Standard name | |
---|---|
Description | Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 3 |
Bud | 0 | 0 | 2 | 0 | 0 | 0 | 7 | 8 | – | 7 | 11 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 12 | 9 | 7 | 5 | 13 | 7 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 305 | 342 | 271 | 190 | 180 | 292 | 396 | 378 | – | 505 | 395 | 509 | 330 | 365 | 446 | 217 | 176 | 157 | 60 | 81 | 62 | 42 | 38 | 43 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | – | 2 | 2 | 3 | 7 | 2 | 4 | 22 | 9 | 14 | 2 | 2 | 3 | 1 | 3 | 5 |
Endosome | 20 | 3 | 7 | 0 | 14 | 0 | 0 | 2 | – | 3 | 0 | 1 | 1 | 3 | 1 | 4 | 7 | 3 | 26 | 13 | 8 | 3 | 5 | 10 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 3 |
Mitochondria | 21 | 12 | 22 | 6 | 16 | 44 | 65 | 92 | – | 91 | 90 | 131 | 0 | 0 | 0 | 9 | 2 | 1 | 7 | 6 | 11 | 6 | 10 | 14 |
Nucleus | 23 | 22 | 11 | 12 | 32 | 40 | 27 | 36 | – | 27 | 18 | 18 | 6 | 2 | 1 | 55 | 43 | 35 | 4 | 2 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 10 | 4 | 10 | 8 | – | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | 1 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 8 | 6 | 1 | 0 | 2 | 3 |
Vac/Vac Membrane | 226 | 208 | 116 | 102 | 205 | 196 | 201 | 204 | – | 216 | 151 | 135 | 42 | 33 | 10 | 152 | 135 | 118 | 438 | 409 | 276 | 166 | 225 | 220 |
Unique Cell Count | 515 | 514 | 368 | 246 | 362 | 458 | 574 | 619 | 711 | 535 | 656 | 374 | 398 | 458 | 367 | 299 | 259 | 570 | 546 | 387 | 234 | 313 | 321 | |
Labelled Cell Count | 601 | 588 | 432 | 313 | 457 | 577 | 710 | 730 | 857 | 673 | 810 | 391 | 406 | 464 | 464 | 373 | 328 | 570 | 546 | 387 | 234 | 313 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.6 | 4.3 | 4.4 | 4.9 | 4.8 | 4.1 | 4.2 | – | 4.2 | 4.2 | 4.1 | 5.5 | 5.2 | 5.1 | 6.0 | 6.0 | 6.1 | 6.3 | 6.3 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.4 | 1.6 | 1.9 | 1.6 | 1.5 | 1.1 | – | 1.3 | 1.3 | 1.2 | 1.0 | 1.0 | 1.0 | 1.3 | 1.5 | 1.4 | 1.5 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.18 | 0.14 | -0.06 | -0.03 | – | -0.02 | -0.02 | -0.07 | 0.36 | 0.28 | 0.25 | 0.47 | 0.49 | 0.5 | 0.55 | 0.56 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.8 | 3.6 | 3.6 |