Standard name
Description Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.94 0.90 0.89 0.90 0.70 0.69 0.65 0.55 0.49 0.52 0.98 0.98 0.97 0.89 0.88 0.57 0.70 0.40 0.45 0.57 0.54
Endoplasmic Reticulum 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.18 0.11 0.26 0.17 0.13 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0.16 0.12 0.48 0.51 0.46 0.69 0.69 0.64 0 0 0 0.10 0.07 0.12 0.07 0.17 0.19 0.13 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 0 0 0 0 1 0 0 0 3 2 5 12 18 8 10 9
Bud 2 3 1 3 1 4 7 11 10 19 32 0 0 0 1 1 3 6 6 4 9 9
Bud Neck 0 2 2 0 0 1 1 3 2 1 1 0 1 0 6 2 4 8 7 4 4 7
Bud Site 0 0 0 0 1 2 1 1 4 0 2 0 0 0 0 1
Cell Periphery 10 5 4 3 2 0 6 7 5 14 12 1 1 2 0 5 3 11 9 6 12 11
Cytoplasm 274 559 390 287 332 329 342 406 268 338 360 188 188 186 161 117 192 441 187 94 195 180
Endoplasmic Reticulum 7 28 20 4 0 7 3 2 1 9 5 3 3 5 17 5 61 67 119 36 44 50
Endosome 0 0 4 0 11 1 2 3 0 0 1 0 0 0 4 3 6 3 5 2 2 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 5
Mitochondria 21 12 17 52 44 225 251 290 337 471 442 0 0 0 19 9 41 43 80 38 45 30
Nucleus 1 0 0 2 0 1 2 8 3 2 2 0 0 0 2 2 0 0 0 0 0 1
Nuclear Periphery 2 1 2 0 0 0 0 0 2 3 1 0 0 0 0 0 0 1 1 0 0 1
Nucleolus 1 0 1 0 0 0 2 1 0 0 1 1 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 2 2 3 6 5 5 5 12 0 0 0 2 2 4 3 6 1 4 4
Vac/Vac Membrane 10 5 7 7 18 8 16 12 15 14 13 1 2 1 2 3 10 15 11 5 10 15
Unique Cell Count 301 596 433 321 370 467 496 629 490 685 693 192 192 192 181 133 340 630 469 208 345 335
Labelled Cell Count 330 616 451 360 412 581 639 749 652 876 886 194 196 194 217 152 340 630 469 208 345 335


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.1 4.0 3.6 3.8 3.0 3.0 3.3 2.8 2.8 3.1 5.6 5.6 5.6 4.8 5.1 5.9 5.6 5.7 6.3
Std Deviation (1e-4) 0.8 0.7 1.3 1.1 1.8 1.2 1.4 1.1 0.9 0.8 0.9 1.2 1.2 1.0 1.4 1.2 1.7 2.2 2.1 2.9
Intensity Change (Log2) -0.17 -0.1 -0.45 -0.41 -0.31 -0.54 -0.51 -0.41 0.47 0.47 0.46 0.25 0.33 0.54 0.48 0.5 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole