Standard name
Human Ortholog
Description Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.95 1.0 0.94 0.89 0.77 0.82 0.73 0.68 0.56 0.7 1.0 0.99 0.99 0.98 0.96 0.96 0.91 0.95 0.94 0.93 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.08 0.1 0.1 0.3 0.48 0.38 0.5 0.56 0.72 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 4
Bud 1 2 5 0 4 1 2 3 1 4 0 5 1 1 1 1 0 0 0 0 0 0 3 6
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0
Cell Periphery 2 3 0 0 0 3 1 1 0 0 1 1 1 5 3 8 11 7 0 0 0 0 0 1
Cytoplasm 224 237 113 41 126 149 123 187 80 86 92 109 472 510 515 558 570 494 223 230 111 227 272 305
Endoplasmic Reticulum 1 1 0 0 1 2 1 0 0 0 0 0 1 2 1 27 22 22 1 0 0 2 1 6
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 4 7 3 0 0 2 6 5
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1 2 1 0 0 1 0
Mitochondria 11 0 9 4 14 51 77 87 55 71 119 81 0 2 1 5 6 3 1 3 2 2 6 9
Nucleus 0 0 0 0 0 1 1 1 1 0 4 1 0 1 2 3 5 3 0 1 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0 1 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 1 0 2 0 0 0 2 1 0 1 0 0 0 6 7
Vac/Vac Membrane 0 0 0 0 0 0 1 0 0 0 2 1 1 0 1 10 22 9 7 1 0 3 6 11
Unique Cell Count 233 240 119 41 134 168 160 227 109 126 165 156 473 516 520 569 593 512 245 243 118 245 312 365
Labelled Cell Count 243 245 129 45 145 207 207 281 139 164 219 201 476 524 524 616 644 546 245 243 118 245 312 365


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 4.6 4.3 4.2 3.6 3.0 3.4 3.4 3.5 3.1 3.2 6.1 5.8 5.8 5.8 7.0 6.4 5.0 4.8 5.6
Std Deviation (1e-4) 0.7 0.7 0.8 0.6 1.3 0.8 0.7 1.0 0.8 1.8 0.9 1.3 0.9 1.1 1.2 1.2 1.4 1.1 0.9 1.0 1.3
Intensity Change (Log2) -0.1 -0.14 -0.38 -0.65 -0.45 -0.46 -0.42 -0.57 -0.55 0.39 0.33 0.31 0.31 0.58 0.47 0.1 0.05 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -0.3 -1.9 -4.1 -3.3 -4.5 -5.3 -7.3 -5.2 4.4 2.8 3.1 2.0 0.6 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.055 0.014 0.0432 0.0083 0.0137 0.0116
Bud 0.002 0.0008 0.0009 0.0003 0.0012 0.0068
Bud Neck 0.0116 0.0003 0.0005 0.0005 0.0008 0.0003
Bud Periphery 0.0021 0.0005 0.0013 0.0002 0.0019 0.0061
Bud Site 0.0057 0.0087 0.0082 0.0009 0.0038 0.0002
Cell Periphery 0.0005 0.0003 0.001 0.0001 0.0004 0
Cytoplasm 0.3779 0.6194 0.567 0.5929 0.4579 0.68
Cytoplasmic Foci 0.0422 0.018 0.0173 0.0419 0.0435 0.0093
Eisosomes 0.0003 0.0001 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.007 0.0035 0.0035 0.0025 0.0024 0.0013
Endosome 0.0265 0.006 0.0219 0.0283 0.0361 0.0054
Golgi 0.008 0.0055 0.0145 0.0131 0.023 0.0044
Lipid Particles 0.0312 0.0006 0.0012 0.0066 0.0566 0.0026
Mitochondria 0.0072 0.0007 0.0067 0.0012 0.0124 0.0024
None 0.3736 0.3108 0.3012 0.2861 0.3289 0.2616
Nuclear Periphery 0.0155 0.0009 0.0006 0.0017 0.001 0.0004
Nucleolus 0.0009 0.0001 0.0001 0.0001 0.0002 0
Nucleus 0.0079 0.001 0.0007 0.0015 0.0006 0.0009
Peroxisomes 0.0091 0.0062 0.0062 0.0085 0.012 0.0055
Punctate Nuclear 0.0084 0.0009 0.0004 0.0013 0.0013 0.0004
Vacuole 0.0039 0.0014 0.0031 0.0034 0.0015 0.0006
Vacuole Periphery 0.0034 0.0002 0.0003 0.0006 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.889 18.4786 13.7119 16.0096 15.2488 18.5224 20.9386 18.698 20.2013 16.7878
Translational Efficiency 1.4065 1.4617 1.3812 1.0335 1.1269 1.4083 1.3002 1.1707 0.968 1.1513

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1479 657 1229 86 369 1027 711 738 1848 1684 1940 824

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 735.41 687.44 941.18 1041.69 818.38 836.55 1015.61 962.77 751.98 778.38 968.46 971.01
Standard Deviation 85.83 115.37 128.71 124.58 88.01 117.36 124.75 159.63 92.43 137.42 132.23 158.19
Intensity Change Log 2 -0.097315 0.355922 0.502305 0.031681 0.311504 0.234420 -0.027938 0.332697 0.367422

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000156 0.000252 0.000183 0.003444 0.000902 0.000794 0.000143 0.001128 0.000305 0.000583 0.000168 0.001370
Bud Neck 0.007421 0.013906 0.002499 0.016576 0.004715 0.015819 0.002441 0.003008 0.006880 0.015073 0.002478 0.004424
Bud Site 0.003779 0.019961 0.009931 0.070813 0.007006 0.023024 0.010465 0.016312 0.004423 0.021829 0.010127 0.022000
Cell Periphery 0.000121 0.000094 0.000124 0.000282 0.000182 0.000088 0.000082 0.000442 0.000133 0.000090 0.000109 0.000425
Cytoplasm 0.638646* 0.595487* 0.651777* 0.174750* 0.572679* 0.592274* 0.671672* 0.626123* 0.625474* 0.593527* 0.659068* 0.579014*
Cytoplasmic Foci 0.131461 0.137856 0.025679 0.098864 0.239815* 0.161273 0.011585 0.036094 0.153097 0.152137 0.020513 0.042646
Eisosomes 0.000085 0.000056 0.000039 0.000080 0.000131 0.000065 0.000024 0.000027 0.000094 0.000061 0.000034 0.000033
Endoplasmic Reticulum 0.000652 0.000632 0.001085 0.004616 0.001110 0.000591 0.001016 0.000701 0.000743 0.000607 0.001060 0.001110
Endosome 0.007131 0.024980 0.003141 0.060797 0.012331 0.018457 0.002623 0.020281 0.008170 0.021002 0.002951 0.024509
Golgi 0.000928 0.001845 0.001073 0.065265 0.001093 0.004845 0.000192 0.012646 0.000961 0.003675 0.000750 0.018138
Lipid Particles 0.003205 0.002842 0.001275 0.003745 0.003601 0.001966 0.000808 0.000726 0.003284 0.002308 0.001104 0.001041
Mitochondria 0.001195 0.002075 0.000365 0.019872 0.000364 0.002041 0.000369 0.004354 0.001029 0.002054 0.000366 0.005974
Mitotic Spindle 0.000663 0.002485 0.011513 0.172668* 0.000502 0.013764 0.023968 0.035698 0.000631 0.009364 0.016077 0.049993
None 0.007446 0.008013 0.006055 0.002688 0.000823 0.001357 0.009063 0.029742 0.006123 0.003954 0.007157 0.026919
Nuclear Periphery 0.000302 0.000269 0.000785 0.001062 0.000229 0.000216 0.000959 0.000388 0.000287 0.000237 0.000849 0.000459
Nuclear Periphery Foci 0.000346 0.001288 0.001552 0.006122 0.001498 0.000645 0.001893 0.002099 0.000576 0.000896 0.001677 0.002519
Nucleolus 0.000804 0.004062 0.000387 0.000829 0.000368 0.000384 0.000456 0.000431 0.000717 0.001819 0.000412 0.000473
Nucleus 0.128099 0.056796 0.184655* 0.068130 0.072729 0.041314 0.117898 0.080209 0.117043 0.047354 0.160189* 0.078948
Peroxisomes 0.001351 0.005323 0.000372 0.022271 0.001617 0.006373 0.000160 0.003630 0.001404 0.005964 0.000294 0.005575
Vacuole 0.065698 0.119270 0.097362 0.198187 0.077259 0.113061 0.142496 0.123779 0.068006 0.115484 0.113904 0.131545
Vacuole Periphery 0.000513 0.002509 0.000148 0.008940 0.001044 0.001649 0.001688 0.002181 0.000619 0.001984 0.000712 0.002886

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.21 -0.64 -2.37 -2.08 -2.27 0.18 0.97 0.35 0.34 -3.06 -1.11 0.54 -1.81 -0.46 -3.50
Bud Neck -3.57 5.32 0.66 3.21 -2.12 -6.36 1.82 1.03 8.47 -1.51 -6.66 6.08 4.38 9.85 -1.79
Bud Site -6.61 -2.64 -3.93 -1.22 -3.16 -6.30 -0.19 -2.30 4.27 -2.32 -10.86 -2.85 -5.82 3.63 -3.24
Cell Periphery 3.89 1.46 -0.25 -1.96 -0.93 6.54 7.21 0.23 -0.88 -0.97 6.89 5.32 -0.34 -0.91 -0.79
Cytoplasm 4.14 5.48 13.25 10.81 11.18 -0.74 6.01 5.51 7.06 -0.47 4.64 9.64 13.96 9.82 6.85
Cytoplasmic Foci -0.49 28.43 10.96 9.91 -3.08 7.75 25.38 23.48 23.79 -6.11 0.73 36.84 31.40 28.08 -3.41
Eisosomes 5.65 11.08 5.27 0.51 -2.61 5.95 8.60 9.05 8.30 2.16 7.53 14.31 15.09 8.87 1.95
Endoplasmic Reticulum -0.03 -6.49 -2.20 -2.21 -0.33 1.90 -2.46 1.10 -1.58 6.24 0.85 -7.30 -1.66 -2.66 6.32
Endosome -7.23 7.46 -1.73 2.29 -2.91 -2.77 9.44 2.77 4.82 -3.41 -8.71 11.10 0.11 6.31 -3.87
Golgi -3.12 0.54 -1.24 -1.12 -1.27 -4.05 5.85 -1.82 0.73 -2.59 -4.68 1.46 -2.29 -0.42 -2.60
Lipid Particles 0.86 4.97 0.46 -0.36 -4.10 3.56 6.83 7.24 2.85 1.85 2.65 6.51 6.55 4.21 0.37
Mitochondria -1.30 2.30 -0.93 -0.52 -1.41 -4.31 -0.48 -1.38 0.83 -1.32 -2.26 2.24 -0.63 0.67 -1.72
Mitotic Spindle -3.48 -4.79 -4.33 -4.25 -3.96 -4.86 -5.26 -7.64 -4.46 -3.62 -5.27 -7.06 -8.68 -6.73 -6.09
None -0.06 1.84 1.82 1.52 0.54 -1.24 -8.43 -5.42 -5.18 -3.19 1.87 0.65 -2.98 -4.07 -3.51
Nuclear Periphery 0.43 -14.96 -6.58 -6.75 -1.61 0.05 -17.65 -13.43 -13.56 7.55 1.09 -21.42 -13.27 -15.00 4.53
Nuclear Periphery Foci -2.31 -11.77 -4.27 -3.27 -3.11 3.08 -2.53 -4.15 -7.08 -0.22 -1.80 -8.34 -9.18 -6.41 -2.92
Nucleolus -2.93 4.58 2.34 3.22 -1.30 -0.22 -0.03 -1.66 -1.64 -1.65 -2.52 4.33 -1.04 0.81 -1.74
Nucleus 13.42 -11.41 6.76 -0.04 12.88 5.09 -12.01 -6.80 -13.06 5.70 18.40 -14.41 -0.21 -12.10 10.94
Peroxisomes -4.35 3.43 -1.21 0.31 -1.66 -6.02 5.54 0.00 5.54 -3.57 -7.36 4.64 -0.95 5.49 -3.69
Vacuole -9.27 -13.58 -10.86 -8.74 -8.26 -6.91 -17.81 -17.04 -12.87 0.87 -13.02 -22.13 -21.39 -14.93 -7.80
Vacuole Periphery -5.22 9.95 -0.92 0.90 -1.32 -1.13 1.10 0.87 2.31 -0.39 -5.30 2.33 -0.46 3.31 -1.67