Standard name
Description Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.67 0.74 0.77 0.5 0.64 0.69 0.61 – 0.71 0.74 0.78 0.88 0.92 0.97 0.59 0.59 0.61 0.11 0.15 0.16 0.18 0.12 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0.1 0.11 0.15 – 0.13 0.17 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.05 0.09 0.09 0.05 0.06 – 0 0 0 0 0 0 0.15 0.14 0.14 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.44 0.4 0.32 0.41 0.57 0.43 0.35 0.33 – 0.3 0.28 0.21 0.11 0.08 0 0.41 0.45 0.46 0.77 0.75 0.71 0.71 0.72 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 2 4 0 0 3
Bud 0 0 2 0 0 0 7 8 – 7 11 7 1 0 0 0 0 0 12 9 7 5 13 7
Bud Neck 1 0 0 0 0 0 0 0 – 0 0 0 2 0 1 0 0 0 0 1 0 1 2 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 – 1 0 0 2 1 1 1 0 0 0 0 0 0 0 0
Cytoplasm 305 342 271 190 180 292 396 378 – 505 395 509 330 365 446 217 176 157 60 81 62 42 38 43
Endoplasmic Reticulum 2 0 0 0 0 1 4 0 – 2 2 3 7 2 4 22 9 14 2 2 3 1 3 5
Endosome 20 3 7 0 14 0 0 2 – 3 0 1 1 3 1 4 7 3 26 13 8 3 5 10
Golgi 3 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 3 1 1 1 0 3
Mitochondria 21 12 22 6 16 44 65 92 – 91 90 131 0 0 0 9 2 1 7 6 11 6 10 14
Nucleus 23 22 11 12 32 40 27 36 – 27 18 18 6 2 1 55 43 35 4 2 0 1 1 0
Nuclear Periphery 0 0 0 3 10 4 10 8 – 3 3 4 0 0 0 0 1 0 1 0 0 0 2 0
Nucleolus 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 – 1 1 2 0 0 0 2 0 0 8 6 1 0 2 3
Vac/Vac Membrane 226 208 116 102 205 196 201 204 – 216 151 135 42 33 10 152 135 118 438 409 276 166 225 220
Unique Cell Count 515 514 368 246 362 458 574 619 711 535 656 374 398 458 367 299 259 570 546 387 234 313 321
Labelled Cell Count 601 588 432 313 457 577 710 730 857 673 810 391 406 464 464 373 328 570 546 387 234 313 321


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 4.3 4.4 4.9 4.8 4.1 4.2 – 4.2 4.2 4.1 5.5 5.2 5.1 6.0 6.0 6.1 6.3 6.3 6.2
Std Deviation (1e-4) 0.8 0.9 1.4 1.6 1.9 1.6 1.5 1.1 – 1.3 1.3 1.2 1.0 1.0 1.0 1.3 1.5 1.4 1.5 1.7 1.7
Intensity Change (Log2) – – – 0.02 0.18 0.14 -0.06 -0.03 – -0.02 -0.02 -0.07 0.36 0.28 0.25 0.47 0.49 0.5 0.55 0.56 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 3.6 3.6