Standard name
Description Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.05 – 0 0.15 0.1 0.13 – 0.06 0.08 0.11 0.07 – – – 0.07 0.07 0 0.06 0 0.05 0 0.15 0.24
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0.21 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0 – 0.08 0 0.2 0.29 – 0.45 0.56 0.7 0.58 – – – 0 0 0 0.05 0 0.05 0 0 0
Nucleus 0.6 0.73 – 0.62 0.62 0.66 0.61 – 0.73 0.68 0.48 0.64 – – – 0.83 0.84 0.84 0.51 0.71 0.43 0 0.41 0.29
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.1 0.28 – 0.08 0.19 0.15 0.08 – 0.06 0.05 0.05 0 – – – 0.1 0.1 0.12 0.16 0.12 0.29 0 0.3 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Bud 0 0 – 0 0 1 0 – 1 1 3 2 – – – 0 2 0 2 5 1 0 0 0
Bud Neck 0 0 – 0 2 1 1 – 0 1 1 0 – – – 0 0 0 1 0 0 0 0 0
Bud Site 1 0 – 0 0 0 0 – 0 1 2 3 – – – 0 0 1 – – – – – –
Cell Periphery 0 1 – 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Cytoplasm 17 12 – 1 11 12 13 – 3 9 14 7 – – – 26 22 14 11 4 2 0 1 1
Endoplasmic Reticulum 0 1 – 0 0 0 0 – 0 0 0 1 – – – 8 10 8 1 0 0 0 0 0
Endosome 2 2 – 5 1 1 0 – 0 0 0 0 – – – 0 1 5 9 7 1 0 0 0
Golgi 0 1 – 0 0 0 0 – 0 0 0 0 – – – 1 1 3 0 2 0 0 0 0
Mitochondria 69 4 – 2 2 23 30 – 22 61 88 60 – – – 11 4 1 9 5 2 0 0 0
Nucleus 105 182 – 15 46 76 62 – 36 73 60 67 – – – 294 280 267 106 195 23 0 4 1
Nuclear Periphery 1 1 – 0 0 0 0 – 0 0 1 2 – – – 1 3 1 6 2 3 0 0 0
Nucleolus 3 0 – 1 0 0 1 – 0 0 0 1 – – – 1 1 1 5 6 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
SpindlePole 0 3 – 0 1 1 0 – 1 0 0 0 – – – 1 0 0 11 6 1 0 0 0
Vac/Vac Membrane 17 70 – 2 14 17 8 – 3 5 6 4 – – – 37 33 37 34 32 15 0 3 0
Unique Cell Count 176 248 24 74 116 102 49 108 126 104 353 332 319 209 275 54 1 12 6
Labelled Cell Count 215 277 26 77 132 115 66 151 175 147 381 358 338 209 275 54 1 12 6


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.6 – 5.9 6.1 4.9 5.1 – 3.7 3.9 3.7 3.6 – – – 8.5 8.5 8.6 – – –
Std Deviation (1e-4) 0.7 1.0 – 2.0 1.9 1.4 1.9 – 0.4 0.8 0.8 0.7 – – – 1.7 1.8 1.8 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –