Standard name
Description Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.95 1.0 0.94 0.89 0.77 0.82 0.73 0.68 0.56 0.7 1.0 0.99 0.99 0.98 0.96 0.96 0.91 0.95 0.94 0.93 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.08 0.1 0.1 0.3 0.48 0.38 0.5 0.56 0.72 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 4
Bud 1 2 5 0 4 1 2 3 1 4 0 5 1 1 1 1 0 0 0 0 0 0 3 6
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 0 0 0 3 1 1 0 0 1 1 1 5 3 8 11 7 0 0 0 0 0 1
Cytoplasm 224 237 113 41 126 149 123 187 80 86 92 109 472 510 515 558 570 494 223 230 111 227 272 305
Endoplasmic Reticulum 1 1 0 0 1 2 1 0 0 0 0 0 1 2 1 27 22 22 1 0 0 2 1 6
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 4 7 3 0 0 2 6 5
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 2 1 0 0 1 0
Mitochondria 11 0 9 4 14 51 77 87 55 71 119 81 0 2 1 5 6 3 1 3 2 2 6 9
Nucleus 0 0 0 0 0 1 1 1 1 0 4 1 0 1 2 3 5 3 0 1 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 1 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 1 0 2 0 0 0 2 1 0 1 0 0 0 6 7
Vac/Vac Membrane 0 0 0 0 0 0 1 0 0 0 2 1 1 0 1 10 22 9 7 1 0 3 6 11
Unique Cell Count 233 240 119 41 134 168 160 227 109 126 165 156 473 516 520 569 593 512 245 243 118 245 312 365
Labelled Cell Count 243 245 129 45 145 207 207 281 139 164 219 201 476 524 524 616 644 546 245 243 118 245 312 365


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 4.6 4.3 4.2 3.6 3.0 3.4 3.4 3.5 3.1 3.2 6.1 5.8 5.8 5.8 7.0 6.4 5.0 4.8 5.6
Std Deviation (1e-4) 0.7 0.7 0.8 0.6 1.3 0.8 0.7 1.0 0.8 1.8 0.9 1.3 0.9 1.1 1.2 1.2 1.4 1.1 0.9 1.0 1.3
Intensity Change (Log2) – – – -0.1 -0.14 -0.38 -0.65 -0.45 -0.46 -0.42 -0.57 -0.55 0.39 0.33 0.31 0.31 0.58 0.47 0.1 0.05 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.3 -1.9 -4.1 -3.3 -4.5 -5.3 -7.3 -5.2 4.4 2.8 3.1 2.0 0.6 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0