Standard name
Human Ortholog
Description Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.94 0.9 0.89 0.9 0.7 0.69 0.65 – 0.55 0.49 0.52 0.98 0.98 0.97 – 0.89 0.88 0.57 0.7 0.4 0.45 0.57 0.54
Endoplasmic Reticulum 0 0.05 0.05 0 0 0 0 0 – 0 0 0 0 0 0 – 0.09 0 0.18 0.11 0.26 0.17 0.13 0.15
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0.16 0.12 0.48 0.51 0.46 – 0.69 0.69 0.64 0 0 0 – 0.1 0.07 0.12 0.07 0.17 0.19 0.13 0.09
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 0 0 – 0 0 1 0 0 0 – 3 2 5 12 18 8 10 9
Bud 2 3 1 3 1 4 7 11 – 10 19 32 0 0 0 – 1 1 3 6 6 4 9 9
Bud Neck 0 2 2 0 0 1 1 3 – 2 1 1 0 1 0 – 6 2 4 8 7 4 4 7
Bud Site 0 0 0 0 1 2 1 1 – 4 0 2 0 0 0 – 0 1 – – – – – –
Cell Periphery 10 5 4 3 2 0 6 7 – 5 14 12 1 1 2 – 0 5 3 11 9 6 12 11
Cytoplasm 274 559 390 287 332 329 342 406 – 268 338 360 188 188 186 – 161 117 192 441 187 94 195 180
Endoplasmic Reticulum 7 28 20 4 0 7 3 2 – 1 9 5 3 3 5 – 17 5 61 67 119 36 44 50
Endosome 0 0 4 0 11 1 2 3 – 0 0 1 0 0 0 – 4 3 6 3 5 2 2 2
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 1 2 0 1 5
Mitochondria 21 12 17 52 44 225 251 290 – 337 471 442 0 0 0 – 19 9 41 43 80 38 45 30
Nucleus 1 0 0 2 0 1 2 8 – 3 2 2 0 0 0 – 2 2 0 0 0 0 0 1
Nuclear Periphery 2 1 2 0 0 0 0 0 – 2 3 1 0 0 0 – 0 0 0 1 1 0 0 1
Nucleolus 1 0 1 0 0 0 2 1 – 0 0 1 1 1 0 – 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 2 2 3 6 5 – 5 5 12 0 0 0 – 2 2 4 3 6 1 4 4
Vac/Vac Membrane 10 5 7 7 18 8 16 12 – 15 14 13 1 2 1 – 2 3 10 15 11 5 10 15
Unique Cell Count 301 596 433 321 370 467 496 629 490 685 693 192 192 192 181 133 340 630 469 208 345 335
Labelled Cell Count 330 616 451 360 412 581 639 749 652 876 886 194 196 194 217 152 340 630 469 208 345 335


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.1 4.0 3.6 3.8 3.0 3.0 3.3 – 2.8 2.8 3.1 5.6 5.6 5.6 4.8 5.1 5.9 5.6 5.7 6.3
Std Deviation (1e-4) 0.8 0.7 1.3 1.1 1.8 1.2 1.4 1.1 – 0.9 0.8 0.9 1.2 1.2 1.0 1.4 1.2 1.7 2.2 2.1 2.9
Intensity Change (Log2) – – – -0.17 -0.1 -0.45 -0.41 -0.31 – -0.54 -0.51 -0.41 0.47 0.47 0.46 0.25 0.33 0.54 0.48 0.5 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8806 1.2084 1.0338 0.8952 1.1424 1.0188 1.3933 3.0669 2.9268 2.4407 2.344 2.4803 2.0563 2.583 2.242 2.7086 2.0899 2.6439
Actin 0.0076 0.0008 0.0037 0.0047 0.0025 0.0009 0.015 0.0199 0.0129 0.0032 0.0052 0.0026 0.0044 0.0012 0.0018 0.0021 0.0151 0.0011
Bud 0.0003 0.0036 0.0018 0.0015 0.0005 0.0004 0.0003 0.001 0.0017 0.0101 0.0003 0.0007 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.002 0.0004 0.001 0.0004 0.0009 0.0068 0.0002 0.0128 0.0005 0.0046 0.0008 0.0017 0.0015 0.0002 0.002 0.0011 0.0002 0.0014
Bud Periphery 0.0006 0.0035 0.0046 0.002 0.001 0.0009 0.0023 0.0024 0.014 0.0456 0.0007 0.0021 0.0007 0.0001 0.0004 0.0009 0.0002 0.0001
Bud Site 0.0017 0.0095 0.0079 0.0043 0.001 0.0005 0.0005 0.0195 0.0087 0.0372 0.0017 0.0033 0.0007 0.0017 0.004 0.0013 0.0002 0.0002
Cell Periphery 0.0003 0.0008 0.0013 0.0002 0.0003 0.0002 0.0006 0.0017 0.001 0.0024 0.0001 0.0003 0.001 0.0007 0.0008 0.0013 0.0003 0.0004
Cytoplasm 0.1101 0.1619 0.1477 0.1763 0.1009 0.1424 0.1231 0.1936 0.1416 0.2018 0.1431 0.2348 0.2077 0.2201 0.2425 0.1559 0.2273 0.2691
Cytoplasmic Foci 0.0175 0.0057 0.0192 0.0094 0.0045 0.0089 0.0042 0.0145 0.0093 0.0116 0.0347 0.0131 0.0073 0.0068 0.0101 0.0268 0.0052 0.0118
Eisosomes 0.0003 0.0002 0.0006 0.0002 0.0003 0.0001 0.0002 0.0007 0.0004 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0075 0.0072 0.0154 0.0136 0.0097 0.0028 0.0107 0.0125 0.0087 0.0061 0.0007 0.0066 0.0326 0.0206 0.0133 0.0183 0.0257 0.0218
Endosome 0.0078 0.0049 0.0055 0.0097 0.0099 0.0031 0.001 0.0045 0.0028 0.0143 0.004 0.0031 0.008 0.0031 0.0047 0.0056 0.0038 0.003
Golgi 0.001 0.0003 0.0011 0.0018 0.0015 0.0004 0.0015 0.0016 0.001 0.0024 0.0068 0.0007 0.0012 0.0005 0.0005 0.0014 0.0045 0.0003
Lipid Particles 0.0023 0.0008 0.0021 0.0017 0.0021 0.0015 0.0022 0.0016 0.0016 0.0045 0.017 0.0008 0.0047 0.0012 0.0022 0.002 0.0025 0.0008
Mitochondria 0.0015 0.0006 0.0029 0.0042 0.0064 0.0011 0.0011 0.001 0.0195 0.0017 0.0189 0.0078 0.0006 0.0003 0.0008 0.0003 0.002 0.0005
None 0.8105 0.778 0.7602 0.7537 0.8009 0.8186 0.826 0.7027 0.7668 0.6063 0.6972 0.7114 0.7188 0.7391 0.7007 0.7744 0.7073 0.6809
Nuclear Periphery 0.006 0.0028 0.0043 0.0029 0.0053 0.0013 0.0015 0.0005 0.0015 0.0026 0.0002 0.0005 0.0022 0.0009 0.001 0.0009 0.0007 0.0015
Nucleolus 0.0013 0.001 0.0013 0.0008 0.0018 0.0007 0.0001 0.0003 0.0003 0.0034 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0004
Nucleus 0.0056 0.0086 0.0047 0.0037 0.0417 0.0017 0.0005 0.0006 0.0014 0.0052 0.0002 0.0005 0.0015 0.0011 0.0013 0.0007 0.0006 0.0018
Peroxisomes 0.0028 0.0002 0.0039 0.0035 0.0008 0.0009 0.0074 0.0022 0.0036 0.0022 0.0665 0.0086 0.0031 0.0001 0.0101 0.0032 0.0027 0.001
Punctate Nuclear 0.0095 0.0066 0.0084 0.0033 0.0024 0.0049 0.0004 0.0011 0.0011 0.0207 0.0009 0.0006 0.0011 0.0005 0.002 0.0026 0.0006 0.0028
Vacuole 0.0029 0.0022 0.002 0.0018 0.0039 0.0013 0.001 0.005 0.0012 0.0126 0.0004 0.0005 0.002 0.0012 0.0012 0.0008 0.0008 0.0008
Vacuole Periphery 0.0008 0.0003 0.0005 0.0005 0.0019 0.0004 0.0002 0.0003 0.0005 0.0014 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7963 15.0342 21.827 15.9635 15.1688 19.7319 17.5143 21.6346 19.2099 22.0033
Translational Efficiency 0.9319 0.6586 0.5146 0.6786 0.767 0.5286 0.5047 0.4943 0.5687 0.5515

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 952 1747 1010 1677 1215 1361 1341 1763 2167 3108 2351

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 838.31 719.48 815.68 841.61 641.57 726.17 819.23 816.64 651.17 723.23 817.23 827.37
Standard Deviation 134.27 94.94 95.66 142.89 78.14 97.09 102.34 115.19 92.10 96.21 98.66 128.42
Intensity Change Log 2 — -0.220529 -0.039481 0.005668 — 0.178701 0.352662 0.348094 — -0.033762 0.143731 0.164181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001124 0.000515 0.000063 0.002799 0.000293 0.000501 0.000513 0.000745 0.000333 0.000507 0.000260 0.001628
Bud Neck 0.004614 0.021488 0.004555 0.007731 0.017897 0.042874 0.007045 0.007816 0.017249 0.033479 0.005646 0.007780
Bud Site 0.033303 0.007035 0.007228 0.067995 0.005198 0.011204 0.007659 0.024909 0.006569 0.009372 0.007416 0.043419
Cell Periphery 0.000556 0.000218 0.000248 0.000434 0.000356 0.000478 0.000261 0.000290 0.000366 0.000364 0.000254 0.000352
Cytoplasm 0.286528* 0.395277* 0.434901* 0.419606* 0.397531* 0.328077* 0.390773* 0.466694* 0.392116* 0.357599* 0.415577* 0.446465*
Cytoplasmic Foci 0.121138 0.283916* 0.017161 0.046269 0.291934* 0.270910* 0.018102 0.057003 0.283602* 0.276623* 0.017573 0.052392
Eisosomes 0.000294 0.000226 0.000043 0.000102 0.000553 0.000339 0.000065 0.000157 0.000540 0.000289 0.000053 0.000133
Endoplasmic Reticulum 0.007961 0.004986 0.001095 0.004001 0.016127 0.003007 0.009332 0.006208 0.015728 0.003876 0.004702 0.005260
Endosome 0.034597 0.019894 0.001148 0.013830 0.016133 0.039681 0.002617 0.005323 0.017034 0.030988 0.001791 0.008978
Golgi 0.028454 0.005347 0.000042 0.008287 0.006192 0.008367 0.000088 0.002361 0.007278 0.007040 0.000062 0.004907
Lipid Particles 0.013575 0.014616 0.001051 0.003702 0.046256 0.024588 0.003004 0.005574 0.044662 0.020207 0.001906 0.004770
Mitochondria 0.015507 0.008695 0.000355 0.001868 0.009515 0.021206 0.000470 0.001348 0.009807 0.015710 0.000405 0.001571
Mitotic Spindle 0.002516 0.002614 0.000704 0.054532 0.001143 0.005213 0.004321 0.011662 0.001210 0.004071 0.002288 0.030079
None 0.001207 0.004878 0.007523 0.006561 0.003430 0.002420 0.002758 0.005021 0.003322 0.003499 0.005436 0.005682
Nuclear Periphery 0.002335 0.000842 0.001490 0.001619 0.001438 0.001497 0.003451 0.002179 0.001481 0.001209 0.002349 0.001938
Nuclear Periphery Foci 0.004975 0.001348 0.000500 0.005695 0.000801 0.000536 0.004971 0.004324 0.001004 0.000893 0.002458 0.004913
Nucleolus 0.004588 0.001511 0.000503 0.000594 0.000942 0.001805 0.000379 0.000436 0.001120 0.001676 0.000448 0.000504
Nucleus 0.108475 0.141734* 0.498583* 0.133959* 0.124780 0.123928 0.469324* 0.252476* 0.123985 0.131750* 0.485771* 0.201561*
Peroxisomes 0.003497 0.013725 0.000125 0.002031 0.009329 0.023317 0.000567 0.001187 0.009045 0.019103 0.000319 0.001550
Vacuole 0.322714* 0.069135 0.022530 0.215020* 0.048648 0.086159 0.074172 0.142073 0.062017 0.078680 0.045144 0.173411
Vacuole Periphery 0.002041 0.002000 0.000150 0.003361 0.001504 0.003894 0.000128 0.002215 0.001530 0.003062 0.000141 0.002707

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.91 1.93 -0.98 -2.62 -4.30 -2.14 -1.65 -1.61 -0.38 0.12 -1.60 0.24 -3.61 -2.43 -3.60
Bud Neck -7.02 -0.54 -1.44 7.03 -1.76 -8.37 7.41 7.60 12.55 0.05 -7.64 9.48 8.52 14.27 -1.07
Bud Site 1.71 1.69 -0.24 -6.75 -6.54 -4.45 -1.26 -5.53 -2.91 -4.64 -2.33 -0.45 -8.04 -7.02 -7.90
Cell Periphery 3.00 2.73 2.29 -1.31 -0.71 -0.49 1.26 1.39 1.07 0.65 0.00 1.42 1.33 0.88 -0.31
Cytoplasm -3.94 -5.00 -2.26 3.91 6.87 7.44 6.05 -0.37 -7.05 -5.78 4.38 0.64 1.78 -2.58 1.29
Cytoplasmic Foci -12.14 7.35 6.35 35.04 -5.59 2.56 52.48 44.16 34.81 -12.89 0.92 52.37 46.65 49.19 -13.58
Eisosomes 1.22 6.04 4.29 7.08 -3.88 4.61 11.60 9.55 7.70 -3.69 6.05 12.29 10.34 10.05 -5.63
Endoplasmic Reticulum 0.93 2.55 1.35 0.65 -9.75 6.72 3.18 5.39 -2.25 3.86 6.33 5.92 5.95 -1.22 0.33
Endosome 1.19 3.61 2.84 8.10 -6.54 -9.36 15.51 12.82 14.90 -4.68 -8.25 16.55 11.30 16.84 -8.44
Golgi 1.51 1.97 1.72 1.73 -3.23 -2.03 13.76 7.13 6.54 -2.53 0.05 9.83 5.78 5.88 -4.12
Lipid Particles -0.70 3.75 3.11 11.04 -8.03 7.34 18.05 17.35 11.55 -3.38 9.66 18.67 17.79 15.05 -7.54
Mitochondria 0.35 1.08 1.02 4.89 -5.57 -4.50 7.43 6.94 8.89 -1.76 -3.06 7.25 6.76 10.11 -3.73
Mitotic Spindle -0.31 1.54 -7.24 -7.07 -7.75 -2.60 -2.52 -5.07 -2.42 -2.79 -2.64 -1.62 -8.92 -7.12 -8.30
None -2.09 -4.22 -2.00 0.43 2.19 1.15 1.26 -0.45 -1.61 -1.79 -0.16 -2.05 -0.72 -0.55 1.38
Nuclear Periphery 1.64 0.61 -0.86 -7.44 -5.19 -0.14 -8.42 -4.02 -2.17 4.99 0.86 -5.30 -5.11 -5.14 -0.11
Nuclear Periphery Foci 2.31 2.93 0.33 -3.99 -6.18 1.51 -5.71 -5.09 -5.36 1.38 0.40 -4.36 -7.07 -6.57 -2.89
Nucleolus 0.77 1.10 1.03 3.33 -1.30 -4.60 5.20 3.64 7.40 -1.50 -2.94 3.52 2.49 7.65 -1.68
Nucleus -1.96 -19.51 -3.12 -2.96 37.27 0.07 -43.51 -18.64 -16.90 22.25 -1.81 -62.42 -17.05 -14.99 40.18
Peroxisomes -5.26 2.24 1.63 8.26 -3.71 -6.26 12.36 11.97 10.58 -1.28 -6.59 13.09 12.11 13.25 -3.82
Vacuole 8.86 10.20 0.49 -27.64 -33.24 -9.13 -12.62 -24.51 -17.40 -13.88 -4.06 0.24 -32.88 -31.10 -34.24
Vacuole Periphery -0.38 2.67 0.54 1.03 -3.49 -4.67 11.32 0.33 3.31 -1.99 -3.95 11.47 0.52 3.31 -3.18
Standard name
Human Ortholog
Description Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
Localization
Cell Percentages ER (16%), cytoplasm (10%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cne1

Cne1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cne1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available