.5 7.2 7.6 6.0 5.4 5.8 4.9 4.9 4.6 4.9 9.3 8.7 8.8 8.5 8.6 8.1 8.3 8.1 8.8
Std Deviation (1e-4) 0.8 1.2 1.2 1.2 1.4 1.1 1.3 1.3 1.0 0.9 0.9 0.9 1.5 1.5 1.6 1.8 2.1 1.8 1.9 1.4 2.0
Intensity Change (Log2) – – – -0.06 0.03 -0.31 -0.47 -0.37 -0.61 -0.6 -0.71 -0.61 0.31 0.22 0.24 0.18 0.2 0.12 0.14 0.12 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.5 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.8 2.2 -1.3 2.1 -0.6 0.1 -0.7 -1.4 0.8 -2.5 -0.6 2.8 6.0 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Endosome 1.5 1.1 0 0.4 0 0 0 0 0 0 0 0.9 1.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 2.9 5.8 11.1 6.4 12.8 10.1 0 10.8 0 0 0 0 0 0
Nucleus -2.3 -2.1 2.3 1.7 3.4 1.6 4.3 1.9 3.6 1.1 -1.0 -0.9 -2.2 -1.1 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -2.0 -5.2 -6.1 -8.6 -9.3 -10.4 -11.1 -7.9 -0.2 2.8 1.0 -2.5 -6.1 -6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6497 9.0024 8.1658 7.6889 6.7235 8.0664 4.1376 6.6423 5.7938 3.8401 4.5192 5.2221 6.7602 7.9435 7.2047 6.8414 6.8742 6.9249
Actin 0.0041 0.0014 0.0068 0.0002 0.0056 0.0005 0.0353 0.0078 0.0064 0.0182 0.0831 0.0219 0.0252 0.0008 0.0237 0.002 0.0411 0.0014
Bud 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0019 0.0005 0.0002 0.0003 0.0011 0.0011 0.0007 0.0028 0.001 0.0001 0.0012 0.0001
Bud Neck 0.0018 0.0004 0.0004 0.0003 0.0013 0.0017 0.0057 0.0007 0.0005 0.0018 0.0014 0.0033 0.0056 0.0005 0.0025 0.0004 0.0029 0.0018
Bud Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.002 0.0003 0.0002 0.0005 0.0017 0.0013 0.0013 0.0009 0.0005 0.0001 0.0018 0.0001
Bud Site 0.0005 0.0004 0.0002 0.0001 0.0001 0.0002 0.0216 0.0025 0.0009 0.001 0.0019 0.0018 0.0057 0.0012 0.0011 0.0001 0.0084 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0004 0.0001 0.0001 0.0003 0.0003 0.0002 0.0012 0 0.0001 0 0.0004 0
Cytoplasm 0.0671 0.1499 0.1106 0.0398 0.0768 0.0728 0.1274 0.311 0.1609 0.2293 0.055 0.0663 0.0549 0.1836 0.0579 0.0859 0.1177 0.0697
Cytoplasmic Foci 0.0048 0.0024 0.0011 0.0002 0.0011 0.0009 0.0296 0.015 0.0032 0.0131 0.0211 0.0265 0.0113 0.0004 0.003 0.0006 0.0084 0.0013
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0006 0.0003 0.0001 0.0006 0 0.0002 0.0001 0.0005 0
Endoplasmic Reticulum 0.0059 0.004 0.0042 0.0018 0.006 0.0044 0.0128 0.0051 0.0128 0.0075 0.0144 0.006 0.0085 0.0037 0.0054 0.0012 0.0134 0.0024
Endosome 0.0111 0.0047 0.0035 0.0008 0.014 0.0052 0.0478 0.0112 0.0322 0.0457 0.0765 0.0645 0.0294 0.0013 0.0097 0.0018 0.0351 0.0045
Golgi 0.0015 0.0008 0.0008 0 0.0013 0.0001 0.0176 0.0016 0.0052 0.0263 0.1296 0.033 0.0073 0.0001 0.0027 0.0033 0.0248 0.0019
Lipid Particles 0.0027 0.0002 0.0005 0 0.0014 0.0002 0.01 0.0013 0.001 0.0207 0.0479 0.0123 0.0057 0 0.0035 0.0154 0.0196 0.0029
Mitochondria 0.0016 0.0005 0.0004 0.0003 0.0051 0.0005 0.0117 0.0024 0.0203 0.0778 0.0199 0.0242 0.0104 0.0009 0.0032 0.0211 0.0213 0.0039
None 0.0062 0.001 0.0014 0.0005 0.0011 0.0012 0.0124 0.0012 0.0013 0.0083 0.0011 0.0012 0.0023 0.0008 0.0008 0.0004 0.0017 0.0006
Nuclear Periphery 0.071 0.0427 0.0483 0.0473 0.0859 0.0491 0.1018 0.0614 0.0676 0.0327 0.0648 0.0387 0.09 0.0498 0.0461 0.0464 0.0954 0.0531
Nucleolus 0.0016 0.0005 0.0005 0.0008 0.001 0.0013 0.0014 0.0007 0.0005 0.0005 0.0007 0.0012 0.0009 0.0006 0.0009 0.0007 0.0024 0.0006
Nucleus 0.8022 0.7833 0.8159 0.9008 0.7878 0.8497 0.5366 0.5656 0.6736 0.4991 0.4604 0.6559 0.7106 0.7445 0.8289 0.8138 0.5712 0.839
Peroxisomes 0.0012 0.0014 0.0003 0 0.0005 0.0001 0.0039 0.0019 0.001 0.0053 0.0067 0.0048 0.0126 0.0001 0.0016 0.0029 0.0039 0.0003
Punctate Nuclear 0.0134 0.0035 0.0029 0.0058 0.0069 0.0089 0.0081 0.0063 0.0025 0.0035 0.0069 0.0279 0.0092 0.0065 0.0036 0.0026 0.0224 0.0122
Vacuole 0.0019 0.0021 0.0016 0.0008 0.0024 0.0024 0.0101 0.0026 0.0071 0.005 0.0028 0.0048 0.0042 0.0012 0.0026 0.0005 0.0038 0.0032
Vacuole Periphery 0.0008 0.0004 0.0004 0.0005 0.0015 0.0006 0.0016 0.0009 0.0024 0.0025 0.0024 0.0031 0.0023 0.0003 0.001 0.0005 0.0028 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 163.2488 166.1543 173.5244 177.102 169.5824 190.6791 206.4425 208.1772 231.5825 158.9429
Translational Efficiency 2.6398 2.5422 2.3608 2.2203 2.1747 2.8028 1.9445 2.458 2.0271 2.1763

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (26%), ER (20%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dsk2

Dsk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dsk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available