Standard name
Description CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.7 0.82 0.89 0.84 0.95 0.86 0.91 0.88 0.75 0.69 0.76 0.73 0.84 0.91 0.98 0.83 0.87 0.81 0.46 0.6 0.6 0.56 0.66 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.19 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.14 0 0.18 0.12 0.14 0.31 0.37 0.37 0.34 0 0 0 0 0.1 0 0 0 0.05 0.07 0.06 0
Nucleus 0.18 0.11 0.06 0.11 0 0 0 0 0.05 0.07 0 0 0.07 0 0 0 0 0.06 0.2 0.12 0.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.06 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.06 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0.09 0.05 0.06 0.08 0.07 0.1 0.13 0.11 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3 2 9 11 12
Bud 0 1 0 0 0 2 3 1 6 5 7 2 3 0 1 0 0 0 24 15 1 20 11 17
Bud Neck 0 3 3 0 2 1 3 6 5 3 0 7 0 0 0 1 0 0 5 5 0 8 3 12
Bud Site 0 0 0 0 0 0 1 0 1 2 3 3 0 0 0 0 0 0
Cell Periphery 1 2 2 1 0 0 0 0 0 0 1 0 1 0 0 0 0 1 1 0 2 1 1 2
Cytoplasm 227 248 126 53 145 81 190 130 122 131 147 124 369 431 549 53 34 13 163 190 85 181 245 229
Endoplasmic Reticulum 2 1 0 0 0 0 0 0 0 0 0 0 0 1 2 5 3 3 0 0 2 5 6 4
Endosome 1 0 0 0 0 0 0 0 1 0 0 1 2 2 2 4 0 1 7 2 0 4 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 2 5 1
Mitochondria 19 6 0 9 2 17 25 21 51 70 72 57 17 7 4 2 4 0 12 9 6 22 22 13
Nucleus 59 34 9 7 4 2 5 5 8 13 5 6 31 15 0 1 0 1 69 37 13 11 6 17
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 2 0 1 0 0 0 0 0 0 2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 1 0 0 0 2 1 0 3 1 1 0 0 1 1 2 0 28 18 4 5 7 11
Vac/Vac Membrane 41 29 9 0 2 1 2 2 3 3 2 5 38 39 11 6 2 1 27 22 13 42 40 62
Unique Cell Count 323 304 141 63 152 94 209 148 162 189 193 169 440 472 561 64 39 16 353 319 142 325 372 400
Labelled Cell Count 352 326 150 70 155 104 231 168 197 231 240 206 462 496 572 76 45 20 353 319 142 325 372 400


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 5.1 4.1 4.8 3.6 4.0 4.0 3.4 3.2 2.9 3.4 4.2 4.2 4.2 5.9 5.5 5.5 4.9 4.9 5.0
Std Deviation (1e-4) 0.9 1.0 1.6 1.2 1.6 1.7 1.5 1.3 1.2 1.2 0.8 1.3 1.1 1.5 1.3 1.7 1.7 1.5 1.7 1.3 1.3
Intensity Change (Log2) -0.32 -0.11 -0.53 -0.37 -0.35 -0.6 -0.69 -0.81 -0.58 -0.3 -0.29 -0.29 0.21 0.1 0.1 -0.07 -0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Far1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available