Standard name
Description Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.72 0.48 0.53 0.55 0.56 0.42 0.56 0.45 0.48 0.45 0.42 0.51 0.38 0.45 0.6 0.74 0.66 0.55 0.47 0.5 0.63 0.56 0.55
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.12 0.35 0.14 0.43 0.3 0.49 0.33 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0.09 0.07 0.11 0.07 0.14 0.08 0.12 0.06 0 0 0 0 0 0.13 0.22 0.1 0.14 0.14 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.39 0.6 0.51 0.52 0.39 0.36 0.27 0.26 0.22 0.18 0.3 0.59 0.7 0.64 0.48 0.32 0.34 0.08 0.13 0.13 0.11 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 1 0 0 2 4
Bud 0 0 1 1 0 2 10 2 14 8 9 6 0 0 0 0 0 0 4 7 5 6 17 18
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 4 15
Bud Site 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 3 4 3 0 3 1 1 0 3 6 1 0 0 0 0 0 0
Cytoplasm 157 144 137 121 211 207 178 234 218 222 163 184 174 153 197 127 157 180 161 108 170 193 259 252
Endoplasmic Reticulum 3 4 0 1 1 1 0 5 0 5 2 0 2 0 2 10 5 11 19 4 18 4 10 13
Endosome 2 0 5 9 12 3 9 2 2 0 1 2 4 7 12 7 1 2 12 8 8 7 19 13
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 4 2 1 3 0 0 1 5
Mitochondria 6 0 1 11 14 44 146 59 206 139 178 145 0 0 0 3 3 5 1 0 16 2 5 9
Nucleus 9 8 12 2 6 32 30 48 34 65 28 52 21 11 13 2 5 10 36 51 35 41 65 56
Nuclear Periphery 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 15 7 14 6 9 8
Nucleolus 1 0 0 1 0 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 4 2 9 4 9 9
Vac/Vac Membrane 124 77 172 118 201 144 152 115 126 103 64 131 203 282 277 102 68 93 22 29 43 32 50 45
Unique Cell Count 266 199 288 230 387 367 419 420 482 464 363 434 344 403 436 211 211 271 292 231 338 306 467 463
Labelled Cell Count 303 234 329 266 446 433 527 471 605 549 445 527 405 457 501 254 249 304 292 231 338 306 467 463


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.7 7.5 7.2 7.6 6.0 5.4 5.8 4.9 4.9 4.6 4.9 9.3 8.7 8.8 8.5 8.6 8.1 8.3 8.1 8.8
Std Deviation (1e-4) 0.8 1.2 1.2 1.2 1.4 1.1 1.3 1.3 1.0 0.9 0.9 0.9 1.5 1.5 1.6 1.8 2.1 1.8 1.9 1.4 2.0
Intensity Change (Log2) – – – -0.06 0.03 -0.31 -0.47 -0.37 -0.61 -0.6 -0.71 -0.61 0.31 0.22 0.24 0.18 0.2 0.12 0.14 0.12 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.5 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.8 2.2 -1.3 2.1 -0.6 0.1 -0.7 -1.4 0.8 -2.5 -0.6 2.8 6.0 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Endosome 1.5 1.1 0 0.4 0 0 0 0 0 0 0 0.9 1.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 2.9 5.8 11.1 6.4 12.8 10.1 0 10.8 0 0 0 0 0 0
Nucleus -2.3 -2.1 2.3 1.7 3.4 1.6 4.3 1.9 3.6 1.1 -1.0 -0.9 -2.2 -1.1 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -2.0 -5.2 -6.1 -8.6 -9.3 -10.4 -11.1 -7.9 -0.2 2.8 1.0 -2.5 -6.1 -6.0